MD05G1155500.v1.1

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010302
Physical Location & Seq
Reverse (-)
28545967 .. 28548517
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1155500.v1.1.491

Sequence Viewer

Length: 870 bp
ATGAGCGAAGTATCTCCCTTCAAGTTATTCAGGACTTTCAGTGATGCATTTCTTCGCCGCAGTGGATCGTTCACCTGCCAACACCCTGGAGTTCGAGCTCAAACGCTTCAACCGCCCGCCACCTCCCCAAAACCAGAAGAACAAAAAAGGAGCGAACTTTCTGCTTCCTCTCTATTGCAGAAGCCAAGCAAACTGTTGGGTCGCGATCTCTCTGTCACAGCAATGGATGATGAAGAACCTTCTATGAGCAAGGATGAGGACGTGTTAGAGAGTTCCAATGGGAAAGAGGCAGTCACATATGAGGGGAACTCGGATACAGAACCATTTGTGGGTATGGAGTTTGAATATGAAGAGGCTGCGAAAGTGTTCTATGATGCATATGCAACACAGGTGGGCTTCATTATGCGAGTCGATGCCTTCCGGAGATCAATGCGTGATGGTAAGGTAGTTTGGCGGAGGCTTGTGTGTAACAAAGAGGGATTTCGCATGACGCGAGCCAAAAGAACTGAGAATAGGAAGCCTCGAGCAATCACCAGAGAAGGGTGCAAAGCGATGCTTGTGGTAAAGAAAGACAAATCAGGAAAATGGATCGTAACACGATTTATAAAGGAGCATAACCGTCCACTAGTAGGTACACCTGCAAATGGTCGCCGCAGTATGCTTCTGTCTCAAACACCAGACGAAAAATATCTGAAAATTCGGGAGTTGACGGCTGAGTTGCAACGTGAGCGAAAGATGTCTGCCACCTATCAAGAACAGCTTGATATGGTTTTGAGGCAGATGGAAGAACATTCCAATCACTTGTCGAGAAACATTGACGGTATAGTCCAGAGCGTGAAAGAAATTGAATCAAAGAGGGTAGCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

290

Amino Acids

33.15

Weight (kDa)

9.4

Isoelectric Point (pI)

61.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 123 - 210 2.6e-30 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AarI CACCTGC 2 cut(s) 83, 646
AasI GACNNNNNNGTC 1 cut(s) 824
Acc36I ACCTGC 2 cut(s) 83, 646
AccII CGCG 2 cut(s) 204, 493
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 5 cut(s) 58, 113, 117, 454, 652
AclWI GGATC 2 cut(s) 73, 596
AcsI RAATTY 1 cut(s) 696
AfaI GTAC 1 cut(s) 634
AfiI CCNNNNNNNGG 4 cut(s) 329, 540, 629, 644
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 4 cut(s) 22, 110, 344, 848
AhlI ACTAGT 1 cut(s) 625
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 98, 760
AluI AGCT 2 cut(s) 98, 760
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 1 cut(s) 672
AlwI GGATC 2 cut(s) 73, 596
Ama87I CYCGRG 1 cut(s) 522
Aor13HI TCCGGA 1 cut(s) 420
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 696
Asp700I GAANNNNTTC 1 cut(s) 365
AsuHPI GGTGA 2 cut(s) 64, 523
AvaI CYCGRG 1 cut(s) 522
BanII GRGCYC 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 431, 775
BceAI ACGGC 1 cut(s) 726
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 1 cut(s) 87
BciVI GTATCC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 672
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 1 cut(s) 626
BfuAI ACCTGC 2 cut(s) 83, 646
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 3 cut(s) 58, 357, 652
BlsI GCNGC 3 cut(s) 59, 358, 653
Bme1390I CCNGG 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 522
BmgBI CACGTC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 34, 364, 403, 543
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 420
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 540, 629, 644
Bse3DI GCAATG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 2 cut(s) 232, 259
BseLI CCNNNNNNNGG 4 cut(s) 329, 540, 629, 644
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 498, 705
BseXI GCAGC 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 204, 493
BsiHKAI GWGCWC 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 522
BsiSI CCGG 1 cut(s) 421
BslI CCNNNNNNNGG 4 cut(s) 329, 540, 629, 644
BsmAI GTCTC 1 cut(s) 672
BsoBI CYCGRG 1 cut(s) 522
Bsp1286I GDGCHC 1 cut(s) 100
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 4 cut(s) 65, 205, 425, 588
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 5 cut(s) 58, 113, 117, 454, 652
BspCNI CTCAG 2 cut(s) 499, 706
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 2 cut(s) 204, 493
BspMI ACCTGC 2 cut(s) 83, 646
BspPI GGATC 2 cut(s) 73, 596
BsrDI GCAATG 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 4 cut(s) 65, 205, 425, 588
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 195, 620, 821
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 117, 495
BstDEI CTNAG 2 cut(s) 507, 714
BstF5I GGATG 2 cut(s) 232, 259
BstFNI CGCG 2 cut(s) 204, 493
BstKTI GATC 4 cut(s) 68, 208, 428, 591
BstMAI GTCTC 1 cut(s) 672
BstMBI GATC 4 cut(s) 65, 205, 425, 588
BstMWI GCNNNNNNNGC 2 cut(s) 112, 727
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstUI CGCG 2 cut(s) 204, 493
BstV1I GCAGC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 86
BsuI GTATCC 1 cut(s) 307
BtgZI GCGATG 1 cut(s) 566
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 2 cut(s) 232, 259
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 46, 67
BtuMI TCGCGA 1 cut(s) 204
BveI ACCTGC 2 cut(s) 83, 646
Cac8I GCNNGC 2 cut(s) 117, 495
CseI GACGC 1 cut(s) 499
Csp6I GTAC 1 cut(s) 633
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 1 cut(s) 487
CviJI RGCY 9 cut(s) 98, 184, 356, 396, 460, 497, 520, 713, 760
CviKI_1 RGCY 9 cut(s) 98, 184, 356, 396, 460, 497, 520, 713, 760
CviQI GTAC 1 cut(s) 633
DdeI CTNAG 2 cut(s) 507, 714
DpnI GATC 4 cut(s) 67, 207, 427, 590
DpnII GATC 4 cut(s) 65, 205, 425, 588
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 469
Ecl136II GAGCTC 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 85
EcoT22I ATGCAT 2 cut(s) 49, 379
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 1 cut(s) 490
FalI AAGNNNNNCTT 4 cut(s) 540, 572, 744, 776
FatI CATG 1 cut(s) 486
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 2 cut(s) 298, 379
Fnu4HI GCNGC 3 cut(s) 58, 357, 652
FokI GGATG 2 cut(s) 239, 266
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 3 cut(s) 58, 357, 652
FspBI CTAG 1 cut(s) 626
GluI GCNGC 3 cut(s) 58, 357, 652
GsuI CTGGAG 1 cut(s) 108
HapII CCGG 1 cut(s) 421
HgaI GACGC 1 cut(s) 499
Hin1II CATG 1 cut(s) 490
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HinfI GANTC 2 cut(s) 408, 848
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 64, 523
Hpy166II GTNNAC 4 cut(s) 72, 623, 635, 708
Hpy188I TCNGA 2 cut(s) 313, 693
Hpy188III TCNNGA 8 cut(s) 31, 203, 421, 579, 701, 752, 807, 829
Hpy8I GTNNAC 4 cut(s) 72, 623, 635, 708
HpyAV CCTTC 4 cut(s) 28, 249, 427, 533
HpyCH4III ACNGT 3 cut(s) 195, 620, 821
HpyCH4IV ACGT 2 cut(s) 261, 724
HpyCH4V TGCA 7 cut(s) 47, 178, 377, 383, 546, 641, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 727
HpyF3I CTNAG 2 cut(s) 507, 714
HpySE526I ACGT 2 cut(s) 261, 724
Hsp92II CATG 1 cut(s) 490
Kpn2I TCCGGA 1 cut(s) 420
Kzo9I GATC 4 cut(s) 65, 205, 425, 588
LmnI GCTCC 2 cut(s) 150, 610
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 4 cut(s) 34, 364, 403, 543
MaeI CTAG 1 cut(s) 626
MaeII ACGT 2 cut(s) 261, 724
MaeIII GTNAC 4 cut(s) 214, 292, 467, 592
MalI GATC 4 cut(s) 67, 207, 427, 590
MboI GATC 4 cut(s) 65, 205, 425, 588
MboII GAAGA 5 cut(s) 44, 149, 245, 362, 797
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 2 cut(s) 696, 843
MlyI GAGTC 1 cut(s) 417
Mph1103I ATGCAT 2 cut(s) 49, 379
MroI TCCGGA 1 cut(s) 420
MroXI GAANNNNTTC 1 cut(s) 365
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 2 cut(s) 204, 493
MwoI GCNNNNNNNGC 2 cut(s) 112, 727
NdeI CATATG 2 cut(s) 298, 379
NdeII GATC 4 cut(s) 65, 205, 425, 588
NlaIII CATG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 214, 292
NruI TCGCGA 1 cut(s) 204
NsiI ATGCAT 2 cut(s) 49, 379
PaeR7I CTCGAG 1 cut(s) 522
PaqCI CACCTGC 2 cut(s) 83, 646
PcsI WCGNNNNNNNCGW 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 848
PkrI GCNGC 3 cut(s) 59, 358, 653
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PsiI TTATAA 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspXI VCTCGAGB 1 cut(s) 522
RruI TCGCGA 1 cut(s) 204
RsaI GTAC 1 cut(s) 634
RsaNI GTAC 1 cut(s) 633
RseI CAYNNNNRTG 1 cut(s) 221
SacI GAGCTC 1 cut(s) 100
SatI GCNGC 3 cut(s) 58, 357, 652
Sau3AI GATC 4 cut(s) 65, 205, 425, 588
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 4 cut(s) 34, 364, 403, 543
Sfr274I CTCGAG 1 cut(s) 522
SlaI CTCGAG 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 522
SmoI CTYRAG 1 cut(s) 522
SpeI ACTAGT 1 cut(s) 625
Sse9I AATT 2 cut(s) 696, 843
SsiI CCGC 5 cut(s) 58, 113, 117, 454, 652
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 3 cut(s) 195, 620, 821
TaiI ACGT 2 cut(s) 264, 727
TaqI TCGA 4 cut(s) 94, 411, 523, 806
TasI AATT 2 cut(s) 696, 843
TauI GCSGC 2 cut(s) 60, 654
TfiI GAWTC 1 cut(s) 848
TscAI CASTG 2 cut(s) 46, 67
TseFI GTSAC 2 cut(s) 214, 292
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 214, 292
TspDTI ATGAA 3 cut(s) 246, 363, 388
TspRI CASTG 2 cut(s) 46, 67
XapI RAATTY 1 cut(s) 696
XhoI CTCGAG 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 1 cut(s) 626
Zsp2I ATGCAT 2 cut(s) 49, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.