MD04G1238300.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009726
Physical Location & Seq
Forward (+)
31631387 .. 31635175
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1238300.v1.1.491

Sequence Viewer

Length: 2349 bp
ATGTCACGTTTCTTCAATAATTTGTGGAAGAAAGGTGCACTATATTCGTCTGATCATAGAAGAAAACCTACAGATACAGATAGAGATGACGCCAAATGGGCGTTTCCAGAAGAATTGTGTCGTCGGTTTTCACTCGCGGAGATCATAGCAGCAACTCAAAACTTCAATGCTAGGTTACGGGTTGGCAATACCAGAAACTTGTCATTTGACGTTAATGGCCTAATAGAAAAGTTTTTGGTGTTTGATCACCCTGAAGGTCCGAAAACAGAAGACGGTATTAATGTTAATGCTGCAGGTGTCTACAAAGGATACACCAACTTCAACGGGGTAACTTCCACGGTAGCAATCAAGCACGTGCCCGGCGCACAATCAGATATTCAGCGGGGACGCGAGTTCAAGGCGGAGGTGCAGCTACTGTGCCAGCTGCGCCACCCTAATTTAATCTCTCTAATCGGATTCTGCCAAGAAAAGGGAGAGTGCGCCATTGTTTACAACTACATGTCCAATGGTTCCCTTTCTAATTCCCTTTTCAATCCTCATAAAAATAATAAGGATCCGTTGTCTTGGAGGCAAAGGCTGAATATCTGCATTGGGGTGGCGCGTGCAATACACTATCTCCACACAGGGGTTAAGCATGCGGTAATCCACCGAGACATTAAATGTGACAACATACTCTTGGACCAAAGCTTAGAGGCCAAGCTTTCTAACTTCTGGCTGTCCAAGATGGGTACATTCGGGTATCTTGATCCAGAGTATGATTTGTCTGGCCAGCTTACGGAAAAATCTGATGTCTACTCGTTTGGAATGGTGTTATTCGAAGTGTTGTCTGCCAAATATTCAAGAGCGATACCACGAATACATATGGCTCGCCCAGCTCATATTCTAGACCCATTTCTCATGGGAAAAATAGCTCCCGATTGTTTGATAAAATTTTTGGACATCGTCCAAAGATGCGTCCGCCCTACAGGAGCCCAACGGCCCACGATGGGTGAAGTGGAGGTGGAACTTGAGCTTGCACTGGAGCTGCAGGAGAGAGCAGATGCCGGAAAGGAGTTGAGGACAACTAGCACTCCTCTTGATCAGCCTGCAGACTGTGCCTACGGTTATGAAGGGATATCAGTTTCCGAATTGAATGAGACTTTCTCCTCTCTATACATGAGTAGACAGAGTCCAGTAGTTAGCCCAACCAGGTCTGCTGATTTTTCAGTAACAACAGTTAGCACAATGATGGGTTTCAGTACTACTGCAGAGTCCGCCTCATGTTTCTTCTTCTCTGCATGGAAGAAACTAGCATCATGTGATAAAAGTAGGAAACGCAGACAAAACCAAGACTGGGCCTTTATGGAGGAATTATCCCGCCGATTTTCGCTAGCTGAGATCATAGCAGCCACTGAATGCTTCAACGACAAGTTCCATATTGGTAGCAGTGCCATGAGCACAGTCTATAAAGGGTACATCGACGGCGGGGCAACTGTTGTTGCAATCAAGCGATTTACAGGCAGTAGACAAGACTTCAGGAATGAGGTGCTGCTGTCCTGCCAGCTGCGCCACCCCAACCTTCTCCCTGTTATTGGATTCTGTGAAGAAAAGGAGGAACTCATCCTTGTCTACGACTACATGGCCAAAGGTGCCCTCTCTTCTAAGCTGTTCAAAAGAGCGGATCTTGATCCGCTGCCCTGGAGGCAAAGGCTGAACATATGTATCGGGATAGCACGCGCATTGCATTATCTCCATGCAGGGCTAAAACTTGCTATTGTCCACCGCGACGTGAAATGCTCCATCATACTATTGGATGAAAGGTTCGAAGCAAAGGTTTCAAACTTACTAACGTCCAAGATGGGTCCTCCTAGTTTGTCAAATAAATTCATACGAGTGACCTCTGACAGGATCGTGGGCACTTTCGGGTATATTGATCCCGAGTATTTCCATTTGGAACAATTTACAGAAAAATCTGATGTCTACTCATTTGGAATGGTGCTGCTGGAAGTGTTGACTGCCAAATGGGCAAAAGAGTTTCATACATACACAGGCAGGCCGGACACCTTATGCGATATAATGGACCCTTCTTTGAGGGGTAAAATAGCTCCTGATTGTTTGCAAAAATTTATGGATATCGTTTACAGATGTGTAAGTCCTACAGGAGCAGAGCGGCCCTCAATGGGCGAAGTGCAAGTGGAACTGGAGTGCGCATTGGAGCTGCAAGACAGCGCAGATGCCAAGAAGGACGCTTGTGTTGGTCTTGCAGGTGACTATAGCTACGAAGGGATGTCAATTTGTGAAATTAAAGATATTTTCTCATTTTTGTTTAGGGCAGCTGTCAAACTTCGGATGTGTCAAGTCTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

