MD10G1229800.v1.1

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005784
Physical Location & Seq
Reverse (-)
32663756 .. 32675129
11374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1229800.v1.1.491

Sequence Viewer

Length: 7041 bp
ATGATAAACAGGAATTGGGTCTTGAAACGAAAACGTAGAAAGCTTCCTCATGGACCTGATCAATCTAATGGCAAAGAAGACGCTTCAGTGGCCTCAGAATCTCCAGGGAAGACCTCTTCATCTAAGCGCAAGCTGAAAAATGAATTAATTTCTGAGCGGTTTCAATCAAAGAAGAAGGGAAATGACGGGTATTTCTACGAGTGCGTGGTCTGTGACCTTGGCGGTAACTTGTTATGTTGTGATAGCTGTCCCCGAACCTATCATCTCCAGTGTCTTAATCCACCTCTTAAGCGCATTCCCAATGGGAAGTGGCAATGTCCAACTTGCTGTCAGAAAAGTGATCAGAAAAGTAATCTGCTTGAACCCAGAAACTTTTTGACGGATACCATTTCAAAACGAGCAAGAACAAAACTTGTCACTACGAAGTCCAAAACTGGAATGAAGTCATCTGACAGGGAGAAAGTATCACATAACTTTGGAAACTCCATTGTTGCAAAGAAAAGATCCTCAAGCAAAGGAAAAGCTGTTTTAACCCATGGAGTCAAATCCTTAGAGAAGAACTCCCAGATAGATATATGTAGCACCAAGCCGACTCATTCAACAGTTGGTGGTTCTGCAGACGGTATTTCATCTTGTGTGAATGTTGATGATGAAAAGAGATCCAGCATTGTCCCAGAAGAGAATTCCACAGACAGAAAGTCGAGCTCTCCTGCTAAAGAAGTTTCATCTCATTCTAAAGTTATAGCATCAGAGACGAAGGAAGAAGCTCCTGAGTTATCTGCCTCTCCTGATGTGAAGCCTGATATGTCTTGTACTGATGGATCTCCACGCAAGACCATTGTTCTTGCGATCAGTGCTACAACTGACAAGGCTAAAAAGAGAAAACACAAAGGAAATAATGATAAGAGTAAAAAGAAGCGCAGGAACGATAAGGGGAAGTCTGTTAGTATTTCTGAACAATCTGGATCTAAGGCAAATACTGCAAAACTGAGGATTGGTAAAGCACCTCGTAAGCATAAATCTATTAACCATGGGGTTTCTGCATCTTTGCCAAGAGAAGAAATTGGAACCAAGAAGTCAGATATTCAAAGCAAAGACAAGGAGCTTCCTGAGGGAGCAAACGACTCATCACATAATGCAGATAAAGCTGGAAGTCATGCAGTTGAAACACCAATTTGTAGAGATAGTTTTACTGCTCAACCTCTGCAGGTTGATAGGGTTTTGGGATGTCGAGTTCAGGGTGACAATGCTGAATCTTCTCGTCATATACGTATGACAGCTGCTTCTGACCTGTGTCCTGATGATTTGCAAGTTTCTGAGACTCAAAACAGACTCGCAGATGGAAATTCTGCTTGTGATAACGACATGGATGTCGGAGCTGCTGACAAAATTGTCAATGGTGGTGATGGGGATGAAAATCTTATCGAGGGTTGTGAAAATGTTGTCAGTGGTGCTGATAGGGATGAAAGCATGAAGGATGATAATCTTGTGGAGGGTTGCGAAAATGTTGTCAATGGTGTTGATGGGGATGAAAGCACAAAGGATGATGTTAGAGTGGACAAAATACATGTGTACAGAAGATCTGTGAACAAAGAAGGTAAAAAAGCTAATTCCATGGAAGTGTTGAGGATGGGTGCTAAGGATTTGAGTCCTGCTAACAGAATTTGTAGAGATCAAGAGGAATCTGCTGTAGCTGCAGATGATTCAGTAAAACCCCCCAAAAAAATTGTGACTGCGGAGAATACCGAAGTTAGTCTGAAAAGTCTTGATAATGATGAGGTTCGTGAAATTGAAATGCATGTATCCCTTGACACCGAAGACAAAAAAGATGCAGATACAGAAACTGGACTCAGTAGCAGCCCTCAAACCAAAATTCAGGGGCCCTCACTGGCTGATCTTGCAGGTGGAAGTGATGGGACTGTATCGTATGAATTTTTAGTTAAGTGGGTGGGTAAGTCTCATATTCATAATAGTTGGATCTCTGAATCTGAGCTAAAAGTCTTGGCCAAGAGAAAATTAGAAAATTACAAGGCTAAGTATGGAACTGCTGTTATAAATATCTGTGAGGAACGGTGGAAGCAACCACAGCGGGTGATTGGTCTACGCGGTGTTGAAAATGGTTCAGGTGAAGCTCTGATAAAGTGGACTGGTCTCCCATACGTTGAATGCACTTGGGAAAGATTGGACAAGCCTGTCATTAAAAATTCTCAGAATCTGATCGATCAATTTAATCAGTTTGAGCGCCAAACACTGGAAAACAATGCTTCTAAGGATGATTCATCAAAGGGGAAGGTTAGTTGTCAGCAAAGTGAAATAGTTACTCTGACAGAGCAGCCTAAGGAACTGAAGGGTATATTGTTTCCTCATCAGCTTGAAGCACTTAATTGGTTGCGTAAATGCTGGCATAAATCCAAGAATGTAATACTAGCGGATGAGATGGGGCTTGGTAAAACTGTGTCAGCTTGTGCTTTTATTTCATCGTTATACGTTGAGTTCAAAGCCACTCTGCCTTGTTTAGTCTTGGTTCCACTTTCCACAATGCCTAATTGGCTTGCTGAGTTTTCATTATGGGCTCCTGAATTGAATGTTGTGGAATATCATGGGTGTGCTAAAGCACGGACCATAATTCGCCAGCAAGAATGGCATGCTAGTGATCCAAATACGTTGAATAAGAAAACATCTGCGTATAAATTTAATGTTCTTTTAACTACGTATGAAATGGTTCTTGCTGATTCCTCACATCTCCGCGGGGTTCCTTGGGAAGTTCTCATAGTTGATGAAGGTCATCGTTTGAAAAATTCAGGAAGTAAGCTTTTCAGCTTGCTCAACTCCTTGTCTTTCCAGCATCGCGTACTGTTGACTGGTACCCCTCTTCAGAACAACATTGGTGAAATGTATAACTTGCTTAACTTCTTGCAGCCGGCATCATTCCCTTCCCTTTCTGCATTTGAGGAAAGGTTTAATGATCTTACAACTGCAGAAAAGGTGGATGAATTGAAAAAACTTGTTGCTCCACATATGCTTCGGAGGCTTAAAAAGGATGCTATGCAAAATATCCCCCCCAAGACTGAACGGATGGTTCCTGTTGAGCTGTCCTCTATCCAAGCTGAATACTACCGTGCAATGCTGACAAAGAATTATCAGATATTGCGGAATATTGGAAAAGGGGTGGCACAACAATCCATGCTAAACATCGTAATGCAGTTAAGGAAAGTCTGCAATCATCCGTATCTTATACCTGGCACTGAGCCTGACTCTGGGTCTGCAGAGTTCCTTCATGAAATGCGGATAAAGGCTTCGGCCAAGTTGACTCTGTTGCATTCTATGCTTAAGATTTTGCACAAAGAGGGTCACAGAGTCCTCATCTTTTCACAGATGACCAAGCTGCTAGATATCCTCGAGGATTATTTGGCCATAGAATTTGGACCTAAAACATATGAGAGAGTAGACGGCTCTGTTTCGGTGACTGATCGTCAATCTGCAATTGCACGTTTTAACCAAGATCAAAGTCGATTTGTCTTTTTGTTATCAACACGCTCTTGCGGCCTTGGTATCAATTTGGCAACAGCTGACACTGTCATTATCTATGATTCTGATTTCAACCCGCATGCTGATATTCAAGCTATGAACCGAGCACATCGAATTGGACAGTCAAAACGACTTCTGGTATATCGGCTTGTAGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAAAAGAAACTTATGCTTGATCAGCTTTTTGTGAATAAGTCGGGATCCCAAAAAGAAGTGGAGGATATTATAAAATGGGGAACAGAGGAGCTTTTTAATGATTCTCCTAGCACTGATGGAAAAGATACCGATGAAAATAACAGTAACAAAGATGAGGCAGTGCCAGATGTAGAACATAAGCATAGGAAGCGGACTGGTGGCTTGGGAGACGTTTATACGGATAAGTGCACTGATAGCAGCAACAAGATTGTATGGGATGAAAGTGCAATTTCAAAGTTGCTTGACCGTTCAGACCTTCAGTCTAGTTCAACTGATATTGCTGAAGGAGAAATGGAAAATGACATGCTTGGCTCAGTAAAGGCCACCGAATGGAATGAGGAACCAGCTGAAGAACAGGGAGCTGAATCACCTCCAGGTGCAAGTGATGATACTGGTGTACAAAATACTGAAGGGAAAGAGGATAACATGGTTACTGAAGAAAATGAATGGGACAGACTTTTGCGCCTTAGGTGGGAGAAATACCAAAGCGAGGAGGAAGCAGCTCTTGGTCGTGGGAAGCGCCAACGGAAAGCGGTTTCTTACAGAGAAGCATATGCTGCACACCCAACTGAAACATTGAGTGAGGGTGCTGACGAGGAGCATGAGCCCGAACCAGAGCCAGAGAGGGAGTACACACCTGCTGGACGAGCTTTGAAAGAAAAGTTTGCTAAGCTCCGAGCTAGACAAAAAGAACGACTTGCTCAGAGGAATGCAGTTGAAGAACCTCAACCTAGTGAGGGACTACCTGTTGAGTCACTTCCTCAGGGTCCTACCAACACTGCCAAAGATGGGGATCAAGCAACTGAATTAGTTCAGTTTTTCAGGGAGAGGCCTTCTGTAATTGACTTGGAGGACGATAAGTTAGATCCCCCTAAGGCCAAGACTGATTCGCCCTTACGATTGGGCCGGCTATTGAAGCATAAAAGCAGTCACCTTGATCTTTCTGTTAATTCTCTTGACTACATGTCACCTGATATTTTCCTTCCCAGTCACCAGGTTCCGGGAACAAGCTCACTGCCCTCAAATAATTTATTACCTGTTCTGGGACTCTGTGCTCCCAATGCTAGTCAAATAGGATCATCAAATAAGAAATTTTCAAGGTCAAACGGCAGACAAAAAGGAGCTCGGCCAGAGTTTCCATTTAGTCTGGATCCTCGGTCTGGGACTGTGAATGAGACAGAAGTAAATGGTGATGACATGAAGCTATCAGATGCACCAGCTGAAGTATCACGTCTCAAGAATAAGTTGATTAACATCCCAAATGGTGGTTTTCCATTTAGGCCGTATCCTCCGCCTGGGCAAGGAAGCGGTCATGATCATCCAGAAAGTTCCGGTGCTTCCTTTTCTGATTTCCAGGAAAAGATGGCATTGCCTAACTTACCATTTGATGAGAAATTGCTACCAAGATTCCCACTTGGAGCAAAGAACATGCTGAGTCCACATCTAGACTTCTTACCTAACTTGTCATTGGGTAGCAGACTCGAATCTGCTAGTGGCTCCTTGCAAGAACTTCCAACAATGTCATTGTTTCCGAATTTAAAATTTCCGCCAGATGCAGCCAGATATAATCAGCAAGATAGAGATGTGCCTCCCTCCTTGGGTTTGGGACATATGCCAACTAATTTTCCATCTTTTCCTGATAACCACCGGAAGGTGCTCGAAAACATAATGATGAGGACTGGTCCTGGATCAAGCAACTTATTCAAAAAGAAATTTAAGGCAGATGTGTGGACGGAAGATGAACTTGATTTTCTATGGATTGGTGTTCGAAGGCATGGAAGGGGCAATTGGGATGCAATGCTGAGAGACCCGAGGTTGAAGTTCTCAAAGTTTAAAACTTCAGAAGATCTGTCAGCTAGGTGGGAGGAGGAGCAACTCAAGATTTTAGATGGGTCAGCTTTTGCAGGGTCAAAGTCAATAAAAAAGACTGCCAAATCCTCACAGTTTCCAAGCATATCTGATGGGATGATGGCACGGGCACTGCATGGAAGTAGACTTGTTACACCGCCAAAATTTCAATCCCATTTGACAGATATAAAGCTGGGTTTTACTGATCTTTCATCTGGCTTTCCGCATTTTGAATCATCTGATAGACTCGGTTTGCAGAATGAGCAATATCCCCCTATTCCATCTTGGTTTCATGATAAATTCAGGACTAATTTTTCTGAAGATTCTGCTGCCGGAGCTTCTGACAGAGCAGGGACATCTTCCAGTGTTCCCGCTGAGCAGCCATTTGTTGTCACTTCTTTTGGTACCAGTTGCTTGGGTTCGTTAGGCTTGAATTCCGCTAGCAACTATGATGTACAGAAGAAGGAAGATGAACAGGGTGCTCATAGGTACGGCAAGTTGCCTTGCGTGCTCGATAGGTCACTGAATGCTTTACGTGATATGAATAATAATTTGGGAAGAGGTGAACCCTCCAGTTCAGGATTGCTGCCTAATCTCAAGAGTGGGATTTTAAAGGGAGAAGATGTTGCTGGAAGTAGTTCTTCGAAGGACACGCTACCCCATTGGCTACGGGAAGCTGTAAGTGTTCCTGCTAAACCCCCAGTACCTGACCTGCCACCCACAGTGTCCGCAATAGCTCAATCTGTCCGGTTGTTATATGGGGAGGAGAAGCGAAGTATTCCTCCATTTGTGATTCCCGGTCCACCTCCTTCGCTACCAAAGGATCCAAGGCGAAGTCTAAAGAAGAAAAGAAAGCAGAAATCACGTCTATTTAGGCGGGTTAAACTAGACATTGCTGGAAGAGGCCAGGACTTTCATAGCAGGCACGCTGGCGACAATGCTTCAAGCTCCATTCCAATGGCCCCATCATTTCCATTGCTTTCACAAGCAATGGCTGCAACTTCAGGCCTTTCACGGATTGAATCCGGCCTAAGTGCGCCTCTGAGTATGGTAAATCCATCATCCTCAGCGCCACATCTGAATCAGCAGAAGAAAACAACCATGGGATTGTCGCCTTCCCCTGAAGTGCTTCAATTGGTAGCATCCTGCGTTGCTCCAGGTCCACATTTGTCAGCAGCTTCTGGCATGGCAAGCTCAAGCTTCCGTGACGCAAAGCCCTCATTGCCAAACTCCGTTGACCAAGTTGAACTCTTGGACTCGCAAACTGCAACTGCTATGGTAAGAACGATCTCGCCTGTTAGAACATGTGATACTGTCAGCGGGGATTCCAGCAAGACACAGTCGGATCCCCCTAGGACAGAACGACTCGATGTAGAGGAAATTTCTTCTGAGGGCACTGTCTCGGATCATCCCGTGAGTGATCAGGAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

