Prupe.4G139600_v2.0.a1
ERF Family

2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001917
Physical Location & Seq
Forward (+)
7795253 .. 7800153
4901 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G139600.1

Sequence Viewer

Length: 1431 bp
ATGGCAGGTAATGTCCTGCCATCTCTGGGTCGTGTGAAGCTAACTGATCTCGCTCCTGCTGAAGGTCTTCCATCAGATTCATACAAACTATCTGTTTCAACTTTGTCGCAGTCACTAGCTCAATATTCTGCTGCCATCATTCAATTTCCAGTAAGTGATGGGGCTCTTTTGAGATCTGGTTTAGATTCGGCTCGCCTCTACTTCCACCAAAAAGCATCATACCCAGCTGCAGATATGGTTAATACCAATGACCCTCGTGACTGGTGCAAGACTTCCGGTTATTATGCAGATCCTCAATTGTGGCTAGAAACCTATGATTACAGACCTGGACTCACTCCCTTAGAGCCCAACAACACAATGGAATTTCCTCCAGCAGGTTTACCAGATATATTTTCACTGCTTGGAAAGGCAGCTCGGGATATATTGGATGCTATCAGCTTCTATTTGAACTTGCGGAGCTCACCATTTACTAAAGTACTTGATAATGTTCCCTTAAGAAATGGGGAAATATCTTCTTCAGTACTGTCTGTCTGCTGTCATACAAGACCATCATTTCAGGGAGCACAACACCATAATTTTACGACTCAAGAGGATGGCCAGTTGGTTATGTTTTCTGACCATGAGCATCAAGTTGACAAAAGCTTGATATCTCTTGTCAAGTCAGACAAGGCAGGTCTGCACATAAAGGACTTTCAGGGTCGTTGGGTTCTTGTGGATGAAGATCTTGGTCCCCAAGAAGCTATTGTTTACCCTGGCCTTGCACTCTATCAGGCAACTGCTGGATATGTCAACCCTGCACTGCAAAGAACAGAGACTACTAATATGCAGAGCAACTTGTTTGGCCGATGTTCTTTGGCTTTTAAACTCATGCCTAAATCCATGACCAGTCTCAGTTGTTCAGAGATGAGAGATGCTGGTCATGGAGTTGAAGCTCAGTTCCAGCTGCCAGTTCCGGTCGATGACTTTATGCAGAGATCTCACCCAACCGATCAACTTTTCAACAGACAGAGTTTTCAAAGTTTCAATTTCCATGCAACCCAAGAAGGATCTATGAAGCCTTTGTTAAGGAGGAGGAAGAATGATTCAAGATCAAAACCTCTGCCACCTTCCAAGAGATTAAGGCTTGAGGCCCAGAGAGTTCTGAAGGAAAGGGTTCAGGACATTGCAGACAAGAAGGGAATCAAGCTGAGGTTCTGCAACCTGAAGGAGTGTGAGAATCATATTCTCACACTTGATAGCCCATGTGCCAATATAAGAATGGAGATTGGGTGGCCACATGGAGTGCCATTTGTTCATCCCCATGATCTACCCAATAAGGCAAAGATTGGTTTCCTTGAAGCATATGAACCTGGTTGGACAGCTACTCATGAAATGGAGTTAAGTCTAACTGAACCAGGACAGGCCAGTCAACACTCGGTTAATTGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

53.16

Weight (kDa)