783

Amino Acids

88.15

Weight (kDa)

7.47

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 99 - 335 8.5e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 111 - 280 3e-26 Protein kinase domain
Pkinase PF00069 469 - 723 3.3e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 472 - 726 7.3e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 284, 2234
Acc16I TGCGCA 1 cut(s) 2188
Acc36I ACCTGC 2 cut(s) 284, 2234
AccB1I GGYRCC 1 cut(s) 1628
AccBSI CCGCTC 2 cut(s) 1658, 2149
AccI GTMKAC 6 cut(s) 300, 792, 1162, 1504, 1608, 1959
AccII CGCG 5 cut(s) 137, 390, 601, 1716, 1764
AclWI GGATC 7 cut(s) 548, 561, 740, 1661, 1668, 1895, 1907
AcoI YGGCCR 2 cut(s) 766, 1620
AcsI RAATTY 3 cut(s) 929, 1862, 2102
AcuI CTGAAG 2 cut(s) 273, 1498
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 730, 1240, 1454
AfiI CCNNNNNNNGG 8 cut(s) 469, 625, 775, 986, 1333, 1571, 1884, 2159
AflIII ACRYGT 1 cut(s) 498
AjiI CACGTC 1 cut(s) 1768
AjnI CCWGG 2 cut(s) 1187, 1676
AloI GAACNNNNNNTCC 2 cut(s) 1784, 1816
Alw21I GWGCWC 2 cut(s) 40, 1439
Alw26I GTCTC 2 cut(s) 645, 1130
Alw44I GTGCAC 1 cut(s) 36
AlwI GGATC 7 cut(s) 548, 561, 740, 1661, 1668, 1895, 1907
AlwNI CAGNNNCTG 2 cut(s) 415, 1391
Ama87I CYCGRG 1 cut(s) 1916
AoxI GGCC 8 cut(s) 217, 693, 766, 977, 1335, 1620, 2033, 2150
ApaLI GTGCAC 1 cut(s) 36
ApoI RAATTY 3 cut(s) 929, 1862, 2102
AseI ATTAAT 1 cut(s) 279
AspLEI GCGC 8 cut(s) 365, 429, 482, 601, 1548, 1718, 2189, 2210
AspS9I GGNCC 7 cut(s) 257, 679, 978, 1335, 1841, 2059, 2151
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 239, 1001, 2258
AsuII TTCGAA 2 cut(s) 816, 1803
AsuNHI GCTAGC 1 cut(s) 1369
AvaI CYCGRG 1 cut(s) 1916
AvaII GGWCC 4 cut(s) 257, 679, 1841, 2059
BaeGI GKGCMC 4 cut(s) 40, 360, 1633, 1898
BaeI ACNNNNGTAYC 2 cut(s) 1684, 1717
BalI TGGCCA 2 cut(s) 768, 1622
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 1628
BanII GRGCYC 1 cut(s) 973
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
BbrPI CACGTG 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 40, 1439
BccI CCATC 5 cut(s) 718, 979, 1222, 1787, 1831
BceAI ACGGC 2 cut(s) 992, 1477
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 2 cut(s) 1189, 1678
BciVI GTATCC 1 cut(s) 302
BclI TGATCA 3 cut(s) 52, 244, 1078
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 2 cut(s) 645, 1130
BfaI CTAG 6 cut(s) 171, 884, 1065, 1289, 1370, 1848
BfmI CTRYAG 8 cut(s) 69, 291, 963, 1025, 1086, 1245, 2136, 2251
BfuAI ACCTGC 2 cut(s) 284, 2234
BfuI GTATCC 1 cut(s) 302
BglI GCCNNNNNGGC 3 cut(s) 98, 1681, 2003
BmcAI AGTACT 1 cut(s) 1240
