pycom04g21130

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009726
Physical Location & Seq
Forward (+)
22449846 .. 22450970
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21130.1

Sequence Viewer

Length: 1125 bp
ATGATGGGTTTCAGTACCACTGCAGAGTCCGCCTCATGTTTCTTCTTCTCTTCATGGAAGAAACTAGCGTCATGGGATAAAAGTAGAAAACGCAGACCAAACCAAGACTGGGCCTTTATGGAGGAATTATGCCGCCGATTTTCGCTAGCTGAGATCATAGCAGCCACTGAATGCTTCAACGACAAGTTCCATATTGGTAGCAGTGCCGTGAGCTCAGTCTATAAAGGGTACATCAACGGCGGGGCAACTGTTGTTGCAATCAAGCGATTTACAGGCAGCAGACAAGACTTCAGGAATGAGGTGCTGCTGTCCTGCCAGCTACGCCACCCCAACCTTCTCCCTGTTATTGGATTCTGTGAAGAAAAGGAGGAACTCATCCTTGTCTACGACTACATGGCCAAAGGTACCCTCTCTTCGAAGCTGTTCAAAAGAGCGGATCTTGATCCGCTGCCCTGGAGGCAGAGGCTGAACATATGTATCGGGATAGCACGCGCATTGCATTATCTCCATGCAGGGCTAAAACATGCTATTGTCCACCGCGACATGAAATGCTCCATCATACTATTGGATGAAAAGTTCGAAGCAAAGGTTTCAAACTTATTAACGTTCAAGATGGGTCCTCCTAGTTTGTCAAATAAATTCATACGAGTGACCTCTGATAGGATCGTGGGCACTTTCGGGTATATTGATCCCGAGTATATCCATTTGGAACAATTTACAGAAAAATCTGATGTCTACTCGTTTGGAATGGTGCTGCTGGAAGTGTTGACTGCCAAATGGGCAAAAGAGTTTCATACATACACAGGCAGGCCGGACACCTTATGCGATATAATGGACCCTTCTTTGAGGGGTAAAATAGCTCCTGATTGTTTGCAAAAATTTATGGATATCGTTTATAGATGTGTAAGTCCAACAGGAGCAGAGCGACCCTCAGTAGGCGAAGTGCAAATGGAACTCGAGTGCGCATTGGAGCTGCAAGACAGCGCAGATGGCAAGAAGGACGCTTCCCTTGGTCTTGCAGGTGACTATAGCTACCAAGGGATGTCAATTTGTGAAATTGATGATATTTTCTCATTTTTGTTTAGGCAGCTCACAACTTCGGATGTCAGCTCTTTGTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

375

Amino Acids

42.39

Weight (kDa)