6.47

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1404
Acc36I ACCTGC 2 cut(s) 365, 662
AciI CCGC 1 cut(s) 454
AclWI GGATC 2 cut(s) 284, 1054
AcoI YGGCCR 3 cut(s) 595, 841, 1271
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 4 cut(s) 81, 501, 1163, 1223
AfaI GTAC 2 cut(s) 477, 522
AfiI CCNNNNNNNGG 3 cut(s) 26, 62, 374
AflII CTTAAG 1 cut(s) 493
AgsI TTSAA 9 cut(s) 99, 143, 448, 929, 1000, 1016, 1024, 1086, 1337
AjnI CCWGG 4 cut(s) 325, 751, 1348, 1393
Alw21I GWGCWC 2 cut(s) 461, 565
Alw26I GTCTC 2 cut(s) 806, 893
AlwI GGATC 2 cut(s) 284, 1054
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 6 cut(s) 595, 754, 841, 1128, 1271, 1401
ApeKI GCWGC 4 cut(s) 131, 227, 410, 943
ApoI RAATTY 1 cut(s) 362
Asp700I GAANNNNTTC 2 cut(s) 66, 1152
AspS9I GGNCC 2 cut(s) 728, 1129
AsuHPI GGTGA 2 cut(s) 453, 971
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 1 cut(s) 728
BalI TGGCCA 2 cut(s) 597, 1273
BanII GRGCYC 3 cut(s) 166, 348, 461
BauI CACGAG 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 461, 565
BbvCI CCTCAGC 1 cut(s) 1187
BbvI GCAGC 4 cut(s) 118, 214, 422, 930
BccI CCATC 6 cut(s) 28, 79, 143, 152, 556, 587
BciT130I CCWGG 4 cut(s) 327, 753, 1350, 1395
BcoDI GTCTC 2 cut(s) 806, 893
BfaI CTAG 2 cut(s) 116, 305
BfmI CTRYAG 1 cut(s) 228
BfrI CTTAAG 1 cut(s) 493
BfuAI ACCTGC 2 cut(s) 365, 662
BglII AGATCT 3 cut(s) 173, 721, 974
BisI GCNGC 4 cut(s) 132, 228, 411, 944
BlsI GCNGC 4 cut(s) 133, 229, 412, 945
BmcAI AGTACT 2 cut(s) 477, 522
Bme1390I CCNGG 4 cut(s) 327, 753, 1350, 1395
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 2 cut(s) 728, 1129
BmiI GGNNCC 1 cut(s) 730
BmrFI CCNGG 4 cut(s) 327, 753, 1350, 1395
BmsI GCATC 4 cut(s) 224, 418, 634, 901
BpiI GAAGAC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 1187
BpuEI CTTGAG 2 cut(s) 570, 1145
BsaBI GATNNNNATC 1 cut(s) 720
BsaJI CCNNGG 1 cut(s) 751
BsaWI WCCGGW 2 cut(s) 275, 952
BsaXI ACNNNNNCTCC 2 cut(s) 1253, 1283
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 62, 374
Bse1I ACTGG 6 cut(s) 149, 266, 598, 885, 947, 1404
Bse3DI GCAATG 1 cut(s) 1161
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 4 cut(s) 327, 753, 1350, 1395
BseDI CCNNGG 1 cut(s) 751
BseGI GGATG 4 cut(s) 433, 598, 721, 1294
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 3 cut(s) 26, 62, 374
BseMI GCAATG 1 cut(s) 1161
BseMII CTCAG 3 cut(s) 904, 947, 1178
BseNI ACTGG 6 cut(s) 149, 266, 598, 885, 947, 1404
BseRI GAGGAG 1 cut(s) 1084
BseXI GCAGC 4 cut(s) 118, 214, 422, 930
BseYI CCCAGC 1 cut(s) 223
BsgI GTGCAG 2 cut(s) 662, 780
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 6 cut(s) 597, 756, 843, 1130, 1273, 1403
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 2 cut(s) 461, 565
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 2 cut(s) 276, 953
BslFI GGGAC 1 cut(s) 714
BslI CCNNNNNNNGG 3 cut(s) 26, 62, 374
BsmAI GTCTC 2 cut(s) 806, 893
BsmFI GGGAC 1 cut(s) 714
BsnI GGCC 6 cut(s) 597, 756, 843, 1130, 1273, 1403
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 4 cut(s) 166, 348, 461, 565
Bsp143I GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
BspACI CCGC 1 cut(s) 454
BspANI GGCC 6 cut(s) 597, 756, 843, 1130, 1273, 1403
BspCNI CTCAG 3 cut(s) 903, 946, 1179
BspHI TCATGA 1 cut(s) 1366
BspLI GGNNCC 1 cut(s) 730
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 2 cut(s) 365, 662
BspPI GGATC 2 cut(s) 284, 1054
BspTI CTTAAG 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 1161
BsrI ACTGG 6 cut(s) 149, 266, 598, 885, 947, 1404
