MD05G1232500.v1.1
ERF Family

2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001917
Physical Location & Seq
Forward (+)
36547924 .. 36551008
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232500.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGTTAATAACAATGACCCTCGTGACTGGTGCAAGACCTCTGGTTGTAATGCAGATCCTCAATTGTGGCTAGAAACCTATGATTGCAGACCTGGACTCACTCCTTTAGAGCCCGACAACACAATGGTATTTCCTCCAGCAGCTTTACATGATATATTTTCACTGCTTGCAAAGGCAGCTCGAGATATATTGGACGCTATCAGCTTCTATTTGAACTTGAGGAGCTCACCATTTACTAAAGGGACACAACACCATAGTTTTACGACCCAAGAGGATGGCCAGCTGGTTATGTTTTCTGACCATGAGCATCAAGTTGACAAAAGCTTGATATCTCTTGTCAAGTCAGACAAGGTTGGTCTACACATAAAGGACTTTCAGGGTCGTTGGGTTCTTGTGGACGAAGATCTTGGTCCTCAAGAAGCTATTGTTTACCCTGGACTCGCACTCTATCAGGCAACTGCAGGATATGTTAACCCTGCACTGCAGAGAACGGAGACTGCTAATACACAGAGCAACTTGTTTGGGCGATGTATTTTGGCTTTTAAATTCATGCCTAAATCCATGACCAGTCTTAGTTGTTCAGAGATGAGAGCTGCTGGTAATGGGGTTGAATCTCAGTTCGAGCTTCCAGCAGCGGTTGATGACTATATGCCGATATCTCACCCCACCTGTTCGCGTCAGGAATTTCCATCGGGGATGTTTCCTGGCCGACGAGCACACAGCTCCCCTGGAGGTTGGCGCCGGTTGAGGGCTGATGTCGGGCTTCTGCTTCGCTGTCGGGCGTTGTCGAGCAAGTCTAGTCGGGCAAGTCTCGTCGGGCAAGACTCTTCAGGCAAGGCTCGTCGGGCAAGGCTGAAAGAGAATGACAGCGTTAACTTTGAGATGTGCCTTTGTGGGGCCTTAGGTGTAGGCCTTGAGGCTCACAATCAAGGATCTATGAAGCCTTTGTTGAGGAGGAGAAAGAGTGAAAGAACCAAACCTCTGCCACCTTCCAAGAGATTGTGGGTCGAGGCCCAGAGAGTTCTGAAGGAAAGGGTTCAAGACATTGCAGACAAGAAGGGAATCAAGCTGAGGTTGTGCAACCTGAAGGAGTGTGAGAATCACATTCAGACACTTGATAGCCCCTTTGCCAATATAAGAATGAAGAATGGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

386

Amino Acids

42.97

Weight (kDa)

