MD12G1256500.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009726
Physical Location & Seq
Forward (+)
32414137 .. 32418548
4412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1256500.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGGGCGTACTGCGCAGTCTGTTTCTCGTTTCTTCTCTCTTCGGAAGAAGAAACAAAATGATTGGGCGTTTCCAGAAGAATTATGCCGTCGTTTTACACTTGCTGAGATCAGTGCAGCAACTCAATGTTTCGACCAAACCTTATGTATTGGTGAGAGTGTCTTTGGCACAGTATACAAAGGGCGTATTAAGGTCGATGGGGATGCGGACAGGAAAGACGTCGTTGCAATCAAGCGCTTTTCCGGAGGTTCAGAACGGGGAATGCGTGAGTTCAGGGCGGAGGTACAGCTACTGTGCCAGCTGCGCCACCCCAACCTCATCTCTCTCGTCGGATTCTGCCAAGAAAATGGAGAGTGTATCATTGTCTACGACTACATGCCCAACGGAACCCTCTCTGATTACCTCCTTGATCCTTCCAATATTAAAAATAAAGATCATTTTCCCTTGACTTGGAAGCAAAGGCTGAAAATTTGTATTGGGGTGGCGCGTTCAATACACTACCTCCACGCAGGGGTTAAGCATGCTGTCATCCATCGAGACGTGAAATGCACCAACGTTCTTTTGGACCAAAACTTGGTGCCCAAGCTTTCTGACTTCGGGTTGTCCAAGATGGGTCCTCCTGTCGTGTCAAATGCATTAATCAAATTAAACTCGACGGTGGTCGGTACATTCGGGTATCTTGATCCAGAGTATGCATTGTCCGGGCAACTTACCGAAAAATCTGATGTCTTTTCATTTGGAATGGTGTTACTTGAAGTGTTGTGTGCCAAATCTGTTTCAAGAGAGATATCTGAATGTGTGCATAGGGGTGAGACTTTCCCTGCTATGATTGATCCATTTCTGATGGGAAAAGTAGCTCCAGATTGTTTGAGAAAATTTATGAACATCGCTGTGAGATGTGTGCGCCCTACAGGAGCTGAACGGCCCACGATGGGTGAAGTGCAGGTGGAACTCGAGTGCGCACTGGAGTTGCAGGAGAGGGCTGATGCCGTCAAGCACCTCAATGAGTTGAGGACAAGTGCAAGCACTTCTCTTGCTCCTCCTCTTCCTGCCCATGACATGGAGGACTATACCTACGAAAATATATCATTTTCTGAGATCAATGAAACTTTCTGTATATTAGTGAGCTTCATTAGTACTGATGAGGCAGCTTCTGTTGATAGTATCGCTAGCCTTGATCCTAACAGGGAAGCAATAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

401

Amino Acids

44.59

Weight (kDa)

5.91

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 49 - 317 4.7e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 49 - 314 4.3e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 934
AatII GACGTC 1 cut(s) 222
Acc16I TGCGCA 2 cut(s) 15, 961
Acc36I ACCTGC 1 cut(s) 934
AccB1I GGYRCC 1 cut(s) 577
AccB7I CCANNNNNTGG 2 cut(s) 574, 1060
AccI GTMKAC 2 cut(s) 174, 366
AccII CGCG 1 cut(s) 487
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 2 cut(s) 206, 278
AclI AACGTT 1 cut(s) 555
AclWI GGATC 4 cut(s) 404, 677, 827, 1172
AcsI RAATTY 2 cut(s) 468, 875
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 4 cut(s) 10, 285, 667, 1138
AfeI AGCGCT 1 cut(s) 236
AfiI CCNNNNNNNGG 5 cut(s) 450, 511, 574, 932, 1060
AgsI TTSAA 3 cut(s) 492, 755, 780
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 7 cut(s) 289, 301, 586, 857, 917, 1128, 1151
AluI AGCT 7 cut(s) 289, 301, 586, 857, 917, 1128, 1151
Alw26I GTCTC 2 cut(s) 531, 806
AlwI GGATC 4 cut(s) 404, 677, 827, 1172
AlwNI CAGNNNCTG 3 cut(s) 292, 917, 1154
Ama87I CYCGRG 1 cut(s) 953
Aor13HI TCCGGA 1 cut(s) 242
Aor51HI AGCGCT 1 cut(s) 236
AoxI GGCC 1 cut(s) 923
ApeKI GCWGC 3 cut(s) 116, 301, 1148
ApoI RAATTY 2 cut(s) 468, 875
AseI ATTAAT 1 cut(s) 638
AspLEI GCGC 6 cut(s) 16, 237, 306, 487, 906, 962
AspS9I GGNCC 3 cut(s) 565, 614, 924
AsuC2I CCSGG 1 cut(s) 703
AsuHPI GGTGA 3 cut(s) 164, 821, 947
AsuNHI GCTAGC 1 cut(s) 1169
AvaI CYCGRG 1 cut(s) 953
AvaII GGWCC 2 cut(s) 565, 614
