Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001353

Physical Location & Seq

Genomic Coordinates Forward (+)

26529867 .. 26530208

Gene Structure 342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1173800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 342 bp

ATGGCTAGCTCTGCAGTGATCAAGCTTGCTTTGGTGGTGGCCTTGTGCATGGCGGTGAGCGTTGCGCATGCCATAACATGTGGCCAGGTGAGCTCCAACCTTGCACCATGCATTGGCTACCTGAGGGGTGGCGGACCTGTCCCTCCAGCTTGCTGCAATGGAATCAGAACCATTAACAGCTTGGCCGAGACCACCCCTGACCGCCAGGCTGTTTGCAACTGCTTGAAGAGTCTTGCCAGCAGCGTCAGTGGTGTTAACCCTGGCAATGCTGAATCGCTTCCTGGAAAGTGTGGAGTCAACGTCCCCTACAAGATCAGCACCTCCACCAACTGCACCACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005319 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009505 GO:0009506 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009628 GO:0009889 GO:0009987 GO:0010035 GO:0010556 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0019222 GO:0030054 GO:0030154 GO:0030312 GO:0031410 GO:0031976 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0042221 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0060255 GO:0061024 GO:0065007 GO:0071702 GO:0071840 GO:0071944 GO:0090627 GO:0097708 GO:0098791 GO:1901700 GO:1901957

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

114

Amino Acids

11.3

Weight (kDa)

8.65

Isoelectric Point (pI)

