Rw5G016920
ERF Family

2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001917
Physical Location & Seq
Forward (+)
21665286 .. 21669109
3824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016920.1

Sequence Viewer

Length: 1083 bp
ATGGCAGGTAGTGGCCTGCCATCTCTCGGTCGTGTGAAGCTAGCTGATCTCGTACCTTCTGAAGGCCTTCCATCAGATTCTTACAAACTATCTGTTTCAACTCTGTCACAATCATTGGCTCAGTATTCCGCTGCCATTATTCAATTTTCAGTGAGTGATGGAGCTCTTTTAAGGTCCGGTTTAGATTCTGCTCGCCTCTACTTCCATCAAAGAGCAGCATACCCAGCTCCTGACATTGTTCATAACAATGACCCTCGCGAATGGTGCAAGACTTCCGGTTACAATGCAGATCCTCAGTTGTGGCTAGAAACATATGATTACAGACCTGGAATAACTCCTTTAGAGCCCAACAACATGATGGAATTTCCTCCAGCAGGTTTGCCAGATATATTTTCAGTGCTTGGAAAGGCAGCTAGGGACATATTGGATGCGATCAGCTTCTATTTGAATTTGCGGAGCTCGCCATTTACTGAAATACTTGATAATGTTCCATTAAGAAATCGGGAAATATCTTCCTCAGTATTGTCTGTCTGCTGTCATGCAAGGCCATCATTTCAAGGAGCACAACACCATAACTTTGCAACCCAAGAGGATGGCCAGTTGGTTATGTTTTCTGACCATGAGCATCAAGTAGACAAAACCTTGATATCTGTTGTCAGGTCAGATAAGCCAGGCCTACACATAAAGGACTTTCAGGGTCGTTGGGTTCTTGTAGATGAAGATCTTGGTCCTCAAGAAGCTATTGTCTACCCTGGACTTGCACTGTATCAGGCAACTGCCGGATTTGTTAACCCTGCACTGCAAAGAACAGAGATCACTAATATACAGAACAACTTGTTTGGGCGATGTTCTTTGGCTTTTAAACTGATGCCTAAATCCATGACCAGTCTCAGTTGTTCAGAGATGAGAGCCGCTGGTCATGGGGTTGAACCTCAGTTCCAGCTTCCAGTACCAGTTGATGACTTTATGCAAAGGTCTCACCCGATCGATCAACTTTTTAACAGACAGACTGTTCAGAGTTTCAATTTCCATGCAACCCCAGATGGTCCTTGGATCGGTGAAATCCTGGTACAGGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

39.84

Weight (kDa)

