Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003572

Physical Location & Seq

Genomic Coordinates Forward (+)

19786012 .. 19788623

Gene Structure 2612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1112000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 735 bp

ATGGCGTACAAGCATGAGGAACTTCCTCCACCGTTGGATTCCACTTCCTCCCCTCCTCCTCTCTTTGACGGCACTACAAGGCTGTACGTAAATTACACATGTCCATTTGTACAGCGGGTTTGGATCACTAGGAACTATAAGGGTTTGCAAGACAAGATTAAGTTAGTGGGTATAGATCTTGAAAACAGGCCTGCTTGGTACAAGGAAAAAGTCTACCCCCGGAAACAAGGTAAAATTTTGAGCAAAAAATTAGTACCATCTCTGGAGCACAATGGAAAAGTCATTGGAGAAAGTCTTGATCTGATCAAATACCTCGAAAGCAACTTTGAGGGGCCTTCACTTGTCCCCAGTGATGACCCTGAGAGAAAAAAGTTTGGCGAAGAATTATTAACTTATGTTGATACCTTCGTGGGGTCCTTGTATCGCTCATTGAAAGGAGACACGGTAAAAGCAGCTGATGAGCAGTTTGATTACTTGGAAAATGCTCTTAAGAAATTTGACGATGGGCCATATCTTCTGGGTCAGTTCAGTTTGGTGGACATAGCCTATATCCCATTCGTTGAGAAGTTCCAAGTCTTCTTATCAGACGTGTTCAAGTATGACATCACAGCTGGGAGGCCTAAACTAGCAGCATTCTTGGAGGAAATCAACAAGATTGAGGCATACAAGGTAACCAAAGCTGATCCAAAGGAGATTGTTGCAGCGTACAAGAAGAAATTCCTGGAGCAACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0010731 GO:0016740 GO:0016765 GO:0019538 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C_2 GST_N_3

Protein Analysis

245

Amino Acids

28.04

Weight (kDa)

6.77

Isoelectric Point (pI)

