pycom10g09120

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008976
Physical Location & Seq
Forward (+)
12046004 .. 12048700
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09120.1

Sequence Viewer

Length: 843 bp
ATGATTTTGCGAGACATGCAAGCCAAGTCACTGCAATGGGAGGCGTCTCCGGGGCTCCGGGTCGGGCTCAAAACGCCGCCGTCGTCCTCGTTCTGGTCGCAGCTGGCGGTCGTGAACTTGCGCGGTGGCGCTTCTGTAAATAGCTTGAAAGCCAGAGGTTGGTTTGTAGTAAAGGCCATGGAGGTGGAGGGTTCCAAAACGGCGAGTCTGAATGGGCACGCCGGGAGCTCAGGTACTGGGTTATTTCCTGAGTTGCGCATAAGCCCATTTGCAATTTACCATAAGTTGCTTAAACAAAAGGGCTGTGTCATTTGGATCACCGGTCTCAGCGGGTCAGGGAAGAGCACGGTGGCATGTGCTTTGGGTGGGAGTTTGTACCGCAGGGGGAAGTTGTCGTACATTCTTGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTAGTTTTAAAGCAGAAGATCGTGCAGAGAACATTCGGAGGATTGGTGAGGTGGCGAAATTGTTTGCTGATGCTGGTGTTATTTGTATTGCTAGCTTGATTTCTCCTTATAGAAAGGATCGTGATTCCTGCCGTGCAATGCTACCTGCTGGAGATTTTATTGAGGTGTTCATGGACGTTCCACTTCAAGTTTGTGAAGCTAGAGACCCGAAAGGCCTATATAAGCTTGTACGTGCAGGGAAGATCAAAGTGCAGGTTTTACCGGGATTGATCGATCCATATGAGCCACCACTAAATTGTGAGATAGTATTAACACATAAAGGAGGAGCGTGTCCTTCCCCATGTGAGATGGCCGAAGAAGTGGTTTCTTATTTGGAAGATCAGGGGTTCCTCCAAGCATAA

Protein Analysis

281

Amino Acids

30.35

Weight (kDa)

