MD04G1088900.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002293
Physical Location & Seq
Forward (+)
15414960 .. 15417693
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1088900.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGGTTCTTCAGGGCAGAGCAGCAGCAACTACGATCTGTCGTTCAAGATCTTGCTGATAGGAGACTCCGCCGTCGGCAAAAGTAGTCTGCTTGTTAGCTTCATCTCCAACTCTGTTGACGATCTTGCCCCCACCATTGGTGTGGATTTTAAAATCAAGCTGCTCACTGTAGCTGGGAAGAAAATCAAGATGACTATTTGGGACACAGCTGGACAGGAGAGGTTCAGAACATTAACAAGCTCTTACTACAGAAATGCCCAAGGAATCATCCTTGTGTATGATGTGACTCGGAGAGATACCTTTACGAACTTATCGGATGTGTGGGCTAAAGAAGTGGAACTGTACTCGACTAACCAGGACTGTGTCAAGATGCTTGTTGGGAATAAAGTTGATAGAGAATCTGAAAGGGCTGTAAGTAGAGAAGAGGGGATTTCTCTTGCAAAAGATCTCGGATGTTTGTTTCTTGAATGTAGCGCTAAAACTCGAGAAAATGTGGAGCACTGCTTCGAAGAGCTAGCGTTGAAGATAATGGAGGTTCCTAGTCTCTTGGAAGAAGGTTCGACCGTGGCAAAGAGAAATATATTGAAGCAGAAACAGGAAAATCAAGCACCTCCTATCGGTAGTTGTTGCTCGTAA

Protein Analysis

212

Amino Acids

23.33

Weight (kDa)