BaeGI GKGCMC 1 cut(s) 582
BanI GGYRCC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 128, 288, 1160
BccI CCATC 5 cut(s) 191, 540, 604, 838, 925
BceAI ACGGC 3 cut(s) 72, 938, 974
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcnI CCSGG 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 531, 806
BfaI CTAG 1 cut(s) 1170
BfmI CTRYAG 1 cut(s) 909
BfoI RGCGCY 1 cut(s) 238
BfuAI ACCTGC 1 cut(s) 934
BisI GCNGC 3 cut(s) 117, 302, 1149
BlsI GCNGC 3 cut(s) 118, 303, 1150
BmcAI AGTACT 1 cut(s) 1138
Bme1390I CCNGG 1 cut(s) 703
Bme18I GGWCC 2 cut(s) 565, 614
BmeT110I CYCGRG 1 cut(s) 953
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 565, 614, 924
BmiI GGNNCC 3 cut(s) 388, 579, 615
BmrFI CCNGG 1 cut(s) 703
BmsI GCATC 2 cut(s) 193, 976
BmtI GCTAGC 1 cut(s) 1173
BoxI GACNNNNGTC 1 cut(s) 659
BpmI CTGGAG 2 cut(s) 843, 986
BpuMI CCSGG 1 cut(s) 703
BsaHI GRCGYC 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 5 cut(s) 450, 511, 574, 932, 1060
Bse1I ACTGG 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 242
BseGI GGATG 2 cut(s) 208, 528
BseLI CCNNNNNNNGG 5 cut(s) 450, 511, 574, 932, 1060
BseMII CTCAG 2 cut(s) 96, 1086
BseNI ACTGG 1 cut(s) 969
BseRI GAGGAG 2 cut(s) 1029, 1032
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 3 cut(s) 128, 288, 1160
BsgI GTGCAG 2 cut(s) 135, 962
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 925
BshNI GGYRCC 1 cut(s) 577
BsiHKCI CYCGRG 1 cut(s) 953
BsiSI CCGG 2 cut(s) 243, 702
BslI CCNNNNNNNGG 5 cut(s) 450, 511, 574, 932, 1060
BsmAI GTCTC 2 cut(s) 531, 806
BsmBI CGTCTC 1 cut(s) 531
BsmI GAATGC 1 cut(s) 267
BsnI GGCC 1 cut(s) 925
BsoBI CYCGRG 1 cut(s) 953
Bsp1286I GDGCHC 1 cut(s) 582
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
BspACI CCGC 2 cut(s) 206, 278
BspANI GGCC 1 cut(s) 925
BspCNI CTCAG 2 cut(s) 97, 1087
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 3 cut(s) 388, 579, 615
BspMI ACCTGC 1 cut(s) 934
BspOI GCTAGC 1 cut(s) 1173
BspPI GGATC 4 cut(s) 404, 677, 827, 1172
BspT107I GGYRCC 1 cut(s) 577
BsrI ACTGG 1 cut(s) 969
BssMI GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
BssNAI GTATAC 1 cut(s) 175
BssNI GRCGYC 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 175
Bst4CI ACNGT 3 cut(s) 172, 294, 658
Bst6I CTCTTC 2 cut(s) 45, 1050
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 4 cut(s) 299, 522, 1024, 1171
BstDEI CTNAG 2 cut(s) 105, 1095
BstF5I GGATG 2 cut(s) 208, 528
BstFNI CGCG 1 cut(s) 487
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 6 cut(s) 16, 237, 306, 487, 906, 962
BstKTI GATC 7 cut(s) 111, 412, 436, 685, 835, 1101, 1180
BstMAI GTCTC 2 cut(s) 531, 806
BstMBI GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
BstMWI GCNNNNNNNGC 2 cut(s) 13, 303
BstNSI RCATGY 2 cut(s) 379, 524
BstPAI GACNNNNGTC 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 701
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 1 cut(s) 582
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 3 cut(s) 128, 288, 1160
BstXI CCANNNNNNTGG 1 cut(s) 347