Bme1390I CCNGG 3 cut(s) 360, 1189, 1678
Bme18I GGWCC 4 cut(s) 257, 679, 1841, 2059
BmeT110I CYCGRG 1 cut(s) 1916
BmgBI CACGTC 1 cut(s) 1768
BmgT120I GGNCC 7 cut(s) 257, 679, 978, 1335, 1841, 2059, 2151
BmiI GGNNCC 6 cut(s) 511, 555, 970, 1630, 1842, 2061
BmrFI CCNGG 3 cut(s) 360, 1189, 1678
BmrI ACTGGG 1 cut(s) 1342
BmsI GCATC 4 cut(s) 941, 1030, 1301, 2203
BmtI GCTAGC 1 cut(s) 1373
BmuI ACTGGG 1 cut(s) 1342
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 6 cut(s) 1127, 1159, 1241, 1273, 2138, 2170
BpmI CTGGAG 3 cut(s) 1040, 1699, 2201
Bpu14I TTCGAA 2 cut(s) 816, 1803
BpuEI CTTGAG 1 cut(s) 1028
BpuMI CCSGG 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 1665
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 336, 1676
BsaXI ACNNNNNCTCC 4 cut(s) 600, 630, 1054, 1084
Bsc4I CCNNNNNNNGG 8 cut(s) 469, 625, 775, 986, 1333, 1571, 1884, 2159
Bse1I ACTGG 4 cut(s) 1023, 1172, 1337, 2184
Bse3DI GCAATG 1 cut(s) 1718
Bse8I GATNNNNATC 1 cut(s) 1665
BseBI CCWGG 2 cut(s) 1189, 1678
BseDI CCNNGG 2 cut(s) 336, 1676
BseGI GGATG 4 cut(s) 1599, 1798, 2271, 2334
BseJI GATNNNNATC 1 cut(s) 1665
BseLI CCNNNNNNNGG 8 cut(s) 469, 625, 775, 986, 1333, 1571, 1884, 2159
BseMI GCAATG 1 cut(s) 1718
BseMII CTCAG 1 cut(s) 1365
BseNI ACTGG 4 cut(s) 1023, 1172, 1337, 2184
BseRI GAGGAG 2 cut(s) 1062, 1135
BseSI GKGCMC 4 cut(s) 40, 360, 1633, 1898
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 5 cut(s) 137, 390, 601, 1716, 1764
BshFI GGCC 8 cut(s) 219, 695, 768, 979, 1337, 1622, 2035, 2152
BshNI GGYRCC 1 cut(s) 1628
BsiHKAI GWGCWC 2 cut(s) 40, 1439
BsiHKCI CYCGRG 1 cut(s) 1916
BsiSI CCGG 3 cut(s) 360, 1044, 2036
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 8 cut(s) 469, 625, 775, 986, 1333, 1571, 1884, 2159
BsmAI GTCTC 2 cut(s) 645, 1130
BsmFI GGGAC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 1400
BsnI GGCC 8 cut(s) 219, 695, 768, 979, 1337, 1622, 2035, 2152
BsoBI CYCGRG 1 cut(s) 1916
Bsp119I TTCGAA 2 cut(s) 816, 1803
Bsp1286I GDGCHC 6 cut(s) 40, 360, 973, 1439, 1633, 1898
BspANI GGCC 8 cut(s) 219, 695, 768, 979, 1337, 1622, 2035, 2152
BspCNI CTCAG 1 cut(s) 1366
BspFNI CGCG 5 cut(s) 137, 390, 601, 1716, 1764
BspLI GGNNCC 6 cut(s) 511, 555, 970, 1630, 1842, 2061
BspMAI CTGCAG 4 cut(s) 295, 1029, 1090, 1249
BspMI ACCTGC 2 cut(s) 284, 2234
BspOI GCTAGC 1 cut(s) 1373
BspPI GGATC 7 cut(s) 548, 561, 740, 1661, 1668, 1895, 1907
BspT104I TTCGAA 2 cut(s) 816, 1803
BspT107I GGYRCC 1 cut(s) 1628
BsrBI CCGCTC 2 cut(s) 1658, 2149
BsrDI GCAATG 1 cut(s) 1718
BsrI ACTGG 4 cut(s) 1023, 1172, 1337, 2184
BssECI CCNNGG 2 cut(s) 336, 1676
