Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008976

Physical Location & Seq

Genomic Coordinates Forward (+)

20859919 .. 20863203

Gene Structure 3285 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1100600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGATTTTGCGAGATATGCAAGCCAAGTCACTGCAATGGGCGCCGTCGCCGGGACTCCGGGTCGGGGTCGAAACGTCTTCTTTGTCGCAGCTGGCGGTCCTGAACTTGCCCCGTGGTGCTTCTGTGAATGGCTTGAAAGCCAGGAGTTTGTTTGTAGTAAAGGCCATGGAGGTGGAGGGTTCCAGAACGGCGAGTCTGAAAGGCCACGCTGGGAGTTCAGATAAAAATGGGAGCTTGTTGTCTTCGGTTGGCAATTCAACCAATATTAAATGGCATGAGTGTTCATTGAACAAAACTGACAGGCAAAAGTTGCTTAAACAAAAGGGGTGTGTCATTTGGATCACCGGTCTCAGCGGTTCAGGGAAGAGCACGGTGGCTTGTGCATTGGGTGGGAGTTTGTATCACAGGGGAAAACTATCGTACATTCTTGATGGGGACAATGTCAGGCATGGCCTGAATTGCGACCTTAGTTTTAAAGCAGAAGATCGTGCAGAGAACATTCGGAGGATTGGGGAGGTGGCAAAATTGTTTGCTGATGCTGGAGTTATTTGTATTGCTAGCGTGGTTTCTCCTTATAGAAGGGATCGTGATGCCTGCCGTGCAATGCTACCTGCTGGAGACTTCATTGAGGTGTTCATGGACGTTCCACTCCAAGTTTGTGAGGCTAGAGACCCGAAAGGCCTATATAAGCTTGCACGTGCAGGGAAGATCGAAGGTTTTACTGGGATTGATGATCCATATGAGCAACCGTTAGATTGTGAGATAGTATTATCACATAAAGGAGGAGCATGTCCTTCCCCATGTGAGATGGCCGAAGAAGTAGTTTCTTACTTGGAAGATCAGGGGTTCCTCCAGGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004020 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00230 ko00920 ko01100 ko01120 map00230 map00920 map01100 map01120

Pfam Domains

Protein Families

APS_kinase

Protein Analysis

287

Amino Acids

30.82

Weight (kDa)

8.0

Isoelectric Point (pI)

