Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002293

Physical Location & Seq

Genomic Coordinates Forward (+)

16891458 .. 16893496

Gene Structure 2039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1106600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 636 bp

ATGGGTTCTTCAGGGCAGAGCAGCAGCAACTACGATCTGTCGTTCAAGATCTTGCTGATCGGAGACTCCGCCGTCGGCAAAAGTAGTCTGCTTGTTAGCTTCATCTCCAACTCTGTTGACGATCTTGCCCCCACCATTGGTGTAGATTTTAAAATCAAGCTTCTCACTGTAGCTGGGAAGAAAATCAAGATGACAATTTGGGACACAGCTGGACAGGAGAGGTTCAGAACGTTAACGAGCTCTTACTACAGAAATGCCCAAGGAATCATCCTCGTGTATGATGTGATCCGGAGAGACACCTTTACCAACTTATCGGATGTCTGGGCTAAAGAGGTGCAACTGTATTCGACGAACCATGACCGTGTCAAGATGCTTGTTGGGAATAAAGTTGATAGAGAATCGGAAAGGGCTGTAAGTAGAGAAGAGGGGATCTCACTTGCAAAAGATCTTGGATGTTTGTTTCTTGAATGTAGCGCTAAAACTCGAGAAAATGTGGAGCACTGCTTCGAAGAGCTAGCATTGAAGATAATGGACGTTCCTAGTCTCTTGGAAGAAGGTTCAACAGTGGCAAAGAGAAATATTTTGAAGCAGAAACGGGAAAATCAAGCACCACCTAGCGGTAGTTGTTGCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005794 GO:0008092 GO:0012505 GO:0017022 GO:0030742 GO:0032029 GO:0032036 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0080115

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ras

Protein Analysis

212

Amino Acids

23.39

Weight (kDa)

7.59

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	12 - 173	3.9e-18	ADP-ribosylation factor family
Ras	PF00071	16 - 177	1.7e-55	Ras family
Roc	PF08477	16 - 131	8.2e-31	Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1	PF01926	16 - 105	5.1e-06	50S ribosome-binding GTPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09910 AT3G09910 AT3G09910 AT3G09910 AT3G09910 AT5G03530 AT5G03530
fragaria_vesca	FvH4_6g16870 FvH4_7g18330
malus_domestica	MD01G1099300.v1.1 MD04G1088900.v1.1 MD07G1166100.v1.1 MD12G1106600.v1.1
prunus_persica	Prupe.2G205300_v2.0.a1 Prupe.7G125400_v2.0.a1
pyrus_communis	pycom04g08350 pycom07g16160 pycom12g10710
rosa_chinensis	RchiOBHm_Chr1g0362041 RchiOBHm_Chr3g0470891 RchiOBHm_Chr5g0009971 RchiOBHm_Chr5g0067631
rosa_laevigata	RLG00000024236 RLG00000027678
rosa_multiflora	Rmu_sc0010614.1_g000006
rosa_roxburghii	Rroxscaffold_4G00294550 Rroxscaffold_6G00410440
rosa_rugosa	Rorug01G0292900 Rorug01G0293000 Rorug03G0113400
rosa_samantha	Rh1AG303200 Rh1AG338800 Rh1BG266400 Rh1CG284600 Rh1DG296400 Rh3AG163200 Rh3BG188600 Rh3CG178600 Rh3DG187500
rosa_wichuraiana	Rw1G027000 Rw3G015370 Rw5G028030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	71
AccIII	TCCGGA	1 cut(s)	288
AciI	CCGC	2 cut(s)	69, 618
AclI	AACGTT	1 cut(s)	230
AclWI	GGATC	2 cut(s)	280, 437
AfeI	AGCGCT	1 cut(s)	475
AfiI	CCNNNNNNNGG	2 cut(s)	137, 617
AgsI	TTSAA	5 cut(s)	46, 467, 523, 561, 586
AluBI	AGCT	6 cut(s)	99, 160, 173, 209, 240, 514
AluI	AGCT	6 cut(s)	99, 160, 173, 209, 240, 514
Alw21I	GWGCWC	2 cut(s)	242, 501
Alw26I	GTCTC	3 cut(s)	57, 288, 548
AlwI	GGATC	2 cut(s)	280, 437
Ama87I	CYCGRG	1 cut(s)	483
Aor13HI	TCCGGA	1 cut(s)	288
Aor51HI	AGCGCT	1 cut(s)	475
ApeKI	GCWGC	2 cut(s)	21, 24
AspLEI	GCGC	1 cut(s)	476
AsuII	TTCGAA	1 cut(s)	507
AsuNHI	GCTAGC	1 cut(s)	514
AvaI	CYCGRG	1 cut(s)	483
BanII	GRGCYC	1 cut(s)	242
BauI	CACGAG	1 cut(s)	272
Bbv12I	GWGCWC	2 cut(s)	242, 501
BbvI	GCAGC	2 cut(s)	33, 36
BceAI	ACGGC	1 cut(s)	56
BcoDI	GTCTC	3 cut(s)	57, 288, 548
BfaI	CTAG	3 cut(s)	515, 540, 615
BfmI	CTRYAG	2 cut(s)	168, 247
BfoI	RGCGCY	1 cut(s)	477
BglII	AGATCT	2 cut(s)	48, 445
BisI	GCNGC	2 cut(s)	22, 25
BlsI	GCNGC	2 cut(s)	23, 26
BmeT110I	CYCGRG	1 cut(s)	483
BmsI	GCATC	1 cut(s)	360
BmtI	GCTAGC	1 cut(s)	518
BplI	GAGNNNNNCTC	2 cut(s)	416, 448
Bpu14I	TTCGAA	1 cut(s)	507
BsaJI	CCNNGG	1 cut(s)	259
BsaWI	WCCGGW	1 cut(s)	288
Bsc4I	CCNNNNNNNGG	2 cut(s)	137, 617
BseAI	TCCGGA	1 cut(s)	288
BseDI	CCNNGG	1 cut(s)	259
BseGI	GGATG	3 cut(s)	267, 322, 458
BseLI	CCNNNNNNNGG	2 cut(s)	137, 617
BseXI	GCAGC	2 cut(s)	33, 36
BseYI	CCCAGC	1 cut(s)	173
BsiHKAI	GWGCWC	2 cut(s)	242, 501
BsiHKCI	CYCGRG	1 cut(s)	483
BsiSI	CCGG	1 cut(s)	289
BslFI	GGGAC	1 cut(s)	215
BslI	CCNNNNNNNGG	2 cut(s)	137, 617