33.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_3	PF13417	28 - 112	1.9e-17	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	33 - 107	1.6e-15	Glutathione S-transferase, N-terminal domain
GST_C_5	PF16865	127 - 214	5e-08	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	150 - 214	3.4e-08	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02780 AT5G02780 AT5G02780 AT5G02790
fragaria_vesca	FvH4_6g14970 FvH4_6g14970
malus_domestica	MD04G1111400.v1.1 MD04G1111600.v1.1 MD04G1111700.v1.1 MD04G1112000.v1.1 MD04G1112100.v1.1 MD12G1129400.v1.1
prunus_persica	Prupe.6G244700_v2.0.a1
pyrus_communis	pycom04g10330 pycom04g10340 pycom12g12570
rosa_chinensis	RchiOBHm_Chr3g0468151 RchiOBHm_Chr3g0468161
rosa_laevigata	RLG00000024444
rosa_multiflora	Rmu_sc0001810.1_g000001 Rmu_sc0003659.1_g000008 Rmu_sc0005590.1_g000002 Rmu_sc0021707.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412980
rosa_rugosa	Rorug03G0093700 Rorug03G0093700
rosa_samantha	Rh3AG141600 Rh3AG141700 Rh3BG163100 Rh3CG163000 Rh3CG163200 Rh3DG163600 Rh3DG163800
rosa_wichuraiana	Rw3G013380 Rw3G013390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	213
AciI	CCGC	1 cut(s)	115
AclWI	GGATC	2 cut(s)	131, 677
AcsI	RAATTY	3 cut(s)	234, 494, 716
AfaI	GTAC	6 cut(s)	8, 86, 111, 200, 255, 707
AfiI	CCNNNNNNNGG	1 cut(s)	411
AflII	CTTAAG	1 cut(s)	488
AflIII	ACRYGT	2 cut(s)	98, 588
AgsI	TTSAA	3 cut(s)	182, 433, 595
AjiI	CACGTC	1 cut(s)	589
AjnI	CCWGG	1 cut(s)	720
AluBI	AGCT	3 cut(s)	455, 611, 680
AluI	AGCT	3 cut(s)	455, 611, 680
Alw21I	GWGCWC	1 cut(s)	270
Alw26I	GTCTC	1 cut(s)	432
AlwI	GGATC	2 cut(s)	131, 677
AoxI	GGCC	4 cut(s)	188, 332, 506, 617
ApeKI	GCWGC	3 cut(s)	452, 629, 701
ApoI	RAATTY	3 cut(s)	234, 494, 716
Asp700I	GAANNNNTTC	1 cut(s)	716
AspS9I	GGNCC	3 cut(s)	332, 414, 506
AsuC2I	CCSGG	1 cut(s)	220
AvaII	GGWCC	1 cut(s)	414
BaeI	ACNNNNGTAYC	2 cut(s)	393, 426
BbsI	GAAGAC	1 cut(s)	568
Bbv12I	GWGCWC	1 cut(s)	270
BbvI	GCAGC	3 cut(s)	464, 641, 713
BccI	CCATC	2 cut(s)	265, 497
BceAI	ACGGC	1 cut(s)	85
BciT130I	CCWGG	1 cut(s)	722
BclI	TGATCA	1 cut(s)	303
BcnI	CCSGG	1 cut(s)	220
BcoDI	GTCTC	1 cut(s)	432
BfaI	CTAG	2 cut(s)	129, 626
BfrI	CTTAAG	1 cut(s)	488
BglII	AGATCT	1 cut(s)	175
BisI	GCNGC	3 cut(s)	453, 630, 702
BlsI	GCNGC	3 cut(s)	454, 631, 703
Bme1390I	CCNGG	2 cut(s)	220, 722
Bme18I	GGWCC	1 cut(s)	414
BmgBI	CACGTC	1 cut(s)	589
BmgT120I	GGNCC	3 cut(s)	332, 414, 506
BmiI	GGNNCC	2 cut(s)	333, 415
BmrFI	CCNGG	2 cut(s)	220, 722
BmrI	ACTGGG	1 cut(s)	342
BmuI	ACTGGG	1 cut(s)	342
BpiI	GAAGAC	1 cut(s)	568
BpmI	CTGGAG	1 cut(s)	284
BpuMI	CCSGG	1 cut(s)	220
BsaAI	YACGTR	1 cut(s)	88
BsaJI	CCNNGG	1 cut(s)	218
BsaXI	ACNNNNNCTCC	3 cut(s)	429, 459, 716
Bsc4I	CCNNNNNNNGG	1 cut(s)	411
Bse1I	ACTGG	1 cut(s)	348
BseBI	CCWGG	1 cut(s)	722
BseDI	CCNNGG	1 cut(s)	218
BseLI	CCNNNNNNNGG	1 cut(s)	411
BseMII	CTCAG	1 cut(s)	351
