MD05G1249300.v1.1

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005784
Physical Location & Seq
Reverse (-)
38084737 .. 38095663
10927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1249300.v1.1.491

Sequence Viewer

Length: 7029 bp
ATGAAGGAAGACAGTTCGTCACCTAGTAAAATGATAAACAGAAATTGGGTCTTGAAACGAAAACGTAGAAAGCTTCCTCATGGACCTGATCAGTCTAATGGCAAAGAAGACACTTCAGCGGCCTCAGAATCGCCAGGGAAGACTTCTTCTTCAGCTAAGCGCAAGCTGAAAAATGAATTAATTTCTGAGCGGTTTCAATCCAAGAAGAAAGGAAATGACGGGTATTTCTACGAGTGTGTGGTCTGTGACCTTGGCGGTAACTTGTTGTGTTGTGATAGCTGTCCCCGAACCTATCATCTCCAGTGTCTTAATCCACCTCTTAAGCGCATTCCGAATGGGAAGTGGCAATGTACAACTTGCTGTCAGAAAAGAGATCAGAAAAGTAATCTGCTTGAACCCAGAAACTTTTTGACAGAGACCATCTCAAAACGAGCAAGAACAAAACTTGTCACTACTAAGTCCAAAACTGGAATGAAGTCATCCGACAGGGAGAAAGTATCACAGATTTTTGGAAACTCTATTGTTGCAAAGAAAAGATCTTCAAGCAAAGGAAAAGCTGTTTTAACCCATCGAGTCAAATCCTTAGAGAAGAACTCCCGGATAGATATATGTAGCACCAAGCCGACTCATTCAACAGTTGGTGGTTCTGCAGACGGTATTTCATCATGTGTGAATGTTGATGATGAAAAAAGATCCAGCATTGTCCCAGAAGAGGATTCCACAGACAGAAAGTCGAGCTCTCCTGCTAAAGAAGTTGCATCTCATTCTAAAGTTACATTGTCAGAGACGAATGATGAAGCGCCTGAGGCATCTGCCTCTCCTGATGTGAAGCCGGATCCATCTTGTACTGATGGATCTCCACATAAGACCATTGTTCTTGCAATCAGTGCTACCACCGACAAGGCTAAAAAAAGAAAACACAAAGGAAATAATGATAAGAGTAAAAAGAAGCCCAGGACTGATAAGGGAAAGTCTGTTGGTATTTCCAAACAATCTGGATCTAAAGCAAATACTACAAAACCGAGGATTGATAAAGCACCTCGTAAGCATAAATCTATCAACCATGGGGTTTCTGCTATTTTGTCAAGAGAAGAAATTGGAACCAAGAAGTCAGATGTCCAAAGCAGAGACGAGGAGCTTCCTGAGGGAGCAAAAGACTCATCACATAATGCAGATAAAGCTGGAAGTCATGTAGTTGAAACAACAATTTGTAGAGACAGTTTTACTGCTCAACCTCTGCAGGTTGATCGGGTTTTGGGATGTCGAGTTCAGGGTGATAATGCTCAATCTTCTCGTCAGTTATGTGTGACAGCTGCCCCTGGCCTGTGTCCTGCTGATTTGCAAGTTTCTGAGATTCAAAACAGACTATCAGATGGAAATTCTGCTTTTGATAATGACATGGATGTCGGAGCTGCGGACAGTGTTGTCAATGGTGCTGATGGGGATGAAAGCATGAAGGATGAAAATCTCACTGAGGTTTGCGAAAATGTTGTCGGTGGTGCTGATGGGGATGAAAGCACAAAGGATGATGTTCGAGTGGACAAATTACATGTGTACAGAAGATCTGTGAACAAAGAAGGTAAAAAAGCTAATTCCATGGATTTGTTGAGGACAGGTGCTAAGGATTCGAGTCCTGCTACCATAATTTGTAAAGATCAAGATGAATCTGCTGTAACTGCAGATGATTCAGGAAAAAACCCTGAAAAAACAGCGACTATCAAAGTTAGTTTGAAAAGTCATGATGATGATGAGGTTCCTGAAATTGAAATGCATTTATCTCCTGACACCCAAGATAAACAAGATGTAGATACAGAAACTGGAATCAGCAGCAGCCCTCAAACCAAAATTGAGGAGCCTTCACTAGCTGAGCCTGCAGGTGGTAGTGATGGGATGGTATTGTATGAATTTTTAGTTAAGTGGGTGGGTAGTTCTCATATTCATAATAGTTGGATTTCTGAATCTGAGCTAAAATCCTTGGCCAAGAGAAAATTAGAAAATTACAAGGCTAAGTATGGAACTGCTGTTATAAATATATGTGAGGAACGTTGGAAGCAACCACAGCGGGTGATTGGTCTCCGTGGTGTTGAAAATGGTTTGGGTGAAGCTTTCATAAAGTGGAATGGTCTCCCTTACGATGAATGCACTTGGGAAAGACTGGATGAGCCTGTCATTAAAAATTCCCAGAATCTGATTGATCAGTTTTATCAGTTTGAACGCCAAACACTGGAAAACAATGCTTCTAAGGATGATTCATCAAAGGGGAAGGTTAGTTGTCAGCAAAGTGAAATAGTTACTCTGACAGAGCAGCCTAAGGAACTGAAGGGTATATTGTTTCCTCATCAGCTTGAAGCTCTGAATTGGTTGCGGAAATGCTGGCATAAATCCAAGAATGTAATACTGGCCGATGAGATGGGGCTTGGTAAAACTGTGTCCGCTTGTGCTTTTATTTCATCATTATACGTCGAGTTCAAAGCCACTCTCCCTTGTTTAGTCTTGGTTCCACTTTCCACAATGCGTAATTGGCTTGCTGAGTTTGCATTATGGGCACCCGAATTGAATGTCGTGGAATATCATGGGTGTGCTAAAGCACGAACCATAATACGCCAGCATGAATGGCATGCTAGTGATCCAAATTCATTGAATAAGAAAACATGTGCGTATAAATTTAATGTTCTTTTAACTACATATGAAATGGTTCTTGCTGATTCCTCACATCTCCGCGGGGTTCCTTGGGAAGTTCTCATAGTTGATGAGGGTCATCGTTTGAAAAATTCGGGAAGTAAGCTCTTCAGCTTGCTTAACTCCTTGTCTTTCCAACATCGCGTACTGTTGACTGGTACCCCTCTTCAGAACAACATCGGTGAAATGTATAACTTGCTTAACTTCTTGCAGCCGGCATCATTCCCTTCCCTATCTGCATTTGAGGAAAGGTTTAATGATCTTACAACTGCAGAAAAAGTGGATGAATTGAAAAAACTTGTTGCTCCACATATGCTTCGGAGGCTTAAAAAGGACGCTATGCAAAATATCCCCCCCAAGACCGAACGGATGGTTCCTGTTGAGCTGTCCTCTATCCAAGCTGAATACTACCGCGCAATGCTGACAAAGAATTATCAGATATTGCGGAATATTGGCAAAGGGGTGGCACAGCAGTCAATGCTGAACATAGTAATGCAGTTAAGGAAAGTCTGCAATCATCCGTATCTCATACCAGGCACTGAACCGGACTCTGGGTCTGCTGAGTTCCTTCATGAAATGCGAATTAAAGCTTCAGCCAAGTTGACTCTGTTGCATTCTATGCTTAAGATCTTGCACAAAGAGGGTCACAGAGTCCTCATCTTTTCACAGATGACCAAGCTGCTAGATATCCTTGAGGATTATTTGGCTATAGAATTTGGACCTAAAACATATGAGAGAGTAGACGGCTCTGTTTCAGTGACTGAGCGTCAATCTGCAATTGCGCGTTTTAACCAAGATCAAAGCCGATTTGTCTTTTTGTTATCAACACGCTCTTGTGGCCTTGGTATCAATTTGGCAACAGCAGACACTGTCATTATCTATGATTCTGATTTCAACCCGCACGCTGATATTCAAGCTATGAATCGAGCACATCGAATTGGACAGTCAAAACGACTTTTGGTGTATAGGCTTGTTGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAGAAGAAACTTATGCTTGATCAGCTTTTTGTGAACAAGTCGGGATCCCAAAAAGAAGTGGAGGATATTATAAAATGGGGAACTGAGGAGCTTTTTAATGATTCTCCAATTACTGATGGAAAAGATACCGATGAAAATAACAGTAACAAAGATGAGGCAGTGACAGATGTTGAGCATAAGCATAGGAAGCGGACTGGTGGCTTGGGGGATGTGTATACAGATAAATGTACAGATAGCAGCAACAAGATTGTATGGGATGAAAGTGCAATTTCAAAGTTACTTGACCGTTCAGACCTTCAGTCTGGTTCAAGTGATATTGCTGAAGGAGAAATGGAAAATGATATGCTTGGCTCAGTAAAGGCCATCGAATGGAATGAGGAGCCTGCTGAAGAGCAGGGAGTTGAATCATCTCCAGGTGCAAGTGATGATACTGGGGTACCAAATACTGAAAGGAAAGAGGATAGCATGGTTACGGAAGAAAATGAATGGGACAGACTTTTGCGTCTTAGATGGGAGAAATATCAAAGTGAGGAAGAAGCGGCTCTTGGTCGTGGGAAGCGCCTGCGGAAAGCTGTTTCTTACAGGGAAGCATATGGTGCACACCCAACTGAAACATTAAGTGAGGGTGCTGACGAGGAGCATGAGCCCGAACCAGAGCCAGAGAGGGAGTACACACCTGCTGGACGAGCTTTAAGGGACAAGTTTGCTAAGCTCCGAGCTAGACAAAAAGAGCGGCTTGCTCAGAGGAATGCAGTTGAAGAACCTCAACCTAGTGAAGGACCGCCTGAGTCACTTCCTCAGGGTCCCACCAACACTTCCAAAGATGGCGATCAAGCAACTGAATTAGTTCAGTTTTTCAGAGAGAGACCTTCAGTAATTGACTTGGAGAACGATAAGTTAGATGCTCCCAAGGCCAAGACTGATTCTCCCTCACGATTGGGCCGGCTATTGAAGCATAAAAACAGTCACCTTGGTCTTTCTGTTAATTCTCTTGACTACATGTCACCTGACATGTTCCTTCCCAATCACCAGGTTCTGGGAACAAGCTTACTGCCCTCAAATAATTTATTACCTGTTCTGGGACTCTGTGCTCCCAATGCTAGTCAAATAGTATCATCAAATAAGAAATTCTCAAGGTCAAACGGCAGACAAAAAGGAGGTCGGCCAGAGTTTCCATTTAGTCTGGCTCCTCAGTCTGGGACTTTGAACGAGACAGAAGTAAATGGTGATGACACGAAACTATCAGATGCACCAGCTGAAGTATCACATCTCAAGAATAAGTTGAATAGCATCCCAAATGGTGGTTTCCCATTTAGGCCGTATCCTCCGACTTTTCAAGGGAATAATCATGATCGTCCAGAAAGTTCCAGTGCTTCCTTTTCTGATTTCCAAGAAAAGATGGCATTGCCTAACTTACCATTCGATGAGAAATTGCTACCAAGATTCCCACTTGGAGCAAAGAGCATGCCGAGTCCACATCTGGACTTCTTATCTAATCTATCATTAGGTAGCAGACTTGAATCTGCTGGTGGATCCTTGCAAGAACTTCCAACAATGTCATTGTTTCCAAATTTACAGTTGCCACCAGATGCACCCAGATATAATCAGCAAGACAGAGATGTGCCTCCCTCATTTGGTTTGGGGCACATGCCAACTAATTTTCCGTCTTTACCTGATAACCACCGGAAGGTGCTCGAAAACATAATGATGAGGACTGGTCCTGGATCAAGCAACTTATTTAAAAATAAATTTAAAGCAGATATCTGGACGGAAGATGAACTTGATTATCTATGGATTGGTGTTCGTAGGCATGGAAGGGGCAATTGGGATGCAATGCTGAGAGACCCAAGGTTGAAGTTCTCAACGTTTAAAACTTCGGAAGATTTGTCAGCTAGGTGGGAGGAGGAACAACTCAAGATTTTAGACGGGTCAGCTTTTGCAGTGTCTAAGTCAACCAAAAAGGCTGCTAAATCCTTACAGTTTCCAAGCATATCTGATGGAATGATGGCACGGGCACTGCATAGTAGTAGACTTGTTACACCACCAAAGTTTCAATCCCATTTGACAGATATAAAGCTGGGTTTTACTGATCTTTCAACTGGCTTTCCACATTTTGAATCATCAGATAGACTCGGTTTGCAAAATGAGCAATATCCCCCTATTCCATCTTGGTTTCATGATAAATTCAGGACTAATTTTTCCGGAGATTCTGCTGCTGTAGCTTCTGACAGAGCAGGGACATCTTCCAGTGTTCCTGCTGAGCAGCCATTTGTTGTCACTTCTTTTGGTACCAGTTGCTTGGGTTCGTTAGGAGGCTTGAATTCCTCAAGCAGCTATGATGTGCAGAAGAATGAAGATGAACAGGGTGCCCCTAGGTACGGGAAGTTGCCTAGTCTGCTTGATAGATCACTGAATGCTGTACGTGATATGAATAATAATTTGGCAAGAGGTGAACCCTCCAGTTCAGGATTGCTGCCTAATCTCAAGAGAGGAATCTTAAAGGGAGATGATGTTGCTGGAAGTAGTTCTTCGAAGAACACGCTACCCCATTGGCTACGGGAAGCTGTAAGTGCTCCTGCTAAACCCCCAGTACCTGATCTGCCACCCACAGTGATGGCAATAGCTCAATCCGTCCGGTTGTTATATGGGGAGGATAAGCGAACGATTCCTCCATTTGTGATTCCCGGCCCACCTCCTTCGCTACCAAAGGATCCAAGACGGAGTCTAAAGAAGAAAAGAAAGCTTAAATCACGTCTATTCAGGCGGGTTCAACTAGACGCTGGAAGCAGCCAGGACTTTCAGAGCAGGCACTTTGGTGGCAATGCTTCAAGCTCCATTCCTATGGCCCCATCATTTCCATTTCTTTCACAAGCAATGGCTGCAACTTCAGGCTTTTCACGGATTGAATCCGACCTCGGTGAGCCTCTGAGTTTAAATGTGGTAAACCCATCATCTGCAGCGCCACATCTGAATCAGCACAAGAAAACAACCATGGGATTGTCGCCTTCCCCTGAAGTGCTTCAATTGGTAGCATCCTGTGTTGCTCCAGGTTCACACCTGTCAGCAGCTTCCGGCATGGCAAGCTCAAGCGTCCGTGACACAAAACCCTCATTGCCGAACTCTGTTGACCAAGTAGAACTCTTGGACTCTCAAACTGCGACTGCAGTGGTAAGAACGGAGGCCAAGCAGGGCTCGCCTGTTAGAACATGTGGTACTCAAAGTGCGGATTCTAGCAAGACAGAGTCAGATCCCTCTAGGACAGAACGACCCGATGTAGAGGAAATTTCTTCTGAGGGCACCGTCTCGGATCATCCCATGAGTGATCGGGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

2343

Amino Acids

258.64

Weight (kDa)

7.11

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 79 - 120 7.4e-09 PHD-finger
Chromo PF00385 685 - 736 8.9e-09 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 777 - 948 2.1e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 780 - 1069 2.4e-79 SNF2-related domain
HDA2-3 PF11496 1017 - 1234 7.7e-13 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 1093 - 1207 1.6e-23 Helicase conserved C-terminal domain
DUF1087 PF06465 1383 - 1427 2.9e-06 CHD subfamily II, DUF1087
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2027, 3764