2347

Amino Acids

258.94

Weight (kDa)

7.42

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 68 - 109 2.6e-09 PHD-finger
Chromo PF00385 694 - 745 1.9e-08 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 786 - 957 1e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 789 - 1078 7.1e-79 SNF2-related domain
HDA2-3 PF11496 1026 - 1243 7.6e-13 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 1102 - 1216 3.6e-23 Helicase conserved C-terminal domain
DUF1087 PF06465 1392 - 1436 2e-06 CHD subfamily II, DUF1087
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2054, 3791
AarI CACCTGC 2 cut(s) 1892, 4405
AasI GACNNNNNNGTC 3 cut(s) 1370, 1391, 2192
AbsI CCTCGAGG 1 cut(s) 3398
Acc36I ACCTGC 4 cut(s) 1198, 1892, 4405, 6327
Acc65I GGTACC 2 cut(s) 2864, 6011
AccB1I GGYRCC 2 cut(s) 2864, 6011
AccB7I CCANNNNNTGG 3 cut(s) 2251, 4089, 5024
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 3 cut(s) 2101, 3447, 5752
AccII CGCG 3 cut(s) 2106, 2748, 2850
AcoI YGGCCR 4 cut(s) 2004, 3300, 3411, 4886
AflII CTTAAG 2 cut(s) 287, 3329
AflIII ACRYGT 3 cut(s) 1567, 4722, 6911
AhdI GACNNNNNGTC 5 cut(s) 697, 3259, 3471, 4018, 4723
AjiI CACGTC 2 cut(s) 4991, 6473
AjnI CCWGG 9 cut(s) 103, 3238, 4132, 4752, 5053, 5112, 5443, 6513, 6763
AjuI GAANNNNNNNTTGG 6 cut(s) 4248, 4280, 5530, 5562, 6143, 6175
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 8 cut(s) 707, 3637, 3950, 4816, 4885, 5418, 6091, 6120
Alw26I GTCTC 9 cut(s) 746, 1313, 1962, 2156, 3921, 4928, 4997, 5559, 7012
Alw44I GTGCAC 1 cut(s) 3946
AlwNI CAGNNNCTG 4 cut(s) 1844, 2215, 6314, 6788
Ama87I CYCGRG 2 cut(s) 3398, 5569
ApaI GGGCCC 1 cut(s) 1884
ApaLI GTGCAC 1 cut(s) 3946
ArsI GACNNNNNNTTYG 2 cut(s) 1494, 1526
AseI ATTAAT 1 cut(s) 146
Asp700I GAANNNNTTC 4 cut(s) 2721, 4569, 6244, 6735
Asp718I GGTACC 2 cut(s) 2864, 6011
AspA2I CCTAGG 1 cut(s) 6959
AspLEI GCGC 9 cut(s) 129, 294, 921, 2244, 3705, 4224, 4281, 6646, 6679
AsuC2I CCSGG 2 cut(s) 4761, 6405
AsuII TTCGAA 2 cut(s) 5527, 6251
AsuNHI GCTAGC 1 cut(s) 6047
AvaI CYCGRG 2 cut(s) 3398, 5569
AvaII GGWCC 7 cut(s) 53, 2619, 3425, 4526, 5441, 6407, 6767
AvrII CCTAGG 1 cut(s) 6959
AxyI CCTNAGG 5 cut(s) 1110, 2337, 4226, 4521, 4632
BaeGI GKGCMC 4 cut(s) 1884, 3950, 5740, 7004
BaeI ACNNNNGTAYC 2 cut(s) 4140, 4173
BalI TGGCCA 2 cut(s) 2006, 3413
BamHI GGATCC 4 cut(s) 3764, 4909, 6430, 6952
BanI GGYRCC 2 cut(s) 2864, 6011
BanII GRGCYC 5 cut(s) 707, 1884, 2575, 4368, 4885
BbsI GAAGAC 3 cut(s) 84, 116, 1824