5.62

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 12 - 173 5e-19 ADP-ribosylation factor family
Ras PF00071 16 - 177 1.3e-56 Ras family
Roc PF08477 16 - 131 2.6e-31 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 16 - 104 5.1e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 16 - 136 6.2e-06 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AciI CCGC 1 cut(s) 69
AfaI GTAC 1 cut(s) 344
AfeI AGCGCT 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 137, 617
AgsI TTSAA 4 cut(s) 46, 467, 523, 586
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 99, 160, 173, 209, 240, 514
AluI AGCT 6 cut(s) 99, 160, 173, 209, 240, 514
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 2 cut(s) 57, 548
Ama87I CYCGRG 1 cut(s) 483
Aor51HI AGCGCT 1 cut(s) 475
ApeKI GCWGC 3 cut(s) 21, 24, 160
AspLEI GCGC 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 507
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 33, 36, 147
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 57, 548
BfaI CTAG 2 cut(s) 515, 540
BfmI CTRYAG 2 cut(s) 168, 247
BfoI RGCGCY 1 cut(s) 477
BglII AGATCT 2 cut(s) 48, 445
BisI GCNGC 3 cut(s) 22, 25, 161
BlsI GCNGC 3 cut(s) 23, 26, 162
Bme1390I CCNGG 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 360
BmtI GCTAGC 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 259, 564
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 617
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 259, 564
BseGI GGATG 3 cut(s) 267, 322, 458
BseLI CCNNNNNNNGG 2 cut(s) 137, 617
BseXI GCAGC 3 cut(s) 33, 36, 147
BseYI CCCAGC 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 483
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 137, 617
BsmAI GTCTC 2 cut(s) 57, 548
BsmFI GGGAC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 483
Bsp119I TTCGAA 1 cut(s) 507
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 4 cut(s) 34, 48, 121, 445
BspACI CCGC 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 537
BspOI GCTAGC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 507
BssECI CCNNGG 2 cut(s) 259, 564
BssMI GATC 4 cut(s) 34, 48, 121, 445
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 4 cut(s) 169, 342, 362, 565
Bst6I CTCTTC 2 cut(s) 417, 504
BstBI TTCGAA 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 564
BstF5I GGATG 3 cut(s) 267, 322, 458
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 4 cut(s) 37, 51, 124, 448
BstMAI GTCTC 2 cut(s) 57, 548
BstMBI GATC 4 cut(s) 34, 48, 121, 445
BstMCI CGRYCG 1 cut(s) 564
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 2 cut(s) 168, 247
BstV1I GCAGC 3 cut(s) 33, 36, 147
BstX2I RGATCY 2 cut(s) 48, 445
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 2 cut(s) 48, 445
BtgI CCRYGG 1 cut(s) 564
BtsCI GGATG 3 cut(s) 267, 322, 458
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 2 cut(s) 165, 499
Cac8I GCNNGC 1 cut(s) 516
CfoI GCGC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 343
CviJI RGCY 8 cut(s) 99, 160, 173, 209, 240, 326, 410, 514
CviKI_1 RGCY 8 cut(s) 99, 160, 173, 209, 240, 326, 410, 514
CviQI GTAC 1 cut(s) 343
DpnI GATC 4 cut(s) 36, 50, 123, 447
DpnII GATC 4 cut(s) 34, 48, 121, 445
DraI TTTAAA 1 cut(s) 151
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 417, 504
EarI CTCTTC 2 cut(s) 417, 504
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaiI YATR 2 cut(s) 279, 581
FaqI GGGAC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 22, 25, 161
FokI GGATG 3 cut(s) 254, 329, 465
Fsp4HI GCNGC 3 cut(s) 22, 25, 161
FspBI CTAG 2 cut(s) 515, 540
GlaI GCGC 1 cut(s) 475
GluI GCNGC 3 cut(s) 22, 25, 161
GsaI CCCAGC 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 477
HhaI GCGC 1 cut(s) 476
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 4 cut(s) 65, 264, 286, 398
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 5 cut(s) 227, 291, 316, 403, 452
Hpy188III TCNNGA 5 cut(s) 46, 187, 367, 464, 485
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 548
HpyCH4III ACNGT 4 cut(s) 169, 342, 362, 565
HpyCH4V TGCA 1 cut(s) 440
HspAI GCGC 1 cut(s) 474
Kzo9I GATC 4 cut(s) 34, 48, 121, 445
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 496
LpnPI CCDG 6 cut(s) 159, 195, 200, 341, 368, 581
Lsp1109I GCAGC 3 cut(s) 33, 36, 147
LweI GCATC 1 cut(s) 360
MaeI CTAG 2 cut(s) 515, 540
MaeIII GTNAC 1 cut(s) 283
MalI GATC 4 cut(s) 36, 50, 123, 447
MboI GATC 4 cut(s) 34, 48, 121, 445
MboII GAAGA 5 cut(s) 190, 434, 521, 535, 563
MflI RGATCY 2 cut(s) 48, 445
MhlI GDGCHC 1 cut(s) 501
MlyI GAGTC 2 cut(s) 59, 280
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 4 cut(s) 213, 418, 526, 621
MseI TTAA 2 cut(s) 150, 233
MslI CAYNNNNRTG 2 cut(s) 140, 272
MspA1I CMGCKG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
NdeII GATC 4 cut(s) 34, 48, 121, 445
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 1 cut(s) 283
NspV TTCGAA 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 264, 398
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 3 cut(s) 23, 26, 162
PleI GAGTC 2 cut(s) 59, 280
PpsI GAGTC 2 cut(s) 59, 280
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 2 cut(s) 48, 445
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 2 cut(s) 140, 272
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 2 cut(s) 150, 233
SatI GCNGC 3 cut(s) 22, 25, 161
Sau3AI GATC 4 cut(s) 34, 48, 121, 445
SchI GAGTC 2 cut(s) 59, 280
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 1 cut(s) 360
SfcI CTRYAG 2 cut(s) 168, 247
Sfr274I CTCGAG 1 cut(s) 483
SfuI TTCGAA 1 cut(s) 507
SlaI CTCGAG 1 cut(s) 483
SmiMI CAYNNNNRTG 2 cut(s) 140, 272
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SsiI CCGC 1 cut(s) 69
SspMI CTAG 2 cut(s) 515, 540
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 4 cut(s) 169, 342, 362, 565
TaqI TCGA 4 cut(s) 347, 484, 507, 560
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 2 cut(s) 264, 398
Tru1I TTAA 2 cut(s) 150, 233
Tru9I TTAA 2 cut(s) 150, 233
TscAI CASTG 2 cut(s) 172, 506
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 3 cut(s) 21, 24, 160
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 2 cut(s) 172, 506
Tth111I GACNNNGTC 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 483
XspI CTAG 2 cut(s) 515, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.