Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001353

Physical Location & Seq

Genomic Coordinates Forward (+)

26942088 .. 26942429

Gene Structure 342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1187200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 342 bp

ATGGCTAGCTCTGCAGTGACCAAGCTTTCTTTGGTGGTGGCCTTGTGCATGTCGGTGAGCGTTGTTCATGCCATGACATGTGGACAAGTGACTAGCAACCTTGGGCCATGCATTAACTACGTGAGGCGTGGCGGAGCTGTCCCTCCAGCTTGCTGCAATGGAATCAGAACCATTAACGGCTTGGCCAGGACCACCGCTGATCGCCAGACTACCTGCAACTGCCTGAAGAATCTTGCCGGTAGCATCAGTGGTGTTAACCCTAACAATGCAGCAGCACTTCCTGTAAAGTGTGGAGTCAACGTCCCCTTCAAGATCAGCACCTCCACCAACTGCGCCACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005319 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009505 GO:0009506 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009628 GO:0009889 GO:0009987 GO:0010035 GO:0010556 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0019222 GO:0030054 GO:0030154 GO:0030312 GO:0031410 GO:0031976 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0042221 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0060255 GO:0061024 GO:0065007 GO:0071702 GO:0071840 GO:0071944 GO:0090627 GO:0097708 GO:0098791 GO:1901700 GO:1901957

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

114

Amino Acids

11.47

Weight (kDa)

9.33

Isoelectric Point (pI)