8.91

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 738
AccI GTMKAC 1 cut(s) 358
AccII CGCG 1 cut(s) 676
AciI CCGC 1 cut(s) 635
AclWI GGATC 2 cut(s) 50, 940
AcoI YGGCCR 2 cut(s) 277, 706
AcsI RAATTY 2 cut(s) 545, 683
AcuI CTGAAG 3 cut(s) 813, 1046, 1106
AcyI GRCGYC 1 cut(s) 739
AfiI CCNNNNNNNGG 2 cut(s) 748, 895
AgsI TTSAA 3 cut(s) 214, 611, 1040
AjnI CCWGG 4 cut(s) 91, 433, 703, 727
Alw21I GWGCWC 2 cut(s) 227, 718
Alw26I GTCTC 2 cut(s) 488, 815
AlwI GGATC 2 cut(s) 50, 940
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 5 cut(s) 277, 706, 897, 910, 1011
ApeKI GCWGC 4 cut(s) 140, 176, 593, 632
ApoI RAATTY 2 cut(s) 545, 683
Asp700I GAANNNNTTC 1 cut(s) 1035
AspLEI GCGC 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 410, 897, 1012
AsuHPI GGTGA 2 cut(s) 219, 653
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 901
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 738
BanII GRGCYC 2 cut(s) 114, 227
BauI CACGAG 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 227, 718
BbvCI CCTCAGC 1 cut(s) 1070
BbvI GCAGC 4 cut(s) 152, 188, 580, 644
BccI CCATC 2 cut(s) 269, 697
BciT130I CCWGG 4 cut(s) 93, 435, 705, 729
BcoDI GTCTC 2 cut(s) 488, 815
BfaI CTAG 2 cut(s) 71, 798
BfmI CTRYAG 2 cut(s) 459, 482
BfoI RGCGCY 1 cut(s) 742
BglII AGATCT 1 cut(s) 403
BisI GCNGC 4 cut(s) 141, 177, 594, 633
BlsI GCNGC 4 cut(s) 142, 178, 595, 634
Bme1390I CCNGG 4 cut(s) 93, 435, 705, 729
Bme18I GGWCC 1 cut(s) 410
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 3 cut(s) 410, 897, 1012
BmiI GGNNCC 2 cut(s) 740, 898
BmrFI CCNGG 4 cut(s) 93, 435, 705, 729
BmsI GCATC 1 cut(s) 316
BpmI CTGGAG 2 cut(s) 120, 750
Bpu10I CCTNAGC 1 cut(s) 1070
BpuEI CTTGAG 3 cut(s) 238, 399, 935
BsaHI GRCGYC 1 cut(s) 739
BsaJI CCNNGG 2 cut(s) 433, 727
BsaXI ACNNNNNCTCC 2 cut(s) 949, 979
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 895
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 2 cut(s) 32, 567
Bse21I CCTNAGG 1 cut(s) 901
Bse3DI GCAATG 1 cut(s) 1044
BseBI CCWGG 4 cut(s) 93, 435, 705, 729
BseDI CCNNGG 2 cut(s) 433, 727
BseGI GGATG 2 cut(s) 280, 702
BseLI CCNNNNNNNGG 2 cut(s) 748, 895
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 2 cut(s) 629, 1061
BseNI ACTGG 2 cut(s) 32, 567
BseRI GAGGAG 3 cut(s) 235, 967, 970
BseXI GCAGC 4 cut(s) 152, 188, 580, 644
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 676
BshFI GGCC 5 cut(s) 279, 708, 899, 912, 1013
BshNI GGYRCC 1 cut(s) 738
BsiHKAI GWGCWC 2 cut(s) 227, 718
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 1 cut(s) 742
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 748, 895
BsmAI GTCTC 2 cut(s) 488, 815
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 5 cut(s) 279, 708, 899, 912, 1013
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 3 cut(s) 114, 227, 718
Bsp143I GATC 3 cut(s) 55, 403, 932
BspACI CCGC 1 cut(s) 635
BspANI GGCC 5 cut(s) 279, 708, 899, 912, 1013
BspCNI CTCAG 2 cut(s) 628, 1062
BspFNI CGCG 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 740, 898
BspMAI CTGCAG 2 cut(s) 463, 486
BspPI GGATC 2 cut(s) 50, 940
BspT107I GGYRCC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 2 cut(s) 32, 567
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 2 cut(s) 433, 727
BssMI GATC 3 cut(s) 55, 403, 932