6.28

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 61 - 315 5.3e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 64 - 319 6.9e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
Acc16I TGCGCA 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 1010
Acc65I GGTACC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 434
AccI GTMKAC 2 cut(s) 384, 735
AccII CGCG 2 cut(s) 492, 540
AciI CCGC 6 cut(s) 30, 133, 240, 434, 446, 538
AclI AACGTT 1 cut(s) 605
AclWI GGATC 4 cut(s) 437, 444, 671, 683
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 638, 878
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 3 cut(s) 16, 230, 406
AfiI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
AgsI TTSAA 5 cut(s) 178, 427, 594, 610, 1121
AjnI CCWGG 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 9 cut(s) 149, 213, 319, 421, 860, 973, 1032, 1090, 1110
AluI AGCT 9 cut(s) 149, 213, 319, 421, 860, 973, 1032, 1090, 1110
Alw21I GWGCWC 1 cut(s) 215
AlwI GGATC 4 cut(s) 437, 444, 671, 683
AlwNI CAGNNNCTG 2 cut(s) 167, 466
Ama87I CYCGRG 2 cut(s) 692, 956
AoxI GGCC 3 cut(s) 111, 396, 809
ApeKI GCWGC 7 cut(s) 161, 276, 304, 448, 754, 973, 1087
ApoI RAATTY 2 cut(s) 638, 878
Asp700I GAANNNNTTC 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 404
AspLEI GCGC 3 cut(s) 494, 965, 986
AspS9I GGNCC 3 cut(s) 111, 617, 835
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 2 cut(s) 416, 579
AsuNHI GCTAGC 1 cut(s) 145
AvaI CYCGRG 2 cut(s) 692, 956
AvaII GGWCC 2 cut(s) 617, 835
BaeGI GKGCMC 1 cut(s) 674
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BalI TGGCCA 1 cut(s) 398
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 215
BarI GAAGNNNNNNTAC 2 cut(s) 397, 429
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 7 cut(s) 173, 288, 291, 435, 741, 960, 1099
BccI CCATC 3 cut(s) 563, 607, 983
BceAI ACGGC 2 cut(s) 191, 253
BciT130I CCWGG 1 cut(s) 454
BfaI CTAG 3 cut(s) 65, 146, 624
BfmI CTRYAG 2 cut(s) 21, 1027
BfuAI ACCTGC 1 cut(s) 1010
BglI GCCNNNNNGGC 2 cut(s) 457, 779
BisI GCNGC 8 cut(s) 133, 162, 277, 305, 449, 755, 974, 1088
BlsI GCNGC 8 cut(s) 134, 163, 278, 306, 450, 756, 975, 1089
Bme1390I CCNGG 1 cut(s) 454
Bme18I GGWCC 2 cut(s) 617, 835
BmeT110I CYCGRG 2 cut(s) 692, 956
BmgT120I GGNCC 3 cut(s) 111, 617, 835
BmiI GGNNCC 3 cut(s) 406, 618, 837
BmrFI CCNGG 1 cut(s) 454
BmrI ACTGGG 1 cut(s) 118
BmtI GCTAGC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 118
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 914, 946
BpmI CTGGAG 1 cut(s) 475
Bpu14I TTCGAA 2 cut(s) 416, 579
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 452, 1009, 1036
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 452, 1009, 1036
BseGI GGATG 4 cut(s) 375, 574, 1047, 1108
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 3 cut(s) 141, 228, 945
BseNI ACTGG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 7 cut(s) 173, 288, 291, 435, 741, 960, 1099
Bsh1236I CGCG 2 cut(s) 492, 540
BshFI GGCC 3 cut(s) 113, 398, 811
BshNI GGYRCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 2 cut(s) 692, 956
BsiSI CCGG 1 cut(s) 812
BslI CCNNNNNNNGG 4 cut(s) 109, 347, 660, 935
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 3 cut(s) 113, 398, 811
BsoBI CYCGRG 2 cut(s) 692, 956
Bsp119I TTCGAA 2 cut(s) 416, 579
Bsp1286I GDGCHC 2 cut(s) 215, 674
Bsp143I GATC 5 cut(s) 153, 436, 442, 663, 688
BspACI CCGC 6 cut(s) 30, 133, 240, 434, 446, 538
BspANI GGCC 3 cut(s) 113, 398, 811
BspCNI CTCAG 3 cut(s) 142, 227, 944
BspFNI CGCG 2 cut(s) 492, 540