29.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	12 - 103	7.3e-07	Probable lipid transfer
Tryp_alpha_amyl	PF00234	27 - 111	1.5e-15	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33355 AT4G33355
fragaria_vesca	FvH4_6g09970 FvH4_6g09980
malus_domestica	MD04G1173600.v1.1 MD04G1173800.v1.1 MD12G1187100.v1.1 MD12G1187200.v1.1 MD12G1187500.v1.1
prunus_persica	Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292800_v2.0.a1
pyrus_communis	pycom04g15340 pycom12g17540 pycom12g17550
rosa_chinensis	RchiOBHm_Chr3g0460591 RchiOBHm_Chr3g0460641 RchiOBHm_Chr3g0460651 RchiOBHm_Chr7g0211001
rosa_laevigata	RLG00000003030 RLG00000024990 RLG00000024991
rosa_multiflora	Rmu_co8436807.1_g000001 Rmu_sc0001256.1_g000005 Rmu_sc0001256.1_g000008 Rmu_sc0001256.1_g000009 Rmu_sc0003701.1_g000008 Rmu_sc0011069.1_g000012
rosa_roxburghii	Rroxscaffold_3G00248180 Rroxscaffold_6G00419330 Rroxscaffold_6G00419340
rosa_rugosa	Rorug03G0044200 Rorug03G0044300 Rorug03G0044400 Rorug03G0044500 Rorug07G0123800
rosa_samantha	Rh3AG102300 Rh3AG102400 Rh3BG105900 Rh3BG106000 Rh3BG106100 Rh3CG107900 Rh3CG108000 Rh3DG107400 Rh3DG107600 Rh7AG255400 Rh7BG249900 Rh7CG272000
rosa_wichuraiana	Rw0G010020 Rw3G008690 Rw3G008710 Rw7G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	221
AciI	CCGC	2 cut(s)	132, 195
AcoI	YGGCCR	1 cut(s)	183
AcuI	CTGAAG	1 cut(s)	245
AflIII	ACRYGT	1 cut(s)	77
AgsI	TTSAA	1 cut(s)	310
AjnI	CCWGG	1 cut(s)	185
AluBI	AGCT	4 cut(s)	9, 25, 137, 149
AluI	AGCT	4 cut(s)	9, 25, 137, 149
AoxI	GGCC	3 cut(s)	39, 104, 183
ApeKI	GCWGC	3 cut(s)	153, 269, 272
AspLEI	GCGC	1 cut(s)	335
AspS9I	GGNCC	2 cut(s)	104, 189
AsuHPI	GGTGA	1 cut(s)	67
AsuNHI	GCTAGC	1 cut(s)	5
AvaII	GGWCC	1 cut(s)	189
BalI	TGGCCA	1 cut(s)	185
BbvI	GCAGC	3 cut(s)	140, 281, 284
BceAI	ACGGC	1 cut(s)	193
BciT130I	CCWGG	1 cut(s)	187
BfaI	CTAG	2 cut(s)	6, 93
BfmI	CTRYAG	1 cut(s)	12
BfuAI	ACCTGC	1 cut(s)	221
BisI	GCNGC	3 cut(s)	154, 270, 273
BlsI	GCNGC	3 cut(s)	155, 271, 274
Bme1390I	CCNGG	1 cut(s)	187
Bme18I	GGWCC	1 cut(s)	189
BmgT120I	GGNCC	2 cut(s)	104, 189
BmrFI	CCNGG	1 cut(s)	187
BmsI	GCATC	1 cut(s)	252
BmtI	GCTAGC	1 cut(s)	9
BpmI	CTGGAG	1 cut(s)	129
BsaAI	YACGTR	2 cut(s)	121, 339
BsaJI	CCNNGG	1 cut(s)	100
BsaXI	ACNNNNNCTCC	2 cut(s)	285, 315
Bse118I	RCCGGY	1 cut(s)	236
Bse3DI	GCAATG	1 cut(s)	163
BseBI	CCWGG	1 cut(s)	187
BseDI	CCNNGG	1 cut(s)	100
BseMI	GCAATG	1 cut(s)	163
BseXI	GCAGC	3 cut(s)	140, 281, 284
BshFI	GGCC	3 cut(s)	41, 106, 185
BsiSI	CCGG	1 cut(s)	237
BslFI	GGGAC	2 cut(s)	125, 287
BsmFI	GGGAC	2 cut(s)	125, 287
BsnI	GGCC	3 cut(s)	41, 106, 185
Bsp143I	GATC	2 cut(s)	199, 312
BspACI	CCGC	2 cut(s)	132, 195
BspANI	GGCC	3 cut(s)	41, 106, 185
BspMAI	CTGCAG	1 cut(s)	16
BspMI	ACCTGC	1 cut(s)	221
BspOI	GCTAGC	1 cut(s)	9
BsrDI	GCAATG	1 cut(s)	163
BsrFI	RCCGGY	1 cut(s)	236
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	1 cut(s)	100
BssMI	GATC	2 cut(s)	199, 312
BssT1I	CCWWGG	1 cut(s)	100
Bst2UI	CCWGG	1 cut(s)	187
BstBAI	YACGTR	2 cut(s)	121, 339
BstC8I	GCNNGC	2 cut(s)	7, 151
BstHHI	GCGC	1 cut(s)	335
BstKTI	GATC	2 cut(s)	202, 315
BstMBI	GATC	2 cut(s)	199, 312
BstMWI	GCNNNNNNNGC	1 cut(s)	11
BstNI	CCWGG	1 cut(s)	187
BstNSI	RCATGY	2 cut(s)	52, 81
BstSCI	CCNGG	1 cut(s)	185
BstSFI	CTRYAG	1 cut(s)	12
BstV1I	GCAGC	3 cut(s)	140, 281, 284
BsuRI	GGCC	3 cut(s)	41, 106, 185
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	2 cut(s)	21, 253
BveI	ACCTGC	1 cut(s)	221
Cac8I	GCNNGC	2 cut(s)	7, 151
CfoI	GCGC	1 cut(s)	335
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	2 cut(s)	104, 189
CviAII	CATG	5 cut(s)	49, 68, 73, 78, 108
CviJI	RGCY	9 cut(s)	5, 9, 25, 41, 106, 137, 149, 180, 185
