Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009726

Physical Location & Seq

Genomic Coordinates Reverse (-)

32379071 .. 32380216

Gene Structure 1146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1256100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1146 bp

ATGGGGCGTACTGCGCGGTCTGTCTCTCGGTTTTTCTCTCTGCGGAAGAAGAAACAAAAAGATTGGGCGTTTCCTGAAGAATTATGCCGTCATTTTACACATGCTGAGATCAGTGCAGCAACTCAATGTTTCGACGAGAGCTTATGTATTGCTAAGCGTAGCTTTGGCTCAGTATACAAAGGACATATTAAGGTCAATGGGGATGAGGACAGGAAAGACGTCGTTGCAATCAGGCGCATTTCCGAAGTTTCAGAACTGGTAATGCCTGATTTCAGGGCGGAGGTACAGCTACTCTGCCAGCTGCGCCATCCCAACCTCATCTCTCTCATCGGATTCTGCCAAGAAAATGGAGAGTGTATCATTGTCTACGATTACATGTCCAACGGTACCCTCTCTGATTACCTCCTTGATCCTTCCAACATTAAAAATAAAGATCATTTTCCCTTGACTTGGAAGCAAAGGCTGAAAATTTGTATTGGGGTGGCGCGTTCAATACACTACCTCCACGCAGGGGTTAAGCATGCTGTCATCCATCGAAACATAAAATGCTCCAACATTCTATTAGACCAAAACTTGGTGCCCAAGCTTTCTGACTTAGGGATTGCCAAGTTGGGTCCTTTTGCCTTGTCAAATGCATTAATCAAATTAAACTCGGAGGTGTCTGGTACAATAGGCTATCTTGATCCAGAGTATGCATTGTCTGGCAAGCTTACCGAAAAATCTGATGTCTTCTCATTTGGAATGGTGTTATTTGAAGTGCTGTGTGCCAAATCTTGTTCAACAGAGATGCCCGAATGCGTGGAAAGGGGTGAGACTTTCCCTGCCATGATTGATCCATTTCTGGTGGGAAAAGTAGCTCCAGATTGTTTGAGAAAATTTATGAACATCGCCGAGAGATGTGTGCGCTCTGCAGGAGCTGAACGGCCTACAATGGGTGAAGCTGAGGTGGAACTTGAGTGCGCATTGGAGTTGCAGGAGAGCGCAGATGCCGTCAAGCAGCTCAAGGAGTTGGGGACAACTGCAAGCAGTTCTCTTGCTCCTCCTCCTCCTGCCGCCCATGACATGGAGGACTATACCTACCAAGATATATCATTTTCTGAGATCAATGAAAATTTATCAAATATATATTTGCGAAAGTGGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

382

Amino Acids

42.66

Weight (kDa)

6.1

Isoelectric Point (pI)

51.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	51 - 314	3.2e-41	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	54 - 311	1.1e-31	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009726)

