Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003572

Physical Location & Seq

Genomic Coordinates Forward (+)

19745395 .. 19747807

Gene Structure 2413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1111700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 678 bp

ATGGCGTACAAGCATGAGGAACTTCCTCCACCGTTGGATTCCACTTCCTCCCCTCCTCCTCTCTTTGACGGCACTACAAGGCTGTACGTAAATTACACAAGTCCATTTGCACAGCGGGTTTGGATCACTAGGAACTATAAGATCTTGAAAACAGGCCCTGCTTGGTACAAGGAAAAAGTCTACCCCGGAAACAAGGTGCCATCTCTGGAGCACAATGGAAAAGTCATTGGAGAAAGTCTTGATCTGATCAAATACCTCGAAAGCAACTTCGAAGGGCCTTCACTTGTCCCCAATGACGACCCTGAGAGAAAAAAGTTTGGCCAAGAATTATTAAATTATGTTGATACCTTTGTGGGGTCCTTGTATCGCTCATTGAAAGGAGACACGGTAAAAGCAGCTGATGAGCAGTTTGATTACTTGGAAAATGCTCTTAAGAAATTTGACGATGGGCCATATCTTCTGGGTCAGTTCAGTTTGGTGGACATAGCCTATATCCCGTTCGTTGAGCAGTTCCAAGTCTTCTTATCAGACGTGTTCAAGTATGACATCACAGCTGGGAGGCCTAAACTAGCAGCATTCTTGGAGGAAATCAACAAGATTGTGGCATACAAGGTAACCAAAGCTGATCCAAAGGAGATTGCTGCAGCGTACAAGAAGAAATTCCTGGAGCAACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0010731 GO:0016740 GO:0016765 GO:0019538 GO:0034641 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051186 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C_2 GST_N_3

Protein Analysis

226

Amino Acids

25.71

Weight (kDa)

5.83

Isoelectric Point (pI)

