Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001353

Physical Location & Seq

Genomic Coordinates Forward (+)

26930672 .. 26931629

Gene Structure 958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1187100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 348 bp

ATGGCTAGCTCTGCAGTGACCAAGCTTGCTTTGGTGGTGGCCTTGTGCATGGCGGTGAGCGTTGCTCATGCCATAACATGTGGCCAAGTGACCAGCAGCCTTGCGCCATGCATTGGCTACGTGAGGAGTGGCGGAGCTGTCCCTCCAGCTTGCTGCAATGGAATCAGAACCATTAACGGCTTGGCCAGGACCACCGCTGACCGCCAGACTGCTTGCAACTGCCTGAAGAATCTTGCCGGCAGCATCAGTGGTGTTAACCCTAACAATGCAGCAGGGCTTCCTGGAAAGTGTGGAGTCAACGTCCCCTACAAGATCAGCACCTCCACCAACTGCGCCACCGTGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005319 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009505 GO:0009506 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009628 GO:0009889 GO:0009987 GO:0010035 GO:0010556 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0019222 GO:0030054 GO:0030154 GO:0030312 GO:0031410 GO:0031976 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0042221 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0060255 GO:0061024 GO:0065007 GO:0071702 GO:0071840 GO:0071944 GO:0090627 GO:0097708 GO:0098791 GO:1901700 GO:1901957

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

116

Amino Acids

11.41

Weight (kDa)

9.28

Isoelectric Point (pI)