Species	Orthologous Gene IDs
malus_domestica	MD04G1237900.v1.1 MD04G1238200.v1.1 MD04G1238300.v1.1 MD04G1238400.v1.1 MD12G1256100.v1.1 MD12G1256200.v1.1 MD12G1256400.v1.1 MD12G1256500.v1.1
pyrus_communis	pycom02g03710 pycom04g21120 pycom04g21130 pycom12g23490 pycom12g23510 pycom17g22130
rosa_chinensis	RchiOBHm_Chr0c37g0502971 RchiOBHm_Chr6g0265111 RchiOBHm_Chr6g0265191
rosa_multiflora	Rmu_co8298979.1_g000001 Rmu_ssc0000359.1_g000070
rosa_samantha	Rh6AG136900 Rh6BG133800
rosa_wichuraiana	Rw6G011810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	222
Acc16I	TGCGCA	1 cut(s)	961
Acc65I	GGTACC	1 cut(s)	386
AccB1I	GGYRCC	2 cut(s)	386, 577
AccB7I	CCANNNNNTGG	2 cut(s)	574, 1063
AccI	GTMKAC	2 cut(s)	174, 366
AccII	CGCG	2 cut(s)	16, 487
AciI	CCGC	4 cut(s)	16, 43, 278, 1053
AclWI	GGATC	3 cut(s)	404, 677, 827
AcoI	YGGCCR	1 cut(s)	1139
AcsI	RAATTY	3 cut(s)	468, 875, 1111
AcuI	CTGAAG	1 cut(s)	96
AcyI	GRCGYC	1 cut(s)	219
AfaI	GTAC	4 cut(s)	10, 285, 388, 667
AfiI	CCNNNNNNNGG	5 cut(s)	450, 511, 574, 932, 1063
AflIII	ACRYGT	1 cut(s)	375
AgsI	TTSAA	3 cut(s)	492, 755, 780
Alw26I	GTCTC	2 cut(s)	28, 806
AlwI	GGATC	3 cut(s)	404, 677, 827
AlwNI	CAGNNNCTG	1 cut(s)	917
AoxI	GGCC	2 cut(s)	923, 1139
ApeKI	GCWGC	3 cut(s)	116, 301, 997
ApoI	RAATTY	3 cut(s)	468, 875, 1111
AseI	ATTAAT	1 cut(s)	638
Asp718I	GGTACC	1 cut(s)	386
AspLEI	GCGC	7 cut(s)	16, 237, 306, 487, 906, 962, 983
AspS9I	GGNCC	1 cut(s)	614
AsuHPI	GGTGA	2 cut(s)	821, 947
AvaII	GGWCC	1 cut(s)	614
BaeGI	GKGCMC	1 cut(s)	582
BanI	GGYRCC	2 cut(s)	386, 577
BbsI	GAAGAC	1 cut(s)	721
BbvCI	CCTCAGC	1 cut(s)	942
BbvI	GCAGC	3 cut(s)	128, 288, 1009
BccI	CCATC	2 cut(s)	315, 540
BceAI	ACGGC	4 cut(s)	72, 938, 974, 1126
BcoDI	GTCTC	2 cut(s)	28, 806
BfmI	CTRYAG	1 cut(s)	909
BisI	GCNGC	4 cut(s)	117, 302, 998, 1053
BlpI	GCTNAGC	1 cut(s)	153
BlsI	GCNGC	4 cut(s)	118, 303, 999, 1054
Bme18I	GGWCC	1 cut(s)	614
BmgT120I	GGNCC	1 cut(s)	614
BmiI	GGNNCC	3 cut(s)	388, 579, 615
BmsI	GCATC	2 cut(s)	777, 976
BpiI	GAAGAC	1 cut(s)	721
BpmI	CTGGAG	1 cut(s)	843
Bpu10I	CCTNAGC	1 cut(s)	942
Bpu1102I	GCTNAGC	1 cut(s)	153
BpuEI	CTTGAG	2 cut(s)	974, 986
BsaHI	GRCGYC	1 cut(s)	219
BsaXI	ACNNNNNCTCC	2 cut(s)	486, 516
Bsc4I	CCNNNNNNNGG	5 cut(s)	450, 511, 574, 932, 1063
