MD05G1231300.v1.1
ERF Family

2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001917
Physical Location & Seq
Forward (+)
36474548 .. 36475655
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1231300.v1.1.491

Sequence Viewer

Length: 711 bp
ATGACCCCCGTGACTGGTCCTCAAGAAGCTATTGTTTACCCTGGACTCGCACTCTATCAGGCAACTGCAGGATATGTTAACCCTGCACTGCAGAGAACGGAGACTGCTAATACACAGAGCAACTTGTTTGGGCGATGTTCTTTGGCTTTTAAATTCATGCCTAAATCCATGACCAATCTTAGTTGTTCAGAGATGAGAACTGCTGGTCATGGGGTTGAAGCTCAGTTCCAGCTTCCAGTAGCGGTTGATGACTTTATGCAGAGATCTCACCCCACCAATCAACTCTTTAACAGACACGGTTTCCAAAGTTCCAATTTCCATGCAGCACAAGATGGATCTATGAAGCCTTTGTTGAGGAGGAGAAAGGGTGAAAGAACCAAACCTCTGCCACCTTCCAAGAGATTGTGGGTCGAGGTCCAGAGAGTCCTGATGGAAAGGGTTCAAGACATTGCAGACAAGAAGGGAATCAAGCTGAGGTTCTGCAACCTGAAGGAGTGTGAGAATCACATTCAGACACTTGATAGCCCCTTTGCCAATATAAGAATGGAGATTGGGTGGCCAAACGAGGTGCCATTTGTTCATTCCCATGATCTCCCCGACAAGGCAAAGATCGGTTTCCTTGAAGCCTATGAACCTGGTTGGACAGCTACTCATGATATGGAGTTAAGTCTATCTGAACCTGGACAGGCTGGCCAGTCAACACTCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.48

Weight (kDa)

