MD12G1256200.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009726
Physical Location & Seq
Forward (+)
32387454 .. 32390885
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1256200.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGTCACGTTTCTTCAATAATTTGTGGAAGAAAGGTGCACCATATTCATCCGATCATAGAAGAAAACCTTCGGATCCAGATCGAGATGAAGCTAAATGGGCGTTTCCAGAAGAATTGTGTCGTCGGTTTTCACTCGCGGAGATCATAGCAGCAACTCAGAACTTCAATGCGAGGTTACGGATTGGCAGTACCAGAAATTTGTCATTTAAAATTAATGGCCTAATAGATAAGTTTTTGGTGTTCGATCGCCTTGAGGGTCCGAAAACAAGAAAAGACGGTGTTAATGTCGATGCTGCAGGTGTCGTCTACAAAGGATACATCAACCTCGACGGGGTAACTTCCACGGTTGCTATAAAGCGTATTACAGGTGGACATTCATATATTCAGCAGGAACGCGAGTTCAAGGCGGAGGTGCAGCTACTGTGTCAGTTGCGCCACCCCAATTTAATCTCGCTAATCGGATTCTGCCAAGAAAAGGGAGAGTGCATCATTGTTTGCAACTACATGTCCAATGGTCCCCTCTCTGACTTCCTTTTCAATCCTTATAACAATAATAGGTATCCGTTGTCTTGGAAGCAACGGCTGAATATCTGCATCGAGGTGGCGCGCGGAATACACTACCTTCACGCGGGGGTTAAGCATGCGGTCATCCATCGCAACCTGAAATGCGACACCATTCTATTGGATCAAAGCTTGGAGGCCAGGCTTTCTAACTTCCGGCTGTCCAAGATGGGTCCTCCTACTTTGTCAAACGCATTAATCAGATTGAACTCCAATTCCGGGGTGTCAGGTACATTCGGGTATCTTGATCCAGAGTATGCTTTGTCCGGCCAGCTTACGGAAAAATCTGATGTCTTCTCATTCGGAGTGGTGTTATTCGAAATATTGTCTGCCAAACATTCGACAGAGATACCAGGAATACATATGACTCTCCCAGCTGATATTCTGGATCCATCTCTCATGGGAACAACAGCTCCGGATTGTTTGATAAAATATTTGGACATCATCCAAAGATGTGTCCGCCCTACGGGAGCCCAACGGCCAACGATGGGTGAAGCGGAGGTGGAACTTGAGCATGCGGTGGAGCTGCAGGAGAGAGCAGATGCCGAAAAGGAGTTGAGGACAACTATCACTCCTCTTGGTCGTCCTGCTGAATGTGCCTACGATTATCAAGGGATATCAGTTTCCGAAATTAATGATGCTTTCTCGTTTCTGAGAGTTAGCCCGACCGGGTCTGCTGATCTTGGTTCAAATAGTATTAACGTTTCAGTACTTTCTGGTGACAGTGGCTCTGGATTCGAATATTCCGGGCGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

440

Amino Acids

48.78

Weight (kDa)

6.8

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 99 - 353 5.2e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 100 - 352 1.4e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
AarI CACCTGC 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 306
AccII CGCG 5 cut(s) 137, 396, 607, 609, 629
AccIII TCCGGA 1 cut(s) 976
AciI CCGC 8 cut(s) 137, 407, 609, 629, 644, 1021, 1058, 1079
AclI AACGTT 1 cut(s) 1263
AclWI GGATC 6 cut(s) 68, 81, 693, 803, 944, 957
AcoI YGGCCR 2 cut(s) 829, 1040
AcsI RAATTY 1 cut(s) 196
AfaI GTAC 3 cut(s) 190, 793, 1272
AfiI CCNNNNNNNGG 7 cut(s) 331, 475, 628, 780, 838, 1027, 1049
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 6 cut(s) 16, 166, 403, 538, 769, 1251
AjnI CCWGG 2 cut(s) 701, 913
AloI GAACNNNNNNTCC 6 cut(s) 383, 415, 761, 793, 958, 990
AluBI AGCT 8 cut(s) 92, 418, 693, 835, 938, 974, 1087, 1316
AluI AGCT 8 cut(s) 92, 418, 693, 835, 938, 974, 1087, 1316
Alw21I GWGCWC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 36