29.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	11 - 104	4.5e-07	Probable lipid transfer
Tryp_alpha_amyl	PF00234	27 - 111	7.4e-17	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33355 AT4G33355
fragaria_vesca	FvH4_6g09970 FvH4_6g09980
malus_domestica	MD04G1173600.v1.1 MD04G1173800.v1.1 MD12G1187100.v1.1 MD12G1187200.v1.1 MD12G1187500.v1.1
prunus_persica	Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292800_v2.0.a1
pyrus_communis	pycom04g15340 pycom12g17540 pycom12g17550
rosa_chinensis	RchiOBHm_Chr3g0460591 RchiOBHm_Chr3g0460641 RchiOBHm_Chr3g0460651 RchiOBHm_Chr7g0211001
rosa_laevigata	RLG00000003030 RLG00000024990 RLG00000024991
rosa_multiflora	Rmu_co8436807.1_g000001 Rmu_sc0001256.1_g000005 Rmu_sc0001256.1_g000008 Rmu_sc0001256.1_g000009 Rmu_sc0003701.1_g000008 Rmu_sc0011069.1_g000012
rosa_roxburghii	Rroxscaffold_3G00248180 Rroxscaffold_6G00419330 Rroxscaffold_6G00419340
rosa_rugosa	Rorug03G0044200 Rorug03G0044300 Rorug03G0044400 Rorug03G0044500 Rorug07G0123800
rosa_samantha	Rh3AG102300 Rh3AG102400 Rh3BG105900 Rh3BG106000 Rh3BG106100 Rh3CG107900 Rh3CG108000 Rh3DG107400 Rh3DG107600 Rh7AG255400 Rh7BG249900 Rh7CG272000
rosa_wichuraiana	Rw0G010020 Rw3G008690 Rw3G008710 Rw7G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	113
AciI	CCGC	4 cut(s)	53, 132, 195, 202
AcoI	YGGCCR	2 cut(s)	82, 183
AcuI	CTGAAG	1 cut(s)	245
AfiI	CCNNNNNNNGG	1 cut(s)	113
AflIII	ACRYGT	1 cut(s)	77
AjnI	CCWGG	2 cut(s)	185, 280
AluBI	AGCT	4 cut(s)	9, 25, 137, 149
AluI	AGCT	4 cut(s)	9, 25, 137, 149
AoxI	GGCC	3 cut(s)	39, 82, 183
ApeKI	GCWGC	4 cut(s)	96, 153, 240, 269
AspLEI	GCGC	2 cut(s)	106, 335
AspS9I	GGNCC	1 cut(s)	189
AsuHPI	GGTGA	1 cut(s)	67
AsuNHI	GCTAGC	1 cut(s)	5
AvaII	GGWCC	1 cut(s)	189
BalI	TGGCCA	2 cut(s)	84, 185
BbvI	GCAGC	4 cut(s)	108, 140, 252, 281
BceAI	ACGGC	1 cut(s)	193
BciT130I	CCWGG	2 cut(s)	187, 282
BfaI	CTAG	1 cut(s)	6
BfmI	CTRYAG	1 cut(s)	12
BisI	GCNGC	4 cut(s)	97, 154, 241, 270
BlsI	GCNGC	4 cut(s)	98, 155, 242, 271
Bme1390I	CCNGG	2 cut(s)	187, 282
Bme18I	GGWCC	1 cut(s)	189
BmgT120I	GGNCC	1 cut(s)	189
BmrFI	CCNGG	2 cut(s)	187, 282
BmsI	GCATC	1 cut(s)	252
BmtI	GCTAGC	1 cut(s)	9
BplI	GAGNNNNNCTC	2 cut(s)	49, 81
BpmI	CTGGAG	1 cut(s)	129
BsaAI	YACGTR	1 cut(s)	121
BsaXI	ACNNNNNCTCC	2 cut(s)	285, 315
Bsc4I	CCNNNNNNNGG	1 cut(s)	113
Bse118I	RCCGGY	1 cut(s)	236
Bse3DI	GCAATG	1 cut(s)	163
BseBI	CCWGG	2 cut(s)	187, 282
BseLI	CCNNNNNNNGG	1 cut(s)	113
BseMI	GCAATG	1 cut(s)	163
BseRI	GAGGAG	1 cut(s)	139
BseXI	GCAGC	4 cut(s)	108, 140, 252, 281
BshFI	GGCC	3 cut(s)	41, 84, 185
BsiSI	CCGG	1 cut(s)	237
BslFI	GGGAC	2 cut(s)	125, 287
BslI	CCNNNNNNNGG	1 cut(s)	113
BsmFI	GGGAC	2 cut(s)	125, 287
BsnI	GGCC	3 cut(s)	41, 84, 185
Bsp143I	GATC	1 cut(s)	312
BspACI	CCGC	4 cut(s)	53, 132, 195, 202
BspANI	GGCC	3 cut(s)	41, 84, 185
BspMAI	CTGCAG	1 cut(s)	16
BspOI	GCTAGC	1 cut(s)	9
BsrDI	GCAATG	1 cut(s)	163
BsrFI	RCCGGY	1 cut(s)	236
BssAI	RCCGGY	1 cut(s)	236
BssMI	GATC	1 cut(s)	312
Bst2UI	CCWGG	2 cut(s)	187, 282
Bst4CI	ACNGT	1 cut(s)	340
BstBAI	YACGTR	1 cut(s)	121
BstC8I	GCNNGC	5 cut(s)	7, 27, 151, 214, 238
BstHHI	GCGC	2 cut(s)	106, 335
BstKTI	GATC	1 cut(s)	315
BstMBI	GATC	1 cut(s)	312
BstMWI	GCNNNNNNNGC	1 cut(s)	11
BstNI	CCWGG	2 cut(s)	187, 282
BstNSI	RCATGY	1 cut(s)	81
BstSCI	CCNGG	2 cut(s)	185, 280
BstSFI	CTRYAG	1 cut(s)	12
BstV1I	GCAGC	4 cut(s)	108, 140, 252, 281
BsuRI	GGCC	3 cut(s)	41, 84, 185
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	2 cut(s)	21, 253
Cac8I	GCNNGC	5 cut(s)	7, 27, 151, 214, 238
CfoI	GCGC	2 cut(s)	106, 335
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	1 cut(s)	189
CviAII	CATG	4 cut(s)	49, 68, 78, 108
DpnI	GATC	1 cut(s)	314