BsmAI	GTCTC	3 cut(s)	57, 288, 548
BsmFI	GGGAC	1 cut(s)	215
BsoBI	CYCGRG	1 cut(s)	483
Bsp119I	TTCGAA	1 cut(s)	507
Bsp1286I	GDGCHC	2 cut(s)	242, 501
Bsp13I	TCCGGA	1 cut(s)	288
Bsp143I	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
BspACI	CCGC	2 cut(s)	69, 618
BspEI	TCCGGA	1 cut(s)	288
BspOI	GCTAGC	1 cut(s)	518
BspPI	GGATC	2 cut(s)	280, 437
BspQI	GCTCTTC	1 cut(s)	504
BspT104I	TTCGAA	1 cut(s)	507
BssECI	CCNNGG	1 cut(s)	259
BssMI	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
BssSI	CACGAG	1 cut(s)	272
BssT1I	CCWWGG	1 cut(s)	259
Bst2BI	CACGAG	1 cut(s)	272
Bst4CI	ACNGT	4 cut(s)	169, 342, 362, 565
Bst6I	CTCTTC	2 cut(s)	417, 504
BstBI	TTCGAA	1 cut(s)	507
BstC8I	GCNNGC	1 cut(s)	516
BstF5I	GGATG	3 cut(s)	267, 322, 458
BstH2I	RGCGCY	1 cut(s)	477
BstHHI	GCGC	1 cut(s)	476
BstKTI	GATC	7 cut(s)	37, 51, 60, 124, 288, 432, 448
BstMAI	GTCTC	3 cut(s)	57, 288, 548
BstMBI	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
BstSFI	CTRYAG	2 cut(s)	168, 247
BstV1I	GCAGC	2 cut(s)	33, 36
BstX2I	RGATCY	3 cut(s)	48, 429, 445
BstYI	RGATCY	3 cut(s)	48, 429, 445
BtsCI	GGATG	3 cut(s)	267, 322, 458
BtsI	GCAGTG	1 cut(s)	499
BtsIMutI	CAGTG	3 cut(s)	165, 499, 570
Cac8I	GCNNGC	1 cut(s)	516
CfoI	GCGC	1 cut(s)	476
CviAII	CATG	1 cut(s)	356
CviJI	RGCY	8 cut(s)	99, 160, 173, 209, 240, 326, 410, 514
CviKI_1	RGCY	8 cut(s)	99, 160, 173, 209, 240, 326, 410, 514
DpnI	GATC	7 cut(s)	36, 50, 59, 123, 287, 431, 447
DpnII	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
DraI	TTTAAA	1 cut(s)	151
DrdI	GACNNNNNNGTC	1 cut(s)	71
DseDI	GACNNNNNNGTC	1 cut(s)	71
Eam1104I	CTCTTC	2 cut(s)	417, 504
EarI	CTCTTC	2 cut(s)	417, 504
EciI	GGCGGA	1 cut(s)	58
Ecl136II	GAGCTC	1 cut(s)	240
Eco130I	CCWWGG	1 cut(s)	259
Eco24I	GRGCYC	1 cut(s)	242
Eco47III	AGCGCT	1 cut(s)	475
Eco53kI	GAGCTC	1 cut(s)	240
Eco88I	CYCGRG	1 cut(s)	483
EcoICRI	GAGCTC	1 cut(s)	240
EcoT14I	CCWWGG	1 cut(s)	259
EcoT38I	GRGCYC	1 cut(s)	242
ErhI	CCWWGG	1 cut(s)	259
FaeI	CATG	1 cut(s)	359
FaiI	YATR	2 cut(s)	279, 357
FaqI	GGGAC	1 cut(s)	215
FatI	CATG	1 cut(s)	355
Fnu4HI	GCNGC	2 cut(s)	22, 25
FokI	GGATG	3 cut(s)	254, 329, 465
FriOI	GRGCYC	1 cut(s)	242
Fsp4HI	GCNGC	2 cut(s)	22, 25
FspBI	CTAG	3 cut(s)	515, 540, 615
GlaI	GCGC	1 cut(s)	475
GluI	GCNGC	2 cut(s)	22, 25
GsaI	CCCAGC	1 cut(s)	177
HaeII	RGCGCY	1 cut(s)	477
HapII	CCGG	1 cut(s)	289
HhaI	GCGC	1 cut(s)	476
Hin1II	CATG	1 cut(s)	359
Hin6I	GCGC	1 cut(s)	474
HinP1I	GCGC	1 cut(s)	474
HincII	GTYRAC	2 cut(s)	118, 234
HindII	GTYRAC	2 cut(s)	118, 234
HindIII	AAGCTT	1 cut(s)	158
HinfI	GANTC	3 cut(s)	65, 264, 398
HpaI	GTTAAC	1 cut(s)	234
HpaII	CCGG	1 cut(s)	289
Hpy166II	GTNNAC	2 cut(s)	118, 234
Hpy188I	TCNGA	4 cut(s)	62, 227, 316, 403
Hpy188III	TCNNGA	6 cut(s)	46, 187, 289, 367, 464, 485