BssECI CCNNGG 1 cut(s) 751
BssMI GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
BssSI CACGAG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 255
Bst2UI CCWGG 4 cut(s) 327, 753, 1350, 1395
Bst4CI ACNGT 1 cut(s) 525
BstAFI CTTAAG 1 cut(s) 493
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 4 cut(s) 340, 890, 933, 1187
BstENI CCTNNNNNAGG 2 cut(s) 60, 372
BstF5I GGATG 4 cut(s) 433, 598, 721, 1294
BstKTI GATC 9 cut(s) 49, 176, 292, 724, 977, 991, 1049, 1091, 1306
BstMAI GTCTC 2 cut(s) 806, 893
BstMBI GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
BstMCI CGRYCG 1 cut(s) 957
BstNI CCWGG 4 cut(s) 327, 753, 1350, 1395
BstSCI CCNGG 4 cut(s) 325, 751, 1348, 1393
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 4 cut(s) 118, 214, 422, 930
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 5 cut(s) 173, 289, 721, 974, 1046
BstYI RGATCY 5 cut(s) 173, 289, 721, 974, 1046
BsuRI GGCC 6 cut(s) 597, 756, 843, 1130, 1273, 1403
BtsCI GGATG 4 cut(s) 433, 598, 721, 1294
BtsI GCAGTG 2 cut(s) 395, 797
BtsIMutI CAGTG 2 cut(s) 395, 797
BveI ACCTGC 2 cut(s) 365, 662
Cac8I GCNNGC 1 cut(s) 193
CciI TCATGA 1 cut(s) 1366
Cfr13I GGNCC 2 cut(s) 728, 1129
CsiI ACCWGGT 1 cut(s) 1348
Csp6I GTAC 2 cut(s) 476, 521
CviAII CATG 9 cut(s) 620, 868, 880, 920, 1031, 1242, 1277, 1301, 1367
CviQI GTAC 2 cut(s) 476, 521
DdeI CTNAG 4 cut(s) 340, 890, 933, 1187
DpnI GATC 9 cut(s) 48, 175, 291, 723, 976, 990, 1048, 1090, 1305
DpnII GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
DraI TTTAAA 1 cut(s) 862
DrdI GACNNNNNNGTC 1 cut(s) 1404
DseDI GACNNNNNNGTC 1 cut(s) 1404
EaeI YGGCCR 3 cut(s) 595, 841, 1271
Ecl136II GAGCTC 1 cut(s) 459
Eco24I GRGCYC 3 cut(s) 166, 348, 461
Eco32I GATATC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 728
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 4 cut(s) 81, 501, 1163, 1223
Eco88I CYCGRG 1 cut(s) 414
EcoICRI GAGCTC 1 cut(s) 459
EcoNI CCTNNNNNAGG 2 cut(s) 60, 372
EcoRII CCWGG 4 cut(s) 325, 751, 1348, 1393
EcoRV GATATC 1 cut(s) 648
EcoT38I GRGCYC 3 cut(s) 166, 348, 461
FaeI CATG 9 cut(s) 623, 871, 883, 923, 1034, 1245, 1280, 1304, 1370
FaqI GGGAC 1 cut(s) 714
FatI CATG 9 cut(s) 619, 867, 879, 919, 1030, 1241, 1276, 1300, 1366
FauNDI CATATG 1 cut(s) 1342
Fnu4HI GCNGC 4 cut(s) 132, 228, 411, 944
FokI GGATG 4 cut(s) 440, 605, 728, 1281
FriOI GRGCYC 3 cut(s) 166, 348, 461
Fsp4HI GCNGC 4 cut(s) 132, 228, 411, 944
FspBI CTAG 2 cut(s) 116, 305
GluI GCNGC 4 cut(s) 132, 228, 411, 944
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 6 cut(s) 597, 756, 843, 1130, 1273, 1403
HapII CCGG 2 cut(s) 276, 953
Hin1II CATG 9 cut(s) 623, 871, 883, 923, 1034, 1245, 1280, 1304, 1370
HincII GTYRAC 3 cut(s) 634, 790, 1409
HindII GTYRAC 3 cut(s) 634, 790, 1409
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 7 cut(s) 77, 185, 330, 583, 1082, 1179, 1216
HpaII CCGG 2 cut(s) 276, 953
HphI GGTGA 2 cut(s) 453, 971
Hpy166II GTNNAC 5 cut(s) 380, 634, 748, 790, 1409
Hpy188I TCNGA 5 cut(s) 76, 616, 664, 901, 1143
Hpy188III TCNNGA 6 cut(s) 257, 416, 587, 1086, 1157, 1367
Hpy8I GTNNAC 5 cut(s) 380, 634, 748, 790, 1409
HpyAV CCTTC 6 cut(s) 56, 1037, 1116, 1138, 1168, 1198
HpyCH4III ACNGT 1 cut(s) 525
HpyF3I CTNAG 4 cut(s) 340, 890, 933, 1187
Hsp92II CATG 9 cut(s) 623, 871, 883, 923, 1034, 1245, 1280, 1304, 1370
Kzo9I GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
LmnI GCTCC 3 cut(s) 58, 456, 560
Lsp1109I GCAGC 4 cut(s) 118, 214, 422, 930
LweI GCATC 4 cut(s) 224, 418, 634, 901
