RLG00000027678

Ras-related protein RABC2a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002293
Physical Location & Seq
Reverse (-)
13856166 .. 13857786
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027678

Sequence Viewer

Length: 636 bp
ATGAAGGGTTCACCAATTGCAAGAAATTATGATTACTCATTCAAGATTTTGCTGGCTGGTGATTCTGGTGTTGGAAAGAGCAGTCTTCTTTTGAGCTTCATCTCCAACTTTGTTCAAGACCTTTCTCCTACAATTGGTGTGGATTTCAAGATCAAACAGCTTTCAATTGATGGGAAGAGATTGAAGCTAACAATATGGGACACAGCTGGACAGGAGAGGTTTGGAACAGTAATAAGCTCTTACTATAGAGGTGCACATGGAATCATTCTCGTGTATGATGTAACACGGCGAGAAACCTTTACAAATTTATCAAATCTGTGGGCAAAGGAAGTAGACCGGTACTCCACCAATCAAGATTGCATTAAAATTCTAGTCGGGAACAAAGTTGACAGGGACAAAGAGAGGGTTGTAACCAGAGAAGAAGGGATGGCACTTGCACAGGAGCATAAATGTTTGTTTCTTGAATGTAGTGCTAAAACCAAAGAAAATGTTCAGCAATGCTTTAAAGATCTCACATTGAAGATGCTGGAGGTACCAAGTTTACTGGAAAGTGGATCTGTGGACGTGAAGAGACAAATTTTGAAGCAGAAAGAAGTAGACCAGATGCCTTGCAGCGGCAATTGCTGCTCCCAATAG

Protein Analysis

212

Amino Acids

23.77

Weight (kDa)

