Rorug05G0548100

Adenylyl-sulfate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008976
Physical Location & Seq
Reverse (-)
73714136 .. 73715965
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0548100.1

Sequence Viewer

Length: 384 bp
ATGGGGTTCTTCCCAATCCTATCGCATCCTGTTTTAGCTGCACCAATGCCGGAGATGAAGGATCCTGAAGTGATTCGGACCTTAAAGCTTCCCAGTGGTGTGAGGATCCAAGCATATGAATTCTTGAGAATGTTAGCACTCCAGACTTCATTTGAATACGCCATCAAGTCTTACATAATTCCTATTCAGATTATAAAAGGCTTGGAGGAGGTTCTGGTTGGCATGAGAATTGGAGGAAAAAGGAGAGCATTGATACCTCCTTCTGTGGGATACACAACTGAAAGCTTACAACCAATTCCTGAAGAGTTTGGCCCTCGACGCAGCCTCTTGTCTCATGTAAATGAGCCTCTGATATTCGAGGTGCAGCTCTTGAAACTACTTTGA

Protein Analysis

127

Amino Acids

14.34

Weight (kDa)

9.05

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKBP_C PF00254 63 - 124 9.9e-10 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 194
AclWI GGATC 4 cut(s) 56, 69, 100, 113
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 2 cut(s) 87, 321
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 155, 373
AluBI AGCT 4 cut(s) 38, 88, 285, 367
AluI AGCT 4 cut(s) 38, 88, 285, 367
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 4 cut(s) 56, 69, 100, 113
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 3 cut(s) 38, 321, 364
ApoI RAATTY 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 78, 311
AvaII GGWCC 1 cut(s) 78
BamHI GGATCC 2 cut(s) 61, 105
BbvI GCAGC 3 cut(s) 25, 333, 376
BccI CCATC 1 cut(s) 170
BciVI GTATCC 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 336
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 3 cut(s) 39, 322, 365
BlsI GCNGC 3 cut(s) 40, 323, 366
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 78, 311
BmiI GGNNCC 2 cut(s) 63, 107
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 34
BmuI ACTGGG 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 3 cut(s) 25, 333, 376
BsgI GTGCAG 2 cut(s) 24, 383
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 336
BsnI GGCC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 61, 105
BspANI GGCC 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 63, 107
BspPI GGATC 4 cut(s) 56, 69, 100, 113
BsrI ACTGG 1 cut(s) 93
BssMI GATC 2 cut(s) 61, 105
Bst6I CTCTTC 1 cut(s) 297
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 2 cut(s) 64, 108
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 2 cut(s) 61, 105
BstMWI GCNNNNNNNGC 1 cut(s) 318
BstV1I GCAGC 3 cut(s) 25, 333, 376
BstX2I RGATCY 2 cut(s) 61, 105
BstYI RGATCY 2 cut(s) 61, 105
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 78, 311
CseI GACGC 1 cut(s) 327
CviAII CATG 2 cut(s) 223, 335
CviJI RGCY 8 cut(s) 38, 88, 201, 285, 312, 324, 346, 367
CviKI_1 RGCY 8 cut(s) 38, 88, 201, 285, 312, 324, 346, 367
DpnI GATC 2 cut(s) 63, 107
DpnII GATC 2 cut(s) 61, 105
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 78
Eco57I CTGAAG 2 cut(s) 87, 321
EcoRI GAATTC 1 cut(s) 119
FaeI CATG 2 cut(s) 226, 338
FaiI YATR 6 cut(s) 115, 117, 176, 194, 224, 336
FatI CATG 2 cut(s) 222, 334
FauNDI CATATG 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 39, 322, 365
FokI GGATG 1 cut(s) 12
Fsp4HI GCNGC 3 cut(s) 39, 322, 365
GluI GCNGC 3 cut(s) 39, 322, 365
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 327
Hin1II CATG 2 cut(s) 226, 338
HindIII AAGCTT 2 cut(s) 86, 283
HinfI GANTC 1 cut(s) 73
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 3 cut(s) 78, 189, 351
Hpy188III TCNNGA 5 cut(s) 65, 124, 142, 299, 370
Hpy99I CGWCG 1 cut(s) 321
HpyAV CCTTC 2 cut(s) 52, 270
HpyCH4V TGCA 2 cut(s) 41, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 318
Hsp92II CATG 2 cut(s) 226, 338
Kzo9I GATC 2 cut(s) 61, 105
LpnPI CCDG 7 cut(s) 42, 63, 78, 106, 155, 200, 312
Lsp1109I GCAGC 3 cut(s) 25, 333, 376
LweI GCATC 1 cut(s) 34
MalI GATC 2 cut(s) 63, 107
MboI GATC 2 cut(s) 61, 105
MboII GAAGA 1 cut(s) 314
MflI RGATCY 2 cut(s) 61, 105
MluCI AATT 4 cut(s) 119, 177, 228, 294
MnlI CCTC 9 cut(s) 96, 199, 202, 227, 267, 324, 335, 352, 357
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 318
NdeI CATATG 1 cut(s) 115
NdeII GATC 2 cut(s) 61, 105
NlaIII CATG 2 cut(s) 226, 338
NlaIV GGNNCC 2 cut(s) 63, 107
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 73
PkrI GCNGC 3 cut(s) 40, 323, 366
PsiI TTATAA 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 63, 107
PspPI GGNCC 2 cut(s) 78, 311
PsuI RGATCY 2 cut(s) 61, 105
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 3 cut(s) 39, 322, 365
Sau3AI GATC 2 cut(s) 61, 105
Sau96I GGNCC 2 cut(s) 78, 311
SetI ASST 8 cut(s) 40, 83, 90, 213, 259, 287, 363, 369
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 4 cut(s) 119, 177, 228, 294
TaqI TCGA 2 cut(s) 316, 357
TasI AATT 4 cut(s) 119, 177, 228, 294
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 1 cut(s) 100
TseI GCWGC 3 cut(s) 38, 321, 364
TspDTI ATGAA 3 cut(s) 71, 132, 138
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 78
XapI RAATTY 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.