Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 2620 |
| Acc65I | GGTACC | 1 cut(s) | 2840 |
| AccB1I | GGYRCC | 2 cut(s) | 1486, 2840 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1835 |
| AccBSI | CCGCTC | 1 cut(s) | 292 |
| AccI | GTMKAC | 1 cut(s) | 146 |
| AccII | CGCG | 2 cut(s) | 192, 1441 |
| AccIII | TCCGGA | 1 cut(s) | 162 |
| AciI | CCGC | 7 cut(s) | 14, 17, 206, 292, 1441, 1498, 2480 |
| AclWI | GGATC | 5 cut(s) | 195, 987, 1846, 2065, 2852 |
| AcoI | YGGCCR | 2 cut(s) | 1545, 2923 |
| AcsI | RAATTY | 3 cut(s) | 1729, 2510, 2772 |
| AcuI | CTGAAG | 7 cut(s) | 122, 254, 791, 1097, 1113, 2547, 2841 |
| AcyI | GRCGYC | 1 cut(s) | 1857 |
| AfaI | GTAC | 6 cut(s) | 1005, 1099, 2172, 2355, 2842, 2874 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 61, 175, 916, 1000, 1490, 1835, 2341, 2837, 2867 |
| AhlI | ACTAGT | 1 cut(s) | 2095 |
| AjnI | CCWGG | 4 cut(s) | 1467, 1489, 2339, 2920 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1133, 1165 |
| AleI | CACNNNNGTG | 2 cut(s) | 1618, 2567 |
| Alw21I | GWGCWC | 1 cut(s) | 162 |
| Alw26I | GTCTC | 2 cut(s) | 1625, 1828 |
| AlwI | GGATC | 5 cut(s) | 195, 987, 1846, 2065, 2852 |
| Ama87I | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| Aor13HI | TCCGGA | 1 cut(s) | 162 |
| AoxI | GGCC | 5 cut(s) | 1465, 1470, 1545, 2923, 2932 |
| ApoI | RAATTY | 3 cut(s) | 1729, 2510, 2772 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 758, 1087 |
| Asp718I | GGTACC | 1 cut(s) | 2840 |
| AspA2I | CCTAGG | 1 cut(s) | 55 |
| AspLEI | GCGC | 1 cut(s) | 1757 |
| AspS9I | GGNCC | 5 cut(s) | 133, 165, 1471, 1696, 2933 |
| AsuC2I | CCSGG | 6 cut(s) | 151, 169, 1018, 1019, 1136, 1137 |
| AvaI | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| AvaII | GGWCC | 3 cut(s) | 133, 165, 1696 |
| AvrII | CCTAGG | 1 cut(s) | 55 |
| BanI | GGYRCC | 2 cut(s) | 1486, 2840 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1641, 1673, 2865, 2897 |
| BbsI | GAAGAC | 2 cut(s) | 1119, 1860 |
| Bbv12I | GWGCWC | 1 cut(s) | 162 |
| BccI | CCATC | 7 cut(s) | 205, 232, 634, 825, 1034, 2523, 2596 |
| BceAI | ACGGC | 4 cut(s) | 27, 78, 1560, 2910 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 892, 926 |
| BciT130I | CCWGG | 4 cut(s) | 1469, 1491, 2341, 2922 |
| BcnI | CCSGG | 6 cut(s) | 151, 169, 1018, 1019, 1136, 1137 |
| BcoDI | GTCTC | 2 cut(s) | 1625, 1828 |
| BcuI | ACTAGT | 1 cut(s) | 2095 |
| BfaI | CTAG | 8 cut(s) | 56, 468, 965, 1868, 2096, 2424, 2655, 2736 |
| BfmI | CTRYAG | 2 cut(s) | 2034, 2576 |
| BfuAI | ACCTGC | 1 cut(s) | 2620 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2931 |
