pycom05g20160
ERF Family

2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001917
Physical Location & Seq
Reverse (-)
23027099 .. 23027644
546 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20160.1

Sequence Viewer

Length: 546 bp
ATGACCCTCGTGACTGGTGCAAGACTTCCGGTTATAATGCCGATTGATCCTCAATTGTGGCTAGAAACCTATGATTACAGACCTGGACTCACTCCTTTAGAGCCCGACAACACAATGGAATTTCCTCCGGCAGCTTTACCAGATATATTTTCACTGCTTGCAAAGGCAGCTCGAGATATATTGGACGCTATCAGCTTCTATTTGAACTTGAGGAGCTCACCATTTACTGAAGTACTTGATAATGTTCCCTTAAGAAACCGGGAAATATCTTCTTCAGTATTATCTGTCTGTTGTCATGCAAGGCCATCATTTCAGGGGACACAACACCATAGTTTTACGACTCAAGAGGATGGCCAGTTGGTTATGTTTTCTGACCATGAACATCAAGTTGACAAAAGCTTGATATCTCTTGTCAAGTCAGACAAGGCTGGTCTACACATAAAGGACTTTCAGGGTCGTTGGGTTCTTGTGGACGAAGATCGATCCTCAAGAAGCTATTGTTTACCCTGGACTTGCACTCTATCAGGCAACTGCAGGATATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.42

Weight (kDa)