AarI CACCTGC 2 cut(s) 1865, 4378
AasI GACNNNNNNGTC 3 cut(s) 1403, 1424, 4194
Acc36I ACCTGC 3 cut(s) 1231, 1865, 4378
Acc65I GGTACC 3 cut(s) 2837, 4129, 5981
AccB1I GGYRCC 6 cut(s) 2545, 2837, 4129, 5981, 6059, 6989
AccB7I CCANNNNNTGG 4 cut(s) 2224, 4062, 4729, 4994
AccBSI CCGCTC 2 cut(s) 190, 4426
AccI GTMKAC 3 cut(s) 3420, 3908, 5722
AccII CGCG 4 cut(s) 2721, 2823, 3093, 3463
AccIII TCCGGA 1 cut(s) 5894
AclI AACGTT 2 cut(s) 2044, 5558
AcoI YGGCCR 3 cut(s) 1977, 2400, 4856
AflII CTTAAG 2 cut(s) 320, 3302
AflIII ACRYGT 5 cut(s) 1549, 2651, 4692, 4704, 6899
AhdI GACNNNNNGTC 6 cut(s) 16, 730, 3232, 3444, 3991, 4693
AjiI CACGTC 1 cut(s) 6446
AjnI CCWGG 9 cut(s) 133, 953, 1318, 3211, 4105, 4722, 5413, 6483, 6739
AjuI GAANNNNNNNTTGG 6 cut(s) 4221, 4253, 5500, 5532, 6116, 6148
AleI CACNNNNGTG 1 cut(s) 6507
AloI GAACNNNNNNTCC 4 cut(s) 1251, 1283, 6346, 6378
Alw21I GWGCWC 6 cut(s) 740, 3610, 4294, 4786, 5388, 6268
Alw44I GTGCAC 1 cut(s) 4290
AlwNI CAGNNNCTG 7 cut(s) 1817, 2188, 3218, 3440, 3548, 4729, 6287
Aor13HI TCCGGA 1 cut(s) 5894
ApaLI GTGCAC 1 cut(s) 4290
ArsI GACNNNNNNTTYG 6 cut(s) 1476, 1508, 2544, 2576, 4837, 4869
AseI ATTAAT 1 cut(s) 179
Asp700I GAANNNNTTC 4 cut(s) 2694, 4539, 6217, 6711
Asp718I GGTACC 3 cut(s) 2837, 4129, 5981
AspA2I CCTAGG 1 cut(s) 6065
AspLEI GCGC 8 cut(s) 162, 327, 802, 3095, 3463, 3678, 4254, 6655
AspS9I GGNCC 8 cut(s) 83, 3398, 4472, 4496, 4633, 5411, 6380, 6538
AsuC2I CCSGG 2 cut(s) 598, 6378
AsuII TTCGAA 1 cut(s) 6224
AvaII GGWCC 5 cut(s) 83, 3398, 4472, 4496, 5411
AvrII CCTAGG 1 cut(s) 6065
AxyI CCTNAGG 4 cut(s) 804, 1143, 2310, 4491
BaeGI GKGCMC 6 cut(s) 2548, 4294, 5340, 5710, 6064, 6992
BaeI ACNNNNGTAYC 4 cut(s) 4113, 4113, 4146, 4146
BalI TGGCCA 1 cut(s) 1979
BamHI GGATCC 4 cut(s) 835, 3737, 5225, 6403
BanI GGYRCC 6 cut(s) 2545, 2837, 4129, 5981, 6059, 6989
BanII GRGCYC 3 cut(s) 740, 4341, 6887
BbsI GAAGAC 3 cut(s) 15, 114, 146
Bbv12I GWGCWC 6 cut(s) 740, 3610, 4294, 4786, 5388, 6268
BceAI ACGGC 3 cut(s) 3439, 4852, 4996
BcgI CGANNNNNNTGC 2 cut(s) 1229, 1263
BciT130I CCWGG 9 cut(s) 135, 955, 1320, 3213, 4107, 4724, 5415, 6485, 6741
BciVI GTATCC 1 cut(s) 5025
BclI TGATCA 3 cut(s) 88, 2194, 3712
BcnI CCSGG 2 cut(s) 598, 6378
BfmI CTRYAG 9 cut(s) 648, 1238, 1677, 1872, 2949, 3387, 5910, 6648, 6855
BfoI RGCGCY 3 cut(s) 803, 4255, 6656
BfrI CTTAAG 2 cut(s) 320, 3302
BfuAI ACCTGC 3 cut(s) 1231, 1865, 4378
BfuI GTATCC 1 cut(s) 5025
BglII AGATCT 3 cut(s) 536, 1562, 3306
BlnI CCTAGG 1 cut(s) 6065
BlpI GCTNAGC 4 cut(s) 156, 1866, 4401, 5952
Bme18I GGWCC 5 cut(s) 83, 3398, 4472, 4496, 5411
BmeRI GACNNNNNGTC 6 cut(s) 16, 730, 3232, 3444, 3991, 4693
