RLG00000014962

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008976
Physical Location & Seq
Reverse (-)
60028664 .. 60031392
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014962

Sequence Viewer

Length: 858 bp
ATGAATGCCAGCCGGTTGCAATGGGCATCTTCCTCGCCTGCGCTGAGCACAGGGCTCGAAACGTCGTCGTCTTGTTCGAAGCTGGGTTTTGTGAACTTTCGCGGTGGCGGTTGTGGAGATAGCTTGAAGACGAAGAAGGGTTTGTTTGTGATAAAGGCGATGGATGTGGAGGGTTCGAGAACCGTGAATTTGGAGAATGGGCACGCTGGGGATTCAGATAAAAATGGGAGCTTGTTGTCTTCGATTGGAAATTCGACCAACATTCAGTGGCACCAGTGCTCGGTGAAGAAAGAAGATAGGGAAAATATGCTGAAACAAAAAGGGTGTGTTATATGGATCACTGGGCTTAGTGGATCAGGGAAGAGCACTGTGGCTTGTGCTTTGAGTGGGATCTTGTTCCGCAGGGGAAAGCTGGCTTATGTTCTAGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGACCGTGCAGAGAACATAAGGAGGATTGGGGAGGTGGCGAAGCTGTTTGCTGATGCTGGAGTCATTTGTATTGCTAGCTTGATTTCTCCTTATAGAAGGGATCGTGATGCCTGTCGTGCGTTATTGCCTGCTGGAGAGTTTATTGAGGTGTTCATGGACATTCCACTCCATGTTTGTGAGGCCAGAGACCCCAAGGGACTATATAAGCTTGCACGTGCAGGGAAGATCAAAGGTTTCACTGGGATTGATGATCCATATGAGTCGCCACTAAATTGTGAGATAGCATTAAGGCATGAAGGAGGAGAGTGTGCATCCCCATGCGAGATGGCCGAAGAAGTGATTTCTTACTTAGAAGAGAAGGGGTTTCTCCAGGCTTAA

Protein Analysis

286

Amino Acids

30.82

Weight (kDa)