CviKI_1	RGCY	9 cut(s)	5, 9, 25, 41, 106, 137, 149, 180, 185
DpnI	GATC	2 cut(s)	201, 314
DpnII	GATC	2 cut(s)	199, 312
EaeI	YGGCCR	1 cut(s)	183
EciI	GGCGGA	1 cut(s)	147
Eco130I	CCWWGG	1 cut(s)	100
Eco47I	GGWCC	1 cut(s)	189
Eco57I	CTGAAG	1 cut(s)	245
EcoRII	CCWGG	1 cut(s)	185
EcoT14I	CCWWGG	1 cut(s)	100
EcoT22I	ATGCAT	1 cut(s)	113
ErhI	CCWWGG	1 cut(s)	100
FaeI	CATG	5 cut(s)	52, 71, 76, 81, 111
FaiI	YATR	5 cut(s)	50, 69, 74, 79, 109
FaqI	GGGAC	2 cut(s)	125, 287
FatI	CATG	5 cut(s)	48, 67, 72, 77, 107
Fnu4HI	GCNGC	3 cut(s)	154, 270, 273
Fsp4HI	GCNGC	3 cut(s)	154, 270, 273
FspBI	CTAG	2 cut(s)	6, 93
GlaI	GCGC	1 cut(s)	334
GluI	GCNGC	3 cut(s)	154, 270, 273
GsuI	CTGGAG	1 cut(s)	129
HaeIII	GGCC	3 cut(s)	41, 106, 185
HapII	CCGG	1 cut(s)	237
HhaI	GCGC	1 cut(s)	335
Hin1II	CATG	5 cut(s)	52, 71, 76, 81, 111
Hin6I	GCGC	1 cut(s)	333
HinP1I	GCGC	1 cut(s)	333
HincII	GTYRAC	2 cut(s)	256, 298
HindII	GTYRAC	2 cut(s)	256, 298
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	3 cut(s)	162, 229, 294
HpaI	GTTAAC	1 cut(s)	256
HpaII	CCGG	1 cut(s)	237
HphI	GGTGA	1 cut(s)	67
Hpy166II	GTNNAC	3 cut(s)	83, 256, 298
Hpy188I	TCNGA	1 cut(s)	167
Hpy188III	TCNNGA	1 cut(s)	310
Hpy8I	GTNNAC	3 cut(s)	83, 256, 298
HpyAV	CCTTC	1 cut(s)	316
HpyCH4IV	ACGT	3 cut(s)	120, 300, 338
HpyCH4V	TGCA	6 cut(s)	14, 48, 111, 156, 216, 269
HpyF10VI	GCNNNNNNNGC	1 cut(s)	11
HpySE526I	ACGT	3 cut(s)	120, 300, 338
Hsp92II	CATG	5 cut(s)	52, 71, 76, 81, 111
HspAI	GCGC	1 cut(s)	333
KspAI	GTTAAC	1 cut(s)	256
Kzo9I	GATC	2 cut(s)	199, 312
LmnI	GCTCC	1 cut(s)	134
LpnPI	CCDG	8 cut(s)	159, 172, 199, 218, 226, 236, 250, 294
Lsp1109I	GCAGC	3 cut(s)	140, 281, 284
LweI	GCATC	1 cut(s)	252
MaeI	CTAG	2 cut(s)	6, 93
MaeII	ACGT	3 cut(s)	120, 300, 338
MaeIII	GTNAC	2 cut(s)	16, 88
MalI	GATC	2 cut(s)	201, 314
MboI	GATC	2 cut(s)	199, 312
MboII	GAAGA	1 cut(s)	238
MlsI	TGGCCA	1 cut(s)	185
MluNI	TGGCCA	1 cut(s)	185
MlyI	GAGTC	1 cut(s)	303
MnlI	CCTC	3 cut(s)	117, 153, 331
Mox20I	TGGCCA	1 cut(s)	185
Mph1103I	ATGCAT	1 cut(s)	113
MscI	TGGCCA	1 cut(s)	185
MseI	TTAA	3 cut(s)	114, 174, 255
MslI	CAYNNNNRTG	1 cut(s)	53
Msp20I	TGGCCA	1 cut(s)	185
MspA1I	CMGCKG	1 cut(s)	197
MspI	CCGG	1 cut(s)	237
MspR9I	CCNGG	1 cut(s)	187
MvaI	CCWGG	1 cut(s)	187
MwoI	GCNNNNNNNGC	1 cut(s)	11
NdeII	GATC	2 cut(s)	199, 312
NheI	GCTAGC	1 cut(s)	5
NlaIII	CATG	5 cut(s)	52, 71, 76, 81, 111
NmuCI	GTSAC	2 cut(s)	16, 88
NsiI	ATGCAT	1 cut(s)	113
NspI	RCATGY	2 cut(s)	52, 81
PciI	ACATGT	1 cut(s)	77
PfeI	GAWTC	2 cut(s)	162, 229
PkrI	GCNGC	3 cut(s)	155, 271, 274
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Ppu21I	YACGTR	2 cut(s)	121, 339
PscI	ACATGT	1 cut(s)	77
Psp6I	CCWGG	1 cut(s)	185
PspGI	CCWGG	1 cut(s)	185
PspPI	GGNCC	2 cut(s)	104, 189
PstI	CTGCAG	1 cut(s)	16
RseI	CAYNNNNRTG	1 cut(s)	53
SaqAI	TTAA	3 cut(s)	114, 174, 255
SatI	GCNGC	3 cut(s)	154, 270, 273
Sau3AI	GATC	2 cut(s)	199, 312
Sau96I	GGNCC	2 cut(s)	104, 189
SchI	GAGTC	1 cut(s)	303
ScrFI	CCNGG	1 cut(s)	187
SfaNI	GCATC	1 cut(s)	252
SfcI	CTRYAG	1 cut(s)	12
SinI	GGWCC	1 cut(s)	189
SmiMI	CAYNNNNRTG	1 cut(s)	53
SsiI	CCGC	2 cut(s)	132, 195
SspMI	CTAG	2 cut(s)	6, 93
StyD4I	CCNGG	1 cut(s)	185
StyI	CCWWGG	1 cut(s)	100
TaiI	ACGT	3 cut(s)	123, 303, 341
TfiI	GAWTC	2 cut(s)	162, 229
Tru1I	TTAA	3 cut(s)	114, 174, 255
Tru9I	TTAA	3 cut(s)	114, 174, 255
TscAI	CASTG	2 cut(s)	21, 253
TseFI	GTSAC	2 cut(s)	16, 88
TseI	GCWGC	3 cut(s)	153, 269, 272
Tsp45I	GTSAC	2 cut(s)	16, 88
TspDTI	ATGAA	1 cut(s)	56
TspRI	CASTG	2 cut(s)	21, 253
VpaK11BI	GGWCC	1 cut(s)	189
XceI	RCATGY	2 cut(s)	52, 81
XcmI	CCANNNNNNNNNTGG	2 cut(s)	28, 178
XspI	CTAG	2 cut(s)	6, 93
Zsp2I	ATGCAT	1 cut(s)	113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.