AlwI GGATC 6 cut(s) 68, 81, 693, 803, 944, 957
AlwNI CAGNNNCTG 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 976
AoxI GGCC 4 cut(s) 217, 699, 829, 1040
ApaLI GTGCAC 1 cut(s) 36
ApeKI GCWGC 4 cut(s) 149, 293, 415, 1087
ApoI RAATTY 1 cut(s) 196
AseI ATTAAT 3 cut(s) 213, 758, 1194
Asp700I GAANNNNTTC 1 cut(s) 67
AspLEI GCGC 3 cut(s) 435, 607, 609
AspS9I GGNCC 3 cut(s) 257, 515, 734
AsuC2I CCSGG 3 cut(s) 781, 1231, 1309
AsuHPI GGTGA 2 cut(s) 1064, 1292
AsuII TTCGAA 2 cut(s) 879, 1299
AvaII GGWCC 3 cut(s) 257, 515, 734
BaeGI GKGCMC 1 cut(s) 40
BamHI GGATCC 2 cut(s) 73, 949
BanII GRGCYC 1 cut(s) 1036
BbsI GAAGAC 1 cut(s) 847
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 161, 280, 427, 1074
BccI CCATC 4 cut(s) 660, 724, 961, 1042
BceAI ACGGC 2 cut(s) 596, 1055
BciT130I CCWGG 2 cut(s) 703, 915
BciVI GTATCC 2 cut(s) 308, 570
BcnI CCSGG 3 cut(s) 781, 1231, 1309
BfmI CTRYAG 2 cut(s) 294, 1088
BfuAI ACCTGC 1 cut(s) 287
BfuI GTATCC 2 cut(s) 308, 570
BisI GCNGC 4 cut(s) 150, 294, 416, 1088
BlsI GCNGC 4 cut(s) 151, 295, 417, 1089
BmcAI AGTACT 1 cut(s) 1272
Bme1390I CCNGG 5 cut(s) 703, 781, 915, 1231, 1309
Bme18I GGWCC 3 cut(s) 257, 515, 734
BmgT120I GGNCC 3 cut(s) 257, 515, 734
BmiI GGNNCC 6 cut(s) 75, 258, 517, 735, 951, 1033
BmrFI CCNGG 5 cut(s) 703, 781, 915, 1231, 1309
BmsI GCATC 5 cut(s) 280, 495, 603, 1093, 1189
BpiI GAAGAC 1 cut(s) 847
Bpu14I TTCGAA 2 cut(s) 879, 1299
BpuEI CTTGAG 2 cut(s) 272, 1091
BpuMI CCSGG 3 cut(s) 781, 1231, 1309
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 342, 780
BsaWI WCCGGW 1 cut(s) 976
BsaXI ACNNNNNCTCC 4 cut(s) 958, 988, 1117, 1147
Bsc4I CCNNNNNNNGG 7 cut(s) 331, 475, 628, 780, 838, 1027, 1049
Bse8I GATNNNNATC 1 cut(s) 78
BseAI TCCGGA 1 cut(s) 976
BseBI CCWGG 2 cut(s) 703, 915
BseDI CCNNGG 2 cut(s) 342, 780
BseGI GGATG 3 cut(s) 47, 648, 1005
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 7 cut(s) 331, 475, 628, 780, 838, 1027, 1049
BseMII CTCAG 2 cut(s) 170, 1205
BsePI GCGCGC 1 cut(s) 605
BseRI GAGGAG 1 cut(s) 1125
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 4 cut(s) 161, 280, 427, 1074
BseYI CCCAGC 1 cut(s) 934
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 5 cut(s) 137, 396, 607, 609, 629
Bsh1285I CGRYCG 2 cut(s) 247, 1230
BshFI GGCC 4 cut(s) 219, 701, 831, 1042
BsiEI CGRYCG 2 cut(s) 247, 1230
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 6 cut(s) 718, 780, 828, 977, 1230, 1308
BslFI GGGAC 1 cut(s) 501
BslI CCNNNNNNNGG 7 cut(s) 331, 475, 628, 780, 838, 1027, 1049
BsmFI GGGAC 1 cut(s) 501
BsnI GGCC 4 cut(s) 219, 701, 831, 1042
Bsp119I TTCGAA 2 cut(s) 879, 1299