DpnII	GATC	1 cut(s)	312
EaeI	YGGCCR	2 cut(s)	82, 183
EciI	GGCGGA	1 cut(s)	147
Eco47I	GGWCC	1 cut(s)	189
Eco57I	CTGAAG	1 cut(s)	245
EcoRII	CCWGG	2 cut(s)	185, 280
EcoT22I	ATGCAT	1 cut(s)	113
FaeI	CATG	4 cut(s)	52, 71, 81, 111
FaiI	YATR	5 cut(s)	50, 69, 74, 79, 109
FaqI	GGGAC	2 cut(s)	125, 287
FatI	CATG	4 cut(s)	48, 67, 77, 107
Fnu4HI	GCNGC	4 cut(s)	97, 154, 241, 270
Fsp4HI	GCNGC	4 cut(s)	97, 154, 241, 270
FspBI	CTAG	1 cut(s)	6
GlaI	GCGC	2 cut(s)	105, 334
GluI	GCNGC	4 cut(s)	97, 154, 241, 270
GsuI	CTGGAG	1 cut(s)	129
HaeIII	GGCC	3 cut(s)	41, 84, 185
HapII	CCGG	1 cut(s)	237
HhaI	GCGC	2 cut(s)	106, 335
Hin1II	CATG	4 cut(s)	52, 71, 81, 111
Hin6I	GCGC	2 cut(s)	104, 333
HinP1I	GCGC	2 cut(s)	104, 333
HincII	GTYRAC	2 cut(s)	256, 298
HindII	GTYRAC	2 cut(s)	256, 298
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	3 cut(s)	162, 229, 294
HpaI	GTTAAC	1 cut(s)	256
HpaII	CCGG	1 cut(s)	237
HphI	GGTGA	1 cut(s)	67
Hpy166II	GTNNAC	2 cut(s)	256, 298
Hpy188I	TCNGA	1 cut(s)	167
Hpy8I	GTNNAC	2 cut(s)	256, 298
HpyCH4III	ACNGT	1 cut(s)	340
HpyCH4IV	ACGT	2 cut(s)	120, 300
HpyCH4V	TGCA	6 cut(s)	14, 48, 111, 156, 216, 269
HpyF10VI	GCNNNNNNNGC	1 cut(s)	11
HpySE526I	ACGT	2 cut(s)	120, 300
Hsp92II	CATG	4 cut(s)	52, 71, 81, 111
HspAI	GCGC	2 cut(s)	104, 333
KroI	GCCGGC	1 cut(s)	236
KroNI	GCCGGC	1 cut(s)	238
KspAI	GTTAAC	1 cut(s)	256
Kzo9I	GATC	1 cut(s)	312
LmnI	GCTCC	1 cut(s)	134
Lsp1109I	GCAGC	4 cut(s)	108, 140, 252, 281
LweI	GCATC	1 cut(s)	252
MaeI	CTAG	1 cut(s)	6
MaeII	ACGT	2 cut(s)	120, 300
MaeIII	GTNAC	2 cut(s)	16, 88
MalI	GATC	1 cut(s)	314
MboI	GATC	1 cut(s)	312
MboII	GAAGA	1 cut(s)	238
MlsI	TGGCCA	2 cut(s)	84, 185
MluNI	TGGCCA	2 cut(s)	84, 185
MlyI	GAGTC	1 cut(s)	303
MnlI	CCTC	3 cut(s)	117, 153, 331
Mox20I	TGGCCA	2 cut(s)	84, 185
Mph1103I	ATGCAT	1 cut(s)	113
MroNI	GCCGGC	1 cut(s)	236
MscI	TGGCCA	2 cut(s)	84, 185
MseI	TTAA	2 cut(s)	174, 255
MslI	CAYNNNNRTG	1 cut(s)	53
Msp20I	TGGCCA	2 cut(s)	84, 185
MspA1I	CMGCKG	1 cut(s)	197
MspI	CCGG	1 cut(s)	237
MspR9I	CCNGG	2 cut(s)	187, 282
MvaI	CCWGG	2 cut(s)	187, 282
MwoI	GCNNNNNNNGC	1 cut(s)	11
NaeI	GCCGGC	1 cut(s)	238
NdeII	GATC	1 cut(s)	312
NgoMIV	GCCGGC	1 cut(s)	236
NheI	GCTAGC	1 cut(s)	5
NlaIII	CATG	4 cut(s)	52, 71, 81, 111
NmuCI	GTSAC	2 cut(s)	16, 88
NsiI	ATGCAT	1 cut(s)	113
NspI	RCATGY	1 cut(s)	81
PciI	ACATGT	1 cut(s)	77
PdiI	GCCGGC	1 cut(s)	238
PfeI	GAWTC	2 cut(s)	162, 229
PflMI	CCANNNNNTGG	1 cut(s)	113
PfoI	TCCNGGA	1 cut(s)	280
PkrI	GCNGC	4 cut(s)	98, 155, 242, 271
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Ppu21I	YACGTR	1 cut(s)	121
PscI	ACATGT	1 cut(s)	77
Psp6I	CCWGG	2 cut(s)	185, 280
PspGI	CCWGG	2 cut(s)	185, 280
PspPI	GGNCC	1 cut(s)	189
PstI	CTGCAG	1 cut(s)	16
RseI	CAYNNNNRTG	1 cut(s)	53
SaqAI	TTAA	2 cut(s)	174, 255
SatI	GCNGC	4 cut(s)	97, 154, 241, 270
Sau3AI	GATC	1 cut(s)	312
Sau96I	GGNCC	1 cut(s)	189
SchI	GAGTC	1 cut(s)	303
ScrFI	CCNGG	2 cut(s)	187, 282
SetI	ASST	7 cut(s)	11, 27, 123, 139, 151, 303, 323
SfaNI	GCATC	1 cut(s)	252
SfcI	CTRYAG	1 cut(s)	12
SinI	GGWCC	1 cut(s)	189
SmiMI	CAYNNNNRTG	1 cut(s)	53
SsiI	CCGC	4 cut(s)	53, 132, 195, 202
SspMI	CTAG	1 cut(s)	6
StyD4I	CCNGG	2 cut(s)	185, 280
TaaI	ACNGT	1 cut(s)	340
TaiI	ACGT	2 cut(s)	123, 303
TfiI	GAWTC	2 cut(s)	162, 229
Tru1I	TTAA	2 cut(s)	174, 255
Tru9I	TTAA	2 cut(s)	174, 255
TscAI	CASTG	2 cut(s)	21, 253
TseFI	GTSAC	2 cut(s)	16, 88
TseI	GCWGC	4 cut(s)	96, 153, 240, 269
Tsp45I	GTSAC	2 cut(s)	16, 88
TspRI	CASTG	2 cut(s)	21, 253
Van91I	CCANNNNNTGG	1 cut(s)	113
VpaK11BI	GGWCC	1 cut(s)	189
XceI	RCATGY	1 cut(s)	81
XcmI	CCANNNNNNNNNTGG	2 cut(s)	28, 178
XspI	CTAG	1 cut(s)	6
Zsp2I	ATGCAT	1 cut(s)	113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.