MabI ACCWGGT 1 cut(s) 1348
MaeI CTAG 2 cut(s) 116, 305
MaeIII GTNAC 2 cut(s) 111, 257
MalI GATC 9 cut(s) 48, 175, 291, 723, 976, 990, 1048, 1090, 1305
MboI GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
MboII GAAGA 5 cut(s) 59, 504, 507, 731, 1087
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 5 cut(s) 173, 289, 721, 974, 1046
MhlI GDGCHC 4 cut(s) 166, 348, 461, 565
MlsI TGGCCA 2 cut(s) 597, 1273
MluCI AATT 7 cut(s) 143, 296, 362, 574, 1024, 1420, 1426
MluNI TGGCCA 2 cut(s) 597, 1273
MlyI GAGTC 2 cut(s) 324, 577
MmeI TCCRAC 1 cut(s) 1334
Mox20I TGGCCA 2 cut(s) 597, 1273
MroXI GAANNNNTTC 2 cut(s) 66, 1152
MscI TGGCCA 2 cut(s) 597, 1273
MseI TTAA 7 cut(s) 240, 494, 861, 1064, 1118, 1379, 1419
MslI CAYNNNNRTG 1 cut(s) 1299
Msp20I TGGCCA 2 cut(s) 597, 1273
MspA1I CMGCKG 2 cut(s) 227, 943
MspCI CTTAAG 1 cut(s) 493
MspI CCGG 2 cut(s) 276, 953
MspR9I CCNGG 4 cut(s) 327, 753, 1350, 1395
MunI CAATTG 1 cut(s) 296
MvaI CCWGG 4 cut(s) 327, 753, 1350, 1395
NdeI CATATG 1 cut(s) 1342
NdeII GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
NlaIII CATG 9 cut(s) 623, 871, 883, 923, 1034, 1245, 1280, 1304, 1370
NlaIV GGNNCC 1 cut(s) 730
NmuCI GTSAC 2 cut(s) 111, 257
PagI TCATGA 1 cut(s) 1366
PdmI GAANNNNTTC 2 cut(s) 66, 1152
PfeI GAWTC 5 cut(s) 77, 185, 1082, 1179, 1216
PkrI GCNGC 4 cut(s) 133, 229, 412, 945
PleI GAGTC 2 cut(s) 324, 577
PpsI GAGTC 2 cut(s) 324, 577
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 4 cut(s) 325, 751, 1348, 1393
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 4 cut(s) 325, 751, 1348, 1393
PspN4I GGNNCC 1 cut(s) 730
PspPI GGNCC 2 cut(s) 728, 1129
PsrI GAACNNNNNNTAC 2 cut(s) 799, 831
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 5 cut(s) 173, 289, 721, 974, 1046
PvuII CAGCTG 2 cut(s) 227, 943
RsaI GTAC 2 cut(s) 477, 522
RsaNI GTAC 2 cut(s) 476, 521
RseI CAYNNNNRTG 1 cut(s) 1299
SacI GAGCTC 1 cut(s) 461
SaqAI TTAA 7 cut(s) 240, 494, 861, 1064, 1118, 1379, 1419
SatI GCNGC 4 cut(s) 132, 228, 411, 944
Sau3AI GATC 9 cut(s) 46, 173, 289, 721, 974, 988, 1046, 1088, 1303
Sau96I GGNCC 2 cut(s) 728, 1129
ScaI AGTACT 2 cut(s) 477, 522
SchI GAGTC 2 cut(s) 324, 577
ScrFI CCNGG 4 cut(s) 327, 753, 1350, 1395
SduI GDGCHC 4 cut(s) 166, 348, 461, 565
SexAI ACCWGGT 1 cut(s) 1348
SfaNI GCATC 4 cut(s) 224, 418, 634, 901
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 1299
SmlI CTYRAG 3 cut(s) 493, 585, 1124
SmoI CTYRAG 3 cut(s) 493, 585, 1124
Sse9I AATT 7 cut(s) 143, 296, 362, 574, 1024, 1420, 1426
SsiI CCGC 1 cut(s) 454
SspI AATATT 1 cut(s) 125
SspMI CTAG 2 cut(s) 116, 305
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 4 cut(s) 325, 751, 1348, 1393
TaaI ACNGT 1 cut(s) 525
TaqI TCGA 1 cut(s) 957
TasI AATT 7 cut(s) 143, 296, 362, 574, 1024, 1420, 1426
TatI WGTACW 2 cut(s) 475, 520
TfiI GAWTC 5 cut(s) 77, 185, 1082, 1179, 1216
Tru1I TTAA 7 cut(s) 240, 494, 861, 1064, 1118, 1379, 1419
Tru9I TTAA 7 cut(s) 240, 494, 861, 1064, 1118, 1379, 1419
TscAI CASTG 2 cut(s) 402, 804
TseFI GTSAC 2 cut(s) 111, 257
TseI GCWGC 4 cut(s) 131, 227, 410, 943
Tsp45I GTSAC 2 cut(s) 111, 257
TspDTI ATGAA 6 cut(s) 69, 732, 1067, 1283, 1359, 1383
TspRI CASTG 2 cut(s) 402, 804
Vha464I CTTAAG 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 728
XagI CCTNNNNNAGG 2 cut(s) 60, 372
XapI RAATTY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 2 cut(s) 355, 1255
XmnI GAANNNNTTC 2 cut(s) 66, 1152
XspI CTAG 2 cut(s) 116, 305
ZrmI AGTACT 2 cut(s) 477, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.