BssNI GRCGYC 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 1189, 1678
Bst4CI ACNGT 8 cut(s) 275, 340, 417, 1094, 1103, 1216, 1441, 1474
Bst6I CTCTTC 1 cut(s) 1642
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBAI YACGTR 1 cut(s) 355
BstBI TTCGAA 2 cut(s) 816, 1803
BstDEI CTNAG 3 cut(s) 688, 1374, 1641
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 1882
BstF5I GGATG 4 cut(s) 1599, 1798, 2271, 2334
BstFNI CGCG 5 cut(s) 137, 390, 601, 1716, 1764
BstHHI GCGC 8 cut(s) 365, 429, 482, 601, 1548, 1718, 2189, 2210
BstMAI GTCTC 2 cut(s) 645, 1130
BstMWI GCNNNNNNNGC 9 cut(s) 98, 426, 872, 1094, 1253, 1545, 1628, 1681, 2003
BstNI CCWGG 2 cut(s) 1189, 1678
BstNSI RCATGY 2 cut(s) 502, 638
BstSCI CCNGG 3 cut(s) 358, 1187, 1676
BstSFI CTRYAG 8 cut(s) 69, 291, 963, 1025, 1086, 1245, 2136, 2251
BstSLI GKGCMC 4 cut(s) 40, 360, 1633, 1898
BstUI CGCG 5 cut(s) 137, 390, 601, 1716, 1764
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 553, 1660
BstYI RGATCY 2 cut(s) 553, 1660
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 8 cut(s) 219, 695, 768, 979, 1337, 1622, 2035, 2152
BtgI CCRYGG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 1768
BtsCI GGATG 4 cut(s) 1599, 1798, 2271, 2334
BtsI GCAGTG 1 cut(s) 1432
BtsIMutI CAGTG 3 cut(s) 1016, 1389, 1432
BveI ACCTGC 2 cut(s) 284, 2234
CaiI CAGNNNCTG 2 cut(s) 415, 1391
CfoI GCGC 8 cut(s) 365, 429, 482, 601, 1548, 1718, 2189, 2210
Cfr13I GGNCC 7 cut(s) 257, 679, 978, 1335, 1841, 2059, 2151
CseI GACGC 4 cut(s) 98, 396, 943, 2234
CsiI ACCWGGT 1 cut(s) 1187
Csp6I GTAC 3 cut(s) 729, 1239, 1453
CviQI GTAC 3 cut(s) 729, 1239, 1453
DdeI CTNAG 3 cut(s) 688, 1374, 1641
EaeI YGGCCR 2 cut(s) 766, 1620
Eam1104I CTCTTC 1 cut(s) 1642
EarI CTCTTC 1 cut(s) 1642
EciI GGCGGA 3 cut(s) 416, 947, 1243
Eco24I GRGCYC 1 cut(s) 973
Eco32I GATATC 2 cut(s) 1116, 2113
Eco47I GGWCC 4 cut(s) 257, 679, 1841, 2059
Eco57I CTGAAG 2 cut(s) 273, 1498
Eco72I CACGTG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 1916
EcoNI CCTNNNNNAGG 1 cut(s) 1882
EcoO109I RGGNCCY 1 cut(s) 1841
EcoRII CCWGG 2 cut(s) 1187, 1676
EcoRV GATATC 2 cut(s) 1116, 2113
EcoT38I GRGCYC 1 cut(s) 973
FaqI GGGAC 1 cut(s) 399
FauI CCCGC 3 cut(s) 375, 1364, 1457
FauNDI CATATG 2 cut(s) 861, 1697
FbaI TGATCA 3 cut(s) 52, 244, 1078
FblI GTMKAC 6 cut(s) 300, 792, 1162, 1504, 1608, 1959
FokI GGATG 4 cut(s) 1586, 1805, 2278, 2341
FriOI GRGCYC 1 cut(s) 973
FspAI RTGCGCAY 1 cut(s) 2188
FspBI CTAG 6 cut(s) 171, 884, 1065, 1289, 1370, 1848
FspI TGCGCA 1 cut(s) 2188
GlaI GCGC 8 cut(s) 364, 428, 481, 600, 1547, 1717, 2188, 2209
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 3 cut(s) 1040, 1699, 2201