BssNI GRCGYC 1 cut(s) 739
BssSI CACGAG 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 21
Bst2UI CCWGG 4 cut(s) 93, 435, 705, 729
Bst6I CTCTTC 1 cut(s) 832
BstACI GRCGYC 1 cut(s) 739
BstC8I GCNNGC 2 cut(s) 168, 281
BstDEI CTNAG 4 cut(s) 572, 615, 901, 1070
BstF5I GGATG 2 cut(s) 280, 702
BstFNI CGCG 1 cut(s) 676
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 1 cut(s) 741
BstKTI GATC 3 cut(s) 58, 406, 935
BstMAI GTCTC 2 cut(s) 488, 815
BstMBI GATC 3 cut(s) 55, 403, 932
BstMWI GCNNNNNNNGC 2 cut(s) 176, 845
BstNI CCWGG 4 cut(s) 93, 435, 705, 729
BstSCI CCNGG 4 cut(s) 91, 433, 703, 727
BstSFI CTRYAG 2 cut(s) 459, 482
BstUI CGCG 1 cut(s) 676
BstV1I GCAGC 4 cut(s) 152, 188, 580, 644
BstX2I RGATCY 3 cut(s) 55, 403, 932
BstXI CCANNNNNNTGG 1 cut(s) 275
BstYI RGATCY 3 cut(s) 55, 403, 932
Bsu36I CCTNAGG 1 cut(s) 901
BsuRI GGCC 5 cut(s) 279, 708, 899, 912, 1013
BtgZI GCGATG 1 cut(s) 541
BtsCI GGATG 2 cut(s) 280, 702
BtsI GCAGTG 2 cut(s) 161, 479
BtsIMutI CAGTG 2 cut(s) 161, 479
Cac8I GCNNGC 2 cut(s) 168, 281
CfoI GCGC 1 cut(s) 741
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 410, 897, 1012
CseI GACGC 2 cut(s) 203, 665
CviAII CATG 4 cut(s) 149, 302, 550, 562
DdeI CTNAG 4 cut(s) 572, 615, 901, 1070
DinI GGCGCC 1 cut(s) 740
DpnI GATC 3 cut(s) 57, 405, 934
DpnII GATC 3 cut(s) 55, 403, 932
DraI TTTAAA 1 cut(s) 544
EaeI YGGCCR 2 cut(s) 277, 706
Eam1104I CTCTTC 1 cut(s) 832
EarI CTCTTC 1 cut(s) 832
Ecl136II GAGCTC 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 912
Eco24I GRGCYC 2 cut(s) 114, 227
Eco32I GATATC 2 cut(s) 330, 657
Eco47I GGWCC 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 3 cut(s) 813, 1046, 1106
Eco81I CCTNAGG 1 cut(s) 901
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 897
EcoRII CCWGG 4 cut(s) 91, 433, 703, 727
EcoRV GATATC 2 cut(s) 330, 657
EcoT38I GRGCYC 2 cut(s) 114, 227
EgeI GGCGCC 1 cut(s) 740
EheI GGCGCC 1 cut(s) 740
FaeI CATG 4 cut(s) 152, 305, 553, 565
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 148, 301, 549, 561
FblI GTMKAC 1 cut(s) 358
Fnu4HI GCNGC 4 cut(s) 141, 177, 594, 633
FokI GGATG 2 cut(s) 287, 709
FriOI GRGCYC 2 cut(s) 114, 227
Fsp4HI GCNGC 4 cut(s) 141, 177, 594, 633
FspBI CTAG 2 cut(s) 71, 798
GlaI GCGC 1 cut(s) 740
GluI GCNGC 4 cut(s) 141, 177, 594, 633
GsuI CTGGAG 2 cut(s) 120, 750
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 5 cut(s) 279, 708, 899, 912, 1013
HapII CCGG 1 cut(s) 742
HgaI GACGC 2 cut(s) 203, 665
HhaI GCGC 1 cut(s) 741
Hin1I GRCGYC 1 cut(s) 739
Hin1II CATG 4 cut(s) 152, 305, 553, 565
Hin6I GCGC 1 cut(s) 739
HinP1I GCGC 1 cut(s) 739
HincII GTYRAC 3 cut(s) 316, 472, 874
HindII GTYRAC 3 cut(s) 316, 472, 874
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 6 cut(s) 96, 438, 611, 824, 1062, 1099
HpaI GTTAAC 2 cut(s) 472, 874
HpaII CCGG 1 cut(s) 742
HphI GGTGA 2 cut(s) 219, 653
Hpy166II GTNNAC 6 cut(s) 316, 359, 397, 430, 472, 874
Hpy188I TCNGA 5 cut(s) 298, 346, 583, 1026, 1110
Hpy188III TCNNGA 5 cut(s) 23, 182, 416, 680, 1040
Hpy8I GTNNAC 6 cut(s) 316, 359, 397, 430, 472, 874
Hpy99I CGWCG 3 cut(s) 714, 818, 846
HpyAV CCTTC 4 cut(s) 999, 1021, 1051, 1081
HpyCH4V TGCA 9 cut(s) 33, 53, 87, 170, 461, 479, 484, 1049, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 845