| BglII | AGATCT | 2 cut(s) | 2669, 2750 |
| BlnI | CCTAGG | 1 cut(s) | 55 |
| Bme18I | GGWCC | 3 cut(s) | 133, 165, 1696 |
| BmeT110I | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| BmgT120I | GGNCC | 5 cut(s) | 133, 165, 1471, 1696, 2933 |
| BmiI | GGNNCC | 6 cut(s) | 53, 167, 1488, 1530, 1698, 2842 |
| BpiI | GAAGAC | 2 cut(s) | 1119, 1860 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1240, 1272 |
| BpmI | CTGGAG | 3 cut(s) | 149, 977, 2151 |
| Bpu10I | CCTNAGC | 1 cut(s) | 371 |
| BpuEI | CTTGAG | 3 cut(s) | 1257, 1827, 2027 |
| BpuMI | CCSGG | 6 cut(s) | 151, 169, 1018, 1019, 1136, 1137 |
| Bsa29I | ATCGAT | 1 cut(s) | 1086 |
| BsaAI | YACGTR | 1 cut(s) | 2153 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2674, 2755 |
| BsaHI | GRCGYC | 1 cut(s) | 1857 |
| BsaWI | WCCGGW | 1 cut(s) | 162 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 248, 278, 374, 404, 521, 551, 776, 806 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 61, 175, 916, 1000, 1490, 1835, 2341, 2837, 2867 |
| Bse1I | ACTGG | 3 cut(s) | 1302, 1751, 2168 |
| Bse3DI | GCAATG | 2 cut(s) | 1164, 2208 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2674, 2755 |
| BseAI | TCCGGA | 1 cut(s) | 162 |
| BseBI | CCWGG | 4 cut(s) | 1469, 1491, 2341, 2922 |
| BseCI | ATCGAT | 1 cut(s) | 1086 |
| BseGI | GGATG | 5 cut(s) | 836, 1045, 1159, 2203, 2897 |
| BseJI | GATNNNNATC | 2 cut(s) | 2674, 2755 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 61, 175, 916, 1000, 1490, 1835, 2341, 2837, 2867 |
| BseMI | GCAATG | 2 cut(s) | 1164, 2208 |
| BseMII | CTCAG | 1 cut(s) | 689 |
| BseNI | ACTGG | 3 cut(s) | 1302, 1751, 2168 |
| Bsh1236I | CGCG | 2 cut(s) | 192, 1441 |
| BshFI | GGCC | 5 cut(s) | 1467, 1472, 1547, 2925, 2934 |
| BshNI | GGYRCC | 2 cut(s) | 1486, 2840 |
| BshVI | ATCGAT | 1 cut(s) | 1086 |
| BsiHKAI | GWGCWC | 1 cut(s) | 162 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| BsiSI | CCGG | 6 cut(s) | 151, 163, 169, 1018, 1136, 1484 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 61, 175, 916, 1000, 1490, 1835, 2341, 2837, 2867 |
| BsmAI | GTCTC | 2 cut(s) | 1625, 1828 |
| BsmI | GAATGC | 1 cut(s) | 985 |
| BsnI | GGCC | 5 cut(s) | 1467, 1472, 1547, 2925, 2934 |
| BsoBI | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| Bsp1286I | GDGCHC | 1 cut(s) | 162 |
| Bsp13I | TCCGGA | 1 cut(s) | 162 |
| Bsp1407I | TGTACA | 1 cut(s) | 2872 |
| Bsp143I | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| Bsp19I | CCATGG | 2 cut(s) | 1092, 2901 |
