Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001353

Physical Location & Seq

Genomic Coordinates Forward (+)

26965545 .. 26966281

Gene Structure 737 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1187500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 360 bp

ATGGCGAACTCTGCGGCATTCAAGCTTGCCTTCGTGGCCCTCGTGTGCATTGTGGTGGGTGCACCATTCGCCCAAGCTACCATATCATCATGCGGCCAGGTGACAAACTACGTGGCACCCTGCATTCCTTACTTGAAGACTGGTGGGAAAGTTCCACCAACATGTTGCCAAGGGATCCAGAAGCTGAACGACGCCGCTCAGACCGCACCTGATCGCCGAACCGCTTGCACCTGCTTGGTCAACACTGCTACAAAAGTCCAAGGAATAAAACCTAACCTCATCTATGGACTCCCTGGGGCCTGTGGCGTCCGCCTTCCTTACCCAATTGGACCGAACACTAAATGCAACAGTATCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001101 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006649 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009505 GO:0009506 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0009628 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010026 GO:0010035 GO:0010154 GO:0010556 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016049 GO:0019222 GO:0022412 GO:0022414 GO:0030054 GO:0030154 GO:0030312 GO:0031410 GO:0031976 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0033036 GO:0040007 GO:0042221 GO:0042335 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0060255 GO:0060560 GO:0061024 GO:0061458 GO:0065007 GO:0071702 GO:0071840 GO:0071944 GO:0090376 GO:0090378 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:1901700 GO:1901957

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

12.33

Weight (kDa)

9.2

Isoelectric Point (pI)