8.97

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 100 - 251 5.4e-63 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 257
Acc36I ACCTGC 2 cut(s) 595, 685
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 1 cut(s) 123
AciI CCGC 5 cut(s) 77, 107, 123, 330, 379
AclWI GGATC 3 cut(s) 323, 567, 710
AcoI YGGCCR 1 cut(s) 792
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 4 cut(s) 235, 377, 398, 672
AfiI CCNNNNNNNGG 2 cut(s) 93, 159
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 3 cut(s) 148, 433, 629
AluBI AGCT 6 cut(s) 103, 144, 228, 537, 641, 667
AluI AGCT 6 cut(s) 103, 144, 228, 537, 641, 667
Alw21I GWGCWC 2 cut(s) 230, 347
Alw26I GTCTC 4 cut(s) 6, 51, 329, 639
AlwI GGATC 3 cut(s) 323, 567, 710
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 3 cut(s) 174, 655, 792
ApeKI GCWGC 1 cut(s) 100
AsiGI ACCGGT 1 cut(s) 320
AspLEI GCGC 3 cut(s) 123, 131, 258
AsuC2I CCSGG 4 cut(s) 51, 59, 223, 705
AsuHPI GGTGA 2 cut(s) 310, 500
AsuNHI GCTAGC 1 cut(s) 533
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 3 cut(s) 57, 69, 230
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
Bbv12I GWGCWC 2 cut(s) 230, 347
BbvI GCAGC 1 cut(s) 112
BccI CCATC 2 cut(s) 401, 784
BceAI ACGGC 3 cut(s) 64, 216, 558
BcnI CCSGG 4 cut(s) 51, 59, 223, 705
BcoDI GTCTC 4 cut(s) 6, 51, 329, 639
BfaI CTAG 2 cut(s) 534, 642
BfoI RGCGCY 1 cut(s) 132
BfuAI ACCTGC 2 cut(s) 595, 685
BisI GCNGC 2 cut(s) 77, 101
BlsI GCNGC 2 cut(s) 78, 102
Bme1390I CCNGG 4 cut(s) 51, 59, 223, 705
BmiI GGNNCC 3 cut(s) 56, 193, 830
BmrFI CCNGG 4 cut(s) 51, 59, 223, 705
BmrI ACTGGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 502
BmtI GCTAGC 1 cut(s) 537
BmuI ACTGGG 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 1 cut(s) 229
BpuMI CCSGG 4 cut(s) 51, 59, 223, 705
Bsa29I ATCGAT 1 cut(s) 714
BsaAI YACGTR 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 2 cut(s) 329, 639
BsaJI CCNNGG 2 cut(s) 50, 177
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 472, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 159
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 2 cut(s) 41, 585
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 2 cut(s) 50, 177
BseLI CCNNNNNNNGG 2 cut(s) 93, 159
BseMI GCAATG 2 cut(s) 41, 585
BseMII CTCAG 3 cut(s) 240, 243, 340
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 1 cut(s) 780
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 1 cut(s) 112
BsgI GTGCAG 3 cut(s) 486, 696, 713
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 176, 657, 794
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 714
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 230, 347
BsiSI CCGG 5 cut(s) 50, 58, 222, 321, 704
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 93, 159
BsmAI GTCTC 4 cut(s) 6, 51, 329, 639
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 425
BsnI GGCC 3 cut(s) 176, 657, 794
Bso31I GGTCTC 2 cut(s) 329, 639
Bsp1286I GDGCHC 5 cut(s) 57, 69, 219, 230, 347
Bsp143I GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 5 cut(s) 77, 107, 123, 330, 379
BspANI GGCC 3 cut(s) 176, 657, 794
BspCNI CTCAG 3 cut(s) 241, 242, 339
BspDI ATCGAT 1 cut(s) 714
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 56, 193, 830
BspMI ACCTGC 2 cut(s) 595, 685
BspOI GCTAGC 1 cut(s) 537
BspPI GGATC 3 cut(s) 323, 567, 710
BspQI GCTCTTC 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 329, 639
BsrDI GCAATG 2 cut(s) 41, 585
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 50, 177
BssMI GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 335
BstACI GRCGYC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 21, 105, 219, 535
BstDEI CTNAG 4 cut(s) 229, 249, 326, 443
BstDSI CCRYGG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 3 cut(s) 123, 131, 258
BstKTI GATC 7 cut(s) 318, 463, 562, 687, 714, 718, 823
BstMAI GTCTC 4 cut(s) 6, 51, 329, 639
BstMBI GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 2 cut(s) 16, 73
BstNSI RCATGY 2 cut(s) 19, 357
BstSCI CCNGG 4 cut(s) 49, 57, 221, 703
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 112
BstXI CCANNNNNNTGG 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 714
BsuRI GGCC 3 cut(s) 176, 657, 794
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 2 cut(s) 595, 685
Cac8I GCNNGC 4 cut(s) 21, 105, 219, 535
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 3 cut(s) 123, 131, 258
Cfr10I RCCGGY 1 cut(s) 320
ClaI ATCGAT 1 cut(s) 714
CseI GACGC 1 cut(s) 33
Csp6I GTAC 4 cut(s) 234, 376, 397, 671
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 6 cut(s) 16, 178, 354, 425, 613, 783
CviQI GTAC 4 cut(s) 234, 376, 397, 671
DdeI CTNAG 4 cut(s) 229, 249, 326, 443
