Rh6DG157100

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006023
Physical Location & Seq
Reverse (-)
23557308 .. 23560567
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG157100.1

Sequence Viewer

Length: 666 bp
ATGGGCCTGCACATTCTGAGGTCTTCTTCACCCCTTTCTTCTTCTTCCAAGACCATAATCTTCAACCCCACAACCAAACCTCCTCTAACAAACCTCCTCCTCCCCCGACCTTCAAACCCTACACCCACTACTTCTCTCTTCAAAACCCATTTCACAACTCTCTCACCAAAACCCATCACACCCGCCATTGGGTTTGGTGGGTCTTTCCAAAACACTAGGCGTAGCTTCCGTGGTGGAGTTGTTGTAGCCATGGCTGCACCTGGCCCGGTTCAGAAATCAGAGGAGGAGTGGAGAGCTGTCCTCTCTCCAGAGCAGTTTCGGATTCTGAGGCAAAAAGGCACCGAGTATCCTGGCACCGGAGTATATGACAAGTTCTTTGAGGAGGGAGTCTATACCTGTGCAGGATGTGGGACTCCTCTCTACAAGTCAACAACCAAATTTAACTCTGGTTGTGGTTGGCCAGCTTTCTACGAGGGTCTTCCTGGGGCCATAAATTCTGTTCCGGACCGCGATGGGATGAGGGTTGAAATTACATGTGCGGCTTGTGGTGGACACCTGGGTCATGTATTCAAAGGTGAAGGTTTCCGGACACCCACAGATGAACGCCATTGTGTGAACAGTATTTCACTCAAGTTTAGTCCTGGCAATTCTAATCCTTCTGAGTGA

Protein Analysis

221

Amino Acids

23.86

Weight (kDa)