27.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	20 - 106	1.6e-07	Probable lipid transfer
Tryp_alpha_amyl	PF00234	31 - 115	1.5e-11	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33355 AT4G33355
fragaria_vesca	FvH4_6g09970 FvH4_6g09980
malus_domestica	MD04G1173600.v1.1 MD04G1173800.v1.1 MD12G1187100.v1.1 MD12G1187200.v1.1 MD12G1187500.v1.1
prunus_persica	Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292600_v2.0.a1 Prupe.6G292800_v2.0.a1
pyrus_communis	pycom04g15340 pycom12g17540 pycom12g17550
rosa_chinensis	RchiOBHm_Chr3g0460591 RchiOBHm_Chr3g0460641 RchiOBHm_Chr3g0460651 RchiOBHm_Chr7g0211001
rosa_laevigata	RLG00000003030 RLG00000024990 RLG00000024991
rosa_multiflora	Rmu_co8436807.1_g000001 Rmu_sc0001256.1_g000005 Rmu_sc0001256.1_g000008 Rmu_sc0001256.1_g000009 Rmu_sc0003701.1_g000008 Rmu_sc0011069.1_g000012
rosa_roxburghii	Rroxscaffold_3G00248180 Rroxscaffold_6G00419330 Rroxscaffold_6G00419340
rosa_rugosa	Rorug03G0044200 Rorug03G0044300 Rorug03G0044400 Rorug03G0044500 Rorug07G0123800
rosa_samantha	Rh3AG102300 Rh3AG102400 Rh3BG105900 Rh3BG106000 Rh3BG106100 Rh3CG107900 Rh3CG108000 Rh3DG107400 Rh3DG107600 Rh7AG255400 Rh7BG249900 Rh7CG272000
rosa_wichuraiana	Rw0G010020 Rw3G008690 Rw3G008710 Rw7G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	239
Acc36I	ACCTGC	1 cut(s)	239
AccB1I	GGYRCC	1 cut(s)	115
AccBSI	CCGCTC	1 cut(s)	197
AciI	CCGC	6 cut(s)	14, 93, 195, 204, 222, 310
AclWI	GGATC	2 cut(s)	169, 182
AcoI	YGGCCR	1 cut(s)	94
AcyI	GRCGYC	2 cut(s)	192, 306
AflIII	ACRYGT	1 cut(s)	161
AgsI	TTSAA	2 cut(s)	22, 136
AjnI	CCWGG	2 cut(s)	96, 292
AluBI	AGCT	3 cut(s)	25, 77, 184
AluI	AGCT	3 cut(s)	25, 77, 184
Alw21I	GWGCWC	1 cut(s)	64
Alw44I	GTGCAC	1 cut(s)	60
AlwI	GGATC	2 cut(s)	169, 182
AlwNI	CAGNNNCTG	1 cut(s)	184
AoxI	GGCC	3 cut(s)	36, 94, 297
ApaLI	GTGCAC	1 cut(s)	60
AspS9I	GGNCC	3 cut(s)	37, 297, 329
AsuHPI	GGTGA	1 cut(s)	112
AvaII	GGWCC	1 cut(s)	329
BaeGI	GKGCMC	1 cut(s)	64
BamHI	GGATCC	1 cut(s)	174
BanI	GGYRCC	1 cut(s)	115
BauI	CACGAG	1 cut(s)	41
BbsI	GAAGAC	1 cut(s)	143
Bbv12I	GWGCWC	1 cut(s)	64
BcgI	CGANNNNNNTGC	2 cut(s)	207, 241
BciT130I	CCWGG	2 cut(s)	98, 294
BfuAI	ACCTGC	1 cut(s)	239
BglI	GCCNNNNNGGC	1 cut(s)	35
BisI	GCNGC	3 cut(s)	15, 94, 195
BlsI	GCNGC	3 cut(s)	16, 95, 196
Bme1390I	CCNGG	2 cut(s)	98, 294
Bme18I	GGWCC	1 cut(s)	329
BmgT120I	GGNCC	3 cut(s)	37, 297, 329
BmiI	GGNNCC	3 cut(s)	117, 176, 298
BmrFI	CCNGG	2 cut(s)	98, 294
BpiI	GAAGAC	1 cut(s)	143
BsaAI	YACGTR	1 cut(s)	112
BsaHI	GRCGYC	2 cut(s)	192, 306
