MD04G1238400.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009726
Physical Location & Seq
Reverse (-)
31665753 .. 31666910
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1238400.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGATGGGTTTCAGTACTACTGCAGAGTCCGCCTCATGTTTCTTCTTCTCTGCATGGAAGAAACTAGCATCATGTGATAAAAGTAGGAAACGCAGACAAAACCAAGACTGGGCCTTTATGGAGGAATTATCCCGCCGATTTTCGCTAGCTGAGATCATAGCAGCCACTGAATGCTTCAACGACAAGTTCCATATTGGTAGCAGTGCCATGAGCACAGTCTATAAAGGGTACATCGACGGCGGGGCAACTGTTGTTGCAATCAAGCGATTTACAGGCAGTAGACAAGACTTCAGGAATGAGGTGCTGCTGTCCTGCCAGCTGCGCCACCCCAACCTTCTCCCTGTTATTGGATTCTGTGAAGAAAAGGAGGAACTCATCCTTGTCTACGACTACATGGCCAAAGGTGCCCTCTCTTCTAAGCTGTTCAAAAGAGCGGATCTTGATCCGCTGCCCTGGAGGCAAAGGCTGAACATATGTATCGGGATAGCACGCGCATTGCATTATCTCCATGCAGGGCTAAAACTTGCTATTGTCCACCGCGACGTGAAATGCTCCATCATACTATTGGATGAAAGGTTCGAAGCAAAGGTTTCAAACTTACTAACGTCCAAGATGGGTCCTCCTAGTTTGTCAAATAAATTCATACGAGTGACCTCTGACAGGATCGTGGGCACTTTCGGGTATATTGATCCCGAGTATTTCCATTTGGAACAATTTACAGAAAAATCTGATGTCTACTCATTTGGAATGGTGCTGCTGGAAGTGTTGACTGCCAAATGGGCAAAAGAGTTTCATACATACACAGGCAGGCCGGACACCTTATGCGATATAATGGACCCTTCTTTGAGGGGTAAAATAGCTCCTGATTGTTTGCAAAAATTTATGGATATCGTTTACAGATGTGTAAGTCCTACAGGAGCAGAGCGGCCCTCAATGGGCGAAGTGCAAGTGGAACTGGAGTGCGCATTGGAGCTGCAAGACAGCGCAGATGCCAAGAAGGACGCTTGTGTTGGTCTTGCAGGTGACTATAGCTACGAAGGGATGTCAATTTGTGAAATTAAAGATATTTTCTCATTTTTGTTTAGGCAGCTGTCAACTTCGGATGTGTCAAGTCTTGATTTGAATAGCAACGATTTTTCTGCTTCTGATGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

386

Amino Acids

43.35

Weight (kDa)

5.76

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 61 - 315 5.8e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 64 - 318 1.3e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
Acc16I TGCGCA 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 1010
AccB1I GGYRCC 1 cut(s) 404
AccBSI CCGCTC 2 cut(s) 434, 925
AccI GTMKAC 3 cut(s) 280, 384, 735
AccII CGCG 2 cut(s) 492, 540
AciI CCGC 7 cut(s) 30, 133, 240, 434, 446, 538, 925
AclWI GGATC 4 cut(s) 437, 444, 671, 683
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 638, 878
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 2 cut(s) 16, 230
AfiI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
AgsI TTSAA 4 cut(s) 178, 427, 594, 1123
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 7 cut(s) 149, 319, 421, 860, 973, 1032, 1090
AluI AGCT 7 cut(s) 149, 319, 421, 860, 973, 1032, 1090
Alw21I GWGCWC 1 cut(s) 215
AlwI GGATC 4 cut(s) 437, 444, 671, 683
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 692
AoxI GGCC 4 cut(s) 111, 396, 809, 926
ApeKI GCWGC 7 cut(s) 161, 304, 319, 448, 754, 973, 1087
ApoI RAATTY 2 cut(s) 638, 878
AspLEI GCGC 4 cut(s) 324, 494, 965, 986
AspS9I GGNCC 4 cut(s) 111, 617, 835, 927
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 1 cut(s) 579
AsuNHI GCTAGC 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 692
AvaII GGWCC 2 cut(s) 617, 835