43.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APS_kinase	PF01583	108 - 257	6.7e-68	Adenylylsulphate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G14750
fragaria_vesca	FvH4_2g04460
malus_domestica	MD05G1100600.v1.1 MD10G1105800.v1.1
prunus_persica	Prupe.8G145000_v2.0.a1 Prupe.8G145000_v2.0.a1
pyrus_communis	pycom05g09920 pycom10g09120
rosa_chinensis	RchiOBHm_Chr6g0253831
rosa_laevigata	RLG00000014962
rosa_multiflora	Rmu_co7978700.1_g000001
rosa_roxburghii	Rroxscaffold_7G00211480
rosa_rugosa	Rorug05G0548000 Rorug05G0548100 Rorug07G0194700
rosa_samantha	Rh4AG288600 Rh5AG196500 Rh6AG063600 Rh6BG057100 Rh6CG057100 Rh6DG054300
rosa_wichuraiana	Rw6G005640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	619
AccB1I	GGYRCC	1 cut(s)	40
AciI	CCGC	2 cut(s)	95, 354
AclWI	GGATC	3 cut(s)	347, 591, 728
AcoI	YGGCCR	1 cut(s)	810
AcvI	CACGTG	1 cut(s)	698
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	1 cut(s)	422
AfiI	CCNNNNNNNGG	2 cut(s)	50, 64
AgeI	ACCGGT	1 cut(s)	344
AgsI	TTSAA	3 cut(s)	136, 258, 289
AhdI	GACNNNNNGTC	1 cut(s)	59
AjnI	CCWGG	2 cut(s)	140, 852
AluBI	AGCT	3 cut(s)	91, 234, 691
AluI	AGCT	3 cut(s)	91, 234, 691
Alw21I	GWGCWC	1 cut(s)	371
Alw26I	GTCTC	3 cut(s)	353, 612, 663
AlwI	GGATC	3 cut(s)	347, 591, 728
AoxI	GGCC	5 cut(s)	162, 202, 451, 679, 810
ApeKI	GCWGC	1 cut(s)	88
AsiGI	ACCGGT	1 cut(s)	344
AspLEI	GCGC	1 cut(s)	43
AspS9I	GGNCC	1 cut(s)	97
AsuC2I	CCSGG	2 cut(s)	51, 59
AsuHPI	GGTGA	1 cut(s)	334
AsuNHI	GCTAGC	1 cut(s)	557
AvaII	GGWCC	1 cut(s)	97
BanI	GGYRCC	1 cut(s)	40
BbrPI	CACGTG	1 cut(s)	698
BbsI	GAAGAC	2 cut(s)	69, 234
Bbv12I	GWGCWC	1 cut(s)	371
BbvI	GCAGC	1 cut(s)	100
BccI	CCATC	2 cut(s)	425, 802
BceAI	ACGGC	3 cut(s)	28, 204, 582
BciT130I	CCWGG	2 cut(s)	142, 854
BcnI	CCSGG	2 cut(s)	51, 59
BcoDI	GTCTC	3 cut(s)	353, 612, 663
BfaI	CTAG	2 cut(s)	558, 666
BfoI	RGCGCY	1 cut(s)	44
BfuAI	ACCTGC	1 cut(s)	619
BisI	GCNGC	1 cut(s)	89
BlsI	GCNGC	1 cut(s)	90
Bme1390I	CCNGG	4 cut(s)	51, 59, 142, 854
Bme18I	GGWCC	1 cut(s)	97
BmeRI	GACNNNNNGTC	1 cut(s)	59
BmgT120I	GGNCC	1 cut(s)	97
BmiI	GGNNCC	3 cut(s)	42, 181, 848
BmrFI	CCNGG	4 cut(s)	51, 59, 142, 854
BmrI	ACTGGG	1 cut(s)	732
BmsI	GCATC	2 cut(s)	526, 580
BmtI	GCTAGC	1 cut(s)	561
BmuI	ACTGGG	1 cut(s)	732
BpiI	GAAGAC	2 cut(s)	69, 234
BpmI	CTGGAG	3 cut(s)	561, 636, 836
BpuMI	CCSGG	2 cut(s)	51, 59
BsaAI	YACGTR	1 cut(s)	698
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	2 cut(s)	353, 663
BsaJI	CCNNGG	2 cut(s)	112, 165