BsaJI	CCNNGG	4 cut(s)	169, 259, 292, 293
Bse1I	ACTGG	2 cut(s)	145, 355
BseBI	CCWGG	2 cut(s)	98, 294
BseDI	CCNNGG	4 cut(s)	169, 259, 292, 293
BseMII	CTCAG	1 cut(s)	212
BseNI	ACTGG	2 cut(s)	145, 355
BseSI	GKGCMC	1 cut(s)	64
BshFI	GGCC	3 cut(s)	38, 96, 299
BshNI	GGYRCC	1 cut(s)	115
BsiHKAI	GWGCWC	1 cut(s)	64
BsmI	GAATGC	2 cut(s)	17, 123
BsnI	GGCC	3 cut(s)	38, 96, 299
Bsp1286I	GDGCHC	1 cut(s)	64
Bsp143I	GATC	2 cut(s)	174, 211
BspACI	CCGC	6 cut(s)	14, 93, 195, 204, 222, 310
BspANI	GGCC	3 cut(s)	38, 96, 299
BspCNI	CTCAG	1 cut(s)	211
BspLI	GGNNCC	3 cut(s)	117, 176, 298
BspMI	ACCTGC	1 cut(s)	239
BspPI	GGATC	2 cut(s)	169, 182
BspT107I	GGYRCC	1 cut(s)	115
BsrBI	CCGCTC	1 cut(s)	197
BsrI	ACTGG	2 cut(s)	145, 355
BssECI	CCNNGG	4 cut(s)	169, 259, 292, 293
BssMI	GATC	2 cut(s)	174, 211
BssNI	GRCGYC	2 cut(s)	192, 306
BssSI	CACGAG	1 cut(s)	41
BssT1I	CCWWGG	2 cut(s)	169, 259
Bst2BI	CACGAG	1 cut(s)	41
Bst2UI	CCWGG	2 cut(s)	98, 294
Bst4CI	ACNGT	1 cut(s)	350
BstACI	GRCGYC	2 cut(s)	192, 306
BstBAI	YACGTR	1 cut(s)	112
BstC8I	GCNNGC	2 cut(s)	27, 226
BstDEI	CTNAG	1 cut(s)	198
BstKTI	GATC	2 cut(s)	177, 214
BstMBI	GATC	2 cut(s)	174, 211
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 35, 68, 203
BstNI	CCWGG	2 cut(s)	98, 294
BstNSI	RCATGY	1 cut(s)	165
BstSCI	CCNGG	2 cut(s)	96, 292
BstSLI	GKGCMC	1 cut(s)	64
BstV2I	GAAGAC	1 cut(s)	143
BstX2I	RGATCY	1 cut(s)	174
BstYI	RGATCY	1 cut(s)	174
BsuRI	GGCC	3 cut(s)	38, 96, 299
BtsI	GCAGTG	1 cut(s)	243
BtsIMutI	CAGTG	1 cut(s)	243
BveI	ACCTGC	1 cut(s)	239
Cac8I	GCNNGC	2 cut(s)	27, 226
CaiI	CAGNNNCTG	1 cut(s)	184
Cfr13I	GGNCC	3 cut(s)	37, 297, 329
CseI	GACGC	2 cut(s)	200, 295
CspCI	CAANNNNNGTGG	2 cut(s)	93, 128
CviAII	CATG	2 cut(s)	90, 162
CviJI	RGCY	6 cut(s)	25, 38, 77, 96, 184, 299
CviKI_1	RGCY	6 cut(s)	25, 38, 77, 96, 184, 299
DdeI	CTNAG	1 cut(s)	198
DpnI	GATC	2 cut(s)	176, 213
DpnII	GATC	2 cut(s)	174, 211
EaeI	YGGCCR	1 cut(s)	94
EciI	GGCGGA	1 cut(s)	299
Eco130I	CCWWGG	2 cut(s)	169, 259
Eco47I	GGWCC	1 cut(s)	329
EcoO109I	RGGNCCY	1 cut(s)	297
EcoRII	CCWGG	2 cut(s)	96, 292
EcoT14I	CCWWGG	2 cut(s)	169, 259
ErhI	CCWWGG	2 cut(s)	169, 259
FaeI	CATG	2 cut(s)	93, 165
FaiI	YATR	4 cut(s)	83, 91, 163, 285
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FatI	CATG	2 cut(s)	89, 161
Fnu4HI	GCNGC	3 cut(s)	15, 94, 195
Fsp4HI	GCNGC	3 cut(s)	15, 94, 195
GluI	GCNGC	3 cut(s)	15, 94, 195
HaeIII	GGCC	3 cut(s)	38, 96, 299
HgaI	GACGC	2 cut(s)	200, 295
Hin1I	GRCGYC	2 cut(s)	192, 306
Hin1II	CATG	2 cut(s)	93, 165