BstZ17I GTATAC 1 cut(s) 175
BsuRI GGCC 1 cut(s) 925
BtgZI GCGATG 1 cut(s) 871
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 2 cut(s) 208, 528
BtsIMutI CAGTG 2 cut(s) 118, 962
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 4 cut(s) 299, 522, 1024, 1171
CaiI CAGNNNCTG 3 cut(s) 292, 917, 1154
CfoI GCGC 6 cut(s) 16, 237, 306, 487, 906, 962
Cfr13I GGNCC 3 cut(s) 565, 614, 924
Csp6I GTAC 4 cut(s) 9, 284, 666, 1137
CviAII CATG 4 cut(s) 376, 521, 1055, 1060
CviQI GTAC 4 cut(s) 9, 284, 666, 1137
DdeI CTNAG 2 cut(s) 105, 1095
DpnI GATC 7 cut(s) 110, 411, 435, 684, 834, 1100, 1179
DpnII GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
Eam1104I CTCTTC 2 cut(s) 45, 1050
EarI CTCTTC 2 cut(s) 45, 1050
EciI GGCGGA 1 cut(s) 293
Eco32I GATATC 1 cut(s) 789
Eco47I GGWCC 2 cut(s) 565, 614
Eco47III AGCGCT 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 953
EcoO109I RGGNCCY 1 cut(s) 614
EcoRV GATATC 1 cut(s) 789
EcoT22I ATGCAT 2 cut(s) 637, 697
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 4 cut(s) 379, 524, 1058, 1063
FatI CATG 4 cut(s) 375, 520, 1054, 1059
FblI GTMKAC 2 cut(s) 174, 366
Fnu4HI GCNGC 3 cut(s) 117, 302, 1149
FokI GGATG 2 cut(s) 215, 515
Fsp4HI GCNGC 3 cut(s) 117, 302, 1149
FspAI RTGCGCAY 1 cut(s) 961
FspBI CTAG 1 cut(s) 1170
FspI TGCGCA 2 cut(s) 15, 961
GlaI GCGC 6 cut(s) 15, 236, 305, 486, 905, 961
GluI GCNGC 3 cut(s) 117, 302, 1149
GsuI CTGGAG 2 cut(s) 843, 986
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 1 cut(s) 925
HapII CCGG 2 cut(s) 243, 702
HhaI GCGC 6 cut(s) 16, 237, 306, 487, 906, 962
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 379, 524, 1058, 1063
Hin6I GCGC 6 cut(s) 14, 235, 304, 485, 904, 960
HinP1I GCGC 6 cut(s) 14, 235, 304, 485, 904, 960
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 1 cut(s) 333
HpaII CCGG 2 cut(s) 243, 702
HphI GGTGA 3 cut(s) 164, 821, 947
Hpy166II GTNNAC 2 cut(s) 175, 367
Hpy188I TCNGA 9 cut(s) 45, 253, 332, 397, 592, 724, 793, 843, 1096
Hpy188III TCNNGA 7 cut(s) 74, 243, 536, 680, 686, 780, 860
Hpy8I GTNNAC 2 cut(s) 175, 367
Hpy99I CGWCG 4 cut(s) 93, 224, 332, 658
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 3 cut(s) 172, 294, 658
HpyCH4IV ACGT 3 cut(s) 219, 540, 555
HpyCH4V TGCA 9 cut(s) 116, 227, 549, 635, 695, 802, 943, 973, 1022
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 303
HpyF3I CTNAG 2 cut(s) 105, 1095
HpySE526I ACGT 3 cut(s) 219, 540, 555
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 379, 524, 1058, 1063
HspAI GCGC 6 cut(s) 14, 235, 304, 485, 904, 960
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
LmnI GCTCC 3 cut(s) 862, 914, 1042
Lsp1109I GCAGC 3 cut(s) 128, 288, 1160
LweI GCATC 2 cut(s) 193, 976
MaeI CTAG 1 cut(s) 1170
MaeII ACGT 3 cut(s) 219, 540, 555
MaeIII GTNAC 1 cut(s) 747
MalI GATC 7 cut(s) 110, 411, 435, 684, 834, 1100, 1179
MboI GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
MboII GAAGA 6 cut(s) 25, 32, 58, 61, 89, 1037
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 4 cut(s) 80, 468, 644, 875