32.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_3	PF13417	28 - 93	5.5e-14	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	33 - 88	1.7e-08	Glutathione S-transferase, N-terminal domain
GST_C_5	PF16865	109 - 195	5.4e-08	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	131 - 195	1.5e-09	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02780 AT5G02780 AT5G02780 AT5G02790
fragaria_vesca	FvH4_6g14970 FvH4_6g14970
malus_domestica	MD04G1111400.v1.1 MD04G1111600.v1.1 MD04G1111700.v1.1 MD04G1112000.v1.1 MD04G1112100.v1.1 MD12G1129400.v1.1
prunus_persica	Prupe.6G244700_v2.0.a1
pyrus_communis	pycom04g10330 pycom04g10340 pycom12g12570
rosa_chinensis	RchiOBHm_Chr3g0468151 RchiOBHm_Chr3g0468161
rosa_laevigata	RLG00000024444
rosa_multiflora	Rmu_sc0001810.1_g000001 Rmu_sc0003659.1_g000008 Rmu_sc0005590.1_g000002 Rmu_sc0021707.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412980
rosa_rugosa	Rorug03G0093700 Rorug03G0093700
rosa_samantha	Rh3AG141600 Rh3AG141700 Rh3BG163100 Rh3CG163000 Rh3CG163200 Rh3DG163600 Rh3DG163800
rosa_wichuraiana	Rw3G013380 Rw3G013390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	196
AccI	GTMKAC	1 cut(s)	180
AciI	CCGC	1 cut(s)	115
AclWI	GGATC	2 cut(s)	131, 620
AcoI	YGGCCR	1 cut(s)	319
AcsI	RAATTY	2 cut(s)	437, 659
AfaI	GTAC	4 cut(s)	8, 86, 167, 650
AfiI	CCNNNNNNNGG	1 cut(s)	354
AflII	CTTAAG	1 cut(s)	431
AflIII	ACRYGT	1 cut(s)	531
AgsI	TTSAA	3 cut(s)	148, 376, 538
AjiI	CACGTC	1 cut(s)	532
AjnI	CCWGG	1 cut(s)	663
AluBI	AGCT	3 cut(s)	398, 554, 623
AluI	AGCT	3 cut(s)	398, 554, 623
Alw21I	GWGCWC	1 cut(s)	213
Alw26I	GTCTC	1 cut(s)	375
AlwI	GGATC	2 cut(s)	131, 620
AlwNI	CAGNNNCTG	1 cut(s)	158
AoxI	GGCC	5 cut(s)	154, 275, 319, 449, 560
ApeKI	GCWGC	4 cut(s)	395, 572, 641, 644
ApoI	RAATTY	2 cut(s)	437, 659
Asp700I	GAANNNNTTC	1 cut(s)	659
AspS9I	GGNCC	4 cut(s)	155, 275, 357, 449
AsuC2I	CCSGG	1 cut(s)	186
AsuII	TTCGAA	1 cut(s)	270
AvaII	GGWCC	1 cut(s)	357
BaeI	ACNNNNGTAYC	2 cut(s)	336, 369
BalI	TGGCCA	1 cut(s)	321
BanI	GGYRCC	1 cut(s)	196
BbsI	GAAGAC	1 cut(s)	511
Bbv12I	GWGCWC	1 cut(s)	213
BbvI	GCAGC	4 cut(s)	407, 584, 628, 656
BccI	CCATC	2 cut(s)	208, 440
BceAI	ACGGC	1 cut(s)	85
BciT130I	CCWGG	1 cut(s)	665
BclI	TGATCA	1 cut(s)	246
BcnI	CCSGG	1 cut(s)	186
BcoDI	GTCTC	1 cut(s)	375
BfaI	CTAG	2 cut(s)	129, 569
BfmI	CTRYAG	1 cut(s)	642
BfrI	CTTAAG	1 cut(s)	431
BglII	AGATCT	1 cut(s)	141
BisI	GCNGC	4 cut(s)	396, 573, 642, 645
BlsI	GCNGC	4 cut(s)	397, 574, 643, 646
Bme1390I	CCNGG	2 cut(s)	186, 665
Bme18I	GGWCC	1 cut(s)	357
BmgBI	CACGTC	1 cut(s)	532
BmgT120I	GGNCC	4 cut(s)	155, 275, 357, 449
BmiI	GGNNCC	2 cut(s)	198, 358
BmrFI	CCNGG	2 cut(s)	186, 665
BpiI	GAAGAC	1 cut(s)	511
BpmI	CTGGAG	1 cut(s)	227
Bpu14I	TTCGAA	1 cut(s)	270
BpuMI	CCSGG	1 cut(s)	186
BsaAI	YACGTR	1 cut(s)	88
BsaJI	CCNNGG	1 cut(s)	184
BsaXI	ACNNNNNCTCC	3 cut(s)	372, 402, 659
Bsc4I	CCNNNNNNNGG	1 cut(s)	354
BseBI	CCWGG	1 cut(s)	665
