Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004836

Physical Location & Seq

Genomic Coordinates Forward (+)

5987786 .. 5989426

Gene Structure 1641 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1037200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 885 bp

ATGGCATCTTTGAGCCGGAGAGACGACGGGGATTCTCCACTTCCGGCGAAAGCTCCAACCTTTTTCAAGATTGTTTTGGACAAAGCTCTTCGAGATGGCAGGCTTGAAATTCCACCGGCGTTTGTAATGAAGAATGAAGATTGTCTGGCGGATTCCGTGTTCCTCAAGGATCCAACGGGGCGAGTTTGGCGAATAGAATTGACAAGGAGATGTGGCAAGGTGTGGTTGCAAAAGGGCTGGTCAGAATTTGCAAAATTTTACTCCCTGAACCATGGATACTTGTTGGTGTTTAGCCACGAAGGTGGTTTTTCACATTTCCTAGTGCGCATTTTTCAAAGGAATACGTTGGAGATTGATTATCCAATCAGTTCAAGCCATGATGGGGTACCCATGTTATGTGGTGGAATCCAGCTGCCGAAGAATGAAGAACATGGAGATAATGTTTCTGTCAAAATCTTGGATTCGCCTCGCCCAAAAACAAGAAGCGGTGGGGGCATGCATACGGATGAGGAAAAAACCACGAGGTTAAGTGATAGAGCCTTTTCGAGAGCTGTGAGTTTCAAATCTAGCAATCCGTTTCTCGTGGTCAAAATGCAGCCATCGTACATAGCAAGTCAGTTGAGACTATCTGGATCGTTTGTCAGGCGACATATCTGTGCAAGAGGCGTTTGCGACATGAACCTAGAGATCTCGAGCAGGAGAAGTTGGCCTGTCAAGTGCCGCGTCGGGGTATACAGGAGAAAACAATATGCAAGAGTCTATGACGGTTGGAAAGCATTTGCGCGAGAGAATCGTGTTAAAGTAGGGGATGCTTGTGTGTTTGAGTTGATTAATAAGGGTGGCACTCGCAGTGCTACATTCAAGGTTGGCGTATACCGAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

295

Amino Acids

33.33

Weight (kDa)

9.85

Isoelectric Point (pI)