BsaWI	WCCGGW	1 cut(s)	344
Bsc4I	CCNNNNNNNGG	2 cut(s)	50, 64
Bse118I	RCCGGY	1 cut(s)	344
Bse1I	ACTGG	1 cut(s)	727
Bse3DI	GCAATG	2 cut(s)	41, 609
BseBI	CCWGG	2 cut(s)	142, 854
BseDI	CCNNGG	2 cut(s)	112, 165
BseLI	CCNNNNNNNGG	2 cut(s)	50, 64
BseMI	GCAATG	2 cut(s)	41, 609
BseMII	CTCAG	1 cut(s)	364
BseNI	ACTGG	1 cut(s)	727
BseRI	GAGGAG	1 cut(s)	798
BseXI	GCAGC	1 cut(s)	100
BseYI	CCCAGC	1 cut(s)	209
BsgI	GTGCAG	2 cut(s)	510, 720
BshFI	GGCC	5 cut(s)	164, 204, 453, 681, 812
BshNI	GGYRCC	1 cut(s)	40
BshTI	ACCGGT	1 cut(s)	344
BsiHKAI	GWGCWC	1 cut(s)	371
BsiSI	CCGG	3 cut(s)	50, 58, 345
BslFI	GGGAC	2 cut(s)	66, 449
BslI	CCNNNNNNNGG	2 cut(s)	50, 64
BsmAI	GTCTC	3 cut(s)	353, 612, 663
BsmFI	GGGAC	2 cut(s)	66, 449
BsnI	GGCC	5 cut(s)	164, 204, 453, 681, 812
Bso31I	GGTCTC	2 cut(s)	353, 663
Bsp1286I	GDGCHC	1 cut(s)	371
Bsp143I	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
Bsp19I	CCATGG	1 cut(s)	165
BspACI	CCGC	2 cut(s)	95, 354
BspANI	GGCC	5 cut(s)	164, 204, 453, 681, 812
BspCNI	CTCAG	1 cut(s)	363
BspLI	GGNNCC	3 cut(s)	42, 181, 848
BspMI	ACCTGC	1 cut(s)	619
BspOI	GCTAGC	1 cut(s)	561
BspPI	GGATC	3 cut(s)	347, 591, 728
BspQI	GCTCTTC	1 cut(s)	359
BspT107I	GGYRCC	1 cut(s)	40
BspTNI	GGTCTC	2 cut(s)	353, 663
BsrDI	GCAATG	2 cut(s)	41, 609
BsrFI	RCCGGY	1 cut(s)	344
BsrI	ACTGG	1 cut(s)	727
BssAI	RCCGGY	1 cut(s)	344
BssECI	CCNNGG	2 cut(s)	112, 165
BssMI	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
BssNI	GRCGYC	1 cut(s)	41
BssT1I	CCWWGG	1 cut(s)	165
Bst2UI	CCWGG	2 cut(s)	142, 854
Bst4CI	ACNGT	2 cut(s)	373, 750
Bst6I	CTCTTC	1 cut(s)	359
BstACI	GRCGYC	1 cut(s)	41
BstAPI	GCANNNNNTGC	1 cut(s)	310
BstBAI	YACGTR	1 cut(s)	698
BstC8I	GCNNGC	5 cut(s)	21, 93, 559, 595, 693
BstDEI	CTNAG	2 cut(s)	350, 467
BstDSI	CCRYGG	2 cut(s)	112, 165
BstH2I	RGCGCY	1 cut(s)	44
BstHHI	GCGC	1 cut(s)	43
BstKTI	GATC	6 cut(s)	342, 487, 586, 711, 736, 841
BstMAI	GTCTC	3 cut(s)	353, 612, 663
BstMBI	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
BstMWI	GCNNNNNNNGC	5 cut(s)	16, 40, 310, 459, 599
BstNI	CCWGG	2 cut(s)	142, 854
BstNSI	RCATGY	1 cut(s)	792
BstSCI	CCNGG	4 cut(s)	49, 57, 140, 852
BstV1I	GCAGC	1 cut(s)	100
BstV2I	GAAGAC	2 cut(s)	69, 234
BstXI	CCANNNNNNTGG	1 cut(s)	172
BsuRI	GGCC	5 cut(s)	164, 204, 453, 681, 812
BtgI	CCRYGG	2 cut(s)	112, 165
BtsI	GCAGTG	1 cut(s)	29
BtsIMutI	CAGTG	1 cut(s)	29
BveI	ACCTGC	1 cut(s)	619
Cac8I	GCNNGC	5 cut(s)	21, 93, 559, 595, 693
CfoI	GCGC	1 cut(s)	43
Cfr10I	RCCGGY	1 cut(s)	344
