Rh6BG057100

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008976
Physical Location & Seq
Forward (+)
9103865 .. 9108726
4862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG057100.1

Sequence Viewer

Length: 639 bp
ATGAATGCCAGCCGGTTGCAATGGGCATCTTCCTCGCCTGCGCTGAGCACAGGGCTCGAAACGTCGTCGTCTTGTTCGAAGCTGGGTTTTGTGAACTTTCGCGGCGGCGGCGGCGGTTGTGGAGATAGCTTGAAGACGAAGAAGGGTTTGTTTGTGATAAAGGCGATGGATGTGGAGGGTTCGAGAACCGTGAATTTGAATGGGCACGCTGGGGATTCAGATAAAAATGGGAGCTTGTTGTCTTCGATTGGAAATTCGACCAACATTCAGTGGCACCAGTGCTCGGTGAAGAAAGAAGATAGGGAAAATATGCTGAAACAAAAAGGGTGTGTTATATGGATCACTGGGCTTAGTGGATCAGGGAAGAGCACTGTGGCTTGTGCTTTGAGTGGGATCTTGTACCGCAGGGGAAAGCTGGCTTATGTTCTAGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGACCGTGCAGAGAACATAAGGAGGATTGGGGAGGTGGCAAAGCTGTTTGCCGATGCTGGAGTCATTTGTATTGCTAGCTTGATTTCTCCGTATAGAAGGGATCGTGATGCCTGTCGTGCATTATTGCCTGCTGGAGAGTTTATTGAGGTTGGTTAG

Protein Analysis

212

Amino Acids

22.6

Weight (kDa)