34.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	11 - 104	7.5e-07	Probable lipid transfer
Tryp_alpha_amyl	PF00234	27 - 111	4.5e-17	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33355 AT4G33355
fragaria_vesca	FvH4_6g09970 FvH4_6g09980
malus_domestica	MD04G1173600.v1.1 MD04G1173800.v1.1 MD12G1187100.v1.1 MD12G1187200.v1.1 MD12G1187500.v1.1
prunus_persica	Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292800_v2.0.a1
pyrus_communis	pycom04g15340 pycom12g17540 pycom12g17550
rosa_chinensis	RchiOBHm_Chr3g0460591 RchiOBHm_Chr3g0460641 RchiOBHm_Chr3g0460651 RchiOBHm_Chr7g0211001
rosa_laevigata	RLG00000003030 RLG00000024990 RLG00000024991
rosa_multiflora	Rmu_co8436807.1_g000001 Rmu_sc0001256.1_g000005 Rmu_sc0001256.1_g000008 Rmu_sc0001256.1_g000009 Rmu_sc0003701.1_g000008 Rmu_sc0011069.1_g000012
rosa_roxburghii	Rroxscaffold_3G00248180 Rroxscaffold_6G00419330 Rroxscaffold_6G00419340
rosa_rugosa	Rorug03G0044200 Rorug03G0044300 Rorug03G0044400 Rorug03G0044500 Rorug07G0123800
rosa_samantha	Rh3AG102300 Rh3AG102400 Rh3BG105900 Rh3BG106000 Rh3BG106100 Rh3CG107900 Rh3CG108000 Rh3DG107400 Rh3DG107600 Rh7AG255400 Rh7BG249900 Rh7CG272000
rosa_wichuraiana	Rw0G010020 Rw3G008690 Rw3G008710 Rw7G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	66
AccB7I	CCANNNNNTGG	1 cut(s)	113
AciI	CCGC	3 cut(s)	53, 132, 202
AcoI	YGGCCR	2 cut(s)	82, 183
AfiI	CCNNNNNNNGG	1 cut(s)	113
AflIII	ACRYGT	1 cut(s)	77
AgsI	TTSAA	1 cut(s)	226
AjnI	CCWGG	4 cut(s)	84, 204, 259, 280
AluBI	AGCT	5 cut(s)	9, 25, 93, 149, 180
AluI	AGCT	5 cut(s)	9, 25, 93, 149, 180
Alw21I	GWGCWC	1 cut(s)	95
Alw26I	GTCTC	1 cut(s)	182
AoxI	GGCC	3 cut(s)	39, 82, 183
ApeKI	GCWGC	2 cut(s)	153, 240
Asp700I	GAANNNNTTC	1 cut(s)	276
AspLEI	GCGC	1 cut(s)	67
AspS9I	GGNCC	1 cut(s)	134
AsuHPI	GGTGA	2 cut(s)	67, 100
AsuNHI	GCTAGC	1 cut(s)	5
AvaII	GGWCC	1 cut(s)	134
AxyI	CCTNAGG	1 cut(s)	122
BalI	TGGCCA	1 cut(s)	84
BanII	GRGCYC	1 cut(s)	95
Bbv12I	GWGCWC	1 cut(s)	95
BbvI	GCAGC	2 cut(s)	140, 252
BciT130I	CCWGG	4 cut(s)	86, 206, 261, 282
BclI	TGATCA	1 cut(s)	18
BcoDI	GTCTC	1 cut(s)	182
BfaI	CTAG	1 cut(s)	6
BfmI	CTRYAG	1 cut(s)	12
BisI	GCNGC	2 cut(s)	154, 241
BlsI	GCNGC	2 cut(s)	155, 242
Bme1390I	CCNGG	4 cut(s)	86, 206, 261, 282
Bme18I	GGWCC	1 cut(s)	134
BmgT120I	GGNCC	1 cut(s)	134
BmrFI	CCNGG	4 cut(s)	86, 206, 261, 282
BmtI	GCTAGC	1 cut(s)	9
BpmI	CTGGAG	1 cut(s)	129
BsaAI	YACGTR	1 cut(s)	339
BsaI	GGTCTC	1 cut(s)	182
BsaJI	CCNNGG	1 cut(s)	259
BsaXI	ACNNNNNCTCC	2 cut(s)	285, 315
Bsc4I	CCNNNNNNNGG	1 cut(s)	113
Bse21I	CCTNAGG	1 cut(s)	122
Bse3DI	GCAATG	2 cut(s)	163, 271
BseBI	CCWGG	4 cut(s)	86, 206, 261, 282
BseDI	CCNNGG	1 cut(s)	259
BseLI	CCNNNNNNNGG	1 cut(s)	113