BmgBI CACGTC 1 cut(s) 6446
BmgT120I GGNCC 8 cut(s) 83, 3398, 4472, 4496, 4633, 5411, 6380, 6538
BmrI ACTGGG 2 cut(s) 4134, 6275
BmuI ACTGGG 2 cut(s) 4134, 6275
BpiI GAAGAC 3 cut(s) 15, 114, 146
BplI GAGNNNNNCTC 6 cut(s) 407, 439, 578, 610, 3053, 3085
BpmI CTGGAG 4 cut(s) 284, 4089, 6136, 6723
Bpu10I CCTNAGC 1 cut(s) 1620
Bpu1102I GCTNAGC 4 cut(s) 156, 1866, 4401, 5952
Bpu14I TTCGAA 1 cut(s) 6224
BpuEI CTTGAG 7 cut(s) 3392, 4810, 4949, 5591, 6004, 6161, 6763
BpuMI CCSGG 2 cut(s) 598, 6378
BsaAI YACGTR 1 cut(s) 6116
BsaBI GATNNNNATC 2 cut(s) 1464, 4521
BsaI GGTCTC 5 cut(s) 410, 2078, 2129, 4552, 5529
BsaWI WCCGGW 4 cut(s) 3223, 5376, 5894, 6327
BsaXI ACNNNNNCTCC 6 cut(s) 4603, 4633, 6189, 6219, 6346, 6376
Bse118I RCCGGY 2 cut(s) 2893, 4635
Bse21I CCTNAGG 4 cut(s) 804, 1143, 2310, 4491
Bse3DI GCAATG 7 cut(s) 353, 3102, 5096, 5532, 6520, 6573, 6803
Bse8I GATNNNNATC 2 cut(s) 1464, 4521
BseAI TCCGGA 1 cut(s) 5894
BseBI CCWGG 9 cut(s) 135, 955, 1320, 3213, 4107, 4724, 5415, 6485, 6741
BseJI GATNNNNATC 2 cut(s) 1464, 4521
BseMI GCAATG 7 cut(s) 353, 3102, 5096, 5532, 6520, 6573, 6803
BseRI GAGGAG 7 cut(s) 1148, 1865, 3794, 4085, 4343, 4872, 5609
BseSI GKGCMC 6 cut(s) 2548, 4294, 5340, 5710, 6064, 6992
BseYI CCCAGC 1 cut(s) 5770
BsgI GTGCAG 1 cut(s) 6056
Bsh1236I CGCG 4 cut(s) 2721, 2823, 3093, 3463
BshNI GGYRCC 6 cut(s) 2545, 2837, 4129, 5981, 6059, 6989
BsiHKAI GWGCWC 6 cut(s) 740, 3610, 4294, 4786, 5388, 6268
BslFI GGGAC 8 cut(s) 267, 689, 4196, 4403, 4482, 4788, 4906, 5944
BsmBI CGTCTC 3 cut(s) 779, 1122, 7000
BsmFI GGGAC 8 cut(s) 267, 689, 4196, 4403, 4482, 4788, 4906, 5944
BsmI GAATGC 5 cut(s) 327, 2144, 3292, 4447, 6112
Bso31I GGTCTC 5 cut(s) 410, 2078, 2129, 4552, 5529
Bsp119I TTCGAA 1 cut(s) 6224
Bsp13I TCCGGA 1 cut(s) 5894
Bsp1407I TGTACA 3 cut(s) 350, 1554, 3920
Bsp1720I GCTNAGC 4 cut(s) 156, 1866, 4401, 5952
Bsp19I CCATGG 3 cut(s) 1063, 1596, 6684
BspEI TCCGGA 1 cut(s) 5894
BspFNI CGCG 4 cut(s) 2721, 2823, 3093, 3463
BspHI TCATGA 4 cut(s) 1738, 3250, 5041, 5869
BspMAI CTGCAG 7 cut(s) 652, 1242, 1681, 1876, 2953, 6652, 6859
BspMI ACCTGC 3 cut(s) 1231, 1865, 4378
BspQI GCTCTTC 3 cut(s) 2792, 3666, 4077
BspT104I TTCGAA 1 cut(s) 6224
BspT107I GGYRCC 6 cut(s) 2545, 2837, 4129, 5981, 6059, 6989
BspTI CTTAAG 2 cut(s) 320, 3302
BspTNI GGTCTC 5 cut(s) 410, 2078, 2129, 4552, 5529
BsrBI CCGCTC 2 cut(s) 190, 4426
BsrDI GCAATG 7 cut(s) 353, 3102, 5096, 5532, 6520, 6573, 6803
BsrFI RCCGGY 2 cut(s) 2893, 4635
BsrGI TGTACA 3 cut(s) 350, 1554, 3920
BssAI RCCGGY 2 cut(s) 2893, 4635
BssNAI GTATAC 1 cut(s) 3909
Bst1107I GTATAC 1 cut(s) 3909
Bst2UI CCWGG 9 cut(s) 135, 955, 1320, 3213, 4107, 4724, 5415, 6485, 6741
