MD05G1050500.v1.1

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006023
Physical Location & Seq
Forward (+)
8745680 .. 8748488
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050500.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGGCTTGCACGTTCTGACGTACTCTCCCCTTTCTTCCTCCAAGACCATAATCTTCAACAGCACCACCATCACACCCACTCTCTGCAACCTCCTCCGCCCTCCAAACCCTAAACCCACCTCTCTCTTCCGAACCCGTTTCACAACTTTACCCCCAAAACACATCGCAAGCGTCATACCCGCTGCTGGGTTTGCTGGGTCTTTGCACCGCAGCAAGCGGAGCTTTCGTGGCGGGGTAGTTGTAGCCATGGCTGCACCTGGGTCGACCCAGAAATCTGAGGAGGAGTGGCGGACCGTGCTCTCTCCCGAGCAGTTTCGGATTCTGAGGCTGAAAGGCACAGAGTATCCGGGCACAGGAGAATATGACAAGTTCTTTGAGGAGGGAATCTATACCTGTGCAGGATGCTCGACTCCTCTCTACAGATCCACAACCAAATTTAATTCGGGTTGTGGCTGGCCAGCTTTTTATGAGGGTCTTCCTGGGGCGATAAATTTTAACCCAGACCCAGATGGGATGAGGACTGAAATTACATGTGCGGCTTGTGGTGGACACCTGGGTCATGTATTCAAAGGAGAAGGTTTTCGAACACCCACAAATGAACGCCATTGCGTTAATAGTATTTCACTCAAGTTTGTCCCTCCCAGTTCTAGTCCATCTCAGTGA

Protein Analysis

221

Amino Acids

23.94

Weight (kDa)

