RchiOBHm_Chr2g0103581

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004836
Physical Location & Seq
Reverse (-)
15047838 .. 15049067
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47794

Sequence Viewer

Length: 795 bp
ATGGGAGAAACAGAAGAGCCTTCTCCTTTTGCCGCCAAATCTCCAAGCTTCTTCAAGATCGTTCTTGACAGTGCACTTCGAGATGGAAAGATTGAAATTCCAAAGGCATTTGTGATGAAGCATGGAAATTGTTTGGCAGAGTCAGTATTGCTTAAGGTTCCTAGCAGAGCAGAATGGCGAATAGGATTGACAAGGTGTGATGGTGAGGTTTGGTTAGAAAAGGGCTGGCCAGAATTCGCAAAGTTTTACTTCCTAGACCATGGATACTTATTAGTGTTCAGTTGTGAGGGTGATTATTCCAGTTTCCAAGTGCAAATTTTTACTAGGAATACTATGGAGATAGATTATCCCACGAATTCAAGCCTTGGCGAGGGGGAAGAACTTGAAGATGATGATGTTTCTGTTGAAATATTGGATGTGGTTCCCTCAAAGAAAAGAGGGAGAAGAGAATTGGCAAGCCTTTGGCCTCATAAGACGATGAGGACAAAATCAGGTGATGAAGCTGAAGTGAATTCCTACCCCAGAGGTAAAACTAAAGATCAAGGAGTGAAGCATCAAAGAGAAAAAAGAAGTTATGGTACTCCTAGTTCCTTAAAGAATTGTCAAGCGGAGGGGGGAAGCATGCCTACTGCATCAATATCTTTAGAGGCTAATGAGCATGATGTCGATACAAGAGATGGAGCGTTTCTGAGAGCTACAAGTTTCAAGTATAGTAATCCATATCTTGTAGTCAGAATGCAAAAATCATACCTATCAAGATATTTGGTAAGCTTTCTCAACTACCTGCTACAATAG

Protein Analysis

264

Amino Acids

29.95

Weight (kDa)