BseDI	CCNNGG	1 cut(s)	184
BseLI	CCNNNNNNNGG	1 cut(s)	354
BseMII	CTCAG	1 cut(s)	294
BseRI	GAGGAG	2 cut(s)	45, 48
BseXI	GCAGC	4 cut(s)	407, 584, 628, 656
BseYI	CCCAGC	1 cut(s)	554
BshFI	GGCC	5 cut(s)	156, 277, 321, 451, 562
BshNI	GGYRCC	1 cut(s)	196
BsiHKAI	GWGCWC	1 cut(s)	213
BsiSI	CCGG	1 cut(s)	186
BslFI	GGGAC	1 cut(s)	272
BslI	CCNNNNNNNGG	1 cut(s)	354
BsmAI	GTCTC	1 cut(s)	375
BsmFI	GGGAC	1 cut(s)	272
BsmI	GAATGC	1 cut(s)	575
BsnI	GGCC	5 cut(s)	156, 277, 321, 451, 562
Bsp119I	TTCGAA	1 cut(s)	270
Bsp1286I	GDGCHC	1 cut(s)	213
Bsp143I	GATC	5 cut(s)	123, 141, 241, 246, 625
BspACI	CCGC	1 cut(s)	115
BspANI	GGCC	5 cut(s)	156, 277, 321, 451, 562
BspCNI	CTCAG	1 cut(s)	295
BspLI	GGNNCC	2 cut(s)	198, 358
BspMAI	CTGCAG	1 cut(s)	646
BspPI	GGATC	2 cut(s)	131, 620
BspT104I	TTCGAA	1 cut(s)	270
BspT107I	GGYRCC	1 cut(s)	196
BspTI	CTTAAG	1 cut(s)	431
BssECI	CCNNGG	1 cut(s)	184
BssMI	GATC	5 cut(s)	123, 141, 241, 246, 625
Bst2UI	CCWGG	1 cut(s)	665
Bst4CI	ACNGT	3 cut(s)	33, 388, 675
BstAFI	CTTAAG	1 cut(s)	431
BstBAI	YACGTR	1 cut(s)	88
BstBI	TTCGAA	1 cut(s)	270
BstDEI	CTNAG	1 cut(s)	303
BstEII	GGTNACC	1 cut(s)	613
BstKTI	GATC	5 cut(s)	126, 144, 244, 249, 628
BstMAI	GTCTC	1 cut(s)	375
BstMBI	GATC	5 cut(s)	123, 141, 241, 246, 625
BstNI	CCWGG	1 cut(s)	665
BstPI	GGTNACC	1 cut(s)	613
BstSCI	CCNGG	2 cut(s)	184, 663
BstSFI	CTRYAG	1 cut(s)	642
BstSNI	TACGTA	1 cut(s)	88
BstV1I	GCAGC	4 cut(s)	407, 584, 628, 656
BstV2I	GAAGAC	1 cut(s)	511
BstX2I	RGATCY	1 cut(s)	141
BstYI	RGATCY	1 cut(s)	141
BsuRI	GGCC	5 cut(s)	156, 277, 321, 451, 562
BtrI	CACGTC	1 cut(s)	532
CaiI	CAGNNNCTG	1 cut(s)	158
Cfr13I	GGNCC	4 cut(s)	155, 275, 357, 449
Csp6I	GTAC	4 cut(s)	7, 85, 166, 649
CviAII	CATG	1 cut(s)	14
CviQI	GTAC	4 cut(s)	7, 85, 166, 649
DdeI	CTNAG	1 cut(s)	303
DpnI	GATC	5 cut(s)	125, 143, 243, 248, 627
DpnII	GATC	5 cut(s)	123, 141, 241, 246, 625
EaeI	YGGCCR	1 cut(s)	319
Eco105I	TACGTA	1 cut(s)	88
Eco147I	AGGCCT	1 cut(s)	562
Eco47I	GGWCC	1 cut(s)	357
Eco91I	GGTNACC	1 cut(s)	613
EcoO109I	RGGNCCY	3 cut(s)	155, 275, 357
EcoO65I	GGTNACC	1 cut(s)	613
EcoRII	CCWGG	1 cut(s)	663
FaeI	CATG	1 cut(s)	17
FaiI	YATR	8 cut(s)	15, 138, 339, 454, 485, 492, 543, 607
FaqI	GGGAC	1 cut(s)	272
FatI	CATG	1 cut(s)	13
FauI	CCCGC	1 cut(s)	108
FbaI	TGATCA	1 cut(s)	246
FblI	GTMKAC	1 cut(s)	180
Fnu4HI	GCNGC	4 cut(s)	396, 573, 642, 645
Fsp4HI	GCNGC	4 cut(s)	396, 573, 642, 645
FspBI	CTAG	2 cut(s)	129, 569
GluI	GCNGC	4 cut(s)	396, 573, 642, 645
GsaI	CCCAGC	1 cut(s)	558
GsuI	CTGGAG	1 cut(s)	227
HaeIII	GGCC	5 cut(s)	156, 277, 321, 451, 562
HapII	CCGG	1 cut(s)	186
Hin1II	CATG	1 cut(s)	17
HinfI	GANTC	1 cut(s)	38
HpaII	CCGG	1 cut(s)	186
Hpy166II	GTNNAC	2 cut(s)	181, 481
Hpy188I	TCNGA	2 cut(s)	246, 529
Hpy188III	TCNNGA	3 cut(s)	145, 206, 239
Hpy8I	GTNNAC	2 cut(s)	181, 481
HpyAV	CCTTC	2 cut(s)	266, 288
HpyCH4III	ACNGT	3 cut(s)	33, 388, 675
HpyCH4IV	ACGT	2 cut(s)	87, 531
HpyCH4V	TGCA	2 cut(s)	110, 644