| BspACI | CCGC | 7 cut(s) | 14, 17, 206, 292, 1441, 1498, 2480 |
| BspANI | GGCC | 5 cut(s) | 1467, 1472, 1547, 2925, 2934 |
| BspCNI | CTCAG | 1 cut(s) | 690 |
| BspDI | ATCGAT | 1 cut(s) | 1086 |
| BspEI | TCCGGA | 1 cut(s) | 162 |
| BspFNI | CGCG | 2 cut(s) | 192, 1441 |
| BspLI | GGNNCC | 6 cut(s) | 53, 167, 1488, 1530, 1698, 2842 |
| BspMAI | CTGCAG | 1 cut(s) | 2580 |
| BspMI | ACCTGC | 1 cut(s) | 2620 |
| BspPI | GGATC | 5 cut(s) | 195, 987, 1846, 2065, 2852 |
| BspT107I | GGYRCC | 2 cut(s) | 1486, 2840 |
| BsrBI | CCGCTC | 1 cut(s) | 292 |
| BsrDI | GCAATG | 2 cut(s) | 1164, 2208 |
| BsrGI | TGTACA | 1 cut(s) | 2872 |
| BsrI | ACTGG | 3 cut(s) | 1302, 1751, 2168 |
| BssMI | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| BssNI | GRCGYC | 1 cut(s) | 1857 |
| BssT1I | CCWWGG | 4 cut(s) | 55, 1092, 1474, 2901 |
| Bst2UI | CCWGG | 4 cut(s) | 1469, 1491, 2341, 2922 |
| Bst4CI | ACNGT | 4 cut(s) | 112, 1107, 1327, 2293 |
| Bst6I | CTCTTC | 5 cut(s) | 290, 596, 1039, 1073, 2201 |
| BstACI | GRCGYC | 1 cut(s) | 1857 |
| BstAUI | TGTACA | 1 cut(s) | 2872 |
| BstBAI | YACGTR | 1 cut(s) | 2153 |
| BstC8I | GCNNGC | 1 cut(s) | 87 |
| BstDEI | CTNAG | 3 cut(s) | 371, 698, 2815 |
| BstDSI | CCRYGG | 3 cut(s) | 1092, 1542, 2901 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 914 |
| BstF5I | GGATG | 5 cut(s) | 836, 1045, 1159, 2203, 2897 |
| BstFNI | CGCG | 2 cut(s) | 192, 1441 |
| BstHHI | GCGC | 1 cut(s) | 1757 |
| BstKTI | GATC | 8 cut(s) | 190, 982, 1032, 1841, 2073, 2672, 2753, 2860 |
| BstMAI | GTCTC | 2 cut(s) | 1625, 1828 |
| BstMBI | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1420, 1553, 2531, 2816, 2931 |
| BstNI | CCWGG | 4 cut(s) | 1469, 1491, 2341, 2922 |
| BstNSI | RCATGY | 1 cut(s) | 455 |
| BstSFI | CTRYAG | 2 cut(s) | 2034, 2576 |
| BstUI | CGCG | 2 cut(s) | 192, 1441 |
| BstV2I | GAAGAC | 2 cut(s) | 1119, 1860 |
| BstX2I | RGATCY | 5 cut(s) | 979, 1838, 2070, 2669, 2750 |
| BstYI | RGATCY | 5 cut(s) | 979, 1838, 2070, 2669, 2750 |
| Bsu15I | ATCGAT | 1 cut(s) | 1086 |
| BsuRI | GGCC | 5 cut(s) | 1467, 1472, 1547, 2925, 2934 |
| BsuTUI | ATCGAT | 1 cut(s) | 1086 |
| BtgI | CCRYGG | 3 cut(s) | 1092, 1542, 2901 |
| BtsCI | GGATG | 5 cut(s) | 836, 1045, 1159, 2203, 2897 |
| BtsIMutI | CAGTG | 1 cut(s) | 1332 |
| BveI | ACCTGC | 1 cut(s) | 2620 |
| Cac8I | GCNNGC | 1 cut(s) | 87 |
| CfoI | GCGC | 1 cut(s) | 1757 |
| Cfr13I | GGNCC | 5 cut(s) | 133, 165, 1471, 1696, 2933 |
| Cfr9I | CCCGGG | 2 cut(s) | 1017, 1135 |
| ClaI | ATCGAT | 1 cut(s) | 1086 |
| CseI | GACGC | 2 cut(s) | 1295, 1865 |