HaeIII GGCC 8 cut(s) 219, 695, 768, 979, 1337, 1622, 2035, 2152
HapII CCGG 3 cut(s) 360, 1044, 2036
HgaI GACGC 4 cut(s) 98, 396, 943, 2234
HhaI GCGC 8 cut(s) 365, 429, 482, 601, 1548, 1718, 2189, 2210
Hin1I GRCGYC 1 cut(s) 90
Hin6I GCGC 8 cut(s) 363, 427, 480, 599, 1546, 1716, 2187, 2208
HinP1I GCGC 8 cut(s) 363, 427, 480, 599, 1546, 1716, 2187, 2208
HincII GTYRAC 1 cut(s) 1992
HindII GTYRAC 1 cut(s) 1992
HindIII AAGCTT 2 cut(s) 685, 698
HinfI GANTC 4 cut(s) 456, 1168, 1250, 1575
HpaII CCGG 3 cut(s) 360, 1044, 2036
HphI GGTGA 3 cut(s) 239, 1001, 2258
Hpy188I TCNGA 9 cut(s) 52, 261, 373, 455, 787, 1126, 1882, 1954, 2328
Hpy99I CGWCG 3 cut(s) 126, 1463, 1769
HpyAV CCTTC 6 cut(s) 248, 1103, 1568, 2073, 2215, 2255
HpyCH4III ACNGT 8 cut(s) 275, 340, 417, 1094, 1103, 1216, 1441, 1474
HpyCH4IV ACGT 5 cut(s) 7, 210, 354, 1767, 1829
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 426, 872, 1094, 1253, 1545, 1628, 1681, 2003
HpyF3I CTNAG 3 cut(s) 688, 1374, 1641
HpySE526I ACGT 5 cut(s) 7, 210, 354, 1767, 1829
Hsp92I GRCGYC 1 cut(s) 90
HspAI GCGC 8 cut(s) 363, 427, 480, 599, 1546, 1716, 2187, 2208
Ksp22I TGATCA 3 cut(s) 52, 244, 1078
LmnI GCTCC 7 cut(s) 916, 968, 1021, 1781, 2089, 2141, 2194
LweI GCATC 4 cut(s) 941, 1030, 1301, 2203
MabI ACCWGGT 1 cut(s) 1187
MaeI CTAG 6 cut(s) 171, 884, 1065, 1289, 1370, 1848
MaeII ACGT 5 cut(s) 7, 210, 354, 1767, 1829
MaeIII GTNAC 7 cut(s) 3, 174, 328, 662, 1207, 1873, 2246
MbiI CCGCTC 2 cut(s) 1658, 2149
MflI RGATCY 2 cut(s) 553, 1660
MhlI GDGCHC 6 cut(s) 40, 360, 973, 1439, 1633, 1898
MlsI TGGCCA 2 cut(s) 768, 1622
MluNI TGGCCA 2 cut(s) 768, 1622
MlyI GAGTC 2 cut(s) 1177, 1259
Mox20I TGGCCA 2 cut(s) 768, 1622
MscI TGGCCA 2 cut(s) 768, 1622
MseI TTAA 7 cut(s) 213, 279, 285, 440, 630, 657, 2283
MslI CAYNNNNRTG 3 cut(s) 593, 1226, 1871
Msp20I TGGCCA 2 cut(s) 768, 1622
MspA1I CMGCKG 5 cut(s) 382, 424, 1543, 1672, 2315
MspI CCGG 3 cut(s) 360, 1044, 2036
MspR9I CCNGG 3 cut(s) 360, 1189, 1678
Mva1269I GAATGC 1 cut(s) 1400
MvaI CCWGG 2 cut(s) 1189, 1678
MvnI CGCG 5 cut(s) 137, 390, 601, 1716, 1764
MwoI GCNNNNNNNGC 9 cut(s) 98, 426, 872, 1094, 1253, 1545, 1628, 1681, 2003
NciI CCSGG 1 cut(s) 360
NdeI CATATG 2 cut(s) 861, 1697
NheI GCTAGC 1 cut(s) 1369
NlaIV GGNNCC 6 cut(s) 511, 555, 970, 1630, 1842, 2061
NmuCI GTSAC 4 cut(s) 3, 662, 1873, 2246
NsbI TGCGCA 1 cut(s) 2188
NspI RCATGY 2 cut(s) 502, 638
NspV TTCGAA 2 cut(s) 816, 1803
PaeI GCATGC 1 cut(s) 638
PaqCI CACCTGC 2 cut(s) 284, 2234
PciI ACATGT 1 cut(s) 498
PctI GAATGC 1 cut(s) 1400
PfeI GAWTC 2 cut(s) 456, 1575
PflFI GACNNNGTC 2 cut(s) 941, 1167