Bse1I	ACTGG	1 cut(s)	261
BseGI	GGATG	3 cut(s)	208, 307, 528
BseLI	CCNNNNNNNGG	5 cut(s)	450, 511, 574, 932, 1063
BseMII	CTCAG	4 cut(s)	96, 183, 933, 1089
BseNI	ACTGG	1 cut(s)	261
BseRI	GAGGAG	3 cut(s)	1029, 1032, 1035
BseSI	GKGCMC	1 cut(s)	582
BseXI	GCAGC	3 cut(s)	128, 288, 1009
BsgI	GTGCAG	1 cut(s)	135
Bsh1236I	CGCG	2 cut(s)	16, 487
BshFI	GGCC	2 cut(s)	925, 1141
BshNI	GGYRCC	2 cut(s)	386, 577
BslFI	GGGAC	1 cut(s)	1027
BslI	CCNNNNNNNGG	5 cut(s)	450, 511, 574, 932, 1063
BsmAI	GTCTC	2 cut(s)	28, 806
BsmFI	GGGAC	1 cut(s)	1027
BsmI	GAATGC	1 cut(s)	800
BsnI	GGCC	2 cut(s)	925, 1141
Bsp1286I	GDGCHC	1 cut(s)	582
Bsp143I	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
Bsp1720I	GCTNAGC	1 cut(s)	153
BspACI	CCGC	4 cut(s)	16, 43, 278, 1053
BspANI	GGCC	2 cut(s)	925, 1141
BspCNI	CTCAG	4 cut(s)	97, 182, 934, 1090
BspFNI	CGCG	2 cut(s)	16, 487
BspLI	GGNNCC	3 cut(s)	388, 579, 615
BspMAI	CTGCAG	1 cut(s)	913
BspPI	GGATC	3 cut(s)	404, 677, 827
BspT107I	GGYRCC	2 cut(s)	386, 577
BsrI	ACTGG	1 cut(s)	261
BssMI	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
BssNAI	GTATAC	1 cut(s)	175
BssNI	GRCGYC	1 cut(s)	219
Bst1107I	GTATAC	1 cut(s)	175
Bst4CI	ACNGT	1 cut(s)	386
BstACI	GRCGYC	1 cut(s)	219
BstC8I	GCNNGC	4 cut(s)	299, 522, 707, 1024
BstDEI	CTNAG	6 cut(s)	105, 153, 169, 595, 942, 1098
BstF5I	GGATG	3 cut(s)	208, 307, 528
BstFNI	CGCG	2 cut(s)	16, 487
BstHHI	GCGC	7 cut(s)	16, 237, 306, 487, 906, 962, 983
BstKTI	GATC	6 cut(s)	111, 412, 436, 685, 835, 1104
BstMAI	GTCTC	2 cut(s)	28, 806
BstMBI	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
BstMWI	GCNNNNNNNGC	3 cut(s)	13, 303, 1138
BstNSI	RCATGY	3 cut(s)	104, 379, 524
BstSFI	CTRYAG	1 cut(s)	909
BstSLI	GKGCMC	1 cut(s)	582
BstUI	CGCG	2 cut(s)	16, 487
BstV1I	GCAGC	3 cut(s)	128, 288, 1009
BstV2I	GAAGAC	1 cut(s)	721
BstXI	CCANNNNNNTGG	1 cut(s)	347
BstZ17I	GTATAC	1 cut(s)	175
BsuRI	GGCC	2 cut(s)	925, 1141
BtgZI	GCGATG	1 cut(s)	871
BtsCI	GGATG	3 cut(s)	208, 307, 528
BtsIMutI	CAGTG	1 cut(s)	118
Cac8I	GCNNGC	4 cut(s)	299, 522, 707, 1024
CaiI	CAGNNNCTG	1 cut(s)	917
CfoI	GCGC	7 cut(s)	16, 237, 306, 487, 906, 962, 983
Cfr13I	GGNCC	1 cut(s)	614
Csp6I	GTAC	4 cut(s)	9, 284, 387, 666
CviAII	CATG	6 cut(s)	101, 376, 521, 826, 1058, 1063
