pycom10g04480

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006023
Physical Location & Seq
Forward (+)
4695438 .. 4697633
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04480.1

Sequence Viewer

Length: 666 bp
ATGGGCTTGCACATTCTGAGGTCATCTCCCCTTTCTTCCTCCAAGACCATAATCTTCAACCCCACCACCATCACCACACCCACTCTCTCCAACCTCCTCCGCCCTCCAAGCCCTAAACCCGCTTCTCTCTACCGAACCCGTTTCACAACTTTACCTCCAAAACCCATCGCAAGCGTTATACCGGCTGCTGGGTTTGCTGGGTCTTTGCACCGCAGCAAGCGGAGCTTTCGTGGCGGGGTAGTTGTAGCCATGGCTGCACCTGGGTCGGCTCAGAAGTCTGAGGAGGAGTGGCGGACCGTGCTCTCTCCCGAGCAGTTTCGGATTCTGAGGCAGAAAGGCACAGAGTATCCGGGCACAGGAGAATATGACAAGTTCTTTGAGGAGGGAATCTATAACTGTGCAGGATGCGGGACTCCTCTCTACAGATCCACAACCAAATTTAATTCTGGTTGTGGCTGGCCAGCTTTTTACGAGGGTCTTCCTGGGGCCATAAATTTTAATCCAGACCCAGATGGAATGAGGACTGAAATTACATGTGCGGCTTGTGGTGGACACCTGGGTCATGTATTCAAAGGAGAAGGTTTTCGGACACCCACAGATGAACGCCATTGCGTTAATAGTATTTCACTCAAATTTGCACCTCCCACTTCTAATCCATCTCAGTGA

Protein Analysis

222

Amino Acids

23.93

Weight (kDa)