5.42

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 365
AccI GTMKAC 2 cut(s) 633, 747
AccII CGCG 1 cut(s) 258
AciI CCGC 3 cut(s) 129, 454, 912
AclWI GGATC 2 cut(s) 284, 1061
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 2 cut(s) 362, 448
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 54, 951, 1071
AfiI CCNNNNNNNGG 5 cut(s) 26, 62, 374, 1055, 1072
AgsI TTSAA 6 cut(s) 99, 143, 448, 557, 929, 1024
AjnI CCWGG 4 cut(s) 325, 670, 751, 1065
AloI GAACNNNNNNTCC 2 cut(s) 921, 953
AluBI AGCT 9 cut(s) 40, 44, 164, 227, 413, 438, 459, 740, 943
AluI AGCT 9 cut(s) 40, 44, 164, 227, 413, 438, 459, 740, 943
Alw21I GWGCWC 3 cut(s) 166, 461, 565
Alw26I GTCTC 2 cut(s) 893, 981
AlwI GGATC 2 cut(s) 284, 1061
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 5 cut(s) 13, 64, 545, 595, 673
ApeKI GCWGC 3 cut(s) 131, 215, 410
ApoI RAATTY 2 cut(s) 362, 448
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 3 cut(s) 174, 728, 1046
AsuHPI GGTGA 2 cut(s) 971, 1070
AsuNHI GCTAGC 1 cut(s) 40
AvaII GGWCC 3 cut(s) 174, 728, 1046
BalI TGGCCA 1 cut(s) 597
BanII GRGCYC 3 cut(s) 166, 348, 461
Bbv12I GWGCWC 3 cut(s) 166, 461, 565
BbvI GCAGC 3 cut(s) 118, 227, 422
BccI CCATC 8 cut(s) 28, 79, 152, 213, 352, 556, 587, 1037
BciT130I CCWGG 4 cut(s) 327, 672, 753, 1067
BcoDI GTCTC 2 cut(s) 893, 981
BfaI CTAG 3 cut(s) 41, 305, 414
BfuAI ACCTGC 1 cut(s) 365
BglII AGATCT 1 cut(s) 721
BisI GCNGC 4 cut(s) 132, 216, 411, 912
BlsI GCNGC 4 cut(s) 133, 217, 412, 913
Bme1390I CCNGG 4 cut(s) 327, 672, 753, 1067
Bme18I GGWCC 3 cut(s) 174, 728, 1046
BmgT120I GGNCC 3 cut(s) 174, 728, 1046
BmrFI CCNGG 4 cut(s) 327, 672, 753, 1067
BmsI GCATC 3 cut(s) 418, 634, 858
BmtI GCTAGC 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 717
Bsa29I ATCGAT 1 cut(s) 987
BsaBI GATNNNNATC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 981
BsaJI CCNNGG 2 cut(s) 751, 1049
BsaWI WCCGGW 2 cut(s) 176, 275
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 62, 374, 1055, 1072
Bse1I ACTGG 4 cut(s) 598, 885, 947, 953
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 4 cut(s) 327, 672, 753, 1067
BseCI ATCGAT 1 cut(s) 987
BseDI CCNNGG 2 cut(s) 751, 1049
BseGI GGATG 2 cut(s) 433, 598
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 5 cut(s) 26, 62, 374, 1055, 1072
BseMII CTCAG 5 cut(s) 134, 308, 531, 904, 947
BseNI ACTGG 4 cut(s) 598, 885, 947, 953
BseXI GCAGC 3 cut(s) 118, 227, 422
BseYI CCCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 2 cut(s) 31, 987
BshFI GGCC 5 cut(s) 15, 66, 547, 597, 675
BshVI ATCGAT 1 cut(s) 987
BsiEI CGRYCG 2 cut(s) 31, 987
BsiHKAI GWGCWC 3 cut(s) 166, 461, 565
BsiSI CCGG 3 cut(s) 177, 276, 780
BslFI GGGAC 1 cut(s) 431
BslI CCNNNNNNNGG 5 cut(s) 26, 62, 374, 1055, 1072
BsmAI GTCTC 2 cut(s) 893, 981
BsmFI GGGAC 1 cut(s) 431
BsnI GGCC 5 cut(s) 15, 66, 547, 597, 675
Bso31I GGTCTC 1 cut(s) 981
Bsp1286I GDGCHC 4 cut(s) 166, 348, 461, 565
Bsp143I GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
Bsp68I TCGCGA 1 cut(s) 258
BspACI CCGC 3 cut(s) 129, 454, 912
BspANI GGCC 5 cut(s) 15, 66, 547, 597, 675
BspCNI CTCAG 5 cut(s) 133, 307, 530, 903, 946
BspDI ATCGAT 1 cut(s) 987
BspFNI CGCG 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 365
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 2 cut(s) 284, 1061
BspTNI GGTCTC 1 cut(s) 981
BsrI ACTGG 4 cut(s) 598, 885, 947, 953