BseNI	ACTGG	1 cut(s)	348
BseRI	GAGGAG	2 cut(s)	45, 48
BseXI	GCAGC	3 cut(s)	464, 641, 713
BseYI	CCCAGC	1 cut(s)	611
BshFI	GGCC	4 cut(s)	190, 334, 508, 619
BsiHKAI	GWGCWC	1 cut(s)	270
BsiSI	CCGG	1 cut(s)	220
BslFI	GGGAC	1 cut(s)	329
BslI	CCNNNNNNNGG	1 cut(s)	411
BsmAI	GTCTC	1 cut(s)	432
BsmFI	GGGAC	1 cut(s)	329
BsmI	GAATGC	1 cut(s)	632
BsnI	GGCC	4 cut(s)	190, 334, 508, 619
Bsp1286I	GDGCHC	1 cut(s)	270
Bsp1407I	TGTACA	1 cut(s)	109
Bsp143I	GATC	5 cut(s)	123, 175, 298, 303, 682
BspACI	CCGC	1 cut(s)	115
BspANI	GGCC	4 cut(s)	190, 334, 508, 619
BspCNI	CTCAG	1 cut(s)	352
BspLI	GGNNCC	2 cut(s)	333, 415
BspPI	GGATC	2 cut(s)	131, 677
BspTI	CTTAAG	1 cut(s)	488
BsrGI	TGTACA	1 cut(s)	109
BsrI	ACTGG	1 cut(s)	348
BssECI	CCNNGG	1 cut(s)	218
BssMI	GATC	5 cut(s)	123, 175, 298, 303, 682
Bst2UI	CCWGG	1 cut(s)	722
Bst4CI	ACNGT	3 cut(s)	33, 445, 732
BstAFI	CTTAAG	1 cut(s)	488
BstAUI	TGTACA	1 cut(s)	109
BstBAI	YACGTR	1 cut(s)	88
BstC8I	GCNNGC	1 cut(s)	192
BstDEI	CTNAG	1 cut(s)	360
BstEII	GGTNACC	1 cut(s)	670
BstKTI	GATC	5 cut(s)	126, 178, 301, 306, 685
BstMAI	GTCTC	1 cut(s)	432
BstMBI	GATC	5 cut(s)	123, 175, 298, 303, 682
BstNI	CCWGG	1 cut(s)	722
BstNSI	RCATGY	1 cut(s)	102
BstPI	GGTNACC	1 cut(s)	670
BstSCI	CCNGG	2 cut(s)	218, 720
BstSNI	TACGTA	1 cut(s)	88
BstV1I	GCAGC	3 cut(s)	464, 641, 713
BstV2I	GAAGAC	1 cut(s)	568
BstX2I	RGATCY	1 cut(s)	175
BstYI	RGATCY	1 cut(s)	175
BsuRI	GGCC	4 cut(s)	190, 334, 508, 619
BtrI	CACGTC	1 cut(s)	589
BtsIMutI	CAGTG	1 cut(s)	355
Cac8I	GCNNGC	1 cut(s)	192
Cfr13I	GGNCC	3 cut(s)	332, 414, 506
Csp6I	GTAC	6 cut(s)	7, 85, 110, 199, 254, 706
CviAII	CATG	2 cut(s)	14, 99
CviJI	RGCY	9 cut(s)	82, 190, 334, 455, 508, 545, 611, 619, 680
CviKI_1	RGCY	9 cut(s)	82, 190, 334, 455, 508, 545, 611, 619, 680
CviQI	GTAC	6 cut(s)	7, 85, 110, 199, 254, 706
DdeI	CTNAG	1 cut(s)	360
DpnI	GATC	5 cut(s)	125, 177, 300, 305, 684
DpnII	GATC	5 cut(s)	123, 175, 298, 303, 682
Eco105I	TACGTA	1 cut(s)	88
Eco147I	AGGCCT	2 cut(s)	190, 619
Eco47I	GGWCC	1 cut(s)	414
Eco91I	GGTNACC	1 cut(s)	670
EcoO109I	RGGNCCY	2 cut(s)	332, 414
EcoO65I	GGTNACC	1 cut(s)	670
EcoRII	CCWGG	1 cut(s)	720
FaeI	CATG	2 cut(s)	17, 102
FaqI	GGGAC	1 cut(s)	329
FatI	CATG	2 cut(s)	13, 98
FauI	CCCGC	1 cut(s)	108
FbaI	TGATCA	1 cut(s)	303
FblI	GTMKAC	1 cut(s)	213
Fnu4HI	GCNGC	3 cut(s)	453, 630, 702
Fsp4HI	GCNGC	3 cut(s)	453, 630, 702
FspBI	CTAG	2 cut(s)	129, 626
GluI	GCNGC	3 cut(s)	453, 630, 702
GsaI	CCCAGC	1 cut(s)	615
GsuI	CTGGAG	1 cut(s)	284
HaeIII	GGCC	4 cut(s)	190, 334, 508, 619
HapII	CCGG	1 cut(s)	220
Hin1II	CATG	2 cut(s)	17, 102
HinfI	GANTC	1 cut(s)	38
HpaII	CCGG	1 cut(s)	220
Hpy166II	GTNNAC	2 cut(s)	214, 538
Hpy188I	TCNGA	2 cut(s)	303, 586
Hpy188III	TCNNGA	3 cut(s)	179, 263, 296
Hpy8I	GTNNAC	2 cut(s)	214, 538
HpyAV	CCTTC	2 cut(s)	345, 415
HpyCH4III	ACNGT	3 cut(s)	33, 445, 732
HpyCH4IV	ACGT	2 cut(s)	87, 588
HpyCH4V	TGCA	2 cut(s)	148, 701