8.35

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 343
AcoI YGGCCR 2 cut(s) 557, 691
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 509
AfiI CCNNNNNNNGG 2 cut(s) 14, 601
AgsI TTSAA 3 cut(s) 218, 443, 623
AjnI CCWGG 3 cut(s) 40, 634, 679
AluBI AGCT 5 cut(s) 29, 221, 232, 472, 647
AluI AGCT 5 cut(s) 29, 221, 232, 472, 647
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 1 cut(s) 343
AoxI GGCC 2 cut(s) 557, 691
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 17, 415
AsuHPI GGTGA 2 cut(s) 260, 380
AvaII GGWCC 2 cut(s) 17, 415
BalI TGGCCA 2 cut(s) 559, 693
BanI GGYRCC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 335
BccI CCATC 2 cut(s) 326, 424
BciT130I CCWGG 3 cut(s) 42, 636, 681
BcoDI GTCTC 1 cut(s) 95
BfmI CTRYAG 2 cut(s) 66, 89
BglII AGATCT 1 cut(s) 263
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
Bme1390I CCNGG 3 cut(s) 42, 636, 681
Bme18I GGWCC 2 cut(s) 17, 415
BmgT120I GGNCC 2 cut(s) 17, 415
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 3 cut(s) 42, 636, 681
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 40
BsaXI ACNNNNNCTCC 4 cut(s) 352, 382, 539, 569
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 601
Bse1I ACTGG 3 cut(s) 19, 236, 694
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 3 cut(s) 42, 636, 681
BseDI CCNNGG 1 cut(s) 40
BseLI CCNNNNNNNGG 2 cut(s) 14, 601
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 2 cut(s) 236, 464
BseNI ACTGG 3 cut(s) 19, 236, 694
BseRI GAGGAG 2 cut(s) 370, 373
BseXI GCAGC 1 cut(s) 335
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 2 cut(s) 559, 693
BshNI GGYRCC 1 cut(s) 568
BslI CCNNNNNNNGG 2 cut(s) 14, 601
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 2 cut(s) 559, 693
Bsp143I GATC 4 cut(s) 263, 335, 589, 609
BspACI CCGC 1 cut(s) 242
BspANI GGCC 2 cut(s) 559, 693
BspCNI CTCAG 2 cut(s) 235, 465
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 570
BspMAI CTGCAG 2 cut(s) 70, 93
BspPI GGATC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 447
BsrI ACTGG 3 cut(s) 19, 236, 694
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 263, 335, 589, 609
Bst2UI CCWGG 3 cut(s) 42, 636, 681
Bst4CI ACNGT 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 691
BstDEI CTNAG 3 cut(s) 179, 222, 473
BstKTI GATC 4 cut(s) 266, 338, 592, 612
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 4 cut(s) 263, 335, 589, 609
BstNI CCWGG 3 cut(s) 42, 636, 681
BstSCI CCNGG 3 cut(s) 40, 634, 679
BstSFI CTRYAG 2 cut(s) 66, 89
BstV1I GCAGC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 263, 335
BstYI RGATCY 2 cut(s) 263, 335
BsuRI GGCC 2 cut(s) 559, 693
BtgZI GCGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 1 cut(s) 86
Cac8I GCNNGC 1 cut(s) 691
CciI TCATGA 1 cut(s) 652
Cfr13I GGNCC 2 cut(s) 17, 415
CsiI ACCWGGT 1 cut(s) 634
CviAII CATG 6 cut(s) 157, 169, 209, 320, 587, 653
DdeI CTNAG 3 cut(s) 179, 222, 473
DpnI GATC 4 cut(s) 265, 337, 591, 611
DpnII GATC 4 cut(s) 263, 335, 589, 609
DraI TTTAAA 1 cut(s) 151
EaeI YGGCCR 2 cut(s) 557, 691
Eco47I GGWCC 2 cut(s) 17, 415
Eco57I CTGAAG 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 40, 634, 679
FaeI CATG 6 cut(s) 160, 172, 212, 323, 590, 656
FatI CATG 6 cut(s) 156, 168, 208, 319, 586, 652
Fnu4HI GCNGC 1 cut(s) 324
Fsp4HI GCNGC 1 cut(s) 324
GluI GCNGC 1 cut(s) 324
HaeIII GGCC 2 cut(s) 559, 693
Hin1II CATG 6 cut(s) 160, 172, 212, 323, 590, 656
HincII GTYRAC 2 cut(s) 79, 699
HindII GTYRAC 2 cut(s) 79, 699
HinfI GANTC 4 cut(s) 45, 423, 465, 502
HpaI GTTAAC 1 cut(s) 79
HphI GGTGA 2 cut(s) 260, 380
Hpy166II GTNNAC 3 cut(s) 37, 79, 699
Hpy188I TCNGA 3 cut(s) 190, 513, 676
Hpy188III TCNNGA 5 cut(s) 23, 418, 427, 443, 653
Hpy8I GTNNAC 3 cut(s) 37, 79, 699
HpyAV CCTTC 3 cut(s) 402, 454, 484
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 7 cut(s) 68, 86, 91, 259, 323, 452, 483
HpyF3I CTNAG 3 cut(s) 179, 222, 473
Hsp92II CATG 6 cut(s) 160, 172, 212, 323, 590, 656
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 263, 335, 589, 609
Lsp1109I GCAGC 1 cut(s) 335
MabI ACCWGGT 1 cut(s) 634
MaeIII GTNAC 1 cut(s) 10
MalI GATC 4 cut(s) 265, 337, 591, 611
MboI GATC 4 cut(s) 263, 335, 589, 609
MflI RGATCY 2 cut(s) 263, 335
MlsI TGGCCA 2 cut(s) 559, 693
MluCI AATT 2 cut(s) 152, 313
MluNI TGGCCA 2 cut(s) 559, 693
MlyI GAGTC 2 cut(s) 39, 432
MmeI TCCRAC 1 cut(s) 620
MnlI CCTC 7 cut(s) 30, 348, 351, 393, 406, 468, 559
Mox20I TGGCCA 2 cut(s) 559, 693
MroXI GAANNNNTTC 1 cut(s) 438
MscI TGGCCA 2 cut(s) 559, 693
MseI TTAA 5 cut(s) 78, 150, 288, 665, 709
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 2 cut(s) 559, 693
MspR9I CCNGG 3 cut(s) 42, 636, 681
MvaI CCWGG 3 cut(s) 42, 636, 681
NdeII GATC 4 cut(s) 263, 335, 589, 609
NlaIII CATG 6 cut(s) 160, 172, 212, 323, 590, 656
NlaIV GGNNCC 1 cut(s) 570
NmuCI GTSAC 1 cut(s) 10
PagI TCATGA 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 465, 502
PkrI GCNGC 1 cut(s) 325
PleI GAGTC 2 cut(s) 39, 431
PpsI GAGTC 2 cut(s) 39, 431
Psp6I CCWGG 3 cut(s) 40, 634, 679
PspGI CCWGG 3 cut(s) 40, 634, 679
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 2 cut(s) 17, 415
PstI CTGCAG 2 cut(s) 70, 93
PsuI RGATCY 2 cut(s) 263, 335
RseI CAYNNNNRTG 1 cut(s) 585
SaqAI TTAA 5 cut(s) 78, 150, 288, 665, 709
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 4 cut(s) 263, 335, 589, 609
Sau96I GGNCC 2 cut(s) 17, 415
SchI GAGTC 2 cut(s) 39, 432
ScrFI CCNGG 3 cut(s) 42, 636, 681
SexAI ACCWGGT 1 cut(s) 634
SfcI CTRYAG 2 cut(s) 66, 89
SinI GGWCC 2 cut(s) 17, 415
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 2 cut(s) 152, 313
SsiI CCGC 1 cut(s) 242
StyD4I CCNGG 3 cut(s) 40, 634, 679
TaaI ACNGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 411
TasI AATT 2 cut(s) 152, 313
TfiI GAWTC 2 cut(s) 465, 502
Tru1I TTAA 5 cut(s) 78, 150, 288, 665, 709
Tru9I TTAA 5 cut(s) 78, 150, 288, 665, 709
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 145, 356, 569, 645
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 93
VpaK11BI GGWCC 2 cut(s) 17, 415
XapI RAATTY 1 cut(s) 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.