Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003572

Physical Location & Seq

Genomic Coordinates Forward (+)

19618986 .. 19621568

Gene Structure 2583 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1111400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 711 bp

ATGGCGTACAAGCATGAGGAACTTCCTCCACCGTTGGATTCCACTTCCTCCCCTCCTCCTCTCTTTGACGGCACTACAAGGCTGTACGTAAATTACACATGTCCATTTGTACAGCGGGTTTGGATCACTAGGAACTATAAGGGTTTGCAAGACAAGATTAAGTTAGTGGGTATAGATCTTGAAAACAGGCCTGCTTGGTACAAGGAAAAAGTCTACCCCGGAAACAAGGTACCATCTCTGGAGCACAATGGAAAAGTCATTGGAGAAAGTCTTGATCTGATCAAATACCTCGAAAGCAACTTTGAGGGGCCTTCACTTGTCCCCAGTGATGACCCTGAGAGAAAAAAGTTTGGCGAAGAATTATTAACTTATGTTGATACCTTCGTGGGGTCCTTGTATCGCTCATTGAAAGGAGACACGGTAAAAGCAGCTGATGAGCAGTTTGATTACTTGGAAAATGCTCTTAAGAAATTTGACGATGGGCCATATCTTCTGGGTCAGTTCAGTTTGGTGGACATAGCCTATATCCCATTCGTTGAGAAGTTCCAAGTCTTCTTATCAGACGTGTTCAAGTATGACATCACAGCTGGGAGGCCTAAACTAGCAGCATTCTTGGAGGAAATCAACAAGATTGAGGCATACAAGGTAACCAAAGCTGATCCAAAGGAGATTGTTGCAGAATACAAGAAGAAATTCCTGGAGCAACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0010731 GO:0016740 GO:0016765 GO:0019538 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C_2 GST_N_3

Protein Analysis

237

Amino Acids

27.11

Weight (kDa)

5.3

Isoelectric Point (pI)