BaeGI GKGCMC 2 cut(s) 409, 674
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BalI TGGCCA 1 cut(s) 398
BanI GGYRCC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 7 cut(s) 173, 291, 306, 435, 741, 960, 1099
BccI CCATC 3 cut(s) 563, 607, 1145
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 1 cut(s) 454
BfaI CTAG 3 cut(s) 65, 146, 624
BfmI CTRYAG 3 cut(s) 21, 912, 1027
BfuAI ACCTGC 1 cut(s) 1010
BglI GCCNNNNNGGC 2 cut(s) 457, 779
BisI GCNGC 8 cut(s) 162, 305, 320, 449, 755, 926, 974, 1088
BlsI GCNGC 8 cut(s) 163, 306, 321, 450, 756, 927, 975, 1089
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 454
Bme18I GGWCC 2 cut(s) 617, 835
BmeT110I CYCGRG 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 4 cut(s) 111, 617, 835, 927
BmiI GGNNCC 3 cut(s) 406, 618, 837
BmrFI CCNGG 1 cut(s) 454
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 77, 979
BmtI GCTAGC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 118
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 914, 946
BpmI CTGGAG 2 cut(s) 475, 977
Bpu14I TTCGAA 1 cut(s) 579
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 452
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
Bse1I ACTGG 2 cut(s) 113, 960
Bse3DI GCAATG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 4 cut(s) 375, 574, 1047, 1108
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 2 cut(s) 113, 960
BseSI GKGCMC 2 cut(s) 409, 674
BseXI GCAGC 7 cut(s) 173, 291, 306, 435, 741, 960, 1099
Bsh1236I CGCG 2 cut(s) 492, 540
BshFI GGCC 4 cut(s) 113, 398, 811, 928
BshNI GGYRCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 692
BsiSI CCGG 1 cut(s) 812
BslI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 4 cut(s) 113, 398, 811, 928
BsoBI CYCGRG 1 cut(s) 692
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 3 cut(s) 215, 409, 674
Bsp143I GATC 5 cut(s) 153, 436, 442, 663, 688
BspACI CCGC 7 cut(s) 30, 133, 240, 434, 446, 538, 925
BspANI GGCC 4 cut(s) 113, 398, 811, 928
BspCNI CTCAG 1 cut(s) 142
BspFNI CGCG 2 cut(s) 492, 540
BspLI GGNNCC 3 cut(s) 406, 618, 837
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 1010
BspOI GCTAGC 1 cut(s) 149
BspPI GGATC 4 cut(s) 437, 444, 671, 683
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 404
BsrBI CCGCTC 2 cut(s) 434, 925
BsrDI GCAATG 1 cut(s) 494
BsrI ACTGG 2 cut(s) 113, 960
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 5 cut(s) 153, 436, 442, 663, 688
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 217, 250
Bst6I CTCTTC 1 cut(s) 418
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 4 cut(s) 147, 317, 490, 809
BstDEI CTNAG 2 cut(s) 150, 417
BstENI CCTNNNNNAGG 1 cut(s) 658
BstF5I GGATG 4 cut(s) 375, 574, 1047, 1108
BstFNI CGCG 2 cut(s) 492, 540
BstHHI GCGC 4 cut(s) 324, 494, 965, 986
BstKTI GATC 5 cut(s) 156, 439, 445, 666, 691
BstMBI GATC 5 cut(s) 153, 436, 442, 663, 688
BstMWI GCNNNNNNNGC 5 cut(s) 29, 321, 404, 457, 779