Cfr13I	GGNCC	1 cut(s)	97
Csp6I	GTAC	1 cut(s)	421
CspAI	ACCGGT	1 cut(s)	344
CviAII	CATG	6 cut(s)	166, 275, 449, 637, 789, 801
CviQI	GTAC	1 cut(s)	421
DdeI	CTNAG	2 cut(s)	350, 467
DinI	GGCGCC	1 cut(s)	42
DpnI	GATC	6 cut(s)	341, 486, 585, 710, 735, 840
DpnII	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
DraI	TTTAAA	1 cut(s)	475
DriI	GACNNNNNGTC	1 cut(s)	59
EaeI	YGGCCR	1 cut(s)	810
Eam1104I	CTCTTC	1 cut(s)	359
Eam1105I	GACNNNNNGTC	1 cut(s)	59
EarI	CTCTTC	1 cut(s)	359
Eco130I	CCWWGG	1 cut(s)	165
Eco147I	AGGCCT	1 cut(s)	681
Eco31I	GGTCTC	2 cut(s)	353, 663
Eco47I	GGWCC	1 cut(s)	97
Eco72I	CACGTG	1 cut(s)	698
EcoRII	CCWGG	2 cut(s)	140, 852
EcoT14I	CCWWGG	1 cut(s)	165
EgeI	GGCGCC	1 cut(s)	42
EheI	GGCGCC	1 cut(s)	42
ErhI	CCWWGG	1 cut(s)	165
FaeI	CATG	6 cut(s)	169, 278, 452, 640, 792, 804
FaqI	GGGAC	2 cut(s)	66, 449
FatI	CATG	6 cut(s)	165, 274, 448, 636, 788, 800
FauNDI	CATATG	1 cut(s)	739
Fnu4HI	GCNGC	1 cut(s)	89
Fsp4HI	GCNGC	1 cut(s)	89
FspBI	CTAG	2 cut(s)	558, 666
GlaI	GCGC	1 cut(s)	42
GluI	GCNGC	1 cut(s)	89
GsaI	CCCAGC	1 cut(s)	213
GsuI	CTGGAG	3 cut(s)	561, 636, 836
HaeII	RGCGCY	1 cut(s)	44
HaeIII	GGCC	5 cut(s)	164, 204, 453, 681, 812
HapII	CCGG	3 cut(s)	50, 58, 345
HhaI	GCGC	1 cut(s)	43
Hin1I	GRCGYC	1 cut(s)	41
Hin1II	CATG	6 cut(s)	169, 278, 452, 640, 792, 804
Hin6I	GCGC	1 cut(s)	41
HinP1I	GCGC	1 cut(s)	41
HindIII	AAGCTT	1 cut(s)	689
HinfI	GANTC	2 cut(s)	54, 193
HpaII	CCGG	3 cut(s)	50, 58, 345
HphI	GGTGA	1 cut(s)	334
Hpy188I	TCNGA	3 cut(s)	198, 220, 504
Hpy188III	TCNNGA	4 cut(s)	100, 183, 428, 587
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	3 cut(s)	573, 707, 804
HpyCH4III	ACNGT	2 cut(s)	373, 750
HpyCH4IV	ACGT	3 cut(s)	74, 642, 697
HpyCH4V	TGCA	7 cut(s)	19, 34, 383, 491, 602, 695, 701
HpyF10VI	GCNNNNNNNGC	5 cut(s)	16, 40, 310, 459, 599
HpyF3I	CTNAG	2 cut(s)	350, 467
HpySE526I	ACGT	3 cut(s)	74, 642, 697
Hsp92I	GRCGYC	1 cut(s)	41
Hsp92II	CATG	6 cut(s)	169, 278, 452, 640, 792, 804
HspAI	GCGC	1 cut(s)	41
KasI	GGCGCC	1 cut(s)	40
Kzo9I	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
LguI	GCTCTTC	1 cut(s)	359
LmnI	GCTCC	2 cut(s)	231, 785
Lsp1109I	GCAGC	1 cut(s)	100
LweI	GCATC	2 cut(s)	526, 580
MaeI	CTAG	2 cut(s)	558, 666
MaeII	ACGT	3 cut(s)	74, 642, 697
MaeIII	GTNAC	1 cut(s)	27
MalI	GATC	6 cut(s)	341, 486, 585, 710, 735, 840
MboI	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
MboII	GAAGA	7 cut(s)	69, 234, 376, 494, 718, 827, 848
MhlI	GDGCHC	1 cut(s)	371
MluCI	AATT	3 cut(s)	253, 457, 524