DpnI GATC 7 cut(s) 317, 462, 561, 686, 713, 717, 822
DpnII GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
DraI TTTAAA 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 792
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 177
Eco147I AGGCCT 1 cut(s) 657
Eco24I GRGCYC 3 cut(s) 57, 69, 230
Eco31I GGTCTC 2 cut(s) 329, 639
Eco53kI GAGCTC 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 3 cut(s) 57, 69, 230
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 6 cut(s) 19, 181, 357, 428, 616, 786
FaqI GGGAC 1 cut(s) 425
FatI CATG 6 cut(s) 15, 177, 353, 424, 612, 782
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 2 cut(s) 77, 101
FriOI GRGCYC 3 cut(s) 57, 69, 230
Fsp4HI GCNGC 2 cut(s) 77, 101
FspBI CTAG 2 cut(s) 534, 642
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 3 cut(s) 122, 130, 257
GluI GCNGC 2 cut(s) 77, 101
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 3 cut(s) 176, 657, 794
HapII CCGG 5 cut(s) 50, 58, 222, 321, 704
HgaI GACGC 1 cut(s) 33
HhaI GCGC 3 cut(s) 123, 131, 258
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 6 cut(s) 19, 181, 357, 428, 616, 786
Hin6I GCGC 3 cut(s) 121, 129, 256
HinP1I GCGC 3 cut(s) 121, 129, 256
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 3 cut(s) 205, 433, 566
HpaII CCGG 5 cut(s) 50, 58, 222, 321, 704
HphI GGTGA 2 cut(s) 310, 500
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 210, 480
Hpy188III TCNNGA 5 cut(s) 112, 248, 404, 437, 563
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 786
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 618, 673
HpyCH4V TGCA 7 cut(s) 19, 34, 272, 467, 578, 677, 694
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 73
HpyF3I CTNAG 4 cut(s) 229, 249, 326, 443
HpySE526I ACGT 2 cut(s) 618, 673
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 6 cut(s) 19, 181, 357, 428, 616, 786
HspAI GCGC 3 cut(s) 121, 129, 256
Kzo9I GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 3 cut(s) 60, 225, 767
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 1 cut(s) 502
MaeI CTAG 2 cut(s) 534, 642
MaeII ACGT 2 cut(s) 618, 673
MaeIII GTNAC 2 cut(s) 27, 437
MalI GATC 7 cut(s) 317, 462, 561, 686, 713, 717, 822
MboI GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
MboII GAAGA 5 cut(s) 352, 470, 694, 809, 830
MhlI GDGCHC 5 cut(s) 57, 69, 219, 230, 347
MluCI AATT 3 cut(s) 273, 500, 736
MlyI GAGTC 1 cut(s) 214
MseI TTAA 3 cut(s) 291, 450, 752
MslI CAYNNNNRTG 2 cut(s) 34, 182
MspA1I CMGCKG 2 cut(s) 103, 330
MspI CCGG 5 cut(s) 50, 58, 222, 321, 704
MspR9I CCNGG 4 cut(s) 51, 59, 223, 705
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 16, 73
NciI CCSGG 4 cut(s) 51, 59, 223, 705
NcoI CCATGG 1 cut(s) 177
NdeI CATATG 1 cut(s) 721
NdeII GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 6 cut(s) 19, 181, 357, 428, 616, 786
NlaIV GGNNCC 3 cut(s) 56, 193, 830
NmuCI GTSAC 2 cut(s) 27, 437
NsbI TGCGCA 1 cut(s) 257
NspI RCATGY 2 cut(s) 19, 357
PceI AGGCCT 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 2 cut(s) 433, 566
PflFI GACNNNGTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 2 cut(s) 78, 102
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Ppu21I YACGTR 1 cut(s) 674
Psp124BI GAGCTC 1 cut(s) 230
PspN4I GGNNCC 3 cut(s) 56, 193, 830
PstNI CAGNNNCTG 1 cut(s) 236
PsyI GACNNNGTC 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 4 cut(s) 235, 377, 398, 672
RsaNI GTAC 4 cut(s) 234, 376, 397, 671
RseI CAYNNNNRTG 2 cut(s) 34, 182
SacI GAGCTC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 3 cut(s) 291, 450, 752
SatI GCNGC 2 cut(s) 77, 101
Sau3AI GATC 7 cut(s) 315, 460, 559, 684, 711, 715, 820
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 4 cut(s) 51, 59, 223, 705
SduI GDGCHC 5 cut(s) 57, 69, 219, 230, 347
SfaNI GCATC 1 cut(s) 502
SmiMI CAYNNNNRTG 2 cut(s) 34, 182
Sse9I AATT 3 cut(s) 273, 500, 736
SseBI AGGCCT 1 cut(s) 657
SsiI CCGC 5 cut(s) 77, 107, 123, 330, 379
SspMI CTAG 2 cut(s) 534, 642
SstI GAGCTC 1 cut(s) 230
StuI AGGCCT 1 cut(s) 657
StyD4I CCNGG 4 cut(s) 49, 57, 221, 703
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 621, 676
TaqI TCGA 1 cut(s) 714
TasI AATT 3 cut(s) 273, 500, 736
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 2 cut(s) 433, 566
Tru1I TTAA 3 cut(s) 291, 450, 752
Tru9I TTAA 3 cut(s) 291, 450, 752
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 2 cut(s) 27, 437
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 2 cut(s) 27, 437
TspDTI ATGAA 1 cut(s) 601
TspRI CASTG 1 cut(s) 36
Tth111I GACNNNGTC 1 cut(s) 416
Van91I CCANNNNNTGG 1 cut(s) 159
XceI RCATGY 2 cut(s) 19, 357
XspI CTAG 2 cut(s) 534, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.