CviQI	GTAC	4 cut(s)	9, 284, 387, 666
DdeI	CTNAG	6 cut(s)	105, 153, 169, 595, 942, 1098
DpnI	GATC	6 cut(s)	110, 411, 435, 684, 834, 1103
DpnII	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
EaeI	YGGCCR	1 cut(s)	1139
EciI	GGCGGA	1 cut(s)	293
Eco47I	GGWCC	1 cut(s)	614
Eco57I	CTGAAG	1 cut(s)	96
EcoO109I	RGGNCCY	1 cut(s)	614
EcoT22I	ATGCAT	2 cut(s)	637, 697
FaeI	CATG	6 cut(s)	104, 379, 524, 829, 1061, 1066
FalI	AAGNNNNNCTT	2 cut(s)	146, 178
FaqI	GGGAC	1 cut(s)	1027
FatI	CATG	6 cut(s)	100, 375, 520, 825, 1057, 1062
FblI	GTMKAC	2 cut(s)	174, 366
Fnu4HI	GCNGC	4 cut(s)	117, 302, 998, 1053
FokI	GGATG	3 cut(s)	215, 294, 515
Fsp4HI	GCNGC	4 cut(s)	117, 302, 998, 1053
FspAI	RTGCGCAY	1 cut(s)	961
FspI	TGCGCA	1 cut(s)	961
GlaI	GCGC	7 cut(s)	15, 236, 305, 486, 905, 961, 982
GluI	GCNGC	4 cut(s)	117, 302, 998, 1053
GsuI	CTGGAG	1 cut(s)	843
HaeIII	GGCC	2 cut(s)	925, 1141
HhaI	GCGC	7 cut(s)	16, 237, 306, 487, 906, 962, 983
Hin1I	GRCGYC	1 cut(s)	219
Hin1II	CATG	6 cut(s)	104, 379, 524, 829, 1061, 1066
Hin6I	GCGC	7 cut(s)	14, 235, 304, 485, 904, 960, 981
HinP1I	GCGC	7 cut(s)	14, 235, 304, 485, 904, 960, 981
HindIII	AAGCTT	2 cut(s)	584, 707
HinfI	GANTC	1 cut(s)	333
HphI	GGTGA	2 cut(s)	821, 947
Hpy166II	GTNNAC	2 cut(s)	175, 367
Hpy188I	TCNGA	8 cut(s)	244, 253, 332, 397, 592, 655, 724, 1099
Hpy188III	TCNNGA	4 cut(s)	74, 680, 686, 860
Hpy8I	GTNNAC	2 cut(s)	175, 367
Hpy99I	CGWCG	2 cut(s)	137, 224
HpyAV	CCTTC	1 cut(s)	423
HpyCH4III	ACNGT	1 cut(s)	386
HpyCH4IV	ACGT	1 cut(s)	219
HpyCH4V	TGCA	7 cut(s)	116, 227, 635, 695, 911, 973, 1022
HpyF10VI	GCNNNNNNNGC	3 cut(s)	13, 303, 1138
HpyF3I	CTNAG	6 cut(s)	105, 153, 169, 595, 942, 1098
HpySE526I	ACGT	1 cut(s)	219
Hsp92I	GRCGYC	1 cut(s)	219
Hsp92II	CATG	6 cut(s)	104, 379, 524, 829, 1061, 1066
HspAI	GCGC	7 cut(s)	14, 235, 304, 485, 904, 960, 981
KpnI	GGTACC	1 cut(s)	390
Kzo9I	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
LmnI	GCTCC	4 cut(s)	554, 862, 914, 1042
Lsp1109I	GCAGC	3 cut(s)	128, 288, 1009
LweI	GCATC	2 cut(s)	777, 976
MaeII	ACGT	1 cut(s)	219
MalI	GATC	6 cut(s)	110, 411, 435, 684, 834, 1103
MboI	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
MboII	GAAGA	4 cut(s)	58, 61, 89, 721
MhlI	GDGCHC	1 cut(s)	582