9.26

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SelR PF01641 100 - 213 1.8e-43 SelR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 555
AccI GTMKAC 1 cut(s) 263
AciI CCGC 7 cut(s) 97, 180, 208, 217, 231, 289, 536
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 2 cut(s) 434, 490
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 3 cut(s) 185, 186, 353
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 2 cut(s) 58, 568
AjnI CCWGG 3 cut(s) 256, 478, 552
AluBI AGCT 2 cut(s) 222, 461
AluI AGCT 2 cut(s) 222, 461
Alw21I GWGCWC 1 cut(s) 300
AlwI GGATC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 1 cut(s) 455
ApeKI GCWGC 3 cut(s) 182, 210, 251
ApoI RAATTY 2 cut(s) 434, 490
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 1 cut(s) 579
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 1 cut(s) 348
AsuII TTCGAA 1 cut(s) 583
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 169, 222, 238
BccI CCATC 3 cut(s) 77, 503, 661
BcgI CGANNNNNNTGC 4 cut(s) 243, 277, 387, 421
BciT130I CCWGG 3 cut(s) 258, 480, 554
BciVI GTATCC 1 cut(s) 354
BcnI CCSGG 1 cut(s) 348
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 4 cut(s) 183, 211, 252, 537
BlsI GCNGC 4 cut(s) 184, 212, 253, 538
Bme1390I CCNGG 4 cut(s) 258, 348, 480, 554
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 291
BmrFI CCNGG 4 cut(s) 258, 348, 480, 554
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 1 cut(s) 392
BmuI ACTGGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 348
BsaJI CCNNGG 4 cut(s) 246, 257, 479, 553
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 211, 241
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 186, 353
Bse1I ACTGG 1 cut(s) 642
Bse3DI GCAATG 1 cut(s) 604
BseBI CCWGG 3 cut(s) 258, 480, 554
BseDI CCNNGG 4 cut(s) 246, 257, 479, 553
BseGI GGATG 2 cut(s) 407, 519
BseLI CCNNNNNNNGG 3 cut(s) 185, 186, 353
BseMI GCAATG 1 cut(s) 604
BseMII CTCAG 2 cut(s) 267, 314
BseNI ACTGG 1 cut(s) 642
BseRI GAGGAG 5 cut(s) 83, 293, 296, 392, 402
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 3 cut(s) 169, 222, 238
BseYI CCCAGC 2 cut(s) 185, 194
BsgI GTGCAG 2 cut(s) 237, 417
BshFI GGCC 1 cut(s) 457
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 3 cut(s) 185, 186, 353
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 583
Bsp1286I GDGCHC 2 cut(s) 300, 353
Bsp143I GATC 1 cut(s) 422
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 7 cut(s) 97, 180, 208, 217, 231, 289, 536
BspANI GGCC 1 cut(s) 457
BspCNI CTCAG 2 cut(s) 268, 315
BspPI GGATC 1 cut(s) 417
BspT104I TTCGAA 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 604
BsrI ACTGG 1 cut(s) 642
BssECI CCNNGG 4 cut(s) 246, 257, 479, 553
BssMI GATC 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 258, 480, 554
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 5 cut(s) 8, 169, 215, 455, 459
BstDEI CTNAG 3 cut(s) 276, 323, 657
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 407, 519
BstKTI GATC 1 cut(s) 425
BstMBI GATC 1 cut(s) 422
BstMWI GCNNNNNNNGC 5 cut(s) 191, 219, 228, 251, 295
BstNI CCWGG 3 cut(s) 258, 480, 554
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 4 cut(s) 256, 346, 478, 552
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 169, 222, 238
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 1 cut(s) 457
BtgI CCRYGG 1 cut(s) 246
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 2 cut(s) 407, 519
Cac8I GCNNGC 5 cut(s) 8, 169, 215, 455, 459
Cfr13I GGNCC 1 cut(s) 291
CpoI CGGWCCG 1 cut(s) 291
CseI GACGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 22
CspI CGGWCCG 1 cut(s) 291
CviAII CATG 3 cut(s) 247, 531, 560
CviJI RGCY 9 cut(s) 6, 222, 245, 251, 328, 453, 457, 461, 539
CviKI_1 RGCY 9 cut(s) 6, 222, 245, 251, 328, 453, 457, 461, 539
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 3 cut(s) 276, 323, 657
DpnI GATC 1 cut(s) 424
DpnII GATC 1 cut(s) 422
DrdI GACNNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 2 cut(s) 86, 304
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 305
EcoRII CCWGG 3 cut(s) 256, 478, 552
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 3 cut(s) 250, 534, 563
FaiI YATR 8 cut(s) 50, 176, 248, 363, 390, 468, 532, 561
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 620
FatI CATG 3 cut(s) 246, 530, 559
FauI CCCGC 2 cut(s) 187, 224
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 4 cut(s) 183, 211, 252, 537
FokI GGATG 2 cut(s) 414, 526
Fsp4HI GCNGC 4 cut(s) 183, 211, 252, 537
FspBI CTAG 1 cut(s) 648
GluI GCNGC 4 cut(s) 183, 211, 252, 537
GsaI CCCAGC 2 cut(s) 189, 198
HaeIII GGCC 1 cut(s) 457
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 160
Hin1II CATG 3 cut(s) 250, 534, 563
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 3 cut(s) 319, 384, 409
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 2 cut(s) 264, 548
Hpy188I TCNGA 5 cut(s) 18, 131, 277, 318, 324
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 2 cut(s) 264, 548
HpyAV CCTTC 1 cut(s) 569
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 12, 20
HpyCH4V TGCA 5 cut(s) 10, 87, 205, 254, 398
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 219, 228, 251, 295
HpyF3I CTNAG 3 cut(s) 276, 323, 657
HpySE526I ACGT 2 cut(s) 12, 20
Hsp92II CATG 3 cut(s) 250, 534, 563
Kzo9I GATC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 169, 222, 238
LweI GCATC 1 cut(s) 392
MaeI CTAG 1 cut(s) 648
MaeII ACGT 2 cut(s) 12, 20
MalI GATC 1 cut(s) 424
MboI GATC 1 cut(s) 422
MboII GAAGA 4 cut(s) 27, 46, 118, 467
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 2 cut(s) 300, 353
MlsI TGGCCA 1 cut(s) 457
MluCI AATT 4 cut(s) 434, 439, 490, 525
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 1 cut(s) 403
Mox20I TGGCCA 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 1 cut(s) 457
MseI TTAA 3 cut(s) 438, 495, 612
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 4 cut(s) 258, 348, 480, 554
MvaI CCWGG 3 cut(s) 258, 480, 554
MwoI GCNNNNNNNGC 5 cut(s) 191, 219, 228, 251, 295
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 1 cut(s) 246
NdeII GATC 1 cut(s) 422
NlaIII CATG 3 cut(s) 250, 534, 563
NspI RCATGY 1 cut(s) 534
NspV TTCGAA 1 cut(s) 583
PciI ACATGT 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 319, 384
PkrI GCNGC 4 cut(s) 184, 212, 253, 538
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PscI ACATGT 1 cut(s) 530
Psp6I CCWGG 3 cut(s) 256, 478, 552
PspFI CCCAGC 2 cut(s) 185, 194
PspGI CCWGG 3 cut(s) 256, 478, 552
PspPI GGNCC 1 cut(s) 291
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 658
Rsr2I CGGWCCG 1 cut(s) 291
RsrII CGGWCCG 1 cut(s) 291
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 438, 495, 612
SatI GCNGC 4 cut(s) 183, 211, 252, 537
Sau3AI GATC 1 cut(s) 422
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 4 cut(s) 258, 348, 480, 554
SduI GDGCHC 2 cut(s) 300, 353
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 583
SinI GGWCC 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 4 cut(s) 434, 439, 490, 525
SsiI CCGC 7 cut(s) 97, 180, 208, 217, 231, 289, 536
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 4 cut(s) 256, 346, 478, 552
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 15, 23
TaqI TCGA 3 cut(s) 263, 407, 583
TasI AATT 4 cut(s) 434, 439, 490, 525
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 319, 384
Tru1I TTAA 3 cut(s) 438, 495, 612
Tru9I TTAA 3 cut(s) 438, 495, 612
TseI GCWGC 3 cut(s) 182, 210, 251
TspDTI ATGAA 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 2 cut(s) 434, 490
XceI RCATGY 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.