| Csp6I | GTAC | 6 cut(s) | 1004, 1098, 2171, 2354, 2841, 2873 |
| CviQI | GTAC | 6 cut(s) | 1004, 1098, 2171, 2354, 2841, 2873 |
| DdeI | CTNAG | 3 cut(s) | 371, 698, 2815 |
| DpnI | GATC | 8 cut(s) | 189, 981, 1031, 1840, 2072, 2671, 2752, 2859 |
| DpnII | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| EaeI | YGGCCR | 2 cut(s) | 1545, 2923 |
| Eam1104I | CTCTTC | 5 cut(s) | 290, 596, 1039, 1073, 2201 |
| EarI | CTCTTC | 5 cut(s) | 290, 596, 1039, 1073, 2201 |
| EciI | GGCGGA | 2 cut(s) | 32, 221 |
| Eco130I | CCWWGG | 4 cut(s) | 55, 1092, 1474, 2901 |
| Eco147I | AGGCCT | 1 cut(s) | 1467 |
| Eco47I | GGWCC | 3 cut(s) | 133, 165, 1696 |
| Eco57I | CTGAAG | 7 cut(s) | 122, 254, 791, 1097, 1113, 2547, 2841 |
| Eco88I | CYCGRG | 3 cut(s) | 1017, 1135, 2321 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 914 |
| EcoRII | CCWGG | 4 cut(s) | 1467, 1489, 2339, 2920 |
| EcoT14I | CCWWGG | 4 cut(s) | 55, 1092, 1474, 2901 |
| EcoT22I | ATGCAT | 2 cut(s) | 2055, 2306 |
| ErhI | CCWWGG | 4 cut(s) | 55, 1092, 1474, 2901 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2099, 2131 |
| FauI | CCCGC | 1 cut(s) | 1434 |
| FauNDI | CATATG | 2 cut(s) | 1960, 2686 |
| FblI | GTMKAC | 1 cut(s) | 146 |
| FokI | GGATG | 5 cut(s) | 843, 1052, 1166, 2210, 2884 |
| FspBI | CTAG | 8 cut(s) | 56, 468, 965, 1868, 2096, 2424, 2655, 2736 |
| GlaI | GCGC | 1 cut(s) | 1756 |
| GsuI | CTGGAG | 3 cut(s) | 149, 977, 2151 |
| HaeIII | GGCC | 5 cut(s) | 1467, 1472, 1547, 2925, 2934 |
| HapII | CCGG | 6 cut(s) | 151, 163, 169, 1018, 1136, 1484 |
| HgaI | GACGC | 2 cut(s) | 1295, 1865 |
| HhaI | GCGC | 1 cut(s) | 1757 |
| Hin1I | GRCGYC | 1 cut(s) | 1857 |
| Hin6I | GCGC | 1 cut(s) | 1755 |
| HinP1I | GCGC | 1 cut(s) | 1755 |
| HincII | GTYRAC | 1 cut(s) | 147 |
| HindII | GTYRAC | 1 cut(s) | 147 |
| HindIII | AAGCTT | 2 cut(s) | 87, 2012 |
| HpaII | CCGG | 6 cut(s) | 151, 163, 169, 1018, 1136, 1484 |
| Hpy166II | GTNNAC | 5 cut(s) | 108, 147, 1448, 2163, 2873 |
| Hpy188III | TCNNGA | 5 cut(s) | 163, 994, 2006, 2063, 2583 |
| Hpy8I | GTNNAC | 5 cut(s) | 108, 147, 1448, 2163, 2873 |
| Hpy99I | CGWCG | 1 cut(s) | 97 |
| HpyCH4III | ACNGT | 4 cut(s) | 112, 1107, 1327, 2293 |
| HpyCH4IV | ACGT | 2 cut(s) | 2152, 2455 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1420, 1553, 2531, 2816, 2931 |
| HpyF3I | CTNAG | 3 cut(s) | 371, 698, 2815 |
| HpySE526I | ACGT | 2 cut(s) | 2152, 2455 |
| Hsp92I | GRCGYC | 1 cut(s) | 1857 |
| HspAI | GCGC | 1 cut(s) | 1755 |
| Kpn2I | TCCGGA | 1 cut(s) | 162 |
| KpnI | GGTACC | 1 cut(s) | 2844 |
