Rh1DG296400

Ras-related protein RABC2a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002293
Physical Location & Seq
Forward (+)
52191090 .. 52192693
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG296400.1

Sequence Viewer

Length: 630 bp
ATGAAGGGTTCACCAAGAAATTATGATTACTCATTCAAGATTTTGCTGGCTGGTGATTCTGGTGTTGGAAAGAGCAGTCTTCTTTTGAGCTTCATCTCCAACTTTGTTCAAGACCTTTCTCCTACAATTGGTGTGGATTTCAAGATCAAACAGCTTTCAATTGATGGGAAGAGATTGAAGCTAACAATATGGGACACAGCTGGACAGGAGAGGTTCGGAACAGTAATAAGCTCTTACTACAGAGGTGCACATGGAATCATTCTCGTGTATGATGTAACACGGCGAGAAACCTTTACAAATTTATCAAATCTGTGGGCAAAGGAAGTAGACCGGTACTCCACCAATCAAGATTGCATTAAAATTCTAGTCGGGAACAAAGTTGACAGGGACAAAGAGAGGGTTGTAACCAGAGAAGAAGGGATGGCACTTGCACAGGAGCATAAATGTTTGTTTCTTGAATGTAGTGCTAAAACTAAAGAAAATGTTCAGCAATGCTTTAAAGATCTCACATTGAAGATGCTGGAGGTACCAAGTTTACTGGAAAGTGGATCTGTGGACGTGAAGAGACAAATTTTGAAGCAGAAAGAAGTAGACCAGATGCCTTGTAGCGGCAATTGCTGCTCTCAATAG

Protein Analysis

209

Amino Acids

23.59

Weight (kDa)