HpyF3I CTNAG 4 cut(s) 572, 615, 901, 1070
Hsp92I GRCGYC 1 cut(s) 739
Hsp92II CATG 4 cut(s) 152, 305, 553, 565
HspAI GCGC 1 cut(s) 739
KasI GGCGCC 1 cut(s) 738
KspAI GTTAAC 2 cut(s) 472, 874
Kzo9I GATC 3 cut(s) 55, 403, 932
LmnI GCTCC 2 cut(s) 222, 728
Lsp1109I GCAGC 4 cut(s) 152, 188, 580, 644
LweI GCATC 1 cut(s) 316
MaeI CTAG 2 cut(s) 71, 798
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 57, 405, 934
MboI GATC 3 cut(s) 55, 403, 932
MboII GAAGA 3 cut(s) 413, 819, 1156
MfeI CAATTG 1 cut(s) 62
MflI RGATCY 3 cut(s) 55, 403, 932
MhlI GDGCHC 3 cut(s) 114, 227, 718
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 62, 545, 683
MluNI TGGCCA 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 739
MlyI GAGTC 3 cut(s) 90, 432, 818
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 1035
MscI TGGCCA 1 cut(s) 279
MseI TTAA 4 cut(s) 6, 471, 543, 873
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 283, 635
MspI CCGG 1 cut(s) 742
MspR9I CCNGG 4 cut(s) 93, 435, 705, 729
MunI CAATTG 1 cut(s) 62
MvaI CCWGG 4 cut(s) 93, 435, 705, 729
MvnI CGCG 1 cut(s) 676
MwoI GCNNNNNNNGC 2 cut(s) 176, 845
NarI GGCGCC 1 cut(s) 739
NdeII GATC 3 cut(s) 55, 403, 932
NlaIII CATG 4 cut(s) 152, 305, 553, 565
NlaIV GGNNCC 2 cut(s) 740, 898
NmuCI GTSAC 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 180
PceI AGGCCT 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 3 cut(s) 611, 1062, 1099
PkrI GCNGC 4 cut(s) 142, 178, 595, 634
PleI GAGTC 3 cut(s) 90, 432, 818
PluTI GGCGCC 1 cut(s) 742
PpsI GAGTC 3 cut(s) 90, 432, 818
Psp124BI GAGCTC 1 cut(s) 227
Psp6I CCWGG 4 cut(s) 91, 433, 703, 727
PspGI CCWGG 4 cut(s) 91, 433, 703, 727
PspN4I GGNNCC 2 cut(s) 740, 898
PspPI GGNCC 3 cut(s) 410, 897, 1012
PstI CTGCAG 2 cut(s) 463, 486
PsuI RGATCY 3 cut(s) 55, 403, 932
PvuII CAGCTG 1 cut(s) 283
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 4 cut(s) 6, 471, 543, 873
SatI GCNGC 4 cut(s) 141, 177, 594, 633
Sau3AI GATC 3 cut(s) 55, 403, 932
Sau96I GGNCC 3 cut(s) 410, 897, 1012
SchI GAGTC 3 cut(s) 90, 432, 818
ScrFI CCNGG 4 cut(s) 93, 435, 705, 729
SduI GDGCHC 3 cut(s) 114, 227, 718
SfaNI GCATC 1 cut(s) 316
SfcI CTRYAG 2 cut(s) 459, 482
SfoI GGCGCC 1 cut(s) 740
Sfr274I CTCGAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 180
SmlI CTYRAG 4 cut(s) 180, 217, 414, 914
SmoI CTYRAG 4 cut(s) 180, 217, 414, 914
Sse9I AATT 3 cut(s) 62, 545, 683
SseBI AGGCCT 1 cut(s) 912
SsiI CCGC 1 cut(s) 635
SspDI GGCGCC 1 cut(s) 738
SspMI CTAG 2 cut(s) 71, 798
SstI GAGCTC 1 cut(s) 227
StuI AGGCCT 1 cut(s) 912
StyD4I CCNGG 4 cut(s) 91, 433, 703, 727
TaqI TCGA 4 cut(s) 181, 621, 788, 1008
TasI AATT 3 cut(s) 62, 545, 683
TfiI GAWTC 3 cut(s) 611, 1062, 1099
Tru1I TTAA 4 cut(s) 6, 471, 543, 873
Tru9I TTAA 4 cut(s) 6, 471, 543, 873
TscAI CASTG 2 cut(s) 168, 486
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 4 cut(s) 140, 176, 593, 632
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 3 cut(s) 538, 953, 1157
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 2 cut(s) 168, 486
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 2 cut(s) 545, 683
XhoI CTCGAG 1 cut(s) 180
XmiI GTMKAC 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 2 cut(s) 71, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.