56.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	27 - 114	1.5e-15	B3 DNA binding domain
B3	PF02362	193 - 282	2.9e-13	B3 DNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004836)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g13840 FvH4_1g14720 FvH4_2g29240 FvH4_2g29322 FvH4_2g29350 FvH4_3g34770 FvH4_7g25080
malus_domestica	MD05G1037200.v1.1
prunus_persica	Prupe.7G143200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0103581 RchiOBHm_Chr5g0062511
rosa_laevigata	RLG00000017246 RLG00000035562
rosa_multiflora	Rmu_co8088552.1_g000001 Rmu_sc0001530.1_g000008 Rmu_sc0001852.1_g000021 Rmu_sc0004988.1_g000026
rosa_roxburghii	Rroxscaffold_1G00018100 Rroxscaffold_2G00139510
rosa_rugosa	Rorug02G0115900 Rorug05G0350300 Rorug05G0350300
rosa_samantha	Rh2AG164200 Rh2BG171400 Rh2CG170900 Rh2DG169500 Rh5AG410300 Rh5BG424300 Rh5DG438600
rosa_wichuraiana	Rw2G012910 Rw5G038630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	326
Acc65I	GGTACC	1 cut(s)	385
AccB1I	GGYRCC	1 cut(s)	385
AccI	GTMKAC	2 cut(s)	732, 873
AccII	CGCG	2 cut(s)	723, 784
AciI	CCGC	3 cut(s)	149, 486, 721
AclWI	GGATC	3 cut(s)	164, 177, 640
AcsI	RAATTY	3 cut(s)	108, 245, 254
AfaI	GTAC	2 cut(s)	387, 605
AfiI	CCNNNNNNNGG	2 cut(s)	382, 727
AgsI	TTSAA	6 cut(s)	67, 107, 335, 372, 562, 862
AleI	CACNNNNGTG	1 cut(s)	300
AloI	GAACNNNNNNTCC	2 cut(s)	143, 175
AluBI	AGCT	5 cut(s)	53, 86, 412, 551, 881
AluI	AGCT	5 cut(s)	53, 86, 412, 551, 881
Alw26I	GTCTC	2 cut(s)	15, 616
AlwI	GGATC	3 cut(s)	164, 177, 640
Ama87I	CYCGRG	1 cut(s)	691
AoxI	GGCC	1 cut(s)	707
ApeKI	GCWGC	2 cut(s)	412, 595
ApoI	RAATTY	3 cut(s)	108, 245, 254
AseI	ATTAAT	1 cut(s)	831
Asp718I	GGTACC	1 cut(s)	385
AspLEI	GCGC	2 cut(s)	327, 784
AvaI	CYCGRG	1 cut(s)	691
BaeI	ACNNNNGTAYC	2 cut(s)	377, 410
BamHI	GGATCC	1 cut(s)	169
BanI	GGYRCC	1 cut(s)	385
BauI	CACGAG	2 cut(s)	520, 581
BbvI	GCAGC	2 cut(s)	399, 607
BccI	CCATC	3 cut(s)	89, 374, 607
BciVI	GTATCC	1 cut(s)	269
BcoDI	GTCTC	2 cut(s)	15, 616
BfaI	CTAG	3 cut(s)	320, 567, 683
BfuI	GTATCC	1 cut(s)	269
BglII	AGATCT	1 cut(s)	687
BisI	GCNGC	3 cut(s)	413, 596, 721
BlsI	GCNGC	3 cut(s)	414, 597, 722
BmeT110I	CYCGRG	1 cut(s)	691
BmiI	GGNNCC	2 cut(s)	171, 387
BmsI	GCATC	2 cut(s)	14, 799
BpuEI	CTTGAG	1 cut(s)	149
BsaJI	CCNNGG	1 cut(s)	271
BsaXI	ACNNNNNCTCC	2 cut(s)	426, 456
Bsc4I	CCNNNNNNNGG	2 cut(s)	382, 727
Bse118I	RCCGGY	1 cut(s)	115
BseDI	CCNNGG	1 cut(s)	271
BseGI	GGATG	2 cut(s)	511, 814
BseLI	CCNNNNNNNGG	2 cut(s)	382, 727
BseXI	GCAGC	2 cut(s)	399, 607
Bsh1236I	CGCG	2 cut(s)	723, 784
BshFI	GGCC	1 cut(s)	709
BshNI	GGYRCC	1 cut(s)	385
BsiHKCI	CYCGRG	1 cut(s)	691
BsiSI	CCGG	3 cut(s)	16, 44, 116
BslI	CCNNNNNNNGG	2 cut(s)	382, 727
BsmAI	GTCTC	2 cut(s)	15, 616
BsmBI	CGTCTC	1 cut(s)	15
BsnI	GGCC	1 cut(s)	709
BsoBI	CYCGRG	1 cut(s)	691