MluCI	AATT	5 cut(s)	80, 468, 644, 875, 1111
MmeI	TCCRAC	3 cut(s)	405, 441, 576
Mph1103I	ATGCAT	2 cut(s)	637, 697
MseI	TTAA	5 cut(s)	189, 423, 516, 638, 647
MspA1I	CMGCKG	1 cut(s)	301
Mva1269I	GAATGC	1 cut(s)	800
MvnI	CGCG	2 cut(s)	16, 487
MwoI	GCNNNNNNNGC	3 cut(s)	13, 303, 1138
NdeII	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
NlaIII	CATG	6 cut(s)	104, 379, 524, 829, 1061, 1066
NlaIV	GGNNCC	3 cut(s)	388, 579, 615
NmeAIII	GCCGAG	1 cut(s)	916
NsbI	TGCGCA	1 cut(s)	961
NsiI	ATGCAT	2 cut(s)	637, 697
NspI	RCATGY	3 cut(s)	104, 379, 524
PaeI	GCATGC	1 cut(s)	524
PciI	ACATGT	1 cut(s)	375
PctI	GAATGC	1 cut(s)	800
PfeI	GAWTC	1 cut(s)	333
PflMI	CCANNNNNTGG	2 cut(s)	574, 1063
PkrI	GCNGC	4 cut(s)	118, 303, 999, 1054
PpuMI	RGGWCCY	1 cut(s)	614
PscI	ACATGT	1 cut(s)	375
PshBI	ATTAAT	1 cut(s)	638
Psp5II	RGGWCCY	1 cut(s)	614
PspN4I	GGNNCC	3 cut(s)	388, 579, 615
PspPI	GGNCC	1 cut(s)	614
PspPPI	RGGWCCY	1 cut(s)	614
PstI	CTGCAG	1 cut(s)	913
PstNI	CAGNNNCTG	1 cut(s)	917
PvuII	CAGCTG	1 cut(s)	301
RsaI	GTAC	4 cut(s)	10, 285, 388, 667
RsaNI	GTAC	4 cut(s)	9, 284, 387, 666
SaqAI	TTAA	5 cut(s)	189, 423, 516, 638, 647
SatI	GCNGC	4 cut(s)	117, 302, 998, 1053
Sau3AI	GATC	6 cut(s)	108, 409, 433, 682, 832, 1101
Sau96I	GGNCC	1 cut(s)	614
SduI	GDGCHC	1 cut(s)	582
SfaNI	GCATC	2 cut(s)	777, 976
SfcI	CTRYAG	1 cut(s)	909
SinI	GGWCC	1 cut(s)	614
SmlI	CTYRAG	2 cut(s)	953, 1001
SmoI	CTYRAG	2 cut(s)	953, 1001
SphI	GCATGC	1 cut(s)	524
Sse9I	AATT	5 cut(s)	80, 468, 644, 875, 1111
SsiI	CCGC	4 cut(s)	16, 43, 278, 1053
TaaI	ACNGT	1 cut(s)	386
TaiI	ACGT	1 cut(s)	222
TaqI	TCGA	2 cut(s)	132, 535
TasI	AATT	5 cut(s)	80, 468, 644, 875, 1111
TauI	GCSGC	1 cut(s)	1055
TfiI	GAWTC	1 cut(s)	333
Tru1I	TTAA	5 cut(s)	189, 423, 516, 638, 647
Tru9I	TTAA	5 cut(s)	189, 423, 516, 638, 647
TscAI	CASTG	1 cut(s)	118
TseI	GCWGC	3 cut(s)	116, 301, 997
TspDTI	ATGAA	2 cut(s)	896, 1122
TspRI	CASTG	1 cut(s)	118
Van91I	CCANNNNNTGG	2 cut(s)	574, 1063
VpaK11BI	GGWCC	1 cut(s)	614
VspI	ATTAAT	1 cut(s)	638
XapI	RAATTY	3 cut(s)	468, 875, 1111
XceI	RCATGY	3 cut(s)	104, 379, 524
XmiI	GTMKAC	2 cut(s)	174, 366
ZraI	GACGTC	1 cut(s)	220
Zsp2I	ATGCAT	2 cut(s)	637, 697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.