MmeI TCCRAC 1 cut(s) 310
Mph1103I ATGCAT 2 cut(s) 637, 697
MroI TCCGGA 1 cut(s) 242
MseI TTAA 5 cut(s) 189, 423, 516, 638, 647
MslI CAYNNNNRTG 3 cut(s) 807, 890, 1002
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 2 cut(s) 243, 702
MspR9I CCNGG 1 cut(s) 703
Mva1269I GAATGC 1 cut(s) 267
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 2 cut(s) 13, 303
NciI CCSGG 1 cut(s) 703
NdeII GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
NheI GCTAGC 1 cut(s) 1169
NlaIII CATG 4 cut(s) 379, 524, 1058, 1063
NlaIV GGNNCC 3 cut(s) 388, 579, 615
NsbI TGCGCA 2 cut(s) 15, 961
NsiI ATGCAT 2 cut(s) 637, 697
NspI RCATGY 2 cut(s) 379, 524
PaeI GCATGC 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 953
PaqCI CACCTGC 1 cut(s) 934
PcsI WCGNNNNNNNCGW 1 cut(s) 262
PctI GAATGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 333
PflMI CCANNNNNTGG 2 cut(s) 574, 1060
PkrI GCNGC 3 cut(s) 118, 303, 1150
PpuMI RGGWCCY 1 cut(s) 614
PshAI GACNNNNGTC 1 cut(s) 659
PshBI ATTAAT 1 cut(s) 638
Psp1406I AACGTT 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 614
PspN4I GGNNCC 3 cut(s) 388, 579, 615
PspPI GGNCC 3 cut(s) 565, 614, 924
PspPPI RGGWCCY 1 cut(s) 614
PspXI VCTCGAGB 1 cut(s) 953
PstNI CAGNNNCTG 3 cut(s) 292, 917, 1154
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 4 cut(s) 10, 285, 667, 1138
RsaNI GTAC 4 cut(s) 9, 284, 666, 1137
RseI CAYNNNNRTG 3 cut(s) 807, 890, 1002
SaqAI TTAA 5 cut(s) 189, 423, 516, 638, 647
SatI GCNGC 3 cut(s) 117, 302, 1149
Sau3AI GATC 7 cut(s) 108, 409, 433, 682, 832, 1098, 1177
Sau96I GGNCC 3 cut(s) 565, 614, 924
ScaI AGTACT 1 cut(s) 1138
ScrFI CCNGG 1 cut(s) 703
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 2 cut(s) 193, 976
SfcI CTRYAG 1 cut(s) 909
Sfr274I CTCGAG 1 cut(s) 953
SinI GGWCC 2 cut(s) 565, 614
SlaI CTCGAG 1 cut(s) 953
SmiMI CAYNNNNRTG 3 cut(s) 807, 890, 1002
SmlI CTYRAG 1 cut(s) 953
SmoI CTYRAG 1 cut(s) 953
SphI GCATGC 1 cut(s) 524
Sse9I AATT 4 cut(s) 80, 468, 644, 875
SsiI CCGC 2 cut(s) 206, 278
SspI AATATT 1 cut(s) 421
SspMI CTAG 1 cut(s) 1170
StyD4I CCNGG 1 cut(s) 701
TaaI ACNGT 3 cut(s) 172, 294, 658
TaiI ACGT 3 cut(s) 222, 543, 558
TaqI TCGA 5 cut(s) 132, 195, 535, 653, 954
TasI AATT 4 cut(s) 80, 468, 644, 875
TatI WGTACW 1 cut(s) 1136
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 5 cut(s) 189, 423, 516, 638, 647
Tru9I TTAA 5 cut(s) 189, 423, 516, 638, 647
TscAI CASTG 2 cut(s) 118, 969
TseI GCWGC 3 cut(s) 116, 301, 1148
TspDTI ATGAA 4 cut(s) 723, 896, 1119, 1120
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 2 cut(s) 118, 969
Van91I CCANNNNNTGG 2 cut(s) 574, 1060
VpaK11BI GGWCC 2 cut(s) 565, 614
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 2 cut(s) 468, 875
XceI RCATGY 2 cut(s) 379, 524
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 953
XmiI GTMKAC 2 cut(s) 174, 366
XspI CTAG 1 cut(s) 1170
ZraI GACGTC 1 cut(s) 220
ZrmI AGTACT 1 cut(s) 1138
Zsp2I ATGCAT 2 cut(s) 637, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.