| Kzo9I | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| LmnI | GCTCC | 8 cut(s) | 51, 124, 165, 289, 367, 820, 883, 1411 |
| MaeI | CTAG | 8 cut(s) | 56, 468, 965, 1868, 2096, 2424, 2655, 2736 |
| MaeII | ACGT | 2 cut(s) | 2152, 2455 |
| MaeIII | GTNAC | 5 cut(s) | 960, 1334, 2148, 2153, 2419 |
| MalI | GATC | 8 cut(s) | 189, 981, 1031, 1840, 2072, 2671, 2752, 2859 |
| MbiI | CCGCTC | 1 cut(s) | 292 |
| MboI | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| MflI | RGATCY | 5 cut(s) | 979, 1838, 2070, 2669, 2750 |
| MhlI | GDGCHC | 1 cut(s) | 162 |
| MlyI | GAGTC | 9 cut(s) | 80, 152, 341, 1133, 1830, 1866, 2184, 2333, 2449 |
| MmeI | TCCRAC | 3 cut(s) | 825, 1326, 1816 |
| Mph1103I | ATGCAT | 2 cut(s) | 2055, 2306 |
| MroI | TCCGGA | 1 cut(s) | 162 |
| MroXI | GAANNNNTTC | 2 cut(s) | 758, 1087 |
| MseI | TTAA | 9 cut(s) | 810, 1119, 1388, 1416, 1805, 2139, 2180, 2826, 2941 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1618, 2567, 2868 |
| MspA1I | CMGCKG | 1 cut(s) | 2525 |
| MspI | CCGG | 6 cut(s) | 151, 163, 169, 1018, 1136, 1484 |
| Mva1269I | GAATGC | 1 cut(s) | 985 |
| MvaI | CCWGG | 4 cut(s) | 1469, 1491, 2341, 2922 |
| MvnI | CGCG | 2 cut(s) | 192, 1441 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1420, 1553, 2531, 2816, 2931 |
| NciI | CCSGG | 6 cut(s) | 151, 169, 1018, 1019, 1136, 1137 |
| NcoI | CCATGG | 2 cut(s) | 1092, 2901 |
| NdeI | CATATG | 2 cut(s) | 1960, 2686 |
| NdeII | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| NlaIV | GGNNCC | 6 cut(s) | 53, 167, 1488, 1530, 1698, 2842 |
| NmuCI | GTSAC | 3 cut(s) | 1334, 2153, 2419 |
| NsiI | ATGCAT | 2 cut(s) | 2055, 2306 |
| NspI | RCATGY | 1 cut(s) | 455 |
| OliI | CACNNNNGTG | 2 cut(s) | 1618, 2567 |
| PaeR7I | CTCGAG | 1 cut(s) | 2321 |
| PceI | AGGCCT | 1 cut(s) | 1467 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 89 |
| PctI | GAATGC | 1 cut(s) | 985 |
| PdmI | GAANNNNTTC | 2 cut(s) | 758, 1087 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1835 |
| PleI | GAGTC | 9 cut(s) | 79, 151, 340, 1132, 1829, 1866, 2183, 2332, 2448 |
| PpsI | GAGTC | 9 cut(s) | 79, 151, 340, 1132, 1829, 1866, 2183, 2332, 2448 |
| Ppu21I | YACGTR | 1 cut(s) | 2153 |
| Psp6I | CCWGG | 4 cut(s) | 1467, 1489, 2339, 2920 |
| PspGI | CCWGG | 4 cut(s) | 1467, 1489, 2339, 2920 |
| PspN4I | GGNNCC | 6 cut(s) | 53, 167, 1488, 1530, 1698, 2842 |
| PspPI | GGNCC | 5 cut(s) | 133, 165, 1471, 1696, 2933 |
| PspXI | VCTCGAGB | 1 cut(s) | 2321 |
| PstI | CTGCAG | 1 cut(s) | 2580 |
| PsuI | RGATCY | 5 cut(s) | 979, 1838, 2070, 2669, 2750 |