BseMI	GCAATG	2 cut(s)	163, 271
BseMII	CTCAG	1 cut(s)	113
BseXI	GCAGC	2 cut(s)	140, 252
BsgI	GTGCAG	1 cut(s)	316
BshFI	GGCC	3 cut(s)	41, 84, 185
BsiHKAI	GWGCWC	1 cut(s)	95
BslFI	GGGAC	2 cut(s)	125, 287
BslI	CCNNNNNNNGG	1 cut(s)	113
BsmAI	GTCTC	1 cut(s)	182
BsmFI	GGGAC	2 cut(s)	125, 287
BsnI	GGCC	3 cut(s)	41, 84, 185
Bso31I	GGTCTC	1 cut(s)	182
Bsp1286I	GDGCHC	1 cut(s)	95
Bsp143I	GATC	2 cut(s)	18, 312
BspACI	CCGC	3 cut(s)	53, 132, 202
BspANI	GGCC	3 cut(s)	41, 84, 185
BspCNI	CTCAG	1 cut(s)	114
BspMAI	CTGCAG	1 cut(s)	16
BspOI	GCTAGC	1 cut(s)	9
BspTNI	GGTCTC	1 cut(s)	182
BsrDI	GCAATG	2 cut(s)	163, 271
BssECI	CCNNGG	1 cut(s)	259
BssMI	GATC	2 cut(s)	18, 312
Bst2UI	CCWGG	4 cut(s)	86, 206, 261, 282
Bst6I	CTCTTC	1 cut(s)	221
BstBAI	YACGTR	1 cut(s)	339
BstC8I	GCNNGC	5 cut(s)	7, 27, 69, 151, 238
BstDEI	CTNAG	1 cut(s)	122
BstHHI	GCGC	1 cut(s)	67
BstKTI	GATC	2 cut(s)	21, 315
BstMAI	GTCTC	1 cut(s)	182
BstMBI	GATC	2 cut(s)	18, 312
BstMWI	GCNNNNNNNGC	2 cut(s)	11, 90
BstNI	CCWGG	4 cut(s)	86, 206, 261, 282
BstNSI	RCATGY	2 cut(s)	71, 81
BstSCI	CCNGG	4 cut(s)	84, 204, 259, 280
BstSFI	CTRYAG	1 cut(s)	12
BstV1I	GCAGC	2 cut(s)	140, 252
Bsu36I	CCTNAGG	1 cut(s)	122
BsuRI	GGCC	3 cut(s)	41, 84, 185
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	2 cut(s)	21, 253
Cac8I	GCNNGC	5 cut(s)	7, 27, 69, 151, 238
CfoI	GCGC	1 cut(s)	67
Cfr13I	GGNCC	1 cut(s)	134
CseI	GACGC	1 cut(s)	232
CviAII	CATG	4 cut(s)	49, 68, 78, 108
DdeI	CTNAG	1 cut(s)	122
DpnI	GATC	2 cut(s)	20, 314
DpnII	GATC	2 cut(s)	18, 312
EaeI	YGGCCR	2 cut(s)	82, 183
Eam1104I	CTCTTC	1 cut(s)	221
EarI	CTCTTC	1 cut(s)	221
EciI	GGCGGA	1 cut(s)	147
Ecl136II	GAGCTC	1 cut(s)	93
Eco24I	GRGCYC	1 cut(s)	95
Eco31I	GGTCTC	1 cut(s)	182
Eco47I	GGWCC	1 cut(s)	134
Eco53kI	GAGCTC	1 cut(s)	93
Eco81I	CCTNAGG	1 cut(s)	122
EcoICRI	GAGCTC	1 cut(s)	93
EcoRII	CCWGG	4 cut(s)	84, 204, 259, 280
EcoT22I	ATGCAT	1 cut(s)	113
EcoT38I	GRGCYC	1 cut(s)	95
FaeI	CATG	4 cut(s)	52, 71, 81, 111
FaiI	YATR	5 cut(s)	50, 69, 74, 79, 109
FaqI	GGGAC	2 cut(s)	125, 287
FatI	CATG	4 cut(s)	48, 67, 77, 107
FbaI	TGATCA	1 cut(s)	18
Fnu4HI	GCNGC	2 cut(s)	154, 241
FriOI	GRGCYC	1 cut(s)	95
Fsp4HI	GCNGC	2 cut(s)	154, 241
FspBI	CTAG	1 cut(s)	6
FspI	TGCGCA	1 cut(s)	66
GlaI	GCGC	1 cut(s)	66
GluI	GCNGC	2 cut(s)	154, 241
GsuI	CTGGAG	1 cut(s)	129
HaeIII	GGCC	3 cut(s)	41, 84, 185
HgaI	GACGC	1 cut(s)	232
HhaI	GCGC	1 cut(s)	67
Hin1II	CATG	4 cut(s)	52, 71, 81, 111
Hin6I	GCGC	1 cut(s)	65
HinP1I	GCGC	1 cut(s)	65
HincII	GTYRAC	2 cut(s)	256, 298
HindII	GTYRAC	2 cut(s)	256, 298
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	4 cut(s)	162, 229, 272, 294
HpaI	GTTAAC	1 cut(s)	256
HphI	GGTGA	2 cut(s)	67, 100