BssECI CCNNGG 2 cut(s) 751, 1049
BssMI GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
BssT1I CCWWGG 1 cut(s) 1049
Bst2UI CCWGG 4 cut(s) 327, 672, 753, 1067
Bst4CI ACNGT 2 cut(s) 765, 1012
BstC8I GCNNGC 4 cut(s) 17, 42, 193, 461
BstDEI CTNAG 5 cut(s) 120, 294, 517, 890, 933
BstENI CCTNNNNNAGG 3 cut(s) 60, 372, 1070
BstF5I GGATG 2 cut(s) 433, 598
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 8 cut(s) 49, 292, 435, 724, 816, 987, 991, 1056
BstMAI GTCTC 2 cut(s) 893, 981
BstMBI GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
BstMCI CGRYCG 2 cut(s) 31, 987
BstMWI GCNNNNNNNGC 3 cut(s) 224, 264, 460
BstNI CCWGG 4 cut(s) 327, 672, 753, 1067
BstSCI CCNGG 4 cut(s) 325, 670, 751, 1065
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 3 cut(s) 118, 227, 422
BstX2I RGATCY 2 cut(s) 289, 721
BstXI CCANNNNNNTGG 1 cut(s) 593
BstYI RGATCY 2 cut(s) 289, 721
Bsu15I ATCGAT 1 cut(s) 987
BsuRI GGCC 5 cut(s) 15, 66, 547, 597, 675
BsuTUI ATCGAT 1 cut(s) 987
BtgZI GCGATG 1 cut(s) 859
BtsCI GGATG 2 cut(s) 433, 598
BtsI GCAGTG 1 cut(s) 797
BtsIMutI CAGTG 4 cut(s) 156, 402, 761, 797
BtuMI TCGCGA 1 cut(s) 258
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 4 cut(s) 17, 42, 193, 461
CaiI CAGNNNCTG 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 174, 728, 1046
ClaI ATCGAT 1 cut(s) 987
Csp6I GTAC 3 cut(s) 53, 950, 1070
CviAII CATG 6 cut(s) 355, 539, 620, 880, 920, 1031
CviQI GTAC 3 cut(s) 53, 950, 1070
DdeI CTNAG 5 cut(s) 120, 294, 517, 890, 933
DpnI GATC 8 cut(s) 48, 291, 434, 723, 815, 986, 990, 1055
DpnII GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
DraI TTTAAA 1 cut(s) 862
EaeI YGGCCR 1 cut(s) 595
Ecl136II GAGCTC 2 cut(s) 164, 459
Eco130I CCWWGG 1 cut(s) 1049
Eco147I AGGCCT 2 cut(s) 66, 675
Eco24I GRGCYC 3 cut(s) 166, 348, 461
Eco31I GGTCTC 1 cut(s) 981
Eco32I GATATC 1 cut(s) 648
Eco47I GGWCC 3 cut(s) 174, 728, 1046
Eco53kI GAGCTC 2 cut(s) 164, 459
Eco57I CTGAAG 1 cut(s) 81
EcoICRI GAGCTC 2 cut(s) 164, 459
EcoNI CCTNNNNNAGG 3 cut(s) 60, 372, 1070
EcoRII CCWGG 4 cut(s) 325, 670, 751, 1065
EcoRV GATATC 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 1049
EcoT38I GRGCYC 3 cut(s) 166, 348, 461
ErhI CCWWGG 1 cut(s) 1049
FaeI CATG 6 cut(s) 358, 542, 623, 883, 923, 1034
FaqI GGGAC 1 cut(s) 431
FatI CATG 6 cut(s) 354, 538, 619, 879, 919, 1030
FauNDI CATATG 1 cut(s) 313
FblI GTMKAC 2 cut(s) 633, 747
Fnu4HI GCNGC 4 cut(s) 132, 216, 411, 912
FokI GGATG 2 cut(s) 440, 605
FriOI GRGCYC 3 cut(s) 166, 348, 461
Fsp4HI GCNGC 4 cut(s) 132, 216, 411, 912
FspBI CTAG 3 cut(s) 41, 305, 414
GluI GCNGC 4 cut(s) 132, 216, 411, 912
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 5 cut(s) 15, 66, 547, 597, 675
HapII CCGG 3 cut(s) 177, 276, 780
Hin1II CATG 6 cut(s) 358, 542, 623, 883, 923, 1034
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 2 cut(s) 77, 185
HpaI GTTAAC 1 cut(s) 790
HpaII CCGG 3 cut(s) 177, 276, 780
HphI GGTGA 2 cut(s) 971, 1070
Hpy166II GTNNAC 3 cut(s) 634, 748, 790
Hpy188I TCNGA 6 cut(s) 61, 76, 616, 664, 901, 1017
Hpy188III TCNNGA 4 cut(s) 230, 257, 503, 734
Hpy8I GTNNAC 3 cut(s) 634, 748, 790
HpyAV CCTTC 3 cut(s) 56, 66, 77
HpyCH4III ACNGT 2 cut(s) 765, 1012
HpyCH4V TGCA 9 cut(s) 267, 287, 542, 581, 761, 797, 802, 970, 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 264, 460
HpyF3I CTNAG 5 cut(s) 120, 294, 517, 890, 933
Hsp92II CATG 6 cut(s) 358, 542, 623, 883, 923, 1034