33.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_3	PF13417	28 - 104	5.4e-22	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	33 - 99	3.8e-18	Glutathione S-transferase, N-terminal domain
GST_C_5	PF16865	119 - 206	4.6e-08	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	142 - 206	3e-08	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02780 AT5G02780 AT5G02780 AT5G02790
fragaria_vesca	FvH4_6g14970 FvH4_6g14970
malus_domestica	MD04G1111400.v1.1 MD04G1111600.v1.1 MD04G1111700.v1.1 MD04G1112000.v1.1 MD04G1112100.v1.1 MD12G1129400.v1.1
prunus_persica	Prupe.6G244700_v2.0.a1
pyrus_communis	pycom04g10330 pycom04g10340 pycom12g12570
rosa_chinensis	RchiOBHm_Chr3g0468151 RchiOBHm_Chr3g0468161
rosa_laevigata	RLG00000024444
rosa_multiflora	Rmu_sc0001810.1_g000001 Rmu_sc0003659.1_g000008 Rmu_sc0005590.1_g000002 Rmu_sc0021707.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412980
rosa_rugosa	Rorug03G0093700 Rorug03G0093700
rosa_samantha	Rh3AG141600 Rh3AG141700 Rh3BG163100 Rh3CG163000 Rh3CG163200 Rh3DG163600 Rh3DG163800
rosa_wichuraiana	Rw3G013380 Rw3G013390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	229
AccB1I	GGYRCC	1 cut(s)	229
AccI	GTMKAC	1 cut(s)	213
AciI	CCGC	1 cut(s)	115
AclWI	GGATC	2 cut(s)	131, 653
AcsI	RAATTY	2 cut(s)	470, 692
AfaI	GTAC	5 cut(s)	8, 86, 111, 200, 231
AfiI	CCNNNNNNNGG	1 cut(s)	387
AflII	CTTAAG	1 cut(s)	464
AflIII	ACRYGT	2 cut(s)	98, 564
AgsI	TTSAA	3 cut(s)	182, 409, 571
AjiI	CACGTC	1 cut(s)	565
AjnI	CCWGG	1 cut(s)	696
AluBI	AGCT	3 cut(s)	431, 587, 656
AluI	AGCT	3 cut(s)	431, 587, 656
Alw21I	GWGCWC	1 cut(s)	246
Alw26I	GTCTC	1 cut(s)	408
AlwI	GGATC	2 cut(s)	131, 653
AoxI	GGCC	4 cut(s)	188, 308, 482, 593
ApeKI	GCWGC	2 cut(s)	428, 605
ApoI	RAATTY	2 cut(s)	470, 692
Asp700I	GAANNNNTTC	1 cut(s)	692
Asp718I	GGTACC	1 cut(s)	229
AspS9I	GGNCC	3 cut(s)	308, 390, 482
AsuC2I	CCSGG	1 cut(s)	219
AvaII	GGWCC	1 cut(s)	390
BaeI	ACNNNNGTAYC	2 cut(s)	369, 402
BanI	GGYRCC	1 cut(s)	229
BbsI	GAAGAC	1 cut(s)	544
Bbv12I	GWGCWC	1 cut(s)	246
BbvI	GCAGC	2 cut(s)	440, 617
BccI	CCATC	2 cut(s)	241, 473
BceAI	ACGGC	1 cut(s)	85
BciT130I	CCWGG	1 cut(s)	698
BclI	TGATCA	1 cut(s)	279
BcnI	CCSGG	1 cut(s)	219
BcoDI	GTCTC	1 cut(s)	408
BfaI	CTAG	2 cut(s)	129, 602
BfrI	CTTAAG	1 cut(s)	464
BglII	AGATCT	1 cut(s)	175
BisI	GCNGC	2 cut(s)	429, 606
BlsI	GCNGC	2 cut(s)	430, 607
Bme1390I	CCNGG	2 cut(s)	219, 698
Bme18I	GGWCC	1 cut(s)	390
BmgBI	CACGTC	1 cut(s)	565
BmgT120I	GGNCC	3 cut(s)	308, 390, 482
BmiI	GGNNCC	3 cut(s)	231, 309, 391
BmrFI	CCNGG	2 cut(s)	219, 698
BmrI	ACTGGG	1 cut(s)	318
BmuI	ACTGGG	1 cut(s)	318
BpiI	GAAGAC	1 cut(s)	544
BpmI	CTGGAG	1 cut(s)	260
BpuMI	CCSGG	1 cut(s)	219
BsaAI	YACGTR	1 cut(s)	88
BsaJI	CCNNGG	1 cut(s)	217
BsaXI	ACNNNNNCTCC	3 cut(s)	405, 435, 692
Bsc4I	CCNNNNNNNGG	1 cut(s)	387
Bse1I	ACTGG	1 cut(s)	324
BseBI	CCWGG	1 cut(s)	698
BseDI	CCNNGG	1 cut(s)	217