7.5

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 107 - 256 2e-70 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 270
AccII CGCG 1 cut(s) 102
AciI CCGC 3 cut(s) 102, 108, 400
AclWI GGATC 5 cut(s) 344, 361, 398, 588, 725
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 2 cut(s) 187, 250
AcvI CACGTG 1 cut(s) 695
AfiI CCNNNNNNNGG 1 cut(s) 280
AgsI TTSAA 2 cut(s) 127, 454
AjnI CCWGG 1 cut(s) 849
AluBI AGCT 7 cut(s) 82, 123, 231, 412, 523, 558, 688
AluI AGCT 7 cut(s) 82, 123, 231, 412, 523, 558, 688
Alw21I GWGCWC 3 cut(s) 50, 281, 368
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 5 cut(s) 344, 361, 398, 588, 725
AoxI GGCC 2 cut(s) 660, 807
ApoI RAATTY 2 cut(s) 187, 250
AspLEI GCGC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 57
BbrPI CACGTG 1 cut(s) 695
BbsI GAAGAC 3 cut(s) 134, 231, 486
Bbv12I GWGCWC 3 cut(s) 50, 281, 368
BccI CCATC 3 cut(s) 154, 422, 799
BcgI CGANNNNNNTGC 4 cut(s) 15, 37, 49, 71
BciT130I CCWGG 1 cut(s) 851
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 2 cut(s) 425, 555
BlpI GCTNAGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 851
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 1 cut(s) 851
BmrI ACTGGG 2 cut(s) 351, 729
BmsI GCATC 4 cut(s) 35, 523, 577, 800
BmtI GCTAGC 1 cut(s) 558
BmuI ACTGGG 2 cut(s) 351, 729
BpiI GAAGAC 3 cut(s) 134, 231, 486
BpmI CTGGAG 3 cut(s) 558, 633, 833
Bpu1102I GCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 463, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 3 cut(s) 274, 346, 724
Bse3DI GCAATG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 2 cut(s) 463, 672
BseGI GGATG 2 cut(s) 169, 791
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 3 cut(s) 274, 346, 724
BseRI GAGGAG 1 cut(s) 795
BseSI GKGCMC 1 cut(s) 204
BseYI CCCAGC 2 cut(s) 82, 206
BsgI GTGCAG 2 cut(s) 507, 717
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 662, 809
BshNI GGYRCC 1 cut(s) 270
BsiHKAI GWGCWC 3 cut(s) 50, 281, 368
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 2 cut(s) 446, 690
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 2 cut(s) 446, 690
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 662, 809
Bso31I GGTCTC 1 cut(s) 660
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 5 cut(s) 50, 57, 204, 281, 368
Bsp143I GATC 6 cut(s) 336, 353, 390, 580, 705, 730
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 3 cut(s) 102, 108, 400
BspANI GGCC 2 cut(s) 662, 809
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 272
BspOI GCTAGC 1 cut(s) 558
BspPI GGATC 5 cut(s) 344, 361, 398, 588, 725
BspQI GCTCTTC 1 cut(s) 356
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 3 cut(s) 274, 346, 724
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 463, 672
BssMI GATC 6 cut(s) 336, 353, 390, 580, 705, 730
BssT1I CCWWGG 2 cut(s) 463, 672
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 3 cut(s) 184, 370, 485
Bst6I CTCTTC 2 cut(s) 356, 828
BstBAI YACGTR 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 7 cut(s) 10, 39, 204, 414, 556, 609, 690
BstDEI CTNAG 3 cut(s) 44, 347, 829
BstF5I GGATG 2 cut(s) 169, 791
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 6 cut(s) 339, 356, 393, 583, 708, 733
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 6 cut(s) 336, 353, 390, 580, 705, 730
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 1 cut(s) 102
BstV2I GAAGAC 3 cut(s) 134, 231, 486
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BsuRI GGCC 2 cut(s) 662, 809
BtgZI GCGATG 1 cut(s) 173
BtsCI GGATG 2 cut(s) 169, 791
BtsIMutI CAGTG 5 cut(s) 272, 281, 339, 366, 717
Cac8I GCNNGC 7 cut(s) 10, 39, 204, 414, 556, 609, 690
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 12
CviAII CATG 5 cut(s) 446, 634, 650, 773, 798
DdeI CTNAG 3 cut(s) 44, 347, 829
DpnI GATC 6 cut(s) 338, 355, 392, 582, 707, 732
DpnII GATC 6 cut(s) 336, 353, 390, 580, 705, 730
DraI TTTAAA 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 807
Eam1104I CTCTTC 2 cut(s) 356, 828
EarI CTCTTC 2 cut(s) 356, 828
Eco130I CCWWGG 2 cut(s) 463, 672
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 660
Eco72I CACGTG 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 2 cut(s) 463, 672
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 463, 672
FaeI CATG 5 cut(s) 449, 637, 653, 776, 801
FaqI GGGAC 2 cut(s) 446, 690
FatI CATG 5 cut(s) 445, 633, 649, 772, 797
FauNDI CATATG 1 cut(s) 736
FokI GGATG 2 cut(s) 176, 778
FriOI GRGCYC 1 cut(s) 57
FspBI CTAG 2 cut(s) 425, 555
GlaI GCGC 1 cut(s) 42
GsaI CCCAGC 2 cut(s) 86, 210
GsuI CTGGAG 3 cut(s) 558, 633, 833
HaeIII GGCC 2 cut(s) 662, 809
HapII CCGG 1 cut(s) 13
HhaI GCGC 1 cut(s) 43
Hin1II CATG 5 cut(s) 449, 637, 653, 776, 801
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 4 cut(s) 212, 454, 540, 740
HpaII CCGG 1 cut(s) 13
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 4 cut(s) 177, 425, 458, 584
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 67, 70
HpyAV CCTTC 4 cut(s) 130, 570, 770, 832
HpyCH4III ACNGT 3 cut(s) 184, 370, 485
HpyCH4IV ACGT 2 cut(s) 62, 694
HpyCH4V TGCA 5 cut(s) 19, 488, 692, 698, 791
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 3 cut(s) 44, 347, 829
HpySE526I ACGT 2 cut(s) 62, 694
Hsp92II CATG 5 cut(s) 449, 637, 653, 776, 801
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 6 cut(s) 336, 353, 390, 580, 705, 730
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 1 cut(s) 228
LweI GCATC 4 cut(s) 35, 523, 577, 800
MaeI CTAG 2 cut(s) 425, 555
MaeII ACGT 2 cut(s) 62, 694
MaeIII GTNAC 1 cut(s) 458
MalI GATC 6 cut(s) 338, 355, 392, 582, 707, 732
MboI GATC 6 cut(s) 336, 353, 390, 580, 705, 730
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 5 cut(s) 50, 57, 204, 281, 368
MluCI AATT 3 cut(s) 187, 250, 751
MlyI GAGTC 2 cut(s) 549, 749
MnlI CCTC 7 cut(s) 43, 163, 495, 505, 619, 652, 773
MseI TTAA 3 cut(s) 471, 767, 856
MslI CAYNNNNRTG 2 cut(s) 654, 796
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 851
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 596
NdeI CATATG 1 cut(s) 736
NdeII GATC 6 cut(s) 336, 353, 390, 580, 705, 730
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 5 cut(s) 449, 637, 653, 776, 801
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 458
NspV TTCGAA 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 212, 454
PflFI GACNNNGTC 1 cut(s) 437
PleI GAGTC 2 cut(s) 548, 748
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 2 cut(s) 548, 748
Ppu21I YACGTR 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 849
PspCI CACGTG 1 cut(s) 695
PspFI CCCAGC 2 cut(s) 82, 206
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 1 cut(s) 272
PsuI RGATCY 1 cut(s) 390
PsyI GACNNNGTC 1 cut(s) 437
RseI CAYNNNNRTG 2 cut(s) 654, 796
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 3 cut(s) 471, 767, 856
Sau3AI GATC 6 cut(s) 336, 353, 390, 580, 705, 730
SchI GAGTC 2 cut(s) 549, 749
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 5 cut(s) 50, 57, 204, 281, 368
SfaNI GCATC 4 cut(s) 35, 523, 577, 800
SfuI TTCGAA 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 654, 796
Sse9I AATT 3 cut(s) 187, 250, 751
SsiI CCGC 3 cut(s) 102, 108, 400
SspMI CTAG 2 cut(s) 425, 555
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 2 cut(s) 463, 672
TaaI ACNGT 3 cut(s) 184, 370, 485
TaiI ACGT 2 cut(s) 65, 697
TaqI TCGA 5 cut(s) 57, 77, 176, 242, 254
TasI AATT 3 cut(s) 187, 250, 751
TfiI GAWTC 2 cut(s) 212, 454
Tru1I TTAA 3 cut(s) 471, 767, 856
Tru9I TTAA 3 cut(s) 471, 767, 856
TscAI CASTG 5 cut(s) 272, 281, 346, 373, 724
TseFI GTSAC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 17, 622, 789
TspRI CASTG 5 cut(s) 272, 281, 346, 373, 724
Tth111I GACNNNGTC 1 cut(s) 437
XapI RAATTY 2 cut(s) 187, 250
XbaI TCTAGA 1 cut(s) 424
XspI CTAG 2 cut(s) 425, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.