KspAI GTTAAC 1 cut(s) 790
Kzo9I GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
LmnI GCTCC 4 cut(s) 161, 232, 456, 560
Lsp1109I GCAGC 3 cut(s) 118, 227, 422
LweI GCATC 3 cut(s) 418, 634, 858
MaeI CTAG 3 cut(s) 41, 305, 414
MaeIII GTNAC 2 cut(s) 105, 278
MalI GATC 8 cut(s) 48, 291, 434, 723, 815, 986, 990, 1055
MboI GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
MboII GAAGA 2 cut(s) 504, 731
MflI RGATCY 2 cut(s) 289, 721
MhlI GDGCHC 4 cut(s) 166, 348, 461, 565
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 4 cut(s) 143, 362, 448, 1024
MluNI TGGCCA 1 cut(s) 597
MnlI CCTC 8 cut(s) 206, 264, 303, 378, 526, 583, 741, 942
Mox20I TGGCCA 1 cut(s) 597
MroXI GAANNNNTTC 1 cut(s) 66
MscI TGGCCA 1 cut(s) 597
MseI TTAA 5 cut(s) 170, 494, 789, 861, 999
MslI CAYNNNNRTG 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 597
MspA1I CMGCKG 2 cut(s) 131, 914
MspI CCGG 3 cut(s) 177, 276, 780
MspR9I CCNGG 4 cut(s) 327, 672, 753, 1067
MvaI CCWGG 4 cut(s) 327, 672, 753, 1067
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 3 cut(s) 224, 264, 460
NdeI CATATG 1 cut(s) 313
NdeII GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 6 cut(s) 358, 542, 623, 883, 923, 1034
NmuCI GTSAC 1 cut(s) 105
NruI TCGCGA 1 cut(s) 258
PceI AGGCCT 2 cut(s) 66, 675
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 77, 185
PkrI GCNGC 4 cut(s) 133, 217, 412, 913
Ple19I CGATCG 1 cut(s) 987
Psp124BI GAGCTC 2 cut(s) 166, 461
Psp6I CCWGG 4 cut(s) 325, 670, 751, 1065
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 4 cut(s) 325, 670, 751, 1065
PspPI GGNCC 3 cut(s) 174, 728, 1046
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 2 cut(s) 289, 721
PvuI CGATCG 1 cut(s) 987
RruI TCGCGA 1 cut(s) 258
RsaI GTAC 3 cut(s) 54, 951, 1071
RsaNI GTAC 3 cut(s) 53, 950, 1070
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 2 cut(s) 166, 461
SaqAI TTAA 5 cut(s) 170, 494, 789, 861, 999
SatI GCNGC 4 cut(s) 132, 216, 411, 912
Sau3AI GATC 8 cut(s) 46, 289, 432, 721, 813, 984, 988, 1053
Sau96I GGNCC 3 cut(s) 174, 728, 1046
ScrFI CCNGG 4 cut(s) 327, 672, 753, 1067
SduI GDGCHC 4 cut(s) 166, 348, 461, 565
SfaNI GCATC 3 cut(s) 418, 634, 858
SinI GGWCC 3 cut(s) 174, 728, 1046
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
Sse9I AATT 4 cut(s) 143, 362, 448, 1024
SseBI AGGCCT 2 cut(s) 66, 675
SsiI CCGC 3 cut(s) 129, 454, 912
SspMI CTAG 3 cut(s) 41, 305, 414
SstI GAGCTC 2 cut(s) 166, 461
StuI AGGCCT 2 cut(s) 66, 675
StyD4I CCNGG 4 cut(s) 325, 670, 751, 1065
StyI CCWWGG 1 cut(s) 1049
TaaI ACNGT 2 cut(s) 765, 1012
TaqI TCGA 1 cut(s) 987
TaqII GACCGA 1 cut(s) 17
TasI AATT 4 cut(s) 143, 362, 448, 1024
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 2 cut(s) 77, 185
Tru1I TTAA 5 cut(s) 170, 494, 789, 861, 999
Tru9I TTAA 5 cut(s) 170, 494, 789, 861, 999
TscAI CASTG 4 cut(s) 156, 402, 768, 804
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 3 cut(s) 131, 215, 410
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 2 cut(s) 230, 732
TspRI CASTG 4 cut(s) 156, 402, 768, 804
VpaK11BI GGWCC 3 cut(s) 174, 728, 1046
XagI CCTNNNNNAGG 3 cut(s) 60, 372, 1070
XapI RAATTY 2 cut(s) 362, 448
XcmI CCANNNNNNNNNTGG 2 cut(s) 355, 1047
XmiI GTMKAC 2 cut(s) 633, 747
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 3 cut(s) 41, 305, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.