Bst6I CTCTTC 5 cut(s) 705, 2792, 2850, 3666, 4077
BstAFI CTTAAG 2 cut(s) 320, 3302
BstAPI GCANNNNNTGC 5 cut(s) 887, 3157, 3298, 4289, 6572
BstAUI TGTACA 3 cut(s) 350, 1554, 3920
BstBAI YACGTR 1 cut(s) 6116
BstBI TTCGAA 1 cut(s) 6224
BstDSI CCRYGG 5 cut(s) 1063, 1596, 2077, 2719, 6684
BstENI CCTNNNNNAGG 1 cut(s) 4467
BstFNI CGCG 4 cut(s) 2721, 2823, 3093, 3463
BstH2I RGCGCY 3 cut(s) 803, 4255, 6656
BstHHI GCGC 8 cut(s) 162, 327, 802, 3095, 3463, 3678, 4254, 6655
BstNI CCWGG 9 cut(s) 135, 955, 1320, 3213, 4107, 4724, 5415, 6485, 6741
BstNSI RCATGY 8 cut(s) 1553, 2621, 2655, 4696, 4708, 5161, 5344, 6903
BstSFI CTRYAG 9 cut(s) 648, 1238, 1677, 1872, 2949, 3387, 5910, 6648, 6855
BstSLI GKGCMC 6 cut(s) 2548, 4294, 5340, 5710, 6064, 6992
BstUI CGCG 4 cut(s) 2721, 2823, 3093, 3463
BstV2I GAAGAC 3 cut(s) 15, 114, 146
BstXI CCANNNNNNTGG 3 cut(s) 5992, 6307, 6535
BstZ17I GTATAC 1 cut(s) 3909
Bsu36I CCTNAGG 4 cut(s) 804, 1143, 2310, 4491
BsuI GTATCC 1 cut(s) 5025
BtgI CCRYGG 5 cut(s) 1063, 1596, 2077, 2719, 6684
BtgZI GCGATG 1 cut(s) 2804
BtrI CACGTC 1 cut(s) 6446
BtsI GCAGTG 4 cut(s) 3858, 5640, 5708, 6864
BveI ACCTGC 3 cut(s) 1231, 1865, 4378
CaiI CAGNNNCTG 7 cut(s) 1817, 2188, 3218, 3440, 3548, 4729, 6287
CciI TCATGA 4 cut(s) 1738, 3250, 5041, 5869
CfoI GCGC 8 cut(s) 162, 327, 802, 3095, 3463, 3678, 4254, 6655
Cfr10I RCCGGY 2 cut(s) 2893, 4635
Cfr13I GGNCC 8 cut(s) 83, 3398, 4472, 4496, 4633, 5411, 6380, 6538
Cfr42I CCGCGG 1 cut(s) 2722
CseI GACGC 5 cut(s) 3023, 3434, 4184, 6479, 6772
CsiI ACCWGGT 1 cut(s) 4722
DraI TTTAAA 4 cut(s) 5434, 5446, 5563, 6627
DrdI GACNNNNNNGTC 3 cut(s) 1403, 1424, 4194
DriI GACNNNNNGTC 6 cut(s) 16, 730, 3232, 3444, 3991, 4693
DseDI GACNNNNNNGTC 3 cut(s) 1403, 1424, 4194
EaeI YGGCCR 3 cut(s) 1977, 2400, 4856
Eam1104I CTCTTC 5 cut(s) 705, 2792, 2850, 3666, 4077
Eam1105I GACNNNNNGTC 6 cut(s) 16, 730, 3232, 3444, 3991, 4693
EarI CTCTTC 5 cut(s) 705, 2792, 2850, 3666, 4077
Ecl136II GAGCTC 1 cut(s) 738
Eco24I GRGCYC 3 cut(s) 740, 4341, 6887
Eco31I GGTCTC 5 cut(s) 410, 2078, 2129, 4552, 5529
Eco32I GATATC 2 cut(s) 3367, 5455
Eco47I GGWCC 5 cut(s) 83, 3398, 4472, 4496, 5411
Eco53kI GAGCTC 1 cut(s) 738
Eco81I CCTNAGG 4 cut(s) 804, 1143, 2310, 4491
EcoICRI GAGCTC 1 cut(s) 738
EcoNI CCTNNNNNAGG 1 cut(s) 4467
EcoO109I RGGNCCY 1 cut(s) 4496
EcoRI GAATTC 1 cut(s) 6013
EcoRII CCWGG 9 cut(s) 133, 953, 1318, 3211, 4105, 4722, 5413, 6483, 6739
EcoRV GATATC 2 cut(s) 3367, 5455
EcoT22I ATGCAT 1 cut(s) 1773
EcoT38I GRGCYC 3 cut(s) 740, 4341, 6887
Esp3I CGTCTC 3 cut(s) 779, 1122, 7000
FalI AAGNNNNNCTT 8 cut(s) 4221, 4253, 4413, 4445, 5457, 5489, 6205, 6237