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	1 cut(s)	288
HphI	GGTGA	1 cut(s)	112
Hpy166II	GTNNAC	2 cut(s)	62, 241
Hpy188I	TCNGA	1 cut(s)	201
Hpy188III	TCNNGA	1 cut(s)	178
Hpy8I	GTNNAC	2 cut(s)	62, 241
Hpy99I	CGWCG	1 cut(s)	194
HpyAV	CCTTC	2 cut(s)	40, 323
HpyCH4III	ACNGT	1 cut(s)	350
HpyCH4IV	ACGT	1 cut(s)	111
HpyCH4V	TGCA	5 cut(s)	48, 62, 123, 228, 345
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 35, 68, 203
HpyF3I	CTNAG	1 cut(s)	198
HpySE526I	ACGT	1 cut(s)	111
Hsp92I	GRCGYC	2 cut(s)	192, 306
Hsp92II	CATG	2 cut(s)	93, 165
Kzo9I	GATC	2 cut(s)	174, 211
MaeII	ACGT	1 cut(s)	111
MaeIII	GTNAC	1 cut(s)	100
MalI	GATC	2 cut(s)	176, 213
MbiI	CCGCTC	1 cut(s)	197
MboI	GATC	2 cut(s)	174, 211
MboII	GAAGA	1 cut(s)	148
MfeI	CAATTG	1 cut(s)	324
MflI	RGATCY	1 cut(s)	174
MhlI	GDGCHC	1 cut(s)	64
MluCI	AATT	1 cut(s)	324
MlyI	GAGTC	1 cut(s)	282
MnlI	CCTC	2 cut(s)	50, 287
MslI	CAYNNNNRTG	2 cut(s)	53, 160
MspR9I	CCNGG	2 cut(s)	98, 294
MunI	CAATTG	1 cut(s)	324
Mva1269I	GAATGC	2 cut(s)	17, 123
MvaI	CCWGG	2 cut(s)	98, 294
MwoI	GCNNNNNNNGC	4 cut(s)	11, 35, 68, 203
NdeII	GATC	2 cut(s)	174, 211
NlaIII	CATG	2 cut(s)	93, 165
NlaIV	GGNNCC	3 cut(s)	117, 176, 298
NmuCI	GTSAC	1 cut(s)	100
NspI	RCATGY	1 cut(s)	165
PaqCI	CACCTGC	1 cut(s)	239
PasI	CCCWGGG	1 cut(s)	293
PciI	ACATGT	1 cut(s)	161
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	2 cut(s)	17, 123
PkrI	GCNGC	3 cut(s)	16, 95, 196
PleI	GAGTC	1 cut(s)	282
PpsI	GAGTC	1 cut(s)	282
Ppu21I	YACGTR	1 cut(s)	112
PscI	ACATGT	1 cut(s)	161
Psp6I	CCWGG	2 cut(s)	96, 292
PspGI	CCWGG	2 cut(s)	96, 292
PspN4I	GGNNCC	3 cut(s)	117, 176, 298
PspPI	GGNCC	3 cut(s)	37, 297, 329
PstNI	CAGNNNCTG	1 cut(s)	184
PsuI	RGATCY	1 cut(s)	174
RseI	CAYNNNNRTG	2 cut(s)	53, 160
SatI	GCNGC	3 cut(s)	15, 94, 195
Sau3AI	GATC	2 cut(s)	174, 211
Sau96I	GGNCC	3 cut(s)	37, 297, 329
SchI	GAGTC	1 cut(s)	282
ScrFI	CCNGG	2 cut(s)	98, 294
SduI	GDGCHC	1 cut(s)	64
SetI	ASST	9 cut(s)	27, 79, 102, 114, 186, 211, 233, 274, 279
SinI	GGWCC	1 cut(s)	329
SmiMI	CAYNNNNRTG	2 cut(s)	53, 160
Sse9I	AATT	1 cut(s)	324
SsiI	CCGC	6 cut(s)	14, 93, 195, 204, 222, 310
StyD4I	CCNGG	2 cut(s)	96, 292
StyI	CCWWGG	2 cut(s)	169, 259
TaaI	ACNGT	1 cut(s)	350
TaiI	ACGT	1 cut(s)	114
TaqII	GACCGA	1 cut(s)	346
TasI	AATT	1 cut(s)	324
TauI	GCSGC	3 cut(s)	17, 96, 197
TscAI	CASTG	1 cut(s)	250
TseFI	GTSAC	1 cut(s)	100
Tsp45I	GTSAC	1 cut(s)	100
TspRI	CASTG	1 cut(s)	250
VneI	GTGCAC	1 cut(s)	60
VpaK11BI	GGWCC	1 cut(s)	329
XceI	RCATGY	1 cut(s)	165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.