BspLI GGNNCC 3 cut(s) 406, 618, 837
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 1010
BspOI GCTAGC 1 cut(s) 149
BspPI GGATC 4 cut(s) 437, 444, 671, 683
BspT104I TTCGAA 2 cut(s) 416, 579
BspT107I GGYRCC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 494
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 452, 1009, 1036
BssMI GATC 5 cut(s) 153, 436, 442, 663, 688
BssT1I CCWWGG 2 cut(s) 1009, 1036
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 55, 418
BstBI TTCGAA 2 cut(s) 416, 579
BstC8I GCNNGC 4 cut(s) 147, 317, 490, 809
BstDEI CTNAG 3 cut(s) 150, 214, 931
BstENI CCTNNNNNAGG 1 cut(s) 658
BstF5I GGATG 4 cut(s) 375, 574, 1047, 1108
BstFNI CGCG 2 cut(s) 492, 540
BstHHI GCGC 3 cut(s) 494, 965, 986
BstKTI GATC 5 cut(s) 156, 439, 445, 666, 691
BstMBI GATC 5 cut(s) 153, 436, 442, 663, 688
BstMWI GCNNNNNNNGC 5 cut(s) 29, 321, 457, 779, 990
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 452
BstSFI CTRYAG 2 cut(s) 21, 1027
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 2 cut(s) 492, 540
BstV1I GCAGC 7 cut(s) 173, 288, 291, 435, 741, 960, 1099
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 3 cut(s) 113, 398, 811
BtsCI GGATG 4 cut(s) 375, 574, 1047, 1108
BtsI GCAGTG 2 cut(s) 18, 208
BtsIMutI CAGTG 3 cut(s) 18, 165, 208
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 4 cut(s) 147, 317, 490, 809
CaiI CAGNNNCTG 2 cut(s) 167, 466
CfoI GCGC 3 cut(s) 494, 965, 986
Cfr13I GGNCC 3 cut(s) 111, 617, 835
CseI GACGC 2 cut(s) 57, 1010
Csp6I GTAC 3 cut(s) 15, 229, 405
CviAII CATG 7 cut(s) 36, 54, 72, 394, 509, 524, 544
CviQI GTAC 3 cut(s) 15, 229, 405
DdeI CTNAG 3 cut(s) 150, 214, 931
DpnI GATC 5 cut(s) 155, 438, 444, 665, 690
DpnII GATC 5 cut(s) 153, 436, 442, 663, 688
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 55, 418
EarI CTCTTC 2 cut(s) 55, 418
EciI GGCGGA 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 1009, 1036
Eco24I GRGCYC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 2 cut(s) 617, 835
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 274
Eco88I CYCGRG 2 cut(s) 692, 956
EcoICRI GAGCTC 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 658
EcoO109I RGGNCCY 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 452
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 1009, 1036
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 2 cut(s) 1009, 1036
FaeI CATG 7 cut(s) 39, 57, 75, 397, 512, 527, 547
FatI CATG 7 cut(s) 35, 53, 71, 393, 508, 523, 543
FauI CCCGC 1 cut(s) 233
FauNDI CATATG 1 cut(s) 473
FblI GTMKAC 2 cut(s) 384, 735
Fnu4HI GCNGC 8 cut(s) 133, 162, 277, 305, 449, 755, 974, 1088
FokI GGATG 4 cut(s) 362, 581, 1054, 1115
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 8 cut(s) 133, 162, 277, 305, 449, 755, 974, 1088
FspAI RTGCGCAY 1 cut(s) 964
FspBI CTAG 3 cut(s) 65, 146, 624
FspI TGCGCA 1 cut(s) 964
GlaI GCGC 3 cut(s) 493, 964, 985
GluI GCNGC 8 cut(s) 133, 162, 277, 305, 449, 755, 974, 1088
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 3 cut(s) 113, 398, 811
HapII CCGG 1 cut(s) 812
HgaI GACGC 2 cut(s) 57, 1010
HhaI GCGC 3 cut(s) 494, 965, 986
Hin1II CATG 7 cut(s) 39, 57, 75, 397, 512, 527, 547
Hin6I GCGC 3 cut(s) 492, 963, 984
HinP1I GCGC 3 cut(s) 492, 963, 984
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 2 cut(s) 26, 351
HpaII CCGG 1 cut(s) 812
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 4 cut(s) 385, 535, 736, 768
Hpy188I TCNGA 3 cut(s) 658, 730, 1102
Hpy188III TCNNGA 6 cut(s) 292, 440, 481, 610, 692, 863
Hpy8I GTNNAC 4 cut(s) 385, 535, 736, 768