| PvuII | CAGCTG | 1 cut(s) | 2525 |
| RsaI | GTAC | 6 cut(s) | 1005, 1099, 2172, 2355, 2842, 2874 |
| RsaNI | GTAC | 6 cut(s) | 1004, 1098, 2171, 2354, 2841, 2873 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1618, 2567, 2868 |
| SalI | GTCGAC | 1 cut(s) | 145 |
| SaqAI | TTAA | 9 cut(s) | 810, 1119, 1388, 1416, 1805, 2139, 2180, 2826, 2941 |
| Sau3AI | GATC | 8 cut(s) | 187, 979, 1029, 1838, 2070, 2669, 2750, 2857 |
| Sau96I | GGNCC | 5 cut(s) | 133, 165, 1471, 1696, 2933 |
| SchI | GAGTC | 9 cut(s) | 80, 152, 341, 1133, 1830, 1866, 2184, 2333, 2449 |
| SduI | GDGCHC | 1 cut(s) | 162 |
| SfcI | CTRYAG | 2 cut(s) | 2034, 2576 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2931 |
| Sfr274I | CTCGAG | 1 cut(s) | 2321 |
| SinI | GGWCC | 3 cut(s) | 133, 165, 1696 |
| SlaI | CTCGAG | 1 cut(s) | 2321 |
| SmaI | CCCGGG | 2 cut(s) | 1019, 1137 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1618, 2567, 2868 |
| SmlI | CTYRAG | 4 cut(s) | 1272, 1842, 2006, 2321 |
| SmoI | CTYRAG | 4 cut(s) | 1272, 1842, 2006, 2321 |
| SpeI | ACTAGT | 1 cut(s) | 2095 |
| SseBI | AGGCCT | 1 cut(s) | 1467 |
| SsiI | CCGC | 7 cut(s) | 14, 17, 206, 292, 1441, 1498, 2480 |
| SspI | AATATT | 1 cut(s) | 1795 |
| SspMI | CTAG | 8 cut(s) | 56, 468, 965, 1868, 2096, 2424, 2655, 2736 |
| StuI | AGGCCT | 1 cut(s) | 1467 |
| StyI | CCWWGG | 4 cut(s) | 55, 1092, 1474, 2901 |
| TaaI | ACNGT | 4 cut(s) | 112, 1107, 1327, 2293 |
| TaiI | ACGT | 2 cut(s) | 2155, 2458 |
| TatI | WGTACW | 2 cut(s) | 2353, 2872 |
| TauI | GCSGC | 2 cut(s) | 17, 1500 |
| Tru1I | TTAA | 9 cut(s) | 810, 1119, 1388, 1416, 1805, 2139, 2180, 2826, 2941 |
| Tru9I | TTAA | 9 cut(s) | 810, 1119, 1388, 1416, 1805, 2139, 2180, 2826, 2941 |
| TscAI | CASTG | 1 cut(s) | 1332 |
| TseFI | GTSAC | 3 cut(s) | 1334, 2153, 2419 |
| Tsp45I | GTSAC | 3 cut(s) | 1334, 2153, 2419 |
| TspGWI | ACGGA | 1 cut(s) | 2712 |
| TspMI | CCCGGG | 2 cut(s) | 1017, 1135 |
| TspRI | CASTG | 1 cut(s) | 1332 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1835 |
| VpaK11BI | GGWCC | 3 cut(s) | 133, 165, 1696 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 914 |
| XapI | RAATTY | 3 cut(s) | 1729, 2510, 2772 |
| XceI | RCATGY | 1 cut(s) | 455 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1324 |
| XhoI | CTCGAG | 1 cut(s) | 2321 |
| XmaI | CCCGGG | 2 cut(s) | 1017, 1135 |
| XmaJI | CCTAGG | 1 cut(s) | 55 |
| XmiI | GTMKAC | 1 cut(s) | 146 |
| XmnI | GAANNNNTTC | 2 cut(s) | 758, 1087 |
| XspI | CTAG | 8 cut(s) | 56, 468, 965, 1868, 2096, 2424, 2655, 2736 |
| Zsp2I | ATGCAT | 2 cut(s) | 2055, 2306 |