Bsp143I	GATC	3 cut(s)	169, 632, 687
Bsp19I	CCATGG	1 cut(s)	271
BspACI	CCGC	3 cut(s)	149, 486, 721
BspANI	GGCC	1 cut(s)	709
BspFNI	CGCG	2 cut(s)	723, 784
BspLI	GGNNCC	2 cut(s)	171, 387
BspPI	GGATC	3 cut(s)	164, 177, 640
BspQI	GCTCTTC	1 cut(s)	93
BspT107I	GGYRCC	1 cut(s)	385
BsrFI	RCCGGY	1 cut(s)	115
BssAI	RCCGGY	1 cut(s)	115
BssECI	CCNNGG	1 cut(s)	271
BssMI	GATC	3 cut(s)	169, 632, 687
BssNAI	GTATAC	2 cut(s)	733, 874
BssSI	CACGAG	2 cut(s)	520, 581
BssT1I	CCWWGG	1 cut(s)	271
Bst1107I	GTATAC	2 cut(s)	733, 874
Bst2BI	CACGAG	2 cut(s)	520, 581
Bst4CI	ACNGT	1 cut(s)	767
Bst6I	CTCTTC	1 cut(s)	93
BstC8I	GCNNGC	2 cut(s)	101, 497
BstDSI	CCRYGG	1 cut(s)	271
BstF5I	GGATG	2 cut(s)	511, 814
BstFNI	CGCG	2 cut(s)	723, 784
BstHHI	GCGC	2 cut(s)	327, 784
BstKTI	GATC	3 cut(s)	172, 635, 690
BstMAI	GTCTC	2 cut(s)	15, 616
BstMBI	GATC	3 cut(s)	169, 632, 687
BstMWI	GCNNNNNNNGC	2 cut(s)	187, 492
BstNSI	RCATGY	1 cut(s)	499
BstUI	CGCG	2 cut(s)	723, 784
BstV1I	GCAGC	2 cut(s)	399, 607
BstX2I	RGATCY	2 cut(s)	169, 687
BstXI	CCANNNNNNTGG	1 cut(s)	302
BstYI	RGATCY	2 cut(s)	169, 687
BstZ17I	GTATAC	2 cut(s)	733, 874
BsuI	GTATCC	1 cut(s)	269
BsuRI	GGCC	1 cut(s)	709
BtgI	CCRYGG	1 cut(s)	271
BtsCI	GGATG	2 cut(s)	511, 814
BtsI	GCAGTG	1 cut(s)	856
BtsIMutI	CAGTG	1 cut(s)	856
Cac8I	GCNNGC	2 cut(s)	101, 497
CfoI	GCGC	2 cut(s)	327, 784
Cfr10I	RCCGGY	1 cut(s)	115
CseI	GACGC	1 cut(s)	712
Csp6I	GTAC	2 cut(s)	386, 604
CviAII	CATG	6 cut(s)	272, 377, 391, 431, 496, 676
CviQI	GTAC	2 cut(s)	386, 604
DpnI	GATC	3 cut(s)	171, 634, 689
DpnII	GATC	3 cut(s)	169, 632, 687
Eam1104I	CTCTTC	1 cut(s)	93
EarI	CTCTTC	1 cut(s)	93
EciI	GGCGGA	1 cut(s)	164
Eco130I	CCWWGG	1 cut(s)	271
Eco88I	CYCGRG	1 cut(s)	691
EcoT14I	CCWWGG	1 cut(s)	271
EcoT22I	ATGCAT	1 cut(s)	501
ErhI	CCWWGG	1 cut(s)	271
Esp3I	CGTCTC	1 cut(s)	15
FaeI	CATG	6 cut(s)	275, 380, 394, 434, 499, 679
FatI	CATG	6 cut(s)	271, 376, 390, 430, 495, 675
FblI	GTMKAC	2 cut(s)	732, 873
Fnu4HI	GCNGC	3 cut(s)	413, 596, 721
FokI	GGATG	2 cut(s)	518, 821
Fsp4HI	GCNGC	3 cut(s)	413, 596, 721
FspAI	RTGCGCAY	1 cut(s)	326
FspBI	CTAG	3 cut(s)	320, 567, 683
FspI	TGCGCA	1 cut(s)	326
GlaI	GCGC	2 cut(s)	326, 783
GluI	GCNGC	3 cut(s)	413, 596, 721
HaeIII	GGCC	1 cut(s)	709
HapII	CCGG	3 cut(s)	16, 44, 116
HgaI	GACGC	1 cut(s)	712
HhaI	GCGC	2 cut(s)	327, 784
Hin1II	CATG	6 cut(s)	275, 380, 394, 434, 499, 679
Hin6I	GCGC	2 cut(s)	325, 782
HinP1I	GCGC	2 cut(s)	325, 782
HinfI	GANTC	6 cut(s)	32, 152, 405, 461, 756, 790
HpaII	CCGG	3 cut(s)	16, 44, 116
Hpy166II	GTNNAC	2 cut(s)	733, 874
Hpy188I	TCNGA	1 cut(s)	244
Hpy188III	TCNNGA	5 cut(s)	67, 92, 546, 630, 691
Hpy8I	GTNNAC	2 cut(s)	733, 874
Hpy99I	CGWCG	2 cut(s)	29, 728
HpyAV	CCTTC	1 cut(s)	293
HpyCH4III	ACNGT	1 cut(s)	767
HpyCH4IV	ACGT	1 cut(s)	344
HpyCH4V	TGCA	6 cut(s)	229, 251, 499, 595, 659, 752