9.22

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SelR PF01641 101 - 214 4.4e-44 SelR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 558
AccB1I GGYRCC 2 cut(s) 338, 353
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 2 cut(s) 502, 585
AciI CCGC 3 cut(s) 183, 508, 539
AcoI YGGCCR 1 cut(s) 458
AcsI RAATTY 2 cut(s) 437, 493
AfiI CCNNNNNNNGG 3 cut(s) 188, 189, 356
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 5 cut(s) 64, 114, 142, 527, 571
AjnI CCWGG 5 cut(s) 259, 349, 481, 555, 640
AluBI AGCT 3 cut(s) 225, 296, 464
AluI AGCT 3 cut(s) 225, 296, 464
Aor13HI TCCGGA 2 cut(s) 502, 585
AoxI GGCC 4 cut(s) 4, 262, 458, 486
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 2 cut(s) 437, 493
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AspS9I GGNCC 4 cut(s) 4, 263, 486, 505
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 21, 156, 587
AvaII GGWCC 1 cut(s) 505
BalI TGGCCA 1 cut(s) 460
BanI GGYRCC 2 cut(s) 338, 353
BbsI GAAGAC 2 cut(s) 15, 470
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 182, 506
BciT130I CCWGG 5 cut(s) 261, 351, 483, 557, 642
BciVI GTATCC 1 cut(s) 357
BcnI CCSGG 1 cut(s) 266
BfaI CTAG 1 cut(s) 216
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 2 cut(s) 255, 540
BlsI GCNGC 2 cut(s) 256, 541
Bme1390I CCNGG 6 cut(s) 261, 266, 351, 483, 557, 642
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 4 cut(s) 4, 263, 486, 505
BmiI GGNNCC 3 cut(s) 340, 355, 487
BmrFI CCNGG 6 cut(s) 261, 266, 351, 483, 557, 642
BpiI GAAGAC 2 cut(s) 15, 470
BplI GAGNNNNNCTC 2 cut(s) 285, 317
BpmI CTGGAG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 229, 249, 482, 556
BsaWI WCCGGW 3 cut(s) 356, 502, 585
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 228, 258
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 189, 356
BseAI TCCGGA 2 cut(s) 502, 585
BseBI CCWGG 5 cut(s) 261, 351, 483, 557, 642
BseDI CCNNGG 4 cut(s) 229, 249, 482, 556
BseGI GGATG 2 cut(s) 410, 522
BseLI CCNNNNNNNGG 3 cut(s) 188, 189, 356
BseMII CTCAG 3 cut(s) 8, 317, 651
BseRI GAGGAG 7 cut(s) 72, 86, 89, 296, 299, 395, 405
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 2 cut(s) 240, 420
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 4 cut(s) 6, 264, 460, 488
BshNI GGYRCC 2 cut(s) 338, 353
BsiSI CCGG 4 cut(s) 266, 357, 503, 586
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 3 cut(s) 188, 189, 356
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 4 cut(s) 6, 264, 460, 488
Bsp13I TCCGGA 2 cut(s) 502, 585
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 183, 508, 539
BspANI GGCC 4 cut(s) 6, 264, 460, 488
BspCNI CTCAG 3 cut(s) 9, 318, 652
BspEI TCCGGA 2 cut(s) 502, 585
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 3 cut(s) 340, 355, 487
BspT107I GGYRCC 2 cut(s) 338, 353
BssECI CCNNGG 4 cut(s) 229, 249, 482, 556
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 5 cut(s) 261, 351, 483, 557, 642
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 8, 462
BstDEI CTNAG 3 cut(s) 17, 326, 660
BstDSI CCRYGG 2 cut(s) 229, 249
BstF5I GGATG 2 cut(s) 410, 522
BstFNI CGCG 1 cut(s) 510
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 5 cut(s) 261, 351, 483, 557, 642
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 6 cut(s) 259, 264, 349, 481, 555, 640
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 2 cut(s) 15, 470
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 4 cut(s) 6, 264, 460, 488
BtgI CCRYGG 2 cut(s) 229, 249
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 2 cut(s) 410, 522
Cac8I GCNNGC 2 cut(s) 8, 462
Cfr13I GGNCC 4 cut(s) 4, 263, 486, 505
CpoI CGGWCCG 1 cut(s) 505
CspI CGGWCCG 1 cut(s) 505
CviAII CATG 3 cut(s) 250, 534, 563
DdeI CTNAG 3 cut(s) 17, 326, 660
DrdI GACNNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 458
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 505
EcoRII CCWGG 5 cut(s) 259, 349, 481, 555, 640
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 253, 537, 566
FaiI YATR 8 cut(s) 56, 251, 364, 366, 393, 491, 535, 564
FaqI GGGAC 1 cut(s) 424
FatI CATG 3 cut(s) 249, 533, 562
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 255, 540
FokI GGATG 2 cut(s) 417, 529
Fsp4HI GCNGC 2 cut(s) 255, 540
FspBI CTAG 1 cut(s) 216
GluI GCNGC 2 cut(s) 255, 540
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 4 cut(s) 6, 264, 460, 488
HapII CCGG 4 cut(s) 266, 357, 503, 586
Hin1II CATG 3 cut(s) 253, 537, 566
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HinfI GANTC 3 cut(s) 322, 387, 412
HpaII CCGG 4 cut(s) 266, 357, 503, 586
HphI GGTGA 3 cut(s) 21, 156, 587
Hpy166II GTNNAC 3 cut(s) 429, 551, 616
Hpy188I TCNGA 6 cut(s) 18, 273, 280, 321, 327, 661
Hpy188III TCNNGA 3 cut(s) 308, 503, 586
Hpy8I GTNNAC 3 cut(s) 429, 551, 616
HpyAV CCTTC 3 cut(s) 120, 572, 666
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4V TGCA 3 cut(s) 10, 257, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 17, 326, 660
Hsp92II CATG 3 cut(s) 253, 537, 566
Kpn2I TCCGGA 2 cut(s) 502, 585
Lsp1109I GCAGC 1 cut(s) 241
MaeI CTAG 1 cut(s) 216
MboII GAAGA 8 cut(s) 15, 18, 30, 33, 36, 52, 130, 470
MlsI TGGCCA 1 cut(s) 460
MluCI AATT 4 cut(s) 437, 493, 528, 646
MluNI TGGCCA 1 cut(s) 460
MlyI GAGTC 2 cut(s) 396, 406
Mox20I TGGCCA 1 cut(s) 460
MroI TCCGGA 2 cut(s) 502, 585
MscI TGGCCA 1 cut(s) 460
MseI TTAA 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 460
MspI CCGG 4 cut(s) 266, 357, 503, 586
MspR9I CCNGG 6 cut(s) 261, 266, 351, 483, 557, 642
MvaI CCWGG 5 cut(s) 261, 351, 483, 557, 642
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 249
NlaIII CATG 3 cut(s) 253, 537, 566
NlaIV GGNNCC 3 cut(s) 340, 355, 487
NspI RCATGY 1 cut(s) 537
PciI ACATGT 1 cut(s) 533
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 2 cut(s) 256, 541
PleI GAGTC 2 cut(s) 395, 406
PpsI GAGTC 2 cut(s) 395, 406
PscI ACATGT 1 cut(s) 533
Psp6I CCWGG 5 cut(s) 259, 349, 481, 555, 640
PspGI CCWGG 5 cut(s) 259, 349, 481, 555, 640
PspN4I GGNNCC 3 cut(s) 340, 355, 487
PspPI GGNCC 4 cut(s) 4, 263, 486, 505
Rsr2I CGGWCCG 1 cut(s) 505
RsrII CGGWCCG 1 cut(s) 505
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 255, 540
Sau96I GGNCC 4 cut(s) 4, 263, 486, 505
SchI GAGTC 2 cut(s) 396, 406
ScrFI CCNGG 6 cut(s) 261, 266, 351, 483, 557, 642
SinI GGWCC 1 cut(s) 505
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 4 cut(s) 437, 493, 528, 646
SsiI CCGC 3 cut(s) 183, 508, 539
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 6 cut(s) 259, 264, 349, 481, 555, 640
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 620
TasI AATT 4 cut(s) 437, 493, 528, 646
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 615
TspGWI ACGGA 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 2 cut(s) 437, 493
XceI RCATGY 1 cut(s) 537
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.