Bsp1286I GDGCHC 2 cut(s) 40, 1036
Bsp13I TCCGGA 1 cut(s) 976
Bsp143I GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
BspACI CCGC 8 cut(s) 137, 407, 609, 629, 644, 1021, 1058, 1079
BspANI GGCC 4 cut(s) 219, 701, 831, 1042
BspCNI CTCAG 2 cut(s) 169, 1206
BspEI TCCGGA 1 cut(s) 976
BspFNI CGCG 5 cut(s) 137, 396, 607, 609, 629
BspLI GGNNCC 6 cut(s) 75, 258, 517, 735, 951, 1033
BspMAI CTGCAG 2 cut(s) 298, 1092
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 6 cut(s) 68, 81, 693, 803, 944, 957
BspT104I TTCGAA 2 cut(s) 879, 1299
BssECI CCNNGG 2 cut(s) 342, 780
BssHII GCGCGC 1 cut(s) 605
BssMI GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
Bst2UI CCWGG 2 cut(s) 703, 915
Bst4CI ACNGT 4 cut(s) 278, 346, 423, 1286
BstBI TTCGAA 2 cut(s) 879, 1299
BstC8I GCNNGC 5 cut(s) 607, 642, 833, 1077, 1314
BstDEI CTNAG 3 cut(s) 156, 1214, 1317
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 3 cut(s) 47, 648, 1005
BstFNI CGCG 5 cut(s) 137, 396, 607, 609, 629
BstHHI GCGC 3 cut(s) 435, 607, 609
BstKTI GATC 9 cut(s) 55, 76, 82, 144, 247, 688, 811, 952, 1243
BstMBI GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
BstMCI CGRYCG 2 cut(s) 247, 1230
BstMWI GCNNNNNNNGC 2 cut(s) 98, 1157
BstNI CCWGG 2 cut(s) 703, 915
BstNSI RCATGY 3 cut(s) 508, 644, 1079
BstSCI CCNGG 5 cut(s) 701, 779, 913, 1229, 1307
BstSFI CTRYAG 2 cut(s) 294, 1088
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 5 cut(s) 137, 396, 607, 609, 629
BstV1I GCAGC 4 cut(s) 161, 280, 427, 1074
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 2 cut(s) 73, 949
BstXI CCANNNNNNTGG 1 cut(s) 682
BstYI RGATCY 2 cut(s) 73, 949
BsuI GTATCC 2 cut(s) 308, 570
BsuRI GGCC 4 cut(s) 219, 701, 831, 1042
BtgI CCRYGG 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 638
BtsCI GGATG 3 cut(s) 47, 648, 1005
BtsIMutI CAGTG 1 cut(s) 1291
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 5 cut(s) 607, 642, 833, 1077, 1314
CaiI CAGNNNCTG 1 cut(s) 421
CfoI GCGC 3 cut(s) 435, 607, 609
Cfr13I GGNCC 3 cut(s) 257, 515, 734
Csp6I GTAC 3 cut(s) 189, 792, 1271
CviAII CATG 4 cut(s) 505, 641, 961, 1076
CviQI GTAC 3 cut(s) 189, 792, 1271
DdeI CTNAG 3 cut(s) 156, 1214, 1317
DpnI GATC 9 cut(s) 54, 75, 81, 143, 246, 687, 810, 951, 1242
DpnII GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 829, 1040
EciI GGCGGA 2 cut(s) 422, 1010
Eco24I GRGCYC 1 cut(s) 1036
Eco32I GATATC 1 cut(s) 1179
Eco47I GGWCC 3 cut(s) 257, 515, 734
EcoO109I RGGNCCY 1 cut(s) 734
EcoRII CCWGG 2 cut(s) 701, 913
EcoRV GATATC 1 cut(s) 1179
EcoT38I GRGCYC 1 cut(s) 1036
FaeI CATG 4 cut(s) 508, 644, 964, 1079
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 501