FaqI GGGAC 8 cut(s) 267, 689, 4196, 4403, 4482, 4788, 4906, 5944
FauI CCCGC 4 cut(s) 2055, 2714, 3585, 6450
FauNDI CATATG 4 cut(s) 2686, 2991, 3409, 4285
FbaI TGATCA 3 cut(s) 88, 2194, 3712
FblI GTMKAC 3 cut(s) 3420, 3908, 5722
FriOI GRGCYC 3 cut(s) 740, 4341, 6887
GlaI GCGC 8 cut(s) 161, 326, 801, 3094, 3462, 3677, 4253, 6654
GsaI CCCAGC 1 cut(s) 5774
GsuI CTGGAG 4 cut(s) 284, 4089, 6136, 6723
HaeII RGCGCY 3 cut(s) 803, 4255, 6656
HgaI GACGC 5 cut(s) 3023, 3434, 4184, 6479, 6772
HhaI GCGC 8 cut(s) 162, 327, 802, 3095, 3463, 3678, 4254, 6655
Hin6I GCGC 8 cut(s) 160, 325, 800, 3093, 3461, 3676, 4252, 6653
HinP1I GCGC 8 cut(s) 160, 325, 800, 3093, 3461, 3676, 4252, 6653
HincII GTYRAC 4 cut(s) 2832, 3282, 5646, 6820
HindII GTYRAC 4 cut(s) 2832, 3282, 5646, 6820
HindIII AAGCTT 5 cut(s) 71, 2103, 3267, 4738, 6434
Hpy99I CGWCG 1 cut(s) 2465
HpyCH4IV ACGT 6 cut(s) 64, 2044, 2460, 5558, 6115, 6445
HpySE526I ACGT 6 cut(s) 64, 2044, 2460, 5558, 6115, 6445
HspAI GCGC 8 cut(s) 160, 325, 800, 3093, 3461, 3676, 4252, 6653
KflI GGGWCCC 1 cut(s) 4496
Kpn2I TCCGGA 1 cut(s) 5894
KpnI GGTACC 3 cut(s) 2841, 4133, 5985
KroI GCCGGC 2 cut(s) 2893, 4635
KroNI GCCGGC 2 cut(s) 2895, 4637
Ksp22I TGATCA 3 cut(s) 88, 2194, 3712
KspI CCGCGG 1 cut(s) 2722
LguI GCTCTTC 3 cut(s) 2792, 3666, 4077
MabI ACCWGGT 1 cut(s) 4722
MaeII ACGT 6 cut(s) 64, 2044, 2460, 5558, 6115, 6445
MbiI CCGCTC 2 cut(s) 190, 4426
MfeI CAATTG 3 cut(s) 3456, 5515, 6716
MlsI TGGCCA 1 cut(s) 1979
MluNI TGGCCA 1 cut(s) 1979
MmeI TCCRAC 8 cut(s) 507, 1387, 1928, 2027, 2839, 5045, 5267, 6627
Mox20I TGGCCA 1 cut(s) 1979
Mph1103I ATGCAT 1 cut(s) 1773
MroI TCCGGA 1 cut(s) 5894
MroNI GCCGGC 2 cut(s) 2893, 4635
MroXI GAANNNNTTC 4 cut(s) 2694, 4539, 6217, 6711
MscI TGGCCA 1 cut(s) 1979
MslI CAYNNNNRTG 8 cut(s) 1743, 2577, 2622, 3170, 5399, 6305, 6507, 6533
Msp20I TGGCCA 1 cut(s) 1979
MspA1I CMGCKG 5 cut(s) 119, 1313, 2062, 2721, 4949
MspCI CTTAAG 2 cut(s) 320, 3302
MunI CAATTG 3 cut(s) 3456, 5515, 6716
Mva1269I GAATGC 5 cut(s) 327, 2144, 3292, 4447, 6112
MvaI CCWGG 9 cut(s) 135, 955, 1320, 3213, 4107, 4724, 5415, 6485, 6741
MvnI CGCG 4 cut(s) 2721, 2823, 3093, 3463
NaeI GCCGGC 2 cut(s) 2895, 4637
NciI CCSGG 2 cut(s) 598, 6378
NcoI CCATGG 3 cut(s) 1063, 1596, 6684
NdeI CATATG 4 cut(s) 2686, 2991, 3409, 4285
NgoMIV GCCGGC 2 cut(s) 2893, 4635
NmeAIII GCCGAG 1 cut(s) 5187
NsiI ATGCAT 1 cut(s) 1773
NspI RCATGY 8 cut(s) 1553, 2621, 2655, 4696, 4708, 5161, 5344, 6903
NspV TTCGAA 1 cut(s) 6224
OliI CACNNNNGTG 1 cut(s) 6507
PaeI GCATGC 2 cut(s) 2621, 5161
PagI TCATGA 4 cut(s) 1738, 3250, 5041, 5869
PaqCI CACCTGC 2 cut(s) 1865, 4378
PciI ACATGT 5 cut(s) 1549, 2651, 4692, 4704, 6899