Mly113I	GGCGCC	1 cut(s)	41
MlyI	GAGTC	2 cut(s)	48, 202
MnlI	CCTC	8 cut(s)	163, 169, 498, 508, 622, 655, 776, 860
MseI	TTAA	3 cut(s)	267, 315, 474
MslI	CAYNNNNRTG	3 cut(s)	34, 170, 629
MspA1I	CMGCKG	2 cut(s)	91, 354
MspI	CCGG	3 cut(s)	50, 58, 345
MspR9I	CCNGG	4 cut(s)	51, 59, 142, 854
MvaI	CCWGG	2 cut(s)	142, 854
MwoI	GCNNNNNNNGC	5 cut(s)	16, 40, 310, 459, 599
NarI	GGCGCC	1 cut(s)	41
NciI	CCSGG	2 cut(s)	51, 59
NcoI	CCATGG	1 cut(s)	165
NdeI	CATATG	1 cut(s)	739
NdeII	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
NheI	GCTAGC	1 cut(s)	557
NlaIII	CATG	6 cut(s)	169, 278, 452, 640, 792, 804
NlaIV	GGNNCC	3 cut(s)	42, 181, 848
NmuCI	GTSAC	1 cut(s)	27
NspI	RCATGY	1 cut(s)	792
PceI	AGGCCT	1 cut(s)	681
PciSI	GCTCTTC	1 cut(s)	359
PflFI	GACNNNGTC	1 cut(s)	440
PinAI	ACCGGT	1 cut(s)	344
PkrI	GCNGC	1 cut(s)	90
PleI	GAGTC	2 cut(s)	48, 201
PluTI	GGCGCC	1 cut(s)	44
PmaCI	CACGTG	1 cut(s)	698
PmlI	CACGTG	1 cut(s)	698
PpsI	GAGTC	2 cut(s)	48, 201
Ppu21I	YACGTR	1 cut(s)	698
Psp6I	CCWGG	2 cut(s)	140, 852
PspCI	CACGTG	1 cut(s)	698
PspFI	CCCAGC	1 cut(s)	209
PspGI	CCWGG	2 cut(s)	140, 852
PspN4I	GGNNCC	3 cut(s)	42, 181, 848
PspPI	GGNCC	1 cut(s)	97
PsyI	GACNNNGTC	1 cut(s)	440
PvuII	CAGCTG	1 cut(s)	91
RsaI	GTAC	1 cut(s)	422
RsaNI	GTAC	1 cut(s)	421
RseI	CAYNNNNRTG	3 cut(s)	34, 170, 629
SapI	GCTCTTC	1 cut(s)	359
SaqAI	TTAA	3 cut(s)	267, 315, 474
SatI	GCNGC	1 cut(s)	89
Sau3AI	GATC	6 cut(s)	339, 484, 583, 708, 733, 838
Sau96I	GGNCC	1 cut(s)	97
SchI	GAGTC	2 cut(s)	48, 202
ScrFI	CCNGG	4 cut(s)	51, 59, 142, 854
SduI	GDGCHC	1 cut(s)	371
SfaNI	GCATC	2 cut(s)	526, 580
SfoI	GGCGCC	1 cut(s)	42
SinI	GGWCC	1 cut(s)	97
SmiMI	CAYNNNNRTG	3 cut(s)	34, 170, 629
Sse9I	AATT	3 cut(s)	253, 457, 524
SseBI	AGGCCT	1 cut(s)	681
SsiI	CCGC	2 cut(s)	95, 354
SspDI	GGCGCC	1 cut(s)	40
SspI	AATATT	1 cut(s)	265
SspMI	CTAG	2 cut(s)	558, 666
StuI	AGGCCT	1 cut(s)	681
StyD4I	CCNGG	4 cut(s)	49, 57, 140, 852
StyI	CCWWGG	1 cut(s)	165
TaaI	ACNGT	2 cut(s)	373, 750
TaiI	ACGT	3 cut(s)	77, 645, 700
TaqI	TCGA	2 cut(s)	69, 711
TasI	AATT	3 cut(s)	253, 457, 524
Tru1I	TTAA	3 cut(s)	267, 315, 474
Tru9I	TTAA	3 cut(s)	267, 315, 474
TscAI	CASTG	1 cut(s)	36
TseFI	GTSAC	1 cut(s)	27
TseI	GCWGC	1 cut(s)	88
Tsp45I	GTSAC	1 cut(s)	27
TspDTI	ATGAA	3 cut(s)	273, 613, 625
TspRI	CASTG	1 cut(s)	36
Tth111I	GACNNNGTC	1 cut(s)	440
VpaK11BI	GGWCC	1 cut(s)	97
XceI	RCATGY	1 cut(s)	792
XspI	CTAG	2 cut(s)	558, 666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.