HpyAV CCTTC 3 cut(s) 344, 849, 991
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 8 cut(s) 23, 257, 499, 512, 874, 946, 976, 1019
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 321, 457, 779, 990
HpyF3I CTNAG 3 cut(s) 150, 214, 931
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 7 cut(s) 39, 57, 75, 397, 512, 527, 547
HspAI GCGC 3 cut(s) 492, 963, 984
KpnI GGTACC 1 cut(s) 408
Kzo9I GATC 5 cut(s) 153, 436, 442, 663, 688
LmnI GCTCC 4 cut(s) 557, 865, 917, 970
Lsp1109I GCAGC 7 cut(s) 173, 288, 291, 435, 741, 960, 1099
MaeI CTAG 3 cut(s) 65, 146, 624
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 649, 1022
MalI GATC 5 cut(s) 155, 438, 444, 665, 690
MbiI CCGCTC 1 cut(s) 434
MboI GATC 5 cut(s) 153, 436, 442, 663, 688
MboII GAAGA 6 cut(s) 34, 37, 42, 70, 371, 405
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 215, 674
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 935
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 1 cut(s) 398
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 1 cut(s) 812
MspR9I CCNGG 1 cut(s) 454
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 2 cut(s) 492, 540
MwoI GCNNNNNNNGC 5 cut(s) 29, 321, 457, 779, 990
NdeI CATATG 1 cut(s) 473
NdeII GATC 5 cut(s) 153, 436, 442, 663, 688
NheI GCTAGC 1 cut(s) 145
NlaIII CATG 7 cut(s) 39, 57, 75, 397, 512, 527, 547
NlaIV GGNNCC 3 cut(s) 406, 618, 837
NmuCI GTSAC 2 cut(s) 649, 1022
NsbI TGCGCA 1 cut(s) 964
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 2 cut(s) 416, 579
PaeR7I CTCGAG 1 cut(s) 956
PaqCI CACCTGC 1 cut(s) 1010
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 8 cut(s) 134, 163, 278, 306, 450, 756, 975, 1089
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 215
Psp1406I AACGTT 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 406, 618, 837
PspPI GGNCC 3 cut(s) 111, 617, 835
PspPPI RGGWCCY 1 cut(s) 617
PspXI VCTCGAGB 1 cut(s) 956
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 2 cut(s) 167, 466
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 3 cut(s) 16, 230, 406
RsaNI GTAC 3 cut(s) 15, 229, 405
RseI CAYNNNNRTG 1 cut(s) 647
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 8 cut(s) 133, 162, 277, 305, 449, 755, 974, 1088
Sau3AI GATC 5 cut(s) 153, 436, 442, 663, 688
Sau96I GGNCC 3 cut(s) 111, 617, 835
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 454
SduI GDGCHC 2 cut(s) 215, 674
SfcI CTRYAG 2 cut(s) 21, 1027
Sfr274I CTCGAG 1 cut(s) 956
SfuI TTCGAA 2 cut(s) 416, 579
SinI GGWCC 2 cut(s) 617, 835
SlaI CTCGAG 1 cut(s) 956
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
SsiI CCGC 6 cut(s) 30, 133, 240, 434, 446, 538
SspMI CTAG 3 cut(s) 65, 146, 624
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 452
StyI CCWWGG 2 cut(s) 1009, 1036
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 608
TaqI TCGA 3 cut(s) 416, 579, 957
TasI AATT 6 cut(s) 125, 638, 713, 878, 1047, 1056
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 3 cut(s) 25, 172, 208
TseFI GTSAC 2 cut(s) 649, 1022
TseI GCWGC 7 cut(s) 161, 276, 304, 448, 754, 973, 1087
Tsp45I GTSAC 2 cut(s) 649, 1022
TspDTI ATGAA 5 cut(s) 42, 560, 585, 631, 782
TspRI CASTG 3 cut(s) 25, 172, 208
VpaK11BI GGWCC 2 cut(s) 617, 835
XagI CCTNNNNNAGG 1 cut(s) 658
XapI RAATTY 2 cut(s) 638, 878
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 2 cut(s) 105, 562
XhoI CTCGAG 1 cut(s) 956
XmiI GTMKAC 2 cut(s) 384, 735
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 3 cut(s) 65, 146, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.