HpyF3I	CTNAG	1 cut(s)	360
HpySE526I	ACGT	2 cut(s)	87, 588
Hsp92II	CATG	2 cut(s)	17, 102
Ksp22I	TGATCA	1 cut(s)	303
Kzo9I	GATC	5 cut(s)	123, 175, 298, 303, 682
LmnI	GCTCC	2 cut(s)	265, 724
LpnPI	CCDG	9 cut(s)	172, 204, 233, 248, 361, 372, 503, 597, 707
Lsp1109I	GCAGC	3 cut(s)	464, 641, 713
MaeI	CTAG	2 cut(s)	129, 626
MaeII	ACGT	2 cut(s)	87, 588
MaeIII	GTNAC	1 cut(s)	670
MalI	GATC	5 cut(s)	125, 177, 300, 305, 684
MboI	GATC	5 cut(s)	123, 175, 298, 303, 682
MboII	GAAGA	4 cut(s)	392, 506, 568, 724
MflI	RGATCY	1 cut(s)	175
MhlI	GDGCHC	1 cut(s)	270
MluCI	AATT	6 cut(s)	91, 234, 248, 383, 494, 716
MmeI	TCCRAC	1 cut(s)	15
MroXI	GAANNNNTTC	1 cut(s)	716
MseI	TTAA	3 cut(s)	159, 389, 489
MspA1I	CMGCKG	3 cut(s)	115, 455, 611
MspCI	CTTAAG	1 cut(s)	488
MspI	CCGG	1 cut(s)	220
MspR9I	CCNGG	2 cut(s)	220, 722
Mva1269I	GAATGC	1 cut(s)	632
MvaI	CCWGG	1 cut(s)	722
NciI	CCSGG	1 cut(s)	220
NdeII	GATC	5 cut(s)	123, 175, 298, 303, 682
NlaIII	CATG	2 cut(s)	17, 102
NlaIV	GGNNCC	2 cut(s)	333, 415
NspI	RCATGY	1 cut(s)	102
PceI	AGGCCT	2 cut(s)	190, 619
PciI	ACATGT	1 cut(s)	98
PctI	GAATGC	1 cut(s)	632
PdmI	GAANNNNTTC	1 cut(s)	716
PfeI	GAWTC	1 cut(s)	38
PfoI	TCCNGGA	1 cut(s)	720
PkrI	GCNGC	3 cut(s)	454, 631, 703
Ppu21I	YACGTR	1 cut(s)	88
PpuMI	RGGWCCY	1 cut(s)	414
PscI	ACATGT	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	414
Psp6I	CCWGG	1 cut(s)	720
PspEI	GGTNACC	1 cut(s)	670
PspFI	CCCAGC	1 cut(s)	611
PspGI	CCWGG	1 cut(s)	720
PspN4I	GGNNCC	2 cut(s)	333, 415
PspPI	GGNCC	3 cut(s)	332, 414, 506
PspPPI	RGGWCCY	1 cut(s)	414
PsuI	RGATCY	1 cut(s)	175
PvuII	CAGCTG	2 cut(s)	455, 611
RsaI	GTAC	6 cut(s)	8, 86, 111, 200, 255, 707
RsaNI	GTAC	6 cut(s)	7, 85, 110, 199, 254, 706
SaqAI	TTAA	3 cut(s)	159, 389, 489
SatI	GCNGC	3 cut(s)	453, 630, 702
Sau3AI	GATC	5 cut(s)	123, 175, 298, 303, 682
Sau96I	GGNCC	3 cut(s)	332, 414, 506
ScrFI	CCNGG	2 cut(s)	220, 722
SduI	GDGCHC	1 cut(s)	270
SetI	ASST	9 cut(s)	90, 232, 315, 407, 457, 591, 613, 672, 682
SinI	GGWCC	1 cut(s)	414
SmlI	CTYRAG	1 cut(s)	488
SmoI	CTYRAG	1 cut(s)	488
SnaBI	TACGTA	1 cut(s)	88
Sse9I	AATT	6 cut(s)	91, 234, 248, 383, 494, 716
SseBI	AGGCCT	2 cut(s)	190, 619
SsiI	CCGC	1 cut(s)	115
SspMI	CTAG	2 cut(s)	129, 626
StuI	AGGCCT	2 cut(s)	190, 619
StyD4I	CCNGG	2 cut(s)	218, 720
TaaI	ACNGT	3 cut(s)	33, 445, 732
TaiI	ACGT	2 cut(s)	90, 591
TaqI	TCGA	1 cut(s)	315
TasI	AATT	6 cut(s)	91, 234, 248, 383, 494, 716
TatI	WGTACW	1 cut(s)	109
TfiI	GAWTC	1 cut(s)	38
Tru1I	TTAA	3 cut(s)	159, 389, 489
Tru9I	TTAA	3 cut(s)	159, 389, 489
TscAI	CASTG	1 cut(s)	355
TseI	GCWGC	3 cut(s)	452, 629, 701
TspRI	CASTG	1 cut(s)	355
Vha464I	CTTAAG	1 cut(s)	488
VpaK11BI	GGWCC	1 cut(s)	414
XapI	RAATTY	3 cut(s)	234, 494, 716
XceI	RCATGY	1 cut(s)	102
XmiI	GTMKAC	1 cut(s)	213
XmnI	GAANNNNTTC	1 cut(s)	716
XspI	CTAG	2 cut(s)	129, 626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.