PleI GAGTC 2 cut(s) 1176, 1258
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 2 cut(s) 1176, 1258
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 1841
PscI ACATGT 1 cut(s) 498
PshBI ATTAAT 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 1841
Psp6I CCWGG 2 cut(s) 1187, 1676
PspCI CACGTG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 2 cut(s) 1187, 1676
PspN4I GGNNCC 6 cut(s) 511, 555, 970, 1630, 1842, 2061
PspPI GGNCC 7 cut(s) 257, 679, 978, 1335, 1841, 2059, 2151
PspPPI RGGWCCY 1 cut(s) 1841
PstI CTGCAG 4 cut(s) 295, 1029, 1090, 1249
PstNI CAGNNNCTG 2 cut(s) 415, 1391
PsuI RGATCY 2 cut(s) 553, 1660
PsyI GACNNNGTC 2 cut(s) 941, 1167
PvuII CAGCTG 3 cut(s) 424, 1543, 2315
RsaI GTAC 3 cut(s) 730, 1240, 1454
RsaNI GTAC 3 cut(s) 729, 1239, 1453
RseI CAYNNNNRTG 3 cut(s) 593, 1226, 1871
SaqAI TTAA 7 cut(s) 213, 279, 285, 440, 630, 657, 2283
Sau96I GGNCC 7 cut(s) 257, 679, 978, 1335, 1841, 2059, 2151
ScaI AGTACT 1 cut(s) 1240
SchI GAGTC 2 cut(s) 1177, 1259
ScrFI CCNGG 3 cut(s) 360, 1189, 1678
SduI GDGCHC 6 cut(s) 40, 360, 973, 1439, 1633, 1898
SexAI ACCWGGT 1 cut(s) 1187
SfaNI GCATC 4 cut(s) 941, 1030, 1301, 2203
SfcI CTRYAG 8 cut(s) 69, 291, 963, 1025, 1086, 1245, 2136, 2251
SfuI TTCGAA 2 cut(s) 816, 1803
SinI GGWCC 4 cut(s) 257, 679, 1841, 2059
SmiMI CAYNNNNRTG 3 cut(s) 593, 1226, 1871
SmlI CTYRAG 1 cut(s) 1007
SmoI CTYRAG 1 cut(s) 1007
SphI GCATGC 1 cut(s) 638
SspI AATATT 1 cut(s) 836
SspMI CTAG 6 cut(s) 171, 884, 1065, 1289, 1370, 1848
StyD4I CCNGG 3 cut(s) 358, 1187, 1676
TaaI ACNGT 8 cut(s) 275, 340, 417, 1094, 1103, 1216, 1441, 1474
TaiI ACGT 5 cut(s) 10, 213, 357, 1770, 1832
TaqI TCGA 3 cut(s) 816, 1458, 1803
TatI WGTACW 1 cut(s) 1238
TauI GCSGC 1 cut(s) 2152
TfiI GAWTC 2 cut(s) 456, 1575
Tru1I TTAA 7 cut(s) 213, 279, 285, 440, 630, 657, 2283
Tru9I TTAA 7 cut(s) 213, 279, 285, 440, 630, 657, 2283
TscAI CASTG 3 cut(s) 1023, 1396, 1432
TseFI GTSAC 4 cut(s) 3, 662, 1873, 2246
Tsp45I GTSAC 4 cut(s) 3, 662, 1873, 2246
TspDTI ATGAA 4 cut(s) 1122, 1809, 1855, 2006
TspGWI ACGGA 2 cut(s) 546, 791
TspRI CASTG 3 cut(s) 1023, 1396, 1432
Tth111I GACNNNGTC 2 cut(s) 941, 1167
VneI GTGCAC 1 cut(s) 36
VpaK11BI GGWCC 4 cut(s) 257, 679, 1841, 2059
VspI ATTAAT 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 1882
XapI RAATTY 3 cut(s) 929, 1862, 2102
XbaI TCTAGA 1 cut(s) 883
XceI RCATGY 2 cut(s) 502, 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 1786
XmiI GTMKAC 6 cut(s) 300, 792, 1162, 1504, 1608, 1959
XspI CTAG 6 cut(s) 171, 884, 1065, 1289, 1370, 1848
ZrmI AGTACT 1 cut(s) 1240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.