FatI CATG 4 cut(s) 504, 640, 960, 1075
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 150, 294, 416, 1088
FokI GGATG 3 cut(s) 34, 635, 992
FriOI GRGCYC 1 cut(s) 1036
Fsp4HI GCNGC 4 cut(s) 150, 294, 416, 1088
GlaI GCGC 3 cut(s) 434, 606, 608
GluI GCNGC 4 cut(s) 150, 294, 416, 1088
GsaI CCCAGC 1 cut(s) 938
HaeIII GGCC 4 cut(s) 219, 701, 831, 1042
HapII CCGG 6 cut(s) 718, 780, 828, 977, 1230, 1308
HhaI GCGC 3 cut(s) 435, 607, 609
Hin1II CATG 4 cut(s) 508, 644, 964, 1079
Hin6I GCGC 3 cut(s) 433, 605, 607
HinP1I GCGC 3 cut(s) 433, 605, 607
HindIII AAGCTT 1 cut(s) 691
HinfI GANTC 3 cut(s) 462, 928, 1296
HpaII CCGG 6 cut(s) 718, 780, 828, 977, 1230, 1308
HphI GGTGA 2 cut(s) 1064, 1292
Hpy166II GTNNAC 3 cut(s) 38, 307, 371
Hpy188III TCNNGA 8 cut(s) 77, 83, 107, 806, 812, 947, 977, 1293
Hpy8I GTNNAC 3 cut(s) 38, 307, 371
Hpy99I CGWCG 2 cut(s) 126, 332
HpyAV CCTTC 2 cut(s) 78, 632
HpyCH4III ACNGT 4 cut(s) 278, 346, 423, 1286
HpyCH4IV ACGT 2 cut(s) 7, 1263
HpyCH4V TGCA 7 cut(s) 38, 296, 415, 486, 498, 594, 1090
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 1157
HpyF3I CTNAG 3 cut(s) 156, 1214, 1317
HpySE526I ACGT 2 cut(s) 7, 1263
Hsp92II CATG 4 cut(s) 508, 644, 964, 1079
HspAI GCGC 3 cut(s) 433, 605, 607
Kpn2I TCCGGA 1 cut(s) 976
Kzo9I GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
LmnI GCTCC 3 cut(s) 979, 1031, 1084
Lsp1109I GCAGC 4 cut(s) 161, 280, 427, 1074
LweI GCATC 5 cut(s) 280, 495, 603, 1093, 1189
MaeII ACGT 2 cut(s) 7, 1263
MaeIII GTNAC 4 cut(s) 3, 174, 334, 1280
MalI GATC 9 cut(s) 54, 75, 81, 143, 246, 687, 810, 951, 1242
MboI GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
MboII GAAGA 5 cut(s) 4, 40, 72, 122, 847
MflI RGATCY 2 cut(s) 73, 949
MhlI GDGCHC 2 cut(s) 40, 1036
MluCI AATT 7 cut(s) 19, 113, 196, 210, 442, 775, 1191
MlyI GAGTC 1 cut(s) 922
MroI TCCGGA 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 67
MseI TTAA 8 cut(s) 207, 213, 282, 446, 636, 758, 1194, 1260
MslI CAYNNNNRTG 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 6 cut(s) 718, 780, 828, 977, 1230, 1308
MspR9I CCNGG 5 cut(s) 703, 781, 915, 1231, 1309
MvaI CCWGG 2 cut(s) 703, 915
MvnI CGCG 5 cut(s) 137, 396, 607, 609, 629
MwoI GCNNNNNNNGC 2 cut(s) 98, 1157
NciI CCSGG 3 cut(s) 781, 1231, 1309
NdeI CATATG 1 cut(s) 924
NdeII GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
NlaIII CATG 4 cut(s) 508, 644, 964, 1079
NlaIV GGNNCC 6 cut(s) 75, 258, 517, 735, 951, 1033
NmuCI GTSAC 2 cut(s) 3, 1280
NspI RCATGY 3 cut(s) 508, 644, 1079
NspV TTCGAA 2 cut(s) 879, 1299
PaeI GCATGC 2 cut(s) 644, 1079
PaqCI CACCTGC 1 cut(s) 287
PauI GCGCGC 1 cut(s) 605
PciI ACATGT 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 462, 1296
PflFI GACNNNGTC 1 cut(s) 1231
PkrI GCNGC 4 cut(s) 151, 295, 417, 1089
Ple19I CGATCG 1 cut(s) 247
PleI GAGTC 1 cut(s) 922
PpsI GAGTC 1 cut(s) 922
PpuMI RGGWCCY 1 cut(s) 734
PscI ACATGT 1 cut(s) 504
PshBI ATTAAT 3 cut(s) 213, 758, 1194
PsiI TTATAA 1 cut(s) 546
Psp1406I AACGTT 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 734
Psp6I CCWGG 2 cut(s) 701, 913
PspFI CCCAGC 1 cut(s) 934
PspGI CCWGG 2 cut(s) 701, 913
PspN4I GGNNCC 6 cut(s) 75, 258, 517, 735, 951, 1033
PspPI GGNCC 3 cut(s) 257, 515, 734
PspPPI RGGWCCY 1 cut(s) 734
PstI CTGCAG 2 cut(s) 298, 1092
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 73, 949
PsyI GACNNNGTC 1 cut(s) 1231
PteI GCGCGC 1 cut(s) 605
PvuI CGATCG 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 3 cut(s) 190, 793, 1272
RsaNI GTAC 3 cut(s) 189, 792, 1271
RseI CAYNNNNRTG 1 cut(s) 599
SaqAI TTAA 8 cut(s) 207, 213, 282, 446, 636, 758, 1194, 1260
SatI GCNGC 4 cut(s) 150, 294, 416, 1088
Sau3AI GATC 9 cut(s) 52, 73, 79, 141, 244, 685, 808, 949, 1240
Sau96I GGNCC 3 cut(s) 257, 515, 734
ScaI AGTACT 1 cut(s) 1272
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 5 cut(s) 703, 781, 915, 1231, 1309
SduI GDGCHC 2 cut(s) 40, 1036
SfaNI GCATC 5 cut(s) 280, 495, 603, 1093, 1189
SfcI CTRYAG 2 cut(s) 294, 1088
SfuI TTCGAA 2 cut(s) 879, 1299
SinI GGWCC 3 cut(s) 257, 515, 734
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 2 cut(s) 251, 1070
SmoI CTYRAG 2 cut(s) 251, 1070
SphI GCATGC 2 cut(s) 644, 1079
Sse9I AATT 7 cut(s) 19, 113, 196, 210, 442, 775, 1191
SsiI CCGC 8 cut(s) 137, 407, 609, 629, 644, 1021, 1058, 1079
SspI AATATT 3 cut(s) 885, 995, 1304
StyD4I CCNGG 5 cut(s) 701, 779, 913, 1229, 1307
TaaI ACNGT 4 cut(s) 278, 346, 423, 1286
TaiI ACGT 2 cut(s) 10, 1266
TaqI TCGA 8 cut(s) 82, 243, 288, 327, 597, 879, 902, 1299
TasI AATT 7 cut(s) 19, 113, 196, 210, 442, 775, 1191
TatI WGTACW 1 cut(s) 1270
TfiI GAWTC 2 cut(s) 462, 1296
Tru1I TTAA 8 cut(s) 207, 213, 282, 446, 636, 758, 1194, 1260
Tru9I TTAA 8 cut(s) 207, 213, 282, 446, 636, 758, 1194, 1260
TscAI CASTG 1 cut(s) 1291
TseFI GTSAC 2 cut(s) 3, 1280
TseI GCWGC 4 cut(s) 149, 293, 415, 1087
Tsp45I GTSAC 2 cut(s) 3, 1280
TspDTI ATGAA 3 cut(s) 36, 102, 366
TspGWI ACGGA 3 cut(s) 193, 552, 854
TspRI CASTG 1 cut(s) 1291
Tth111I GACNNNGTC 1 cut(s) 1231
VneI GTGCAC 1 cut(s) 36
VpaK11BI GGWCC 3 cut(s) 257, 515, 734
VspI ATTAAT 3 cut(s) 213, 758, 1194
XapI RAATTY 1 cut(s) 196
XceI RCATGY 3 cut(s) 508, 644, 1079
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 67
ZrmI AGTACT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.