9.34

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SelR PF01641 101 - 214 2.1e-44 SelR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 558
AciI CCGC 8 cut(s) 100, 120, 211, 220, 234, 292, 408, 539
AclWI GGATC 1 cut(s) 420
AcoI YGGCCR 1 cut(s) 458
AcsI RAATTY 3 cut(s) 437, 493, 632
AfiI CCNNNNNNNGG 2 cut(s) 188, 356
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 2 cut(s) 58, 571
AjnI CCWGG 3 cut(s) 259, 481, 555
AluBI AGCT 2 cut(s) 225, 464
AluI AGCT 2 cut(s) 225, 464
Alw21I GWGCWC 1 cut(s) 303
AlwI GGATC 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 2 cut(s) 458, 486
ApeKI GCWGC 3 cut(s) 185, 213, 254
ApoI RAATTY 3 cut(s) 437, 493, 632
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
Asp700I GAANNNNTTC 1 cut(s) 582
AspS9I GGNCC 2 cut(s) 294, 486
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 172, 225, 241
BccI CCATC 4 cut(s) 77, 173, 506, 664
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 3 cut(s) 261, 483, 557
BciVI GTATCC 1 cut(s) 357
BcnI CCSGG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 421
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 4 cut(s) 186, 214, 255, 540
BlsI GCNGC 4 cut(s) 187, 215, 256, 541
Bme1390I CCNGG 4 cut(s) 261, 351, 483, 557
Bme18I GGWCC 1 cut(s) 294
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 294, 486
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 4 cut(s) 261, 351, 483, 557
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuMI CCSGG 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 249, 260, 482, 556
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 214, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 356
Bse118I RCCGGY 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 3 cut(s) 261, 483, 557
BseDI CCNNGG 4 cut(s) 249, 260, 482, 556
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 2 cut(s) 188, 356
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 4 cut(s) 8, 270, 284, 317
BseRI GAGGAG 5 cut(s) 86, 296, 299, 395, 405
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 3 cut(s) 172, 225, 241
BseYI CCCAGC 2 cut(s) 188, 197
BsgI GTGCAG 2 cut(s) 240, 420
BshFI GGCC 2 cut(s) 460, 488
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 2 cut(s) 182, 350
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 188, 356
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 2 cut(s) 460, 488
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 303, 356
Bsp143I GATC 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 8 cut(s) 100, 120, 211, 220, 234, 292, 408, 539
BspANI GGCC 2 cut(s) 460, 488
BspCNI CTCAG 4 cut(s) 9, 271, 283, 318
BspLI GGNNCC 1 cut(s) 487
BspPI GGATC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 4 cut(s) 249, 260, 482, 556
BssMI GATC 1 cut(s) 425
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 3 cut(s) 261, 483, 557
Bst4CI ACNGT 2 cut(s) 298, 398
BstC8I GCNNGC 5 cut(s) 8, 172, 218, 458, 462
BstDEI CTNAG 5 cut(s) 17, 270, 279, 326, 660
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 410
BstKTI GATC 1 cut(s) 428
BstMBI GATC 1 cut(s) 425
BstMWI GCNNNNNNNGC 6 cut(s) 108, 194, 222, 231, 254, 298
BstNI CCWGG 3 cut(s) 261, 483, 557
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 4 cut(s) 259, 349, 481, 555
BstSFI CTRYAG 1 cut(s) 421
BstSLI GKGCMC 1 cut(s) 356
BstV1I GCAGC 3 cut(s) 172, 225, 241
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 2 cut(s) 460, 488
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 410
Cac8I GCNNGC 5 cut(s) 8, 172, 218, 458, 462
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 294, 486
CpoI CGGWCCG 1 cut(s) 294
CspI CGGWCCG 1 cut(s) 294
CviAII CATG 3 cut(s) 250, 534, 563
DdeI CTNAG 5 cut(s) 17, 270, 279, 326, 660
DpnI GATC 1 cut(s) 427
DpnII GATC 1 cut(s) 425
DrdI GACNNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 458
EciI GGCGGA 2 cut(s) 89, 307
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 308
EcoRII CCWGG 3 cut(s) 259, 481, 555
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 253, 537, 566
FaiI YATR 8 cut(s) 50, 179, 251, 366, 393, 491, 535, 564
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 1 cut(s) 424
FatI CATG 3 cut(s) 249, 533, 562
FauI CCCGC 3 cut(s) 127, 227, 401
Fnu4HI GCNGC 4 cut(s) 186, 214, 255, 540
FokI GGATG 1 cut(s) 417
Fsp4HI GCNGC 4 cut(s) 186, 214, 255, 540
GluI GCNGC 4 cut(s) 186, 214, 255, 540
GsaI CCCAGC 2 cut(s) 192, 201
HaeIII GGCC 2 cut(s) 460, 488
HapII CCGG 2 cut(s) 182, 350
Hin1II CATG 3 cut(s) 253, 537, 566
HinfI GANTC 3 cut(s) 322, 387, 412
HpaII CCGG 2 cut(s) 182, 350
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 551
Hpy188I TCNGA 6 cut(s) 18, 273, 280, 321, 327, 588
Hpy188III TCNNGA 2 cut(s) 308, 503
Hpy8I GTNNAC 1 cut(s) 551
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 2 cut(s) 298, 398
HpyCH4V TGCA 5 cut(s) 10, 208, 257, 401, 638
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 194, 222, 231, 254, 298
HpyF3I CTNAG 5 cut(s) 17, 270, 279, 326, 660
Hsp92II CATG 3 cut(s) 253, 537, 566
Kzo9I GATC 1 cut(s) 425
LmnI GCTCC 1 cut(s) 222
Lsp1109I GCAGC 3 cut(s) 172, 225, 241
LweI GCATC 1 cut(s) 395
MalI GATC 1 cut(s) 427
MboI GATC 1 cut(s) 425
MboII GAAGA 3 cut(s) 27, 46, 470
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 2 cut(s) 303, 356
MlsI TGGCCA 1 cut(s) 460
MluCI AATT 5 cut(s) 437, 442, 493, 528, 632
MluNI TGGCCA 1 cut(s) 460
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 114
Mox20I TGGCCA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 460
MseI TTAA 3 cut(s) 441, 498, 615
MslI CAYNNNNRTG 1 cut(s) 661
Msp20I TGGCCA 1 cut(s) 460
MspI CCGG 2 cut(s) 182, 350
MspR9I CCNGG 4 cut(s) 261, 351, 483, 557
MvaI CCWGG 3 cut(s) 261, 483, 557
MwoI GCNNNNNNNGC 6 cut(s) 108, 194, 222, 231, 254, 298
NciI CCSGG 1 cut(s) 351
NcoI CCATGG 1 cut(s) 249
NdeII GATC 1 cut(s) 425
NlaIII CATG 3 cut(s) 253, 537, 566
NlaIV GGNNCC 1 cut(s) 487
NspI RCATGY 1 cut(s) 537
PciI ACATGT 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 2 cut(s) 322, 387
PkrI GCNGC 4 cut(s) 187, 215, 256, 541
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PscI ACATGT 1 cut(s) 533
Psp6I CCWGG 3 cut(s) 259, 481, 555
PspFI CCCAGC 2 cut(s) 188, 197
PspGI CCWGG 3 cut(s) 259, 481, 555
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 2 cut(s) 294, 486
PsuI RGATCY 1 cut(s) 425
RseI CAYNNNNRTG 1 cut(s) 661
Rsr2I CGGWCCG 1 cut(s) 294
RsrII CGGWCCG 1 cut(s) 294
SaqAI TTAA 3 cut(s) 441, 498, 615
SatI GCNGC 4 cut(s) 186, 214, 255, 540
Sau3AI GATC 1 cut(s) 425
Sau96I GGNCC 2 cut(s) 294, 486
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 4 cut(s) 261, 351, 483, 557
SduI GDGCHC 2 cut(s) 303, 356
SetI ASST 9 cut(s) 23, 96, 157, 227, 262, 466, 558, 583, 643
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 421
SinI GGWCC 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 661
Sse9I AATT 5 cut(s) 437, 442, 493, 528, 632
SsiI CCGC 8 cut(s) 100, 120, 211, 220, 234, 292, 408, 539
StyD4I CCNGG 4 cut(s) 259, 349, 481, 555
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 298, 398
TasI AATT 5 cut(s) 437, 442, 493, 528, 632
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 2 cut(s) 322, 387
Tru1I TTAA 3 cut(s) 441, 498, 615
Tru9I TTAA 3 cut(s) 441, 498, 615
TseI GCWGC 3 cut(s) 185, 213, 254
TspDTI ATGAA 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 294
XapI RAATTY 3 cut(s) 437, 493, 632
XceI RCATGY 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.