MD10G1058100.v1.1

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006023
Physical Location & Seq
Forward (+)
7862793 .. 7864776
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1058100.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGGCTTGCACATTCTGAGGTCATCTCCCCTTTCTTCCTCCAAGACCATAATCTTCAACCCCACCACCATCACACCCACTCTCTCCAACCTCCTCCGCCCTCCAAGCCCTAAACCCGCTTCTCTCTTCCGAACCCATTTCACAACTTTACCTCCAAAACCCATCGCAAGCGTTATACCCGCTGCTGGGTTTGCTGGGTCTTTGCACCGCAGCAAGCGGAGCTTTCGTGGCGGGGTAGTTGTAGCCATGGCTGCACCTGGGTCGGATCAGAAGTCTGAGGAGGAGTGGCGGACCGTGCTCTCTCCCGAGCAGTTTCGGATTCTGAGGCAGAAAGGCACAGAGTATCCGGGCACAGGAGAATATGACAAGTTCTTTGAGGAGGGAATCTATAACTGTGCAGGATGCGGGACTCCTCTCTACAGATCCACAACCAAATTTAATTCTGGTTGTGGCTGGCCAGCTTTTTATGAGGGTCTTCCTGGGGCCATAAATTTTAACCCAGACCCAGATGGAATGAGGACTGAAATTACATGTGCGGCTTGTGGTGGACACCTGGGTCATGTATTCAAAGGAGAAGGTTTTCCAACACCCACAGATGAACGCCATTGCGTCAATAGTATTTCACTCAAGTTTGCCCCTCCCACTTCTAATCCATCTCAGTGA

Protein Analysis

221

Amino Acids

23.77

Weight (kDa)