BstNI CCWGG 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 452
BstSFI CTRYAG 3 cut(s) 21, 912, 1027
BstSLI GKGCMC 2 cut(s) 409, 674
BstUI CGCG 2 cut(s) 492, 540
BstV1I GCAGC 7 cut(s) 173, 291, 306, 435, 741, 960, 1099
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 4 cut(s) 113, 398, 811, 928
BtrI CACGTC 1 cut(s) 544
BtsCI GGATG 4 cut(s) 375, 574, 1047, 1108
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 165, 208
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 4 cut(s) 147, 317, 490, 809
CaiI CAGNNNCTG 1 cut(s) 167
CfoI GCGC 4 cut(s) 324, 494, 965, 986
Cfr13I GGNCC 4 cut(s) 111, 617, 835, 927
CseI GACGC 1 cut(s) 1010
Csp6I GTAC 2 cut(s) 15, 229
CviAII CATG 6 cut(s) 36, 54, 72, 208, 394, 509
CviQI GTAC 2 cut(s) 15, 229
DdeI CTNAG 2 cut(s) 150, 417
DpnI GATC 5 cut(s) 155, 438, 444, 665, 690
DpnII GATC 5 cut(s) 153, 436, 442, 663, 688
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
EciI GGCGGA 1 cut(s) 19
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 2 cut(s) 617, 835
Eco57I CTGAAG 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 658
EcoO109I RGGNCCY 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 452
EcoRV GATATC 1 cut(s) 889
FaeI CATG 6 cut(s) 39, 57, 75, 211, 397, 512
FatI CATG 6 cut(s) 35, 53, 71, 207, 393, 508
FauI CCCGC 2 cut(s) 140, 233
FauNDI CATATG 1 cut(s) 473
FblI GTMKAC 3 cut(s) 280, 384, 735
Fnu4HI GCNGC 8 cut(s) 162, 305, 320, 449, 755, 926, 974, 1088
FokI GGATG 4 cut(s) 362, 581, 1054, 1115
Fsp4HI GCNGC 8 cut(s) 162, 305, 320, 449, 755, 926, 974, 1088
FspAI RTGCGCAY 1 cut(s) 964
FspBI CTAG 3 cut(s) 65, 146, 624
FspI TGCGCA 1 cut(s) 964
GlaI GCGC 4 cut(s) 323, 493, 964, 985
GluI GCNGC 8 cut(s) 162, 305, 320, 449, 755, 926, 974, 1088
GsuI CTGGAG 2 cut(s) 475, 977
HaeIII GGCC 4 cut(s) 113, 398, 811, 928
HapII CCGG 1 cut(s) 812
HgaI GACGC 1 cut(s) 1010
HhaI GCGC 4 cut(s) 324, 494, 965, 986
Hin1II CATG 6 cut(s) 39, 57, 75, 211, 397, 512
Hin6I GCGC 4 cut(s) 322, 492, 963, 984
HinP1I GCGC 4 cut(s) 322, 492, 963, 984
HincII GTYRAC 2 cut(s) 768, 1095
HindII GTYRAC 2 cut(s) 768, 1095
HinfI GANTC 2 cut(s) 26, 351
HpaII CCGG 1 cut(s) 812
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 7 cut(s) 281, 385, 535, 736, 768, 895, 1095
Hpy188I TCNGA 4 cut(s) 658, 730, 1102, 1147
Hpy188III TCNNGA 6 cut(s) 292, 440, 481, 692, 863, 1115
Hpy8I GTNNAC 7 cut(s) 281, 385, 535, 736, 768, 895, 1095
Hpy99I CGWCG 2 cut(s) 239, 545
HpyAV CCTTC 4 cut(s) 344, 849, 991, 1031
HpyCH4III ACNGT 2 cut(s) 217, 250
HpyCH4IV ACGT 2 cut(s) 543, 605
HpyCH4V TGCA 9 cut(s) 23, 53, 257, 499, 512, 874, 946, 976, 1019
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 321, 404, 457, 779
HpyF3I CTNAG 2 cut(s) 150, 417
HpySE526I ACGT 2 cut(s) 543, 605
Hsp92II CATG 6 cut(s) 39, 57, 75, 211, 397, 512
HspAI GCGC 4 cut(s) 322, 492, 963, 984
Kzo9I GATC 5 cut(s) 153, 436, 442, 663, 688