9.24

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 108 - 211 5.3e-44 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 273
AccII CGCG 1 cut(s) 102
AciI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
AclWI GGATC 4 cut(s) 347, 364, 401, 591
AcsI RAATTY 2 cut(s) 193, 253
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 133, 199, 457
AluBI AGCT 6 cut(s) 82, 129, 234, 415, 526, 561
AluI AGCT 6 cut(s) 82, 129, 234, 415, 526, 561
Alw21I GWGCWC 3 cut(s) 50, 284, 371
AlwI GGATC 4 cut(s) 347, 364, 401, 591
ApoI RAATTY 2 cut(s) 193, 253
AspLEI GCGC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 298
AsuII TTCGAA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 57
BbsI GAAGAC 3 cut(s) 140, 234, 489
Bbv12I GWGCWC 3 cut(s) 50, 284, 371
BccI CCATC 2 cut(s) 160, 425
BcgI CGANNNNNNTGC 4 cut(s) 15, 37, 49, 71
BfaI CTAG 2 cut(s) 428, 558
BisI GCNGC 4 cut(s) 103, 106, 109, 112
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 4 cut(s) 104, 107, 110, 113
BmiI GGNNCC 1 cut(s) 275
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 3 cut(s) 35, 526, 580
BmtI GCTAGC 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 354
BpiI GAAGAC 3 cut(s) 140, 234, 489
BpmI CTGGAG 2 cut(s) 561, 636
Bpu1102I GCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 2 cut(s) 277, 349
Bse3DI GCAATG 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 2 cut(s) 277, 349
BseSI GKGCMC 1 cut(s) 207
BseYI CCCAGC 2 cut(s) 82, 209
BsgI GTGCAG 1 cut(s) 510
Bsh1236I CGCG 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 273
BsiHKAI GWGCWC 3 cut(s) 50, 284, 371
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 10
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 5 cut(s) 50, 57, 207, 284, 371
Bsp143I GATC 4 cut(s) 339, 356, 393, 583
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 4 cut(s) 347, 364, 401, 591
BspQI GCTCTTC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 2 cut(s) 277, 349
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 4 cut(s) 339, 356, 393, 583
BssT1I CCWWGG 1 cut(s) 466
Bst4CI ACNGT 3 cut(s) 190, 373, 488
Bst6I CTCTTC 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 6 cut(s) 10, 39, 207, 417, 559, 612
BstDEI CTNAG 2 cut(s) 44, 350
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 4 cut(s) 342, 359, 396, 586
BstMBI GATC 4 cut(s) 339, 356, 393, 583
BstMWI GCNNNNNNNGC 3 cut(s) 108, 111, 599
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 102
BstV2I GAAGAC 3 cut(s) 140, 234, 489
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 4 cut(s) 275, 284, 342, 369
Cac8I GCNNGC 6 cut(s) 10, 39, 207, 417, 559, 612
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 12
Csp6I GTAC 1 cut(s) 400
CviAII CATG 1 cut(s) 449
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 44, 350
DpnI GATC 4 cut(s) 341, 358, 395, 585
DpnII GATC 4 cut(s) 339, 356, 393, 583
DraI TTTAAA 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 1 cut(s) 452
FaiI YATR 7 cut(s) 311, 335, 337, 423, 450, 500, 576
FaqI GGGAC 1 cut(s) 449
FatI CATG 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 103, 106, 109, 112
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 103, 106, 109, 112
FspBI CTAG 2 cut(s) 428, 558
GlaI GCGC 1 cut(s) 42
GluI GCNGC 4 cut(s) 103, 106, 109, 112
GsaI CCCAGC 2 cut(s) 86, 213
GsuI CTGGAG 2 cut(s) 561, 636
HapII CCGG 1 cut(s) 13
HhaI GCGC 1 cut(s) 43
Hin1II CATG 1 cut(s) 452
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HinfI GANTC 3 cut(s) 215, 457, 543
HpaII CCGG 1 cut(s) 13
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 4 cut(s) 183, 428, 461, 587
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 67, 70
HpyAV CCTTC 2 cut(s) 136, 573
HpyCH4III ACNGT 3 cut(s) 190, 373, 488
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 3 cut(s) 19, 491, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 111, 599
HpyF3I CTNAG 2 cut(s) 44, 350
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 1 cut(s) 452
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 4 cut(s) 339, 356, 393, 583
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 1 cut(s) 231
LweI GCATC 3 cut(s) 35, 526, 580
MaeI CTAG 2 cut(s) 428, 558
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 461
MalI GATC 4 cut(s) 341, 358, 395, 585
MboI GATC 4 cut(s) 339, 356, 393, 583
MboII GAAGA 8 cut(s) 21, 145, 151, 234, 301, 308, 376, 494
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 5 cut(s) 50, 57, 207, 284, 371
MluCI AATT 2 cut(s) 193, 253
MlyI GAGTC 1 cut(s) 552
MnlI CCTC 5 cut(s) 43, 169, 498, 508, 622
MseI TTAA 1 cut(s) 474
MspI CCGG 1 cut(s) 13
Mva1269I GAATGC 1 cut(s) 10
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 108, 111, 599
NdeII GATC 4 cut(s) 339, 356, 393, 583
NheI GCTAGC 1 cut(s) 557
NlaIII CATG 1 cut(s) 452
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 461
NspV TTCGAA 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 215, 457
PflFI GACNNNGTC 1 cut(s) 440
PkrI GCNGC 4 cut(s) 104, 107, 110, 113
PleI GAGTC 1 cut(s) 551
PpsI GAGTC 1 cut(s) 551
PspFI CCCAGC 2 cut(s) 82, 209
PspN4I GGNNCC 1 cut(s) 275
PsuI RGATCY 1 cut(s) 393
PsyI GACNNNGTC 1 cut(s) 440
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 4 cut(s) 103, 106, 109, 112
Sau3AI GATC 4 cut(s) 339, 356, 393, 583
SchI GAGTC 1 cut(s) 552
SduI GDGCHC 5 cut(s) 50, 57, 207, 284, 371
SfaNI GCATC 3 cut(s) 35, 526, 580
SfuI TTCGAA 1 cut(s) 77
Sse9I AATT 2 cut(s) 193, 253
SsiI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
SspMI CTAG 2 cut(s) 428, 558
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 3 cut(s) 190, 373, 488
TaiI ACGT 1 cut(s) 65
TaqI TCGA 5 cut(s) 57, 77, 182, 245, 257
TasI AATT 2 cut(s) 193, 253
TauI GCSGC 4 cut(s) 105, 108, 111, 114
TfiI GAWTC 2 cut(s) 215, 457
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 4 cut(s) 275, 284, 349, 376
TseFI GTSAC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 4 cut(s) 275, 284, 349, 376
Tth111I GACNNNGTC 1 cut(s) 440
XapI RAATTY 2 cut(s) 193, 253
XbaI TCTAGA 1 cut(s) 427
XspI CTAG 2 cut(s) 428, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.