8.94

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SelR PF01641 100 - 213 2.5e-44 SelR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 555
AciI CCGC 9 cut(s) 97, 117, 180, 208, 217, 231, 289, 405, 536
AclWI GGATC 2 cut(s) 273, 417
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 2 cut(s) 434, 490
AfiI CCNNNNNNNGG 3 cut(s) 185, 186, 353
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 2 cut(s) 58, 568
AjnI CCWGG 3 cut(s) 256, 478, 552
AluBI AGCT 2 cut(s) 222, 461
AluI AGCT 2 cut(s) 222, 461
Alw21I GWGCWC 1 cut(s) 300
AlwI GGATC 2 cut(s) 273, 417
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 455, 483
ApeKI GCWGC 3 cut(s) 182, 210, 251
ApoI RAATTY 2 cut(s) 434, 490
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 1 cut(s) 579
AspS9I GGNCC 2 cut(s) 291, 483
AsuC2I CCSGG 1 cut(s) 348
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 169, 222, 238
BccI CCATC 4 cut(s) 77, 170, 503, 661
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 3 cut(s) 258, 480, 554
BciVI GTATCC 1 cut(s) 354
BcnI CCSGG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 4 cut(s) 183, 211, 252, 537
BlsI GCNGC 4 cut(s) 184, 212, 253, 538
Bme1390I CCNGG 4 cut(s) 258, 348, 480, 554
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 291, 483
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 4 cut(s) 258, 348, 480, 554
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 467
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 348
BsaJI CCNNGG 4 cut(s) 246, 257, 479, 553
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 211, 241
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 186, 353
Bse3DI GCAATG 1 cut(s) 604
BseBI CCWGG 3 cut(s) 258, 480, 554
BseDI CCNNGG 4 cut(s) 246, 257, 479, 553
BseGI GGATG 1 cut(s) 407
BseLI CCNNNNNNNGG 3 cut(s) 185, 186, 353
BseMI GCAATG 1 cut(s) 604
BseMII CTCAG 3 cut(s) 8, 267, 314
BseRI GAGGAG 5 cut(s) 83, 293, 296, 392, 402
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 3 cut(s) 169, 222, 238
BseYI CCCAGC 2 cut(s) 185, 194
BsgI GTGCAG 2 cut(s) 237, 417
BshFI GGCC 2 cut(s) 457, 485
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 185, 186, 353
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 2 cut(s) 457, 485
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 300, 353
Bsp143I GATC 2 cut(s) 265, 422
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 9 cut(s) 97, 117, 180, 208, 217, 231, 289, 405, 536
BspANI GGCC 2 cut(s) 457, 485
BspCNI CTCAG 3 cut(s) 9, 268, 315
BspLI GGNNCC 1 cut(s) 484
BspPI GGATC 2 cut(s) 273, 417
BsrDI GCAATG 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 246, 257, 479, 553
BssMI GATC 2 cut(s) 265, 422
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 258, 480, 554
Bst4CI ACNGT 2 cut(s) 295, 395
Bst6I CTCTTC 1 cut(s) 131
BstC8I GCNNGC 5 cut(s) 8, 169, 215, 455, 459
BstDEI CTNAG 4 cut(s) 17, 276, 323, 657
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 1 cut(s) 407
BstKTI GATC 2 cut(s) 268, 425
BstMBI GATC 2 cut(s) 265, 422
BstMWI GCNNNNNNNGC 6 cut(s) 105, 191, 219, 228, 251, 295
BstNI CCWGG 3 cut(s) 258, 480, 554
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 4 cut(s) 256, 346, 478, 552
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 169, 222, 238
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 2 cut(s) 457, 485
BtgI CCRYGG 1 cut(s) 246
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 1 cut(s) 407
Cac8I GCNNGC 5 cut(s) 8, 169, 215, 455, 459
Cfr13I GGNCC 2 cut(s) 291, 483
CpoI CGGWCCG 1 cut(s) 291
CseI GACGC 1 cut(s) 598
CspI CGGWCCG 1 cut(s) 291
CviAII CATG 3 cut(s) 247, 531, 560
DdeI CTNAG 4 cut(s) 17, 276, 323, 657
DpnI GATC 2 cut(s) 267, 424
DpnII GATC 2 cut(s) 265, 422
DrdI GACNNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 2 cut(s) 86, 304
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 305
EcoRII CCWGG 3 cut(s) 256, 478, 552
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 3 cut(s) 250, 534, 563
FaiI YATR 9 cut(s) 50, 176, 248, 363, 390, 468, 488, 532, 561
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 421
FatI CATG 3 cut(s) 246, 530, 559
FauI CCCGC 4 cut(s) 124, 187, 224, 398
Fnu4HI GCNGC 4 cut(s) 183, 211, 252, 537
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 4 cut(s) 183, 211, 252, 537
GluI GCNGC 4 cut(s) 183, 211, 252, 537
GsaI CCCAGC 2 cut(s) 189, 198
HaeIII GGCC 2 cut(s) 457, 485
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 598
Hin1II CATG 3 cut(s) 250, 534, 563
HinfI GANTC 3 cut(s) 319, 384, 409
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 7 cut(s) 18, 131, 265, 270, 277, 318, 324
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 1 cut(s) 569
HpyCH4III ACNGT 2 cut(s) 295, 395
HpyCH4V TGCA 4 cut(s) 10, 205, 254, 398
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 191, 219, 228, 251, 295
HpyF3I CTNAG 4 cut(s) 17, 276, 323, 657
Hsp92II CATG 3 cut(s) 250, 534, 563
Kzo9I GATC 2 cut(s) 265, 422
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 169, 222, 238
LweI GCATC 1 cut(s) 392
MalI GATC 2 cut(s) 267, 424
MboI GATC 2 cut(s) 265, 422
MboII GAAGA 4 cut(s) 27, 46, 118, 467
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 2 cut(s) 300, 353
MlsI TGGCCA 1 cut(s) 457
MluCI AATT 4 cut(s) 434, 439, 490, 525
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 3 cut(s) 111, 243, 608
Mox20I TGGCCA 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 1 cut(s) 457
MseI TTAA 2 cut(s) 438, 495
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 4 cut(s) 258, 348, 480, 554
MvaI CCWGG 3 cut(s) 258, 480, 554
MwoI GCNNNNNNNGC 6 cut(s) 105, 191, 219, 228, 251, 295
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 1 cut(s) 246
NdeII GATC 2 cut(s) 265, 422
NlaIII CATG 3 cut(s) 250, 534, 563
NlaIV GGNNCC 1 cut(s) 484
NspI RCATGY 1 cut(s) 534
PciI ACATGT 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 319, 384
PkrI GCNGC 4 cut(s) 184, 212, 253, 538
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PscI ACATGT 1 cut(s) 530
Psp6I CCWGG 3 cut(s) 256, 478, 552
PspFI CCCAGC 2 cut(s) 185, 194
PspGI CCWGG 3 cut(s) 256, 478, 552
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 2 cut(s) 291, 483
PsuI RGATCY 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 658
Rsr2I CGGWCCG 1 cut(s) 291
RsrII CGGWCCG 1 cut(s) 291
SaqAI TTAA 2 cut(s) 438, 495
SatI GCNGC 4 cut(s) 183, 211, 252, 537
Sau3AI GATC 2 cut(s) 265, 422
Sau96I GGNCC 2 cut(s) 291, 483
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 4 cut(s) 258, 348, 480, 554
SduI GDGCHC 2 cut(s) 300, 353
SetI ASST 8 cut(s) 23, 93, 154, 224, 259, 463, 555, 580
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 418
SinI GGWCC 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 4 cut(s) 434, 439, 490, 525
SsiI CCGC 9 cut(s) 97, 117, 180, 208, 217, 231, 289, 405, 536
StyD4I CCNGG 4 cut(s) 256, 346, 478, 552
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 295, 395
TasI AATT 4 cut(s) 434, 439, 490, 525
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 319, 384
Tru1I TTAA 2 cut(s) 438, 495
Tru9I TTAA 2 cut(s) 438, 495
TseI GCWGC 3 cut(s) 182, 210, 251
TspDTI ATGAA 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 2 cut(s) 434, 490
XceI RCATGY 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.