Bbv12I GWGCWC 8 cut(s) 707, 3637, 3950, 4816, 4885, 5418, 6091, 6120
BbvCI CCTCAGC 1 cut(s) 6673
BceAI ACGGC 4 cut(s) 3466, 4882, 5026, 6115
BciT130I CCWGG 9 cut(s) 105, 3240, 4134, 4754, 5055, 5114, 5445, 6515, 6765
BciVI GTATCC 3 cut(s) 376, 1813, 5055
BclI TGATCA 5 cut(s) 58, 340, 3739, 5074, 7027
BcnI CCSGG 2 cut(s) 4761, 6405
BcoDI GTCTC 9 cut(s) 746, 1313, 1962, 2156, 3921, 4928, 4997, 5559, 7012
BfmI CTRYAG 6 cut(s) 615, 1205, 1689, 1695, 2976, 3264
BfoI RGCGCY 3 cut(s) 2245, 4282, 6680
BfrI CTTAAG 2 cut(s) 287, 3329
BfuAI ACCTGC 4 cut(s) 1198, 1892, 4405, 6327
BfuI GTATCC 3 cut(s) 376, 1813, 5055
BglI GCCNNNNNGGC 1 cut(s) 2548
BglII AGATCT 2 cut(s) 1580, 5605
BlnI CCTAGG 1 cut(s) 6959
BlpI GCTNAGC 2 cut(s) 4428, 5982
Bme18I GGWCC 7 cut(s) 53, 2619, 3425, 4526, 5441, 6407, 6767
BmeRI GACNNNNNGTC 5 cut(s) 697, 3259, 3471, 4018, 4723
BmeT110I CYCGRG 2 cut(s) 3398, 5569
BmgBI CACGTC 2 cut(s) 4991, 6473
BmrI ACTGGG 2 cut(s) 4740, 6302
BmtI GCTAGC 1 cut(s) 6051
BmuI ACTGGG 2 cut(s) 4740, 6302
BoxI GACNNNNGTC 1 cut(s) 1293
BpiI GAAGAC 3 cut(s) 84, 116, 1824
BplI GAGNNNNNCTC 6 cut(s) 545, 577, 3080, 3112, 3239, 3271
BpmI CTGGAG 5 cut(s) 87, 251, 4116, 6163, 6747
Bpu10I CCTNAGC 2 cut(s) 1638, 6673
Bpu1102I GCTNAGC 2 cut(s) 4428, 5982
Bpu14I TTCGAA 2 cut(s) 5527, 6251
BpuEI CTTGAG 5 cut(s) 493, 4979, 5621, 6188, 6787
BpuMI CCSGG 2 cut(s) 4761, 6405
Bsa29I ATCGAT 1 cut(s) 2220
BsaAI YACGTR 3 cut(s) 1271, 2712, 6143
BsaBI GATNNNNATC 4 cut(s) 1416, 1482, 4551, 5012
BsaI GGTCTC 2 cut(s) 2156, 5559
BsaWI WCCGGW 3 cut(s) 5090, 5406, 6354
BsaXI ACNNNNNCTCC 2 cut(s) 6216, 6246
Bse118I RCCGGY 2 cut(s) 2920, 4665
Bse21I CCTNAGG 5 cut(s) 1110, 2337, 4226, 4521, 4632
Bse3DI GCAATG 8 cut(s) 320, 3129, 5126, 5562, 6498, 6581, 6603, 6827
Bse8I GATNNNNATC 4 cut(s) 1416, 1482, 4551, 5012
BseBI CCWGG 9 cut(s) 105, 3240, 4134, 4754, 5055, 5114, 5445, 6515, 6765
BseCI ATCGAT 1 cut(s) 2220
BseJI GATNNNNATC 4 cut(s) 1416, 1482, 4551, 5012
BseMI GCAATG 8 cut(s) 320, 3129, 5126, 5562, 6498, 6581, 6603, 6827
BseRI GAGGAG 6 cut(s) 3821, 4265, 4370, 5639, 5642, 6386
BseSI GKGCMC 4 cut(s) 1884, 3950, 5740, 7004
BseYI CCCAGC 1 cut(s) 5800
BsgI GTGCAG 1 cut(s) 4302
Bsh1236I CGCG 3 cut(s) 2106, 2748, 2850
BshNI GGYRCC 2 cut(s) 2864, 6011
BshVI ATCGAT 1 cut(s) 2220
BsiHKAI GWGCWC 8 cut(s) 707, 3637, 3950, 4816, 4885, 5418, 6091, 6120
BsiHKCI CYCGRG 2 cut(s) 3398, 5569
BsiSI CCGG 9 cut(s) 2921, 4666, 4760, 5091, 5407, 5940, 6355, 6405, 6633