BseLI	CCNNNNNNNGG	1 cut(s)	387
BseMII	CTCAG	1 cut(s)	327
BseNI	ACTGG	1 cut(s)	324
BseRI	GAGGAG	2 cut(s)	45, 48
BseXI	GCAGC	2 cut(s)	440, 617
BseYI	CCCAGC	1 cut(s)	587
BshFI	GGCC	4 cut(s)	190, 310, 484, 595
BshNI	GGYRCC	1 cut(s)	229
BsiHKAI	GWGCWC	1 cut(s)	246
BsiSI	CCGG	1 cut(s)	219
BslFI	GGGAC	1 cut(s)	305
BslI	CCNNNNNNNGG	1 cut(s)	387
BsmAI	GTCTC	1 cut(s)	408
BsmFI	GGGAC	1 cut(s)	305
BsmI	GAATGC	1 cut(s)	608
BsnI	GGCC	4 cut(s)	190, 310, 484, 595
Bsp1286I	GDGCHC	1 cut(s)	246
Bsp1407I	TGTACA	1 cut(s)	109
Bsp143I	GATC	5 cut(s)	123, 175, 274, 279, 658
BspACI	CCGC	1 cut(s)	115
BspANI	GGCC	4 cut(s)	190, 310, 484, 595
BspCNI	CTCAG	1 cut(s)	328
BspLI	GGNNCC	3 cut(s)	231, 309, 391
BspPI	GGATC	2 cut(s)	131, 653
BspT107I	GGYRCC	1 cut(s)	229
BspTI	CTTAAG	1 cut(s)	464
BsrGI	TGTACA	1 cut(s)	109
BsrI	ACTGG	1 cut(s)	324
BssECI	CCNNGG	1 cut(s)	217
BssMI	GATC	5 cut(s)	123, 175, 274, 279, 658
Bst2UI	CCWGG	1 cut(s)	698
Bst4CI	ACNGT	3 cut(s)	33, 421, 708
BstAFI	CTTAAG	1 cut(s)	464
BstAUI	TGTACA	1 cut(s)	109
BstBAI	YACGTR	1 cut(s)	88
BstC8I	GCNNGC	1 cut(s)	192
BstDEI	CTNAG	1 cut(s)	336
BstEII	GGTNACC	1 cut(s)	646
BstKTI	GATC	5 cut(s)	126, 178, 277, 282, 661
BstMAI	GTCTC	1 cut(s)	408
BstMBI	GATC	5 cut(s)	123, 175, 274, 279, 658
BstNI	CCWGG	1 cut(s)	698
BstNSI	RCATGY	1 cut(s)	102
BstPI	GGTNACC	1 cut(s)	646
BstSCI	CCNGG	2 cut(s)	217, 696
BstSNI	TACGTA	1 cut(s)	88
BstV1I	GCAGC	2 cut(s)	440, 617
BstV2I	GAAGAC	1 cut(s)	544
BstX2I	RGATCY	1 cut(s)	175
BstYI	RGATCY	1 cut(s)	175
BsuRI	GGCC	4 cut(s)	190, 310, 484, 595
BtrI	CACGTC	1 cut(s)	565
BtsIMutI	CAGTG	1 cut(s)	331
Cac8I	GCNNGC	1 cut(s)	192
Cfr13I	GGNCC	3 cut(s)	308, 390, 482
Csp6I	GTAC	5 cut(s)	7, 85, 110, 199, 230
CviAII	CATG	2 cut(s)	14, 99
CviJI	RGCY	9 cut(s)	82, 190, 310, 431, 484, 521, 587, 595, 656
CviKI_1	RGCY	9 cut(s)	82, 190, 310, 431, 484, 521, 587, 595, 656
CviQI	GTAC	5 cut(s)	7, 85, 110, 199, 230
DdeI	CTNAG	1 cut(s)	336
DpnI	GATC	5 cut(s)	125, 177, 276, 281, 660
DpnII	GATC	5 cut(s)	123, 175, 274, 279, 658
Eco105I	TACGTA	1 cut(s)	88
Eco147I	AGGCCT	2 cut(s)	190, 595
Eco47I	GGWCC	1 cut(s)	390
Eco91I	GGTNACC	1 cut(s)	646
EcoO109I	RGGNCCY	2 cut(s)	308, 390
EcoO65I	GGTNACC	1 cut(s)	646
EcoRII	CCWGG	1 cut(s)	696
FaeI	CATG	2 cut(s)	17, 102
FaqI	GGGAC	1 cut(s)	305
FatI	CATG	2 cut(s)	13, 98
FauI	CCCGC	1 cut(s)	108
FbaI	TGATCA	1 cut(s)	279
FblI	GTMKAC	1 cut(s)	213
Fnu4HI	GCNGC	2 cut(s)	429, 606
Fsp4HI	GCNGC	2 cut(s)	429, 606
FspBI	CTAG	2 cut(s)	129, 602
GluI	GCNGC	2 cut(s)	429, 606
GsaI	CCCAGC	1 cut(s)	591
GsuI	CTGGAG	1 cut(s)	260
HaeIII	GGCC	4 cut(s)	190, 310, 484, 595
HapII	CCGG	1 cut(s)	219
Hin1II	CATG	2 cut(s)	17, 102
HinfI	GANTC	1 cut(s)	38
HpaII	CCGG	1 cut(s)	219
Hpy166II	GTNNAC	2 cut(s)	214, 514
Hpy188I	TCNGA	2 cut(s)	279, 562
Hpy188III	TCNNGA	3 cut(s)	179, 239, 272
Hpy8I	GTNNAC	2 cut(s)	214, 514
HpyAV	CCTTC	2 cut(s)	321, 391
HpyCH4III	ACNGT	3 cut(s)	33, 421, 708