HpyF3I	CTNAG	1 cut(s)	303
HpySE526I	ACGT	2 cut(s)	87, 531
Hsp92II	CATG	1 cut(s)	17
Ksp22I	TGATCA	1 cut(s)	246
Kzo9I	GATC	5 cut(s)	123, 141, 241, 246, 625
LmnI	GCTCC	2 cut(s)	208, 667
LpnPI	CCDG	8 cut(s)	138, 171, 191, 199, 315, 446, 540, 650
Lsp1109I	GCAGC	4 cut(s)	407, 584, 628, 656
MaeI	CTAG	2 cut(s)	129, 569
MaeII	ACGT	2 cut(s)	87, 531
MaeIII	GTNAC	1 cut(s)	613
MalI	GATC	5 cut(s)	125, 143, 243, 248, 627
MboI	GATC	5 cut(s)	123, 141, 241, 246, 625
MboII	GAAGA	3 cut(s)	449, 511, 667
MflI	RGATCY	1 cut(s)	141
MhlI	GDGCHC	1 cut(s)	213
MlsI	TGGCCA	1 cut(s)	321
MluCI	AATT	5 cut(s)	91, 326, 334, 437, 659
MluNI	TGGCCA	1 cut(s)	321
MmeI	TCCRAC	1 cut(s)	15
MnlI	CCTC	9 cut(s)	10, 36, 58, 63, 66, 69, 266, 552, 577
Mox20I	TGGCCA	1 cut(s)	321
MroXI	GAANNNNTTC	1 cut(s)	659
MscI	TGGCCA	1 cut(s)	321
MseI	TTAA	2 cut(s)	332, 432
Msp20I	TGGCCA	1 cut(s)	321
MspA1I	CMGCKG	3 cut(s)	115, 398, 554
MspCI	CTTAAG	1 cut(s)	431
MspI	CCGG	1 cut(s)	186
MspR9I	CCNGG	2 cut(s)	186, 665
Mva1269I	GAATGC	1 cut(s)	575
MvaI	CCWGG	1 cut(s)	665
NciI	CCSGG	1 cut(s)	186
NdeII	GATC	5 cut(s)	123, 141, 241, 246, 625
NlaIII	CATG	1 cut(s)	17
NlaIV	GGNNCC	2 cut(s)	198, 358
NspV	TTCGAA	1 cut(s)	270
PceI	AGGCCT	1 cut(s)	562
PctI	GAATGC	1 cut(s)	575
PdmI	GAANNNNTTC	1 cut(s)	659
PfeI	GAWTC	1 cut(s)	38
PfoI	TCCNGGA	1 cut(s)	663
PkrI	GCNGC	4 cut(s)	397, 574, 643, 646
Ppu21I	YACGTR	1 cut(s)	88
PpuMI	RGGWCCY	1 cut(s)	357
Psp5II	RGGWCCY	1 cut(s)	357
Psp6I	CCWGG	1 cut(s)	663
PspEI	GGTNACC	1 cut(s)	613
PspFI	CCCAGC	1 cut(s)	554
PspGI	CCWGG	1 cut(s)	663
PspN4I	GGNNCC	2 cut(s)	198, 358
PspPI	GGNCC	4 cut(s)	155, 275, 357, 449
PspPPI	RGGWCCY	1 cut(s)	357
PstI	CTGCAG	1 cut(s)	646
PstNI	CAGNNNCTG	1 cut(s)	158
PsuI	RGATCY	1 cut(s)	141
PvuII	CAGCTG	2 cut(s)	398, 554
RsaI	GTAC	4 cut(s)	8, 86, 167, 650
RsaNI	GTAC	4 cut(s)	7, 85, 166, 649
SaqAI	TTAA	2 cut(s)	332, 432
SatI	GCNGC	4 cut(s)	396, 573, 642, 645
Sau3AI	GATC	5 cut(s)	123, 141, 241, 246, 625
Sau96I	GGNCC	4 cut(s)	155, 275, 357, 449
ScrFI	CCNGG	2 cut(s)	186, 665
SduI	GDGCHC	1 cut(s)	213
SetI	ASST	9 cut(s)	90, 198, 258, 350, 400, 534, 556, 615, 625
SfcI	CTRYAG	1 cut(s)	642
SfuI	TTCGAA	1 cut(s)	270
SinI	GGWCC	1 cut(s)	357
SmlI	CTYRAG	1 cut(s)	431
SmoI	CTYRAG	1 cut(s)	431
SnaBI	TACGTA	1 cut(s)	88
Sse9I	AATT	5 cut(s)	91, 326, 334, 437, 659
SseBI	AGGCCT	1 cut(s)	562
SsiI	CCGC	1 cut(s)	115
SspMI	CTAG	2 cut(s)	129, 569
StuI	AGGCCT	1 cut(s)	562
StyD4I	CCNGG	2 cut(s)	184, 663
TaaI	ACNGT	3 cut(s)	33, 388, 675
TaiI	ACGT	2 cut(s)	90, 534
TaqI	TCGA	2 cut(s)	258, 270
TasI	AATT	5 cut(s)	91, 326, 334, 437, 659
TfiI	GAWTC	1 cut(s)	38
Tru1I	TTAA	2 cut(s)	332, 432
Tru9I	TTAA	2 cut(s)	332, 432
TseI	GCWGC	4 cut(s)	395, 572, 641, 644
Vha464I	CTTAAG	1 cut(s)	431
VpaK11BI	GGWCC	1 cut(s)	357
XapI	RAATTY	2 cut(s)	437, 659
XmiI	GTMKAC	1 cut(s)	180
XmnI	GAANNNNTTC	1 cut(s)	659
XspI	CTAG	2 cut(s)	129, 569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.