PciSI GCTCTTC 3 cut(s) 2792, 3666, 4077
PcsI WCGNNNNNNNCGW 1 cut(s) 3610
PctI GAATGC 5 cut(s) 327, 2144, 3292, 4447, 6112
PdiI GCCGGC 2 cut(s) 2895, 4637
PdmI GAANNNNTTC 4 cut(s) 2694, 4539, 6217, 6711
PflFI GACNNNGTC 3 cut(s) 3548, 6414, 6934
PflMI CCANNNNNTGG 4 cut(s) 2224, 4062, 4729, 4994
PfoI TCCNGGA 2 cut(s) 596, 5413
Ppu21I YACGTR 1 cut(s) 6116
PpuMI RGGWCCY 1 cut(s) 4496
PscI ACATGT 5 cut(s) 1549, 2651, 4692, 4704, 6899
PshBI ATTAAT 1 cut(s) 179
PsiI TTATAA 2 cut(s) 2027, 3764
Psp124BI GAGCTC 1 cut(s) 740
Psp1406I AACGTT 2 cut(s) 2044, 5558
Psp5II RGGWCCY 1 cut(s) 4496
Psp6I CCWGG 9 cut(s) 133, 953, 1318, 3211, 4105, 4722, 5413, 6483, 6739
PspFI CCCAGC 1 cut(s) 5770
PspGI CCWGG 9 cut(s) 133, 953, 1318, 3211, 4105, 4722, 5413, 6483, 6739
PspPI GGNCC 8 cut(s) 83, 3398, 4472, 4496, 4633, 5411, 6380, 6538
PspPPI RGGWCCY 1 cut(s) 4496
PsrI GAACNNNNNNTAC 2 cut(s) 6889, 6921
PstI CTGCAG 7 cut(s) 652, 1242, 1681, 1876, 2953, 6652, 6859
PstNI CAGNNNCTG 7 cut(s) 1817, 2188, 3218, 3440, 3548, 4729, 6287
PsyI GACNNNGTC 3 cut(s) 3548, 6414, 6934
PvuII CAGCTG 2 cut(s) 1313, 4949
RseI CAYNNNNRTG 8 cut(s) 1743, 2577, 2622, 3170, 5399, 6305, 6507, 6533
SacI GAGCTC 1 cut(s) 740
SacII CCGCGG 1 cut(s) 2722
SapI GCTCTTC 3 cut(s) 2792, 3666, 4077
Sau96I GGNCC 8 cut(s) 83, 3398, 4472, 4496, 4633, 5411, 6380, 6538
SbfI CCTGCAGG 1 cut(s) 1876
SdaI CCTGCAGG 1 cut(s) 1876
SexAI ACCWGGT 1 cut(s) 4722
SfcI CTRYAG 9 cut(s) 648, 1238, 1677, 1872, 2949, 3387, 5910, 6648, 6855
Sfr303I CCGCGG 1 cut(s) 2722
SfuI TTCGAA 1 cut(s) 6224
SgrBI CCGCGG 1 cut(s) 2722
SinI GGWCC 5 cut(s) 83, 3398, 4472, 4496, 5411
SmiMI CAYNNNNRTG 8 cut(s) 1743, 2577, 2622, 3170, 5399, 6305, 6507, 6533
SmlI CTYRAG 9 cut(s) 320, 3302, 3371, 4825, 4964, 5606, 6019, 6176, 6778
SmoI CTYRAG 9 cut(s) 320, 3302, 3371, 4825, 4964, 5606, 6019, 6176, 6778
SphI GCATGC 2 cut(s) 2621, 5161
Sse8387I CCTGCAGG 1 cut(s) 1876
SspI AATATT 1 cut(s) 3130
SstI GAGCTC 1 cut(s) 740
TaiI ACGT 6 cut(s) 67, 2047, 2463, 5561, 6118, 6448
TaqII GACCGA 1 cut(s) 3056
TatI WGTACW 5 cut(s) 350, 845, 1554, 3920, 4362
TauI GCSGC 3 cut(s) 122, 4235, 4429
Tth111I GACNNNGTC 3 cut(s) 3548, 6414, 6934
Van91I CCANNNNNTGG 4 cut(s) 2224, 4062, 4729, 4994
Vha464I CTTAAG 2 cut(s) 320, 3302
VneI GTGCAC 1 cut(s) 4290
VpaK11BI GGWCC 5 cut(s) 83, 3398, 4472, 4496, 5411
VspI ATTAAT 1 cut(s) 179
XagI CCTNNNNNAGG 1 cut(s) 4467
XceI RCATGY 8 cut(s) 1553, 2621, 2655, 4696, 4708, 5161, 5344, 6903
XmaJI CCTAGG 1 cut(s) 6065
XmiI GTMKAC 3 cut(s) 3420, 3908, 5722
XmnI GAANNNNTTC 4 cut(s) 2694, 4539, 6217, 6711
Zsp2I ATGCAT 1 cut(s) 1773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.