8.61

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 10 - 170 2.6e-16 ADP-ribosylation factor family
Ras PF00071 13 - 173 2.4e-56 Ras family
Roc PF08477 13 - 128 2e-30 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 13 - 112 6.3e-06 50S ribosome-binding GTPase
GTP_EFTU PF00009 45 - 169 8e-07 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 526
AccB1I GGYRCC 1 cut(s) 526
AccI GTMKAC 2 cut(s) 327, 591
AciI CCGC 1 cut(s) 609
AclWI GGATC 1 cut(s) 556
AcsI RAATTY 3 cut(s) 298, 360, 570
AfaI GTAC 2 cut(s) 335, 528
AfiI CCNNNNNNNGG 2 cut(s) 128, 608
AgeI ACCGGT 1 cut(s) 330
AgsI TTSAA 8 cut(s) 37, 110, 142, 159, 178, 458, 514, 577
AjiI CACGTC 1 cut(s) 559
AluBI AGCT 5 cut(s) 90, 154, 181, 200, 231
AluI AGCT 5 cut(s) 90, 154, 181, 200, 231
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 559
Alw44I GTGCAC 1 cut(s) 246
AlwI GGATC 1 cut(s) 556
ApaLI GTGCAC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 618
ApoI RAATTY 3 cut(s) 298, 360, 570
AsiGI ACCGGT 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 526
AsuHPI GGTGA 2 cut(s) 3, 65
BaeGI GKGCMC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 526
BauI CACGAG 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 605
BccI CCATC 2 cut(s) 158, 415
BceAI ACGGC 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 559
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 238
BglII AGATCT 1 cut(s) 502
BisI GCNGC 2 cut(s) 610, 619
BlsI GCNGC 2 cut(s) 611, 620
BmgBI CACGTC 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 528
BmsI GCATC 2 cut(s) 507, 588
BpiI GAAGAC 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 608
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 497
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 128, 608
BseMI GCAATG 1 cut(s) 497
BseNI ACTGG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 605
BshNI GGYRCC 1 cut(s) 526
BshTI ACCGGT 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 331
BslFI GGGAC 2 cut(s) 206, 401
BslI CCNNNNNNNGG 2 cut(s) 128, 608
BsmAI GTCTC 1 cut(s) 559
BsmFI GGGAC 2 cut(s) 206, 401
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 3 cut(s) 144, 502, 548
BspACI CCGC 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 528
BspPI GGATC 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 330
BssMI GATC 3 cut(s) 144, 502, 548
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 164, 557
BstAPI GCANNNNNTGC 1 cut(s) 618
BstC8I GCNNGC 1 cut(s) 48
BstF5I GGATG 1 cut(s) 426
BstKTI GATC 3 cut(s) 147, 505, 551
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 3 cut(s) 144, 502, 548
BstMWI GCNNNNNNNGC 2 cut(s) 615, 618
BstSFI CTRYAG 1 cut(s) 238
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 502, 548
BstYI RGATCY 2 cut(s) 502, 548
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 330
Csp6I GTAC 2 cut(s) 334, 527
CspAI ACCGGT 1 cut(s) 330
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 1 cut(s) 251
CviJI RGCY 6 cut(s) 50, 90, 154, 181, 200, 231
CviKI_1 RGCY 6 cut(s) 50, 90, 154, 181, 200, 231
CviQI GTAC 2 cut(s) 334, 527
DpnI GATC 3 cut(s) 146, 504, 550
DpnII GATC 3 cut(s) 144, 502, 548
DraI TTTAAA 1 cut(s) 499
Eam1104I CTCTTC 2 cut(s) 164, 557
EarI CTCTTC 2 cut(s) 164, 557
FaeI CATG 1 cut(s) 254
FaiI YATR 5 cut(s) 24, 190, 252, 270, 441
FaqI GGGAC 2 cut(s) 206, 401
FatI CATG 1 cut(s) 250
FblI GTMKAC 2 cut(s) 327, 591
Fnu4HI GCNGC 2 cut(s) 610, 619
FokI GGATG 1 cut(s) 433
Fsp4HI GCNGC 2 cut(s) 610, 619
FspBI CTAG 1 cut(s) 365
GluI GCNGC 2 cut(s) 610, 619
GsuI CTGGAG 1 cut(s) 542
HapII CCGG 1 cut(s) 331
Hin1II CATG 1 cut(s) 254
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 2 cut(s) 56, 255
HpaII CCGG 1 cut(s) 331
HphI GGTGA 2 cut(s) 3, 65
Hpy166II GTNNAC 7 cut(s) 11, 248, 328, 382, 536, 556, 592
Hpy188I TCNGA 1 cut(s) 218
Hpy188III TCNNGA 6 cut(s) 37, 110, 142, 347, 370, 455
Hpy8I GTNNAC 7 cut(s) 11, 248, 328, 382, 536, 556, 592
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 3 cut(s) 248, 354, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 615, 618
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 1 cut(s) 254
KpnI GGTACC 1 cut(s) 530
Kzo9I GATC 3 cut(s) 144, 502, 548
LmnI GCTCC 1 cut(s) 436
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 2 cut(s) 507, 588
MaeI CTAG 1 cut(s) 365
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 2 cut(s) 274, 403
MalI GATC 3 cut(s) 146, 504, 550
MboI GATC 3 cut(s) 144, 502, 548
MboII GAAGA 5 cut(s) 71, 181, 425, 526, 574
MfeI CAATTG 3 cut(s) 126, 159, 613
MflI RGATCY 2 cut(s) 502, 548
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 7 cut(s) 19, 126, 159, 298, 360, 570, 613
MmeI TCCRAC 2 cut(s) 46, 123
MnlI CCTC 4 cut(s) 204, 236, 390, 517
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 2 cut(s) 357, 498
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 331
MunI CAATTG 3 cut(s) 126, 159, 613
MwoI GCNNNNNNNGC 2 cut(s) 615, 618
NdeII GATC 3 cut(s) 144, 502, 548
NlaIII CATG 1 cut(s) 254
NlaIV GGNNCC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 56, 255
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 2 cut(s) 611, 620
PspN4I GGNNCC 1 cut(s) 528
PsuI RGATCY 2 cut(s) 502, 548
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 2 cut(s) 335, 528
RsaNI GTAC 2 cut(s) 334, 527
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 2 cut(s) 357, 498
SatI GCNGC 2 cut(s) 610, 619
Sau3AI GATC 3 cut(s) 144, 502, 548
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 2 cut(s) 507, 588
SfcI CTRYAG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 7 cut(s) 19, 126, 159, 298, 360, 570, 613
SsiI CCGC 1 cut(s) 609
SspMI CTAG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 561
TasI AATT 7 cut(s) 19, 126, 159, 298, 360, 570, 613
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 2 cut(s) 56, 255
Tru1I TTAA 2 cut(s) 357, 498
Tru9I TTAA 2 cut(s) 357, 498
TseI GCWGC 1 cut(s) 618
TspDTI ATGAA 2 cut(s) 17, 82
VneI GTGCAC 1 cut(s) 246
XapI RAATTY 3 cut(s) 298, 360, 570
XmiI GTMKAC 2 cut(s) 327, 591
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.