5.43

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AccI GTMKAC 1 cut(s) 433
AclWI GGATC 2 cut(s) 41, 477
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 2 cut(s) 249, 258
AfaI GTAC 1 cut(s) 234
AflII CTTAAG 1 cut(s) 250
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 2 cut(s) 82, 506
AluBI AGCT 6 cut(s) 134, 170, 195, 216, 399, 495
AluI AGCT 6 cut(s) 134, 170, 195, 216, 399, 495
Alw21I GWGCWC 1 cut(s) 218
AlwI GGATC 2 cut(s) 41, 477
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 2 cut(s) 302, 352
ApeKI GCWGC 2 cut(s) 131, 167
ApoI RAATTY 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 171
BalI TGGCCA 1 cut(s) 354
BanII GRGCYC 2 cut(s) 105, 218
BauI CACGAG 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 143, 179
BccI CCATC 2 cut(s) 313, 344
BciT130I CCWGG 2 cut(s) 84, 508
BcnI CCSGG 1 cut(s) 260
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 532
BfrI CTTAAG 1 cut(s) 250
BisI GCNGC 2 cut(s) 132, 168
BlsI GCNGC 2 cut(s) 133, 169
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 3 cut(s) 84, 260, 508
BmeT110I CYCGRG 1 cut(s) 171
BmrFI CCNGG 3 cut(s) 84, 260, 508
BpuEI CTTGAG 3 cut(s) 229, 327, 472
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 506
BsaWI WCCGGW 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 19, 355
BseBI CCWGG 2 cut(s) 84, 508
BseCI ATCGAT 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 1 cut(s) 355
BseNI ACTGG 2 cut(s) 19, 355
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 143, 179
BshFI GGCC 2 cut(s) 304, 354
BshVI ATCGAT 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 3 cut(s) 29, 128, 259
BslFI GGGAC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 2 cut(s) 304, 354
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 105, 218
Bsp143I GATC 3 cut(s) 46, 478, 482
BspANI GGCC 2 cut(s) 304, 354
BspDI ATCGAT 1 cut(s) 481
BspMAI CTGCAG 1 cut(s) 536
BspPI GGATC 2 cut(s) 41, 477
BspTI CTTAAG 1 cut(s) 250
BsrI ACTGG 2 cut(s) 19, 355
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 3 cut(s) 46, 478, 482
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 2 cut(s) 84, 508
BstAFI CTTAAG 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 159
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 3 cut(s) 49, 481, 485
BstMBI GATC 3 cut(s) 46, 478, 482
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 2 cut(s) 84, 508
BstSCI CCNGG 3 cut(s) 82, 258, 506
BstSFI CTRYAG 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 143, 179
Bsu15I ATCGAT 1 cut(s) 481
BsuRI GGCC 2 cut(s) 304, 354
BsuTUI ATCGAT 1 cut(s) 481
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 152
Cac8I GCNNGC 1 cut(s) 159
ClaI ATCGAT 1 cut(s) 481
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 233
CviAII CATG 2 cut(s) 296, 377
CviQI GTAC 1 cut(s) 233
DpnI GATC 3 cut(s) 48, 480, 484
DpnII GATC 3 cut(s) 46, 478, 482
EaeI YGGCCR 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 105, 218
Eco32I GATATC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 2 cut(s) 249, 258
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 82, 506
EcoRV GATATC 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 105, 218
FaeI CATG 2 cut(s) 299, 380
FaqI GGGAC 1 cut(s) 331
FatI CATG 2 cut(s) 295, 376
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 2 cut(s) 132, 168
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 2 cut(s) 105, 218
Fsp4HI GCNGC 2 cut(s) 132, 168
FspBI CTAG 1 cut(s) 62
GluI GCNGC 2 cut(s) 132, 168
HaeIII GGCC 2 cut(s) 304, 354
HapII CCGG 3 cut(s) 29, 128, 259
HgaI GACGC 1 cut(s) 194
Hin1II CATG 2 cut(s) 299, 380
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 2 cut(s) 87, 340
HpaII CCGG 3 cut(s) 29, 128, 259
HphI GGTGA 1 cut(s) 210
Hpy166II GTNNAC 4 cut(s) 391, 434, 472, 503
Hpy188I TCNGA 2 cut(s) 373, 421
Hpy188III TCNNGA 4 cut(s) 10, 173, 344, 489
Hpy8I GTNNAC 4 cut(s) 391, 434, 472, 503
HpyCH4V TGCA 5 cut(s) 20, 161, 299, 516, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
Hsp92II CATG 2 cut(s) 299, 380
Kzo9I GATC 3 cut(s) 46, 478, 482
LmnI GCTCC 1 cut(s) 213
Lsp1109I GCAGC 2 cut(s) 143, 179
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 10
MalI GATC 3 cut(s) 48, 480, 484
MboI GATC 3 cut(s) 46, 478, 482
MboII GAAGA 3 cut(s) 261, 264, 488
MfeI CAATTG 1 cut(s) 53
MhlI GDGCHC 2 cut(s) 105, 218
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 2 cut(s) 53, 119
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 2 cut(s) 81, 334
MnlI CCTC 6 cut(s) 17, 60, 135, 204, 340, 496
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
MseI TTAA 2 cut(s) 251, 544
Msp20I TGGCCA 1 cut(s) 354
MspCI CTTAAG 1 cut(s) 250
MspI CCGG 3 cut(s) 29, 128, 259
MspR9I CCNGG 3 cut(s) 84, 260, 508
MunI CAATTG 1 cut(s) 53
MvaI CCWGG 2 cut(s) 84, 508
MwoI GCNNNNNNNGC 1 cut(s) 167
NciI CCSGG 1 cut(s) 260
NdeII GATC 3 cut(s) 46, 478, 482
NlaIII CATG 2 cut(s) 299, 380
NmuCI GTSAC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 171
PkrI GCNGC 2 cut(s) 133, 169
PleI GAGTC 2 cut(s) 81, 334
PpsI GAGTC 2 cut(s) 81, 334
PsiI TTATAA 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 82, 506
PspGI CCWGG 2 cut(s) 82, 506
PstI CTGCAG 1 cut(s) 536
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SacI GAGCTC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 251, 544
SatI GCNGC 2 cut(s) 132, 168
Sau3AI GATC 3 cut(s) 46, 478, 482
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 81, 334
ScrFI CCNGG 3 cut(s) 84, 260, 508
SduI GDGCHC 2 cut(s) 105, 218
SetI ASST 8 cut(s) 71, 85, 136, 172, 197, 218, 401, 497
SfcI CTRYAG 1 cut(s) 532
Sfr274I CTCGAG 1 cut(s) 171
SlaI CTCGAG 1 cut(s) 171
SmlI CTYRAG 5 cut(s) 171, 208, 250, 342, 487
SmoI CTYRAG 5 cut(s) 171, 208, 250, 342, 487
Sse9I AATT 2 cut(s) 53, 119
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 3 cut(s) 82, 258, 506
TaqI TCGA 2 cut(s) 172, 481
TasI AATT 2 cut(s) 53, 119
TatI WGTACW 1 cut(s) 232
Tru1I TTAA 2 cut(s) 251, 544
Tru9I TTAA 2 cut(s) 251, 544
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 131, 167
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 1 cut(s) 159
Vha464I CTTAAG 1 cut(s) 250
XapI RAATTY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 433
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.