HpyCH4IV	ACGT	2 cut(s)	87, 564
HpyCH4V	TGCA	2 cut(s)	148, 677
HpyF3I	CTNAG	1 cut(s)	336
HpySE526I	ACGT	2 cut(s)	87, 564
Hsp92II	CATG	2 cut(s)	17, 102
KpnI	GGTACC	1 cut(s)	233
Ksp22I	TGATCA	1 cut(s)	279
Kzo9I	GATC	5 cut(s)	123, 175, 274, 279, 658
LmnI	GCTCC	2 cut(s)	241, 700
LpnPI	CCDG	9 cut(s)	172, 204, 224, 232, 337, 348, 479, 573, 683
Lsp1109I	GCAGC	2 cut(s)	440, 617
MaeI	CTAG	2 cut(s)	129, 602
MaeII	ACGT	2 cut(s)	87, 564
MaeIII	GTNAC	1 cut(s)	646
MalI	GATC	5 cut(s)	125, 177, 276, 281, 660
MboI	GATC	5 cut(s)	123, 175, 274, 279, 658
MboII	GAAGA	4 cut(s)	368, 482, 544, 700
MflI	RGATCY	1 cut(s)	175
MhlI	GDGCHC	1 cut(s)	246
MluCI	AATT	4 cut(s)	91, 359, 470, 692
MmeI	TCCRAC	1 cut(s)	15
MroXI	GAANNNNTTC	1 cut(s)	692
MseI	TTAA	3 cut(s)	159, 365, 465
MspA1I	CMGCKG	3 cut(s)	115, 431, 587
MspCI	CTTAAG	1 cut(s)	464
MspI	CCGG	1 cut(s)	219
MspR9I	CCNGG	2 cut(s)	219, 698
Mva1269I	GAATGC	1 cut(s)	608
MvaI	CCWGG	1 cut(s)	698
NciI	CCSGG	1 cut(s)	219
NdeII	GATC	5 cut(s)	123, 175, 274, 279, 658
NlaIII	CATG	2 cut(s)	17, 102
NlaIV	GGNNCC	3 cut(s)	231, 309, 391
NspI	RCATGY	1 cut(s)	102
PceI	AGGCCT	2 cut(s)	190, 595
PciI	ACATGT	1 cut(s)	98
PctI	GAATGC	1 cut(s)	608
PdmI	GAANNNNTTC	1 cut(s)	692
PfeI	GAWTC	1 cut(s)	38
PfoI	TCCNGGA	1 cut(s)	696
PkrI	GCNGC	2 cut(s)	430, 607
Ppu21I	YACGTR	1 cut(s)	88
PpuMI	RGGWCCY	1 cut(s)	390
PscI	ACATGT	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	390
Psp6I	CCWGG	1 cut(s)	696
PspEI	GGTNACC	1 cut(s)	646
PspFI	CCCAGC	1 cut(s)	587
PspGI	CCWGG	1 cut(s)	696
PspN4I	GGNNCC	3 cut(s)	231, 309, 391
PspPI	GGNCC	3 cut(s)	308, 390, 482
PspPPI	RGGWCCY	1 cut(s)	390
PsuI	RGATCY	1 cut(s)	175
PvuII	CAGCTG	2 cut(s)	431, 587
RsaI	GTAC	5 cut(s)	8, 86, 111, 200, 231
RsaNI	GTAC	5 cut(s)	7, 85, 110, 199, 230
SaqAI	TTAA	3 cut(s)	159, 365, 465
SatI	GCNGC	2 cut(s)	429, 606
Sau3AI	GATC	5 cut(s)	123, 175, 274, 279, 658
Sau96I	GGNCC	3 cut(s)	308, 390, 482
ScrFI	CCNGG	2 cut(s)	219, 698
SduI	GDGCHC	1 cut(s)	246
SetI	ASST	9 cut(s)	90, 231, 291, 383, 433, 567, 589, 648, 658
SinI	GGWCC	1 cut(s)	390
SmlI	CTYRAG	1 cut(s)	464
SmoI	CTYRAG	1 cut(s)	464
SnaBI	TACGTA	1 cut(s)	88
Sse9I	AATT	4 cut(s)	91, 359, 470, 692
SseBI	AGGCCT	2 cut(s)	190, 595
SsiI	CCGC	1 cut(s)	115
SspMI	CTAG	2 cut(s)	129, 602
StuI	AGGCCT	2 cut(s)	190, 595
StyD4I	CCNGG	2 cut(s)	217, 696
TaaI	ACNGT	3 cut(s)	33, 421, 708
TaiI	ACGT	2 cut(s)	90, 567
TaqI	TCGA	1 cut(s)	291
TasI	AATT	4 cut(s)	91, 359, 470, 692
TatI	WGTACW	1 cut(s)	109
TfiI	GAWTC	1 cut(s)	38
Tru1I	TTAA	3 cut(s)	159, 365, 465
Tru9I	TTAA	3 cut(s)	159, 365, 465
TscAI	CASTG	1 cut(s)	331
TseI	GCWGC	2 cut(s)	428, 605
TspRI	CASTG	1 cut(s)	331
Vha464I	CTTAAG	1 cut(s)	464
VpaK11BI	GGWCC	1 cut(s)	390
XapI	RAATTY	2 cut(s)	470, 692
XceI	RCATGY	1 cut(s)	102
XmiI	GTMKAC	1 cut(s)	213
XmnI	GAANNNNTTC	1 cut(s)	692
XspI	CTAG	2 cut(s)	129, 602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.