BslFI GGGAC 9 cut(s) 234, 656, 1930, 4223, 4512, 4818, 4936, 5379, 5974
BsmAI GTCTC 9 cut(s) 746, 1313, 1962, 2156, 3921, 4928, 4997, 5559, 7012
BsmBI CGTCTC 3 cut(s) 746, 3921, 4997
BsmFI GGGAC 9 cut(s) 234, 656, 1930, 4223, 4512, 4818, 4936, 5379, 5974
BsmI GAATGC 5 cut(s) 294, 2171, 3319, 4474, 6139
Bso31I GGTCTC 2 cut(s) 2156, 5559
BsoBI CYCGRG 2 cut(s) 3398, 5569
Bsp119I TTCGAA 2 cut(s) 5527, 6251
Bsp120I GGGCCC 1 cut(s) 1880
Bsp1407I TGTACA 3 cut(s) 1572, 4156, 6061
Bsp1720I GCTNAGC 2 cut(s) 4428, 5982
Bsp19I CCATGG 4 cut(s) 535, 1030, 1614, 6708
BspDI ATCGAT 1 cut(s) 2220
BspFNI CGCG 3 cut(s) 2106, 2748, 2850
BspHI TCATGA 3 cut(s) 3277, 5071, 5899
BspMAI CTGCAG 5 cut(s) 619, 1209, 1699, 2980, 3268
BspMI ACCTGC 4 cut(s) 1198, 1892, 4405, 6327
BspOI GCTAGC 1 cut(s) 6051
BspQI GCTCTTC 1 cut(s) 3693
BspT104I TTCGAA 2 cut(s) 5527, 6251
BspT107I GGYRCC 2 cut(s) 2864, 6011
BspTI CTTAAG 2 cut(s) 287, 3329
BspTNI GGTCTC 2 cut(s) 2156, 5559
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 8 cut(s) 320, 3129, 5126, 5562, 6498, 6581, 6603, 6827
BsrFI RCCGGY 2 cut(s) 2920, 4665
BsrGI TGTACA 3 cut(s) 1572, 4156, 6061
BssAI RCCGGY 2 cut(s) 2920, 4665
Bst2UI CCWGG 9 cut(s) 105, 3240, 4134, 4754, 5055, 5114, 5445, 6515, 6765
Bst6I CTCTTC 6 cut(s) 121, 672, 2877, 3693, 6160, 6502
BstAFI CTTAAG 2 cut(s) 287, 3329
BstAPI GCANNNNNTGC 4 cut(s) 980, 3325, 4316, 6602
BstAUI TGTACA 3 cut(s) 1572, 4156, 6061
BstBAI YACGTR 3 cut(s) 1271, 2712, 6143
BstBI TTCGAA 2 cut(s) 5527, 6251
BstDSI CCRYGG 5 cut(s) 535, 1030, 1614, 2746, 6708
BstENI CCTNNNNNAGG 1 cut(s) 5058
BstFNI CGCG 3 cut(s) 2106, 2748, 2850
BstH2I RGCGCY 3 cut(s) 2245, 4282, 6680
BstHHI GCGC 9 cut(s) 129, 294, 921, 2244, 3705, 4224, 4281, 6646, 6679
BstMAI GTCTC 9 cut(s) 746, 1313, 1962, 2156, 3921, 4928, 4997, 5559, 7012
BstNI CCWGG 9 cut(s) 105, 3240, 4134, 4754, 5055, 5114, 5445, 6515, 6765
BstNSI RCATGY 8 cut(s) 1571, 1802, 2648, 3611, 4066, 4726, 5191, 6915
BstPAI GACNNNNGTC 1 cut(s) 1293
BstSFI CTRYAG 6 cut(s) 615, 1205, 1689, 1695, 2976, 3264
BstSLI GKGCMC 4 cut(s) 1884, 3950, 5740, 7004
BstSNI TACGTA 2 cut(s) 1271, 2712
BstUI CGCG 3 cut(s) 2106, 2748, 2850
BstV2I GAAGAC 3 cut(s) 84, 116, 1824
BstXI CCANNNNNNTGG 2 cut(s) 6022, 6565
Bsu15I ATCGAT 1 cut(s) 2220
Bsu36I CCTNAGG 5 cut(s) 1110, 2337, 4226, 4521, 4632
BsuI GTATCC 3 cut(s) 376, 1813, 5055
BsuTUI ATCGAT 1 cut(s) 2220
BtgI CCRYGG 5 cut(s) 535, 1030, 1614, 2746, 6708
BtgZI GCGATG 1 cut(s) 2831