LmnI GCTCC 4 cut(s) 557, 865, 917, 970
Lsp1109I GCAGC 7 cut(s) 173, 291, 306, 435, 741, 960, 1099
LweI GCATC 2 cut(s) 77, 979
MaeI CTAG 3 cut(s) 65, 146, 624
MaeII ACGT 2 cut(s) 543, 605
MaeIII GTNAC 2 cut(s) 649, 1022
MalI GATC 5 cut(s) 155, 438, 444, 665, 690
MbiI CCGCTC 2 cut(s) 434, 925
MboI GATC 5 cut(s) 153, 436, 442, 663, 688
MboII GAAGA 5 cut(s) 34, 37, 70, 371, 405
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 215, 409, 674
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 1 cut(s) 35
Mox20I TGGCCA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 398
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 3 cut(s) 319, 448, 1090
MspI CCGG 1 cut(s) 812
MspR9I CCNGG 1 cut(s) 454
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 2 cut(s) 492, 540
MwoI GCNNNNNNNGC 5 cut(s) 29, 321, 404, 457, 779
NdeI CATATG 1 cut(s) 473
NdeII GATC 5 cut(s) 153, 436, 442, 663, 688
NheI GCTAGC 1 cut(s) 145
NlaIII CATG 6 cut(s) 39, 57, 75, 211, 397, 512
NlaIV GGNNCC 3 cut(s) 406, 618, 837
NmuCI GTSAC 2 cut(s) 649, 1022
NsbI TGCGCA 1 cut(s) 964
NspV TTCGAA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 1010
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 8 cut(s) 163, 306, 321, 450, 756, 927, 975, 1089
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 617
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 406, 618, 837
PspPI GGNCC 4 cut(s) 111, 617, 835, 927
PspPPI RGGWCCY 1 cut(s) 617
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 319, 1090
RsaI GTAC 2 cut(s) 16, 230
RsaNI GTAC 2 cut(s) 15, 229
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 1 cut(s) 1059
SatI GCNGC 8 cut(s) 162, 305, 320, 449, 755, 926, 974, 1088
Sau3AI GATC 5 cut(s) 153, 436, 442, 663, 688
Sau96I GGNCC 4 cut(s) 111, 617, 835, 927
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 454
SduI GDGCHC 3 cut(s) 215, 409, 674
SfaNI GCATC 2 cut(s) 77, 979
SfcI CTRYAG 3 cut(s) 21, 912, 1027
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 2 cut(s) 617, 835
SmiMI CAYNNNNRTG 1 cut(s) 647
Sse9I AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
SsiI CCGC 7 cut(s) 30, 133, 240, 434, 446, 538, 925
SspMI CTAG 3 cut(s) 65, 146, 624
StyD4I CCNGG 1 cut(s) 452
TaaI ACNGT 2 cut(s) 217, 250
TaiI ACGT 2 cut(s) 546, 608
TaqI TCGA 2 cut(s) 234, 579
TasI AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 928
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 2 cut(s) 172, 208
TseFI GTSAC 2 cut(s) 649, 1022
TseI GCWGC 7 cut(s) 161, 304, 319, 448, 754, 973, 1087
Tsp45I GTSAC 2 cut(s) 649, 1022
TspDTI ATGAA 3 cut(s) 585, 631, 782
TspRI CASTG 2 cut(s) 172, 208
VpaK11BI GGWCC 2 cut(s) 617, 835
XagI CCTNNNNNAGG 1 cut(s) 658
XapI RAATTY 2 cut(s) 638, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 3 cut(s) 280, 384, 735
XspI CTAG 3 cut(s) 65, 146, 624
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.