8.61

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 11 - 172 2.6e-16 ADP-ribosylation factor family
Ras PF00071 15 - 175 2.5e-56 Ras family
Roc PF08477 15 - 130 2e-30 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 114 6.5e-06 50S ribosome-binding GTPase
GTP_EFTU PF00009 47 - 171 8.2e-07 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 532
AccI GTMKAC 2 cut(s) 333, 597
AciI CCGC 1 cut(s) 615
AclWI GGATC 1 cut(s) 562
AcsI RAATTY 3 cut(s) 304, 366, 576
AfaI GTAC 2 cut(s) 341, 534
AfiI CCNNNNNNNGG 2 cut(s) 134, 614
AgeI ACCGGT 1 cut(s) 336
AgsI TTSAA 8 cut(s) 43, 116, 148, 165, 184, 464, 520, 583
AjiI CACGTC 1 cut(s) 565
AluBI AGCT 5 cut(s) 96, 160, 187, 206, 237
AluI AGCT 5 cut(s) 96, 160, 187, 206, 237
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 1 cut(s) 565
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 562
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 2 cut(s) 612, 624
ApoI RAATTY 3 cut(s) 304, 366, 576
AsiGI ACCGGT 1 cut(s) 336
Asp700I GAANNNNTTC 1 cut(s) 489
Asp718I GGTACC 1 cut(s) 532
AsuHPI GGTGA 2 cut(s) 3, 71
BaeGI GKGCMC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 532
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 2 cut(s) 611, 624
BccI CCATC 2 cut(s) 164, 421
BceAI ACGGC 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 244
BglII AGATCT 1 cut(s) 508
BisI GCNGC 3 cut(s) 613, 616, 625
BlsI GCNGC 3 cut(s) 614, 617, 626
BmgBI CACGTC 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 534
BmsI GCATC 2 cut(s) 513, 594
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 614
Bse118I RCCGGY 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 503
BseGI GGATG 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 134, 614
BseMI GCAATG 1 cut(s) 503
BseNI ACTGG 1 cut(s) 549
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 2 cut(s) 611, 624
BshNI GGYRCC 1 cut(s) 532
BshTI ACCGGT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 1 cut(s) 337
BslFI GGGAC 2 cut(s) 212, 407
BslI CCNNNNNNNGG 2 cut(s) 134, 614
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 2 cut(s) 212, 407
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 3 cut(s) 150, 508, 554
BspACI CCGC 1 cut(s) 615
BspLI GGNNCC 1 cut(s) 534
BspPI GGATC 1 cut(s) 562
BspT107I GGYRCC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 336
BsrI ACTGG 1 cut(s) 549
BssAI RCCGGY 1 cut(s) 336
BssMI GATC 3 cut(s) 150, 508, 554
BssSI CACGAG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 2 cut(s) 170, 563
BstAPI GCANNNNNTGC 1 cut(s) 624
BstC8I GCNNGC 1 cut(s) 54
BstF5I GGATG 1 cut(s) 432
BstKTI GATC 3 cut(s) 153, 511, 557
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 3 cut(s) 150, 508, 554
BstMWI GCNNNNNNNGC 2 cut(s) 621, 624
BstSFI CTRYAG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 611, 624
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 2 cut(s) 508, 554
BstYI RGATCY 2 cut(s) 508, 554
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 336
Csp6I GTAC 2 cut(s) 340, 533
CspAI ACCGGT 1 cut(s) 336
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 1 cut(s) 257
CviJI RGCY 6 cut(s) 56, 96, 160, 187, 206, 237
CviKI_1 RGCY 6 cut(s) 56, 96, 160, 187, 206, 237
CviQI GTAC 2 cut(s) 340, 533
DpnI GATC 3 cut(s) 152, 510, 556
DpnII GATC 3 cut(s) 150, 508, 554
DraI TTTAAA 1 cut(s) 505
Eam1104I CTCTTC 2 cut(s) 170, 563
EarI CTCTTC 2 cut(s) 170, 563
FaeI CATG 1 cut(s) 260
FaiI YATR 6 cut(s) 30, 196, 246, 258, 276, 447
FaqI GGGAC 2 cut(s) 212, 407
FatI CATG 1 cut(s) 256
FblI GTMKAC 2 cut(s) 333, 597
Fnu4HI GCNGC 3 cut(s) 613, 616, 625
FokI GGATG 1 cut(s) 439
Fsp4HI GCNGC 3 cut(s) 613, 616, 625
FspBI CTAG 1 cut(s) 371
GluI GCNGC 3 cut(s) 613, 616, 625
GsuI CTGGAG 1 cut(s) 548
HapII CCGG 1 cut(s) 337
Hin1II CATG 1 cut(s) 260
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 2 cut(s) 62, 261
HpaII CCGG 1 cut(s) 337
HphI GGTGA 2 cut(s) 3, 71
Hpy166II GTNNAC 7 cut(s) 11, 254, 334, 388, 542, 562, 598
Hpy188III TCNNGA 6 cut(s) 43, 116, 148, 353, 376, 461
Hpy8I GTNNAC 7 cut(s) 11, 254, 334, 388, 542, 562, 598
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 5 cut(s) 20, 254, 360, 437, 612
HpyF10VI GCNNNNNNNGC 2 cut(s) 621, 624
HpySE526I ACGT 1 cut(s) 564
Hsp92II CATG 1 cut(s) 260
KpnI GGTACC 1 cut(s) 536
Kzo9I GATC 3 cut(s) 150, 508, 554
LmnI GCTCC 2 cut(s) 442, 632
Lsp1109I GCAGC 2 cut(s) 611, 624
LweI GCATC 2 cut(s) 513, 594
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 280, 409
MalI GATC 3 cut(s) 152, 510, 556
MboI GATC 3 cut(s) 150, 508, 554
MboII GAAGA 5 cut(s) 77, 187, 431, 532, 580
MfeI CAATTG 4 cut(s) 15, 132, 165, 619
MflI RGATCY 2 cut(s) 508, 554
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 8 cut(s) 15, 25, 132, 165, 304, 366, 576, 619
MmeI TCCRAC 2 cut(s) 52, 129
MnlI CCTC 4 cut(s) 210, 242, 396, 523
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 2 cut(s) 363, 504
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 206, 615
MspI CCGG 1 cut(s) 337
MunI CAATTG 4 cut(s) 15, 132, 165, 619
MwoI GCNNNNNNNGC 2 cut(s) 621, 624
NdeII GATC 3 cut(s) 150, 508, 554
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 62, 261
PinAI ACCGGT 1 cut(s) 336
PkrI GCNGC 3 cut(s) 614, 617, 626
PspN4I GGNNCC 1 cut(s) 534
PsuI RGATCY 2 cut(s) 508, 554
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 341, 534
RsaNI GTAC 2 cut(s) 340, 533
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 2 cut(s) 363, 504
SatI GCNGC 3 cut(s) 613, 616, 625
Sau3AI GATC 3 cut(s) 150, 508, 554
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 2 cut(s) 513, 594
SfcI CTRYAG 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 269
Sse9I AATT 8 cut(s) 15, 25, 132, 165, 304, 366, 576, 619
SsiI CCGC 1 cut(s) 615
SspMI CTAG 1 cut(s) 371
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 567
TasI AATT 8 cut(s) 15, 25, 132, 165, 304, 366, 576, 619
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 2 cut(s) 62, 261
Tru1I TTAA 2 cut(s) 363, 504
Tru9I TTAA 2 cut(s) 363, 504
TseI GCWGC 2 cut(s) 612, 624
TspDTI ATGAA 2 cut(s) 17, 88
VneI GTGCAC 1 cut(s) 252
XapI RAATTY 3 cut(s) 304, 366, 576
XmiI GTMKAC 2 cut(s) 333, 597
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.