6.03

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 18 - 109 7.5e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 33, 608
AcoI YGGCCR 1 cut(s) 227
AcsI RAATTY 5 cut(s) 96, 233, 315, 355, 511
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 1 cut(s) 580
AfiI CCNNNNNNNGG 1 cut(s) 370
AflII CTTAAG 1 cut(s) 152
AgsI TTSAA 6 cut(s) 55, 95, 360, 386, 407, 706
AluBI AGCT 4 cut(s) 48, 503, 695, 771
AluI AGCT 4 cut(s) 48, 503, 695, 771
Alw21I GWGCWC 1 cut(s) 76
Alw44I GTGCAC 1 cut(s) 72
AoxI GGCC 2 cut(s) 227, 464
ApaLI GTGCAC 1 cut(s) 72
ApoI RAATTY 5 cut(s) 96, 233, 315, 355, 511
AsuHPI GGTGA 3 cut(s) 215, 302, 506
BaeGI GKGCMC 1 cut(s) 76
BalI TGGCCA 1 cut(s) 229
BarI GAAGNNNNNNTAC 4 cut(s) 562, 594, 610, 642
Bbv12I GWGCWC 1 cut(s) 76
BccI CCATC 3 cut(s) 77, 194, 671
BciVI GTATCC 1 cut(s) 257
BfaI CTAG 4 cut(s) 162, 254, 324, 585
BfrI CTTAAG 1 cut(s) 152
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
BmiI GGNNCC 2 cut(s) 159, 423
BmsI GCATC 2 cut(s) 562, 641
BsaJI CCNNGG 2 cut(s) 259, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 259, 364
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 1 cut(s) 300
BseSI GKGCMC 1 cut(s) 76
BshFI GGCC 2 cut(s) 229, 466
BsiHKAI GWGCWC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 370
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 2 cut(s) 229, 466
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 57, 538
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 2 cut(s) 33, 608
BspANI GGCC 2 cut(s) 229, 466
BspCNI CTCAG 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 159, 423
BspQI GCTCTTC 1 cut(s) 9
BspTI CTTAAG 1 cut(s) 152
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 259, 364
BssMI GATC 2 cut(s) 57, 538
BssT1I CCWWGG 2 cut(s) 259, 364
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 2 cut(s) 9, 439
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 227, 457, 623
BstDEI CTNAG 1 cut(s) 689
BstDSI CCRYGG 1 cut(s) 259
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 2 cut(s) 60, 541
BstMBI GATC 2 cut(s) 57, 538
BstNSI RCATGY 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 76
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 229, 466
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 3 cut(s) 227, 457, 623
Csp6I GTAC 1 cut(s) 579
CviAII CATG 4 cut(s) 122, 260, 622, 659
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 1 cut(s) 689
DpnI GATC 2 cut(s) 59, 540
DpnII GATC 2 cut(s) 57, 538
EaeI YGGCCR 1 cut(s) 227
Eam1104I CTCTTC 2 cut(s) 9, 439
EarI CTCTTC 2 cut(s) 9, 439
Eco130I CCWWGG 2 cut(s) 259, 364
Eco57I CTGAAG 1 cut(s) 525
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoRI GAATTC 3 cut(s) 233, 355, 511
EcoT14I CCWWGG 2 cut(s) 259, 364
ErhI CCWWGG 2 cut(s) 259, 364
FaeI CATG 4 cut(s) 125, 263, 625, 662
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 4 cut(s) 121, 259, 621, 658
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 4 cut(s) 162, 254, 324, 585
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 2 cut(s) 229, 466
Hin1II CATG 4 cut(s) 125, 263, 625, 662
HindIII AAGCTT 2 cut(s) 46, 769
HinfI GANTC 1 cut(s) 140
HphI GGTGA 3 cut(s) 215, 302, 506
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 2 cut(s) 690, 734
Hpy188III TCNNGA 4 cut(s) 55, 65, 80, 756
Hpy8I GTNNAC 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 4 cut(s) 74, 313, 632, 739
HpyF3I CTNAG 1 cut(s) 689
Hsp92II CATG 4 cut(s) 125, 263, 625, 662
Kzo9I GATC 2 cut(s) 57, 538
LguI GCTCTTC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 680
LpnPI CCDG 5 cut(s) 211, 243, 313, 477, 535
LweI GCATC 2 cut(s) 562, 641
MaeI CTAG 4 cut(s) 162, 254, 324, 585
MalI GATC 2 cut(s) 59, 540
MboI GATC 2 cut(s) 57, 538
MboII GAAGA 5 cut(s) 26, 43, 389, 398, 456
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 229
MluCI AATT 8 cut(s) 96, 127, 233, 315, 355, 449, 511, 598
MluNI TGGCCA 1 cut(s) 229
MlyI GAGTC 1 cut(s) 149
Mox20I TGGCCA 1 cut(s) 229
MscI TGGCCA 1 cut(s) 229
MseI TTAA 2 cut(s) 153, 593
Msp20I TGGCCA 1 cut(s) 229
MspCI CTTAAG 1 cut(s) 152
Mva1269I GAATGC 1 cut(s) 741
NcoI CCATGG 1 cut(s) 259
NdeII GATC 2 cut(s) 57, 538
NlaIII CATG 4 cut(s) 125, 263, 625, 662
NlaIV GGNNCC 2 cut(s) 159, 423
NspI RCATGY 1 cut(s) 625
PaeI GCATGC 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 9
PctI GAATGC 1 cut(s) 741
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 159, 423
PsrI GAACNNNNNNTAC 2 cut(s) 571, 603
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 9
SaqAI TTAA 2 cut(s) 153, 593
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 2 cut(s) 57, 538
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 2 cut(s) 562, 641
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SphI GCATGC 1 cut(s) 625
Sse9I AATT 8 cut(s) 96, 127, 233, 315, 355, 449, 511, 598
SsiI CCGC 2 cut(s) 33, 608
SspI AATATT 1 cut(s) 411
SspMI CTAG 4 cut(s) 162, 254, 324, 585
StyI CCWWGG 2 cut(s) 259, 364
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 2 cut(s) 79, 666
TasI AATT 8 cut(s) 96, 127, 233, 315, 355, 449, 511, 598
TauI GCSGC 1 cut(s) 35
Tru1I TTAA 2 cut(s) 153, 593
Tru9I TTAA 2 cut(s) 153, 593
TscAI CASTG 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 131, 513
TspRI CASTG 1 cut(s) 76
Vha464I CTTAAG 1 cut(s) 152
VneI GTGCAC 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 368
XapI RAATTY 5 cut(s) 96, 233, 315, 355, 511
XceI RCATGY 1 cut(s) 625
XspI CTAG 4 cut(s) 162, 254, 324, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.