BtrI CACGTC 2 cut(s) 4991, 6473
BtsI GCAGTG 4 cut(s) 3885, 4536, 4772, 5738
BveI ACCTGC 4 cut(s) 1198, 1892, 4405, 6327
CaiI CAGNNNCTG 4 cut(s) 1844, 2215, 6314, 6788
CciI TCATGA 3 cut(s) 3277, 5071, 5899
CfoI GCGC 9 cut(s) 129, 294, 921, 2244, 3705, 4224, 4281, 6646, 6679
Cfr10I RCCGGY 2 cut(s) 2920, 4665
Cfr42I CCGCGG 1 cut(s) 2749
ClaI ATCGAT 1 cut(s) 2220
CseI GACGC 2 cut(s) 89, 6824
CsiI ACCWGGT 1 cut(s) 4752
DraI TTTAAA 3 cut(s) 5298, 5593, 6219
DrdI GACNNNNNNGTC 3 cut(s) 1370, 1391, 2192
DriI GACNNNNNGTC 5 cut(s) 697, 3259, 3471, 4018, 4723
DseDI GACNNNNNNGTC 3 cut(s) 1370, 1391, 2192
EaeI YGGCCR 4 cut(s) 2004, 3300, 3411, 4886
Eam1104I CTCTTC 6 cut(s) 121, 672, 2877, 3693, 6160, 6502
Eam1105I GACNNNNNGTC 5 cut(s) 697, 3259, 3471, 4018, 4723
EarI CTCTTC 6 cut(s) 121, 672, 2877, 3693, 6160, 6502
EciI GGCGGA 2 cut(s) 5040, 5295
Ecl136II GAGCTC 2 cut(s) 705, 4883
Eco105I TACGTA 2 cut(s) 1271, 2712
Eco147I AGGCCT 2 cut(s) 4591, 6615
Eco24I GRGCYC 5 cut(s) 707, 1884, 2575, 4368, 4885
Eco31I GGTCTC 2 cut(s) 2156, 5559
Eco32I GATATC 1 cut(s) 3394
Eco47I GGWCC 7 cut(s) 53, 2619, 3425, 4526, 5441, 6407, 6767
Eco53kI GAGCTC 2 cut(s) 705, 4883
Eco81I CCTNAGG 5 cut(s) 1110, 2337, 4226, 4521, 4632
Eco88I CYCGRG 2 cut(s) 3398, 5569
EcoICRI GAGCTC 2 cut(s) 705, 4883
EcoNI CCTNNNNNAGG 1 cut(s) 5058
EcoO109I RGGNCCY 3 cut(s) 1880, 1881, 4526
EcoRI GAATTC 2 cut(s) 682, 6040
EcoRII CCWGG 9 cut(s) 103, 3238, 4132, 4752, 5053, 5112, 5443, 6513, 6763
EcoRV GATATC 1 cut(s) 3394
EcoT22I ATGCAT 1 cut(s) 1800
EcoT38I GRGCYC 5 cut(s) 707, 1884, 2575, 4368, 4885
Esp3I CGTCTC 3 cut(s) 746, 3921, 4997
FalI AAGNNNNNCTT 8 cut(s) 4248, 4280, 4440, 4472, 5487, 5519, 6232, 6264
FaqI GGGAC 9 cut(s) 234, 656, 1930, 4223, 4512, 4818, 4936, 5379, 5974
FauI CCCGC 6 cut(s) 2082, 2741, 3612, 5986, 6477, 6920
FauNDI CATATG 4 cut(s) 3018, 3436, 4312, 5370
FbaI TGATCA 5 cut(s) 58, 340, 3739, 5074, 7027
FblI GTMKAC 3 cut(s) 2101, 3447, 5752
FriOI GRGCYC 5 cut(s) 707, 1884, 2575, 4368, 4885
GlaI GCGC 9 cut(s) 128, 293, 920, 2243, 3704, 4223, 4280, 6645, 6678
GsaI CCCAGC 1 cut(s) 5804
GsuI CTGGAG 5 cut(s) 87, 251, 4116, 6163, 6747
HaeII RGCGCY 3 cut(s) 2245, 4282, 6680
HapII CCGG 9 cut(s) 2921, 4666, 4760, 5091, 5407, 5940, 6355, 6405, 6633
HgaI GACGC 2 cut(s) 89, 6824
HhaI GCGC 9 cut(s) 129, 294, 921, 2244, 3705, 4224, 4281, 6646, 6679
Hin6I GCGC 9 cut(s) 127, 292, 919, 2242, 3703, 4222, 4279, 6644, 6677
HinP1I GCGC 9 cut(s) 127, 292, 919, 2242, 3703, 4222, 4279, 6644, 6677