Hpy166II	GTNNAC	2 cut(s)	256, 298
Hpy188I	TCNGA	1 cut(s)	167
Hpy8I	GTNNAC	2 cut(s)	256, 298
HpyCH4IV	ACGT	2 cut(s)	300, 338
HpyCH4V	TGCA	7 cut(s)	14, 48, 104, 111, 156, 216, 333
HpyF10VI	GCNNNNNNNGC	2 cut(s)	11, 90
HpyF3I	CTNAG	1 cut(s)	122
HpySE526I	ACGT	2 cut(s)	300, 338
Hsp92II	CATG	4 cut(s)	52, 71, 81, 111
HspAI	GCGC	1 cut(s)	65
Ksp22I	TGATCA	1 cut(s)	18
KspAI	GTTAAC	1 cut(s)	256
Kzo9I	GATC	2 cut(s)	18, 312
LmnI	GCTCC	1 cut(s)	98
Lsp1109I	GCAGC	2 cut(s)	140, 252
MaeI	CTAG	1 cut(s)	6
MaeII	ACGT	2 cut(s)	300, 338
MalI	GATC	2 cut(s)	20, 314
MboI	GATC	2 cut(s)	18, 312
MboII	GAAGA	1 cut(s)	238
MhlI	GDGCHC	1 cut(s)	95
MlsI	TGGCCA	1 cut(s)	84
MluNI	TGGCCA	1 cut(s)	84
MlyI	GAGTC	2 cut(s)	238, 303
MmeI	TCCRAC	1 cut(s)	120
MnlI	CCTC	3 cut(s)	117, 153, 331
Mox20I	TGGCCA	1 cut(s)	84
Mph1103I	ATGCAT	1 cut(s)	113
MroXI	GAANNNNTTC	1 cut(s)	276
MscI	TGGCCA	1 cut(s)	84
MseI	TTAA	2 cut(s)	174, 255
MslI	CAYNNNNRTG	1 cut(s)	53
Msp20I	TGGCCA	1 cut(s)	84
MspR9I	CCNGG	4 cut(s)	86, 206, 261, 282
MvaI	CCWGG	4 cut(s)	86, 206, 261, 282
MwoI	GCNNNNNNNGC	2 cut(s)	11, 90
NdeII	GATC	2 cut(s)	18, 312
NheI	GCTAGC	1 cut(s)	5
NlaIII	CATG	4 cut(s)	52, 71, 81, 111
NmeAIII	GCCGAG	1 cut(s)	211
NsbI	TGCGCA	1 cut(s)	66
NsiI	ATGCAT	1 cut(s)	113
NspI	RCATGY	2 cut(s)	71, 81
PaeI	GCATGC	1 cut(s)	71
PciI	ACATGT	1 cut(s)	77
PdmI	GAANNNNTTC	1 cut(s)	276
PfeI	GAWTC	2 cut(s)	162, 272
PflMI	CCANNNNNTGG	1 cut(s)	113
PfoI	TCCNGGA	1 cut(s)	280
PkrI	GCNGC	2 cut(s)	155, 242
PleI	GAGTC	2 cut(s)	237, 302
PpsI	GAGTC	2 cut(s)	237, 302
Ppu21I	YACGTR	1 cut(s)	339
PscI	ACATGT	1 cut(s)	77
Psp124BI	GAGCTC	1 cut(s)	95
Psp6I	CCWGG	4 cut(s)	84, 204, 259, 280
PspGI	CCWGG	4 cut(s)	84, 204, 259, 280
PspPI	GGNCC	1 cut(s)	134
PstI	CTGCAG	1 cut(s)	16
RseI	CAYNNNNRTG	1 cut(s)	53
SacI	GAGCTC	1 cut(s)	95
SaqAI	TTAA	2 cut(s)	174, 255
SatI	GCNGC	2 cut(s)	154, 241
Sau3AI	GATC	2 cut(s)	18, 312
Sau96I	GGNCC	1 cut(s)	134
SchI	GAGTC	2 cut(s)	238, 303
ScrFI	CCNGG	4 cut(s)	86, 206, 261, 282
SduI	GDGCHC	1 cut(s)	95
SfcI	CTRYAG	1 cut(s)	12
SinI	GGWCC	1 cut(s)	134
SmiMI	CAYNNNNRTG	1 cut(s)	53
SphI	GCATGC	1 cut(s)	71
SsiI	CCGC	3 cut(s)	53, 132, 202
SspMI	CTAG	1 cut(s)	6
SstI	GAGCTC	1 cut(s)	95
StyD4I	CCNGG	4 cut(s)	84, 204, 259, 280
TaiI	ACGT	2 cut(s)	303, 341
TfiI	GAWTC	2 cut(s)	162, 272
Tru1I	TTAA	2 cut(s)	174, 255
Tru9I	TTAA	2 cut(s)	174, 255
TscAI	CASTG	2 cut(s)	21, 253
TseI	GCWGC	2 cut(s)	153, 240
TspRI	CASTG	2 cut(s)	21, 253
Van91I	CCANNNNNTGG	1 cut(s)	113
VpaK11BI	GGWCC	1 cut(s)	134
XceI	RCATGY	2 cut(s)	71, 81
XcmI	CCANNNNNNNNNTGG	1 cut(s)	178
XmnI	GAANNNNTTC	1 cut(s)	276
XspI	CTAG	1 cut(s)	6
Zsp2I	ATGCAT	1 cut(s)	113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.