HpyF10VI	GCNNNNNNNGC	2 cut(s)	187, 492
HpySE526I	ACGT	1 cut(s)	344
Hsp92II	CATG	6 cut(s)	275, 380, 394, 434, 499, 679
HspAI	GCGC	2 cut(s)	325, 782
KpnI	GGTACC	1 cut(s)	389
Kzo9I	GATC	3 cut(s)	169, 632, 687
LguI	GCTCTTC	1 cut(s)	93
LmnI	GCTCC	1 cut(s)	58
Lsp1109I	GCAGC	2 cut(s)	399, 607
LweI	GCATC	2 cut(s)	14, 799
MaeI	CTAG	3 cut(s)	320, 567, 683
MaeII	ACGT	1 cut(s)	344
MalI	GATC	3 cut(s)	171, 634, 689
MboI	GATC	3 cut(s)	169, 632, 687
MboII	GAAGA	5 cut(s)	80, 142, 149, 430, 437
MflI	RGATCY	2 cut(s)	169, 687
MluCI	AATT	4 cut(s)	108, 197, 245, 254
MlyI	GAGTC	1 cut(s)	765
MmeI	TCCRAC	4 cut(s)	80, 197, 327, 749
MnlI	CCTC	5 cut(s)	173, 477, 502, 516, 656
Mph1103I	ATGCAT	1 cut(s)	501
MseI	TTAA	4 cut(s)	527, 798, 831, 883
MslI	CAYNNNNRTG	3 cut(s)	300, 504, 654
MspA1I	CMGCKG	1 cut(s)	412
MspI	CCGG	3 cut(s)	16, 44, 116
MvnI	CGCG	2 cut(s)	723, 784
MwoI	GCNNNNNNNGC	2 cut(s)	187, 492
NcoI	CCATGG	1 cut(s)	271
NdeII	GATC	3 cut(s)	169, 632, 687
NlaIII	CATG	6 cut(s)	275, 380, 394, 434, 499, 679
NlaIV	GGNNCC	2 cut(s)	171, 387
NsbI	TGCGCA	1 cut(s)	326
NsiI	ATGCAT	1 cut(s)	501
NspI	RCATGY	1 cut(s)	499
OliI	CACNNNNGTG	1 cut(s)	300
PaeI	GCATGC	1 cut(s)	499
PaeR7I	CTCGAG	1 cut(s)	691
PciSI	GCTCTTC	1 cut(s)	93
PfeI	GAWTC	5 cut(s)	32, 152, 405, 461, 790
PkrI	GCNGC	3 cut(s)	414, 597, 722
PleI	GAGTC	1 cut(s)	764
PpsI	GAGTC	1 cut(s)	764
PshBI	ATTAAT	1 cut(s)	831
PspN4I	GGNNCC	2 cut(s)	171, 387
PsrI	GAACNNNNNNTAC	2 cut(s)	260, 292
PsuI	RGATCY	2 cut(s)	169, 687
PvuII	CAGCTG	1 cut(s)	412
RsaI	GTAC	2 cut(s)	387, 605
RsaNI	GTAC	2 cut(s)	386, 604
RseI	CAYNNNNRTG	3 cut(s)	300, 504, 654
SapI	GCTCTTC	1 cut(s)	93
SaqAI	TTAA	4 cut(s)	527, 798, 831, 883
SatI	GCNGC	3 cut(s)	413, 596, 721
Sau3AI	GATC	3 cut(s)	169, 632, 687
SchI	GAGTC	1 cut(s)	765
SfaNI	GCATC	2 cut(s)	14, 799
Sfr274I	CTCGAG	1 cut(s)	691
SgrAI	CRCCGGYG	1 cut(s)	115
SlaI	CTCGAG	1 cut(s)	691
SmiMI	CAYNNNNRTG	3 cut(s)	300, 504, 654
SmlI	CTYRAG	2 cut(s)	164, 691
SmoI	CTYRAG	2 cut(s)	164, 691
SphI	GCATGC	1 cut(s)	499
Sse9I	AATT	4 cut(s)	108, 197, 245, 254
SsiI	CCGC	3 cut(s)	149, 486, 721
SspMI	CTAG	3 cut(s)	320, 567, 683
StyI	CCWWGG	1 cut(s)	271
TaaI	ACNGT	1 cut(s)	767
TaiI	ACGT	1 cut(s)	347
TaqI	TCGA	3 cut(s)	91, 545, 692
TasI	AATT	4 cut(s)	108, 197, 245, 254
TauI	GCSGC	1 cut(s)	723
TfiI	GAWTC	5 cut(s)	32, 152, 405, 461, 790
Tru1I	TTAA	4 cut(s)	527, 798, 831, 883
Tru9I	TTAA	4 cut(s)	527, 798, 831, 883
TscAI	CASTG	1 cut(s)	856
TseI	GCWGC	2 cut(s)	412, 595
TspDTI	ATGAA	4 cut(s)	143, 150, 438, 692
TspGWI	ACGGA	3 cut(s)	145, 518, 564
TspRI	CASTG	1 cut(s)	856
VspI	ATTAAT	1 cut(s)	831
XapI	RAATTY	3 cut(s)	108, 245, 254
XceI	RCATGY	1 cut(s)	499
XhoI	CTCGAG	1 cut(s)	691
XmiI	GTMKAC	2 cut(s)	732, 873
XspI	CTAG	3 cut(s)	320, 567, 683
Zsp2I	ATGCAT	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.