HincII GTYRAC 3 cut(s) 2859, 3309, 6844
HindII GTYRAC 3 cut(s) 2859, 3309, 6844
HindIII AAGCTT 3 cut(s) 41, 2810, 6805
HpaII CCGG 9 cut(s) 2921, 4666, 4760, 5091, 5407, 5940, 6355, 6405, 6633
HspAI GCGC 9 cut(s) 127, 292, 919, 2242, 3703, 4222, 4279, 6644, 6677
KpnI GGTACC 2 cut(s) 2868, 6015
KroI GCCGGC 2 cut(s) 2920, 4665
KroNI GCCGGC 2 cut(s) 2922, 4667
Ksp22I TGATCA 5 cut(s) 58, 340, 3739, 5074, 7027
KspI CCGCGG 1 cut(s) 2749
LguI GCTCTTC 1 cut(s) 3693
MabI ACCWGGT 1 cut(s) 4752
MbiI CCGCTC 1 cut(s) 157
MfeI CAATTG 3 cut(s) 3483, 5545, 6740
MlsI TGGCCA 2 cut(s) 2006, 3413
MluNI TGGCCA 2 cut(s) 2006, 3413
MmeI TCCRAC 5 cut(s) 344, 1354, 1955, 5297, 6930
Mox20I TGGCCA 2 cut(s) 2006, 3413
Mph1103I ATGCAT 1 cut(s) 1800
MroNI GCCGGC 2 cut(s) 2920, 4665
MroXI GAANNNNTTC 4 cut(s) 2721, 4569, 6244, 6735
MscI TGGCCA 2 cut(s) 2006, 3413
MslI CAYNNNNRTG 6 cut(s) 1619, 2604, 2649, 3197, 5429, 6563
Msp20I TGGCCA 2 cut(s) 2006, 3413
MspA1I CMGCKG 8 cut(s) 1280, 2089, 2748, 3569, 4106, 4979, 5981, 6927
MspCI CTTAAG 2 cut(s) 287, 3329
MspI CCGG 9 cut(s) 2921, 4666, 4760, 5091, 5407, 5940, 6355, 6405, 6633
MunI CAATTG 3 cut(s) 3483, 5545, 6740
Mva1269I GAATGC 5 cut(s) 294, 2171, 3319, 4474, 6139
MvaI CCWGG 9 cut(s) 105, 3240, 4134, 4754, 5055, 5114, 5445, 6515, 6765
MvnI CGCG 3 cut(s) 2106, 2748, 2850
NaeI GCCGGC 2 cut(s) 2922, 4667
NciI CCSGG 2 cut(s) 4761, 6405
NcoI CCATGG 4 cut(s) 535, 1030, 1614, 6708
NdeI CATATG 4 cut(s) 3018, 3436, 4312, 5370
NgoMIV GCCGGC 2 cut(s) 2920, 4665
NheI GCTAGC 1 cut(s) 6047
NmeAIII GCCGAG 1 cut(s) 4864
NsiI ATGCAT 1 cut(s) 1800
NspI RCATGY 8 cut(s) 1571, 1802, 2648, 3611, 4066, 4726, 5191, 6915
NspV TTCGAA 2 cut(s) 5527, 6251
PaeI GCATGC 2 cut(s) 2648, 3611
PaeR7I CTCGAG 1 cut(s) 3398
PagI TCATGA 3 cut(s) 3277, 5071, 5899
PaqCI CACCTGC 2 cut(s) 1892, 4405
PceI AGGCCT 2 cut(s) 4591, 6615
PciI ACATGT 3 cut(s) 1567, 4722, 6911
PciSI GCTCTTC 1 cut(s) 3693
PcsI WCGNNNNNNNCGW 1 cut(s) 1267
PctI GAATGC 5 cut(s) 294, 2171, 3319, 4474, 6139
PdiI GCCGGC 2 cut(s) 2922, 4667
PdmI GAANNNNTTC 4 cut(s) 2721, 4569, 6244, 6735
PflFI GACNNNGTC 2 cut(s) 3575, 6946
PflMI CCANNNNNTGG 3 cut(s) 2251, 4089, 5024
PfoI TCCNGGA 3 cut(s) 4759, 5112, 5443
Ppu21I YACGTR 3 cut(s) 1271, 2712, 6143
PpuMI RGGWCCY 1 cut(s) 4526
PscI ACATGT 3 cut(s) 1567, 4722, 6911
PshAI GACNNNNGTC 1 cut(s) 1293
PshBI ATTAAT 1 cut(s) 146
PsiI TTATAA 2 cut(s) 2054, 3791
Psp124BI GAGCTC 2 cut(s) 707, 4885
Psp5II RGGWCCY 1 cