MD10G1149900.v1.1

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010302
Physical Location & Seq
Reverse (-)
23706081 .. 23708885
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1149900.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAGCAAGGATGAGGACGTGTTAGAGAGTTCCAATGGGAAAGATGCAGTCACATCTGAGGGGAACTCGGATGCGGAACCATTTGTGGGTATGGAGTTTGAAACTGAAGAGGCTGCCAAGGTGTTCTATGATGCGTATGCAACACAGGTGGGTTTCATTATGCGAGTTGATGCCTTCCGGAGATCAATGCGCGATGGTAAGGTAGTTTGGCGTAGGCTTGTGTGTAATAAAGAGGGATTTCGCAAGACGCGAGCCAAAAGAACTGAGAACAGGAAGCCTCGAGCAATCACCAGAGAAGGGTGCAAAGCAATGATTGTGGTAAAGAAAGACAAGTCAGGGAAATGGGTCGTAACACGATTTATAAAGGAGCATAACCATCCACTAGTAGGTACACCTGCAAATGGTCGCCGCAGTATGCTTCTGTCTCAAGCACCAGATGAAAAAGACCTGAAAATTCGGGAGTTGACTGCTGAGCTACAACGTGAGCGAAAAAGGTCTGCCACCTATCAAGAACAGCTTGATATGGTTTTGAGGGAGATGGAACAGCATTCCAATCACCTGTCGAGAAACATCGACGGTATAGTCCAAAGCGTGAAAGAAATCGAATCAAAGAGGGTAGCACCTTCAAACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

212

Amino Acids

24.19

Weight (kDa)

9.51

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 42 - 129 3e-33 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AarI CACCTGC 1 cut(s) 403
AasI GACNNNNNNGTC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 403
AccII CGCG 2 cut(s) 192, 250
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 2 cut(s) 74, 409
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 5 cut(s) 86, 297, 386, 401, 629
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 2 cut(s) 101, 627
AhlI ACTAGT 1 cut(s) 382
AjiI CACGTC 1 cut(s) 19
AluBI AGCT 2 cut(s) 475, 517
AluI AGCT 2 cut(s) 475, 517
Alw26I GTCTC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 279
Aor13HI TCCGGA 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 453
AspLEI GCGC 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 280, 548
AvaI CYCGRG 1 cut(s) 279
BbvI GCAGC 1 cut(s) 100
BccI CCATC 3 cut(s) 188, 384, 532
BcoDI GTCTC 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 403
BisI GCNGC 2 cut(s) 114, 409
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 2 cut(s) 115, 410
BmeT110I CYCGRG 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 78
BmsI GCATC 4 cut(s) 34, 61, 121, 160
BplI GAGNNNNNCTC 2 cut(s) 50, 82
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 297, 386, 401, 629
Bse3DI GCAATG 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 3 cut(s) 16, 76, 376
BseLI CCNNNNNNNGG 5 cut(s) 86, 297, 386, 401, 629
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 3 cut(s) 48, 255, 462
BseXI GCAGC 1 cut(s) 100
Bsh1236I CGCG 2 cut(s) 192, 250
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 5 cut(s) 86, 297, 386, 401, 629
BsmAI GTCTC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 177
Bsp143I GATC 1 cut(s) 182
Bsp1720I GCTNAGC 1 cut(s) 471
BspACI CCGC 2 cut(s) 74, 409
BspCNI CTCAG 3 cut(s) 49, 256, 463
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 2 cut(s) 192, 250
BspLI GGNNCC 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 578, 632
Bst6I CTCTTC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 3 cut(s) 57, 264, 471
BstF5I GGATG 3 cut(s) 16, 76, 376
BstFNI CGCG 2 cut(s) 192, 250
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 1 cut(s) 185
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 1 cut(s) 182
BstUI CGCG 2 cut(s) 192, 250
BstV1I GCAGC 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 3 cut(s) 16, 76, 376
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 1 cut(s) 192
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 390
CspCI CAANNNNNGTGG 4 cut(s) 129, 164, 297, 332
CviJI RGCY 6 cut(s) 113, 217, 254, 277, 475, 517
CviKI_1 RGCY 6 cut(s) 113, 217, 254, 277, 475, 517
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 3 cut(s) 57, 264, 471
DpnI GATC 1 cut(s) 184
DpnII GATC 1 cut(s) 182
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaiI YATR 9 cut(s) 92, 129, 138, 161, 362, 372, 416, 524, 581
FalI AAGNNNNNCTT 2 cut(s) 501, 533
Fnu4HI GCNGC 2 cut(s) 114, 409
FokI GGATG 3 cut(s) 23, 83, 363
Fsp4HI GCNGC 2 cut(s) 114, 409
FspBI CTAG 1 cut(s) 383
GlaI GCGC 1 cut(s) 191
GluI GCNGC 2 cut(s) 114, 409
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 192
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HinfI GANTC 1 cut(s) 605
HpaII CCGG 1 cut(s) 178
HphI GGTGA 2 cut(s) 280, 548
Hpy166II GTNNAC 2 cut(s) 392, 465
Hpy188I TCNGA 2 cut(s) 58, 70
Hpy188III TCNNGA 4 cut(s) 178, 458, 509, 564
Hpy8I GTNNAC 2 cut(s) 392, 465
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 3 cut(s) 184, 290, 633
HpyCH4III ACNGT 2 cut(s) 578, 632
HpyCH4IV ACGT 2 cut(s) 18, 481
HpyCH4V TGCA 4 cut(s) 47, 140, 303, 398
HpyF3I CTNAG 3 cut(s) 57, 264, 471
HpySE526I ACGT 2 cut(s) 18, 481
HspAI GCGC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 177
Kzo9I GATC 1 cut(s) 182
LmnI GCTCC 1 cut(s) 367
LpnPI CCDG 9 cut(s) 131, 191, 256, 304, 321, 408, 447, 461, 572
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 4 cut(s) 34, 61, 121, 160
MaeI CTAG 1 cut(s) 383
MaeII ACGT 2 cut(s) 18, 481
MaeIII GTNAC 2 cut(s) 49, 349
MalI GATC 1 cut(s) 184
MboI GATC 1 cut(s) 182
MboII GAAGA 1 cut(s) 119
MluCI AATT 1 cut(s) 453
MnlI CCTC 7 cut(s) 7, 52, 103, 226, 288, 525, 606
MroI TCCGGA 1 cut(s) 177
MseI TTAA 1 cut(s) 634
MspI CCGG 1 cut(s) 178
Mva1269I GAATGC 1 cut(s) 547
MvnI CGCG 2 cut(s) 192, 250
NdeII GATC 1 cut(s) 182
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 279
PaqCI CACCTGC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 2 cut(s) 115, 410
PsiI TTATAA 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 78
PspXI VCTCGAGB 1 cut(s) 279
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 634
SatI GCNGC 2 cut(s) 114, 409
Sau3AI GATC 1 cut(s) 182
SfaNI GCATC 4 cut(s) 34, 61, 121, 160
Sfr274I CTCGAG 1 cut(s) 279
SlaI CTCGAG 1 cut(s) 279
SmlI CTYRAG 2 cut(s) 279, 426
SmoI CTYRAG 2 cut(s) 279, 426
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 1 cut(s) 453
SsiI CCGC 2 cut(s) 74, 409
SspMI CTAG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 578, 632
TaiI ACGT 2 cut(s) 21, 484
TaqI TCGA 4 cut(s) 280, 563, 573, 603
TasI AATT 1 cut(s) 453
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 1 cut(s) 634
Tru9I TTAA 1 cut(s) 634
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 2 cut(s) 145, 453
XapI RAATTY 1 cut(s) 453
XhoI CTCGAG 1 cut(s) 279
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.