Hpy8I	GTNNAC	2 cut(s)	118, 234
Hpy99I	CGWCG	2 cut(s)	77, 352
HpyAV	CCTTC	1 cut(s)	548
HpyCH4III	ACNGT	4 cut(s)	169, 342, 362, 565
HpyCH4IV	ACGT	2 cut(s)	230, 534
HpyCH4V	TGCA	2 cut(s)	337, 440
HpySE526I	ACGT	2 cut(s)	230, 534
Hsp92II	CATG	1 cut(s)	359
HspAI	GCGC	1 cut(s)	474
Kpn2I	TCCGGA	1 cut(s)	288
KspAI	GTTAAC	1 cut(s)	234
Kzo9I	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
LguI	GCTCTTC	1 cut(s)	504
LmnI	GCTCC	1 cut(s)	496
LpnPI	CCDG	5 cut(s)	159, 195, 200, 302, 307
Lsp1109I	GCAGC	2 cut(s)	33, 36
LweI	GCATC	1 cut(s)	360
MaeI	CTAG	3 cut(s)	515, 540, 615
MaeII	ACGT	2 cut(s)	230, 534
MalI	GATC	7 cut(s)	36, 50, 59, 123, 287, 431, 447
MboI	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
MboII	GAAGA	5 cut(s)	190, 434, 521, 535, 563
MflI	RGATCY	3 cut(s)	48, 429, 445
MhlI	GDGCHC	2 cut(s)	242, 501
MluCI	AATT	1 cut(s)	195
MlyI	GAGTC	1 cut(s)	59
MmeI	TCCRAC	1 cut(s)	132
MnlI	CCTC	4 cut(s)	213, 281, 325, 418
MroI	TCCGGA	1 cut(s)	288
MseI	TTAA	2 cut(s)	150, 233
MslI	CAYNNNNRTG	2 cut(s)	272, 360
MspA1I	CMGCKG	1 cut(s)	209
MspI	CCGG	1 cut(s)	289
NdeII	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
NheI	GCTAGC	1 cut(s)	514
NlaIII	CATG	1 cut(s)	359
NspV	TTCGAA	1 cut(s)	507
PaeR7I	CTCGAG	1 cut(s)	483
PciSI	GCTCTTC	1 cut(s)	504
PfeI	GAWTC	2 cut(s)	264, 398
PflFI	GACNNNGTC	1 cut(s)	362
PkrI	GCNGC	2 cut(s)	23, 26
PleI	GAGTC	1 cut(s)	59
PpsI	GAGTC	1 cut(s)	59
Psp124BI	GAGCTC	1 cut(s)	242
Psp1406I	AACGTT	1 cut(s)	230
PspFI	CCCAGC	1 cut(s)	173
PsuI	RGATCY	3 cut(s)	48, 429, 445
PsyI	GACNNNGTC	1 cut(s)	362
PvuII	CAGCTG	1 cut(s)	209
RseI	CAYNNNNRTG	2 cut(s)	272, 360
SacI	GAGCTC	1 cut(s)	242
SapI	GCTCTTC	1 cut(s)	504
SaqAI	TTAA	2 cut(s)	150, 233
SatI	GCNGC	2 cut(s)	22, 25
Sau3AI	GATC	7 cut(s)	34, 48, 57, 121, 285, 429, 445
SchI	GAGTC	1 cut(s)	59
SduI	GDGCHC	2 cut(s)	242, 501
SfaNI	GCATC	1 cut(s)	360
SfcI	CTRYAG	2 cut(s)	168, 247
Sfr274I	CTCGAG	1 cut(s)	483
SfuI	TTCGAA	1 cut(s)	507
SlaI	CTCGAG	1 cut(s)	483
SmiMI	CAYNNNNRTG	2 cut(s)	272, 360
SmlI	CTYRAG	1 cut(s)	483
SmoI	CTYRAG	1 cut(s)	483
Sse9I	AATT	1 cut(s)	195
SsiI	CCGC	2 cut(s)	69, 618
SspI	AATATT	1 cut(s)	580
SspMI	CTAG	3 cut(s)	515, 540, 615
SstI	GAGCTC	1 cut(s)	242
StyI	CCWWGG	1 cut(s)	259
TaaI	ACNGT	4 cut(s)	169, 342, 362, 565
TaiI	ACGT	2 cut(s)	233, 537
TaqI	TCGA	3 cut(s)	347, 484, 507
TasI	AATT	1 cut(s)	195
TfiI	GAWTC	2 cut(s)	264, 398
Tru1I	TTAA	2 cut(s)	150, 233
Tru9I	TTAA	2 cut(s)	150, 233
TscAI	CASTG	3 cut(s)	172, 506, 570
TseI	GCWGC	2 cut(s)	21, 24
TspDTI	ATGAA	1 cut(s)	91
TspRI	CASTG	3 cut(s)	172, 506, 570
Tth111I	GACNNNGTC	1 cut(s)	362
XhoI	CTCGAG	1 cut(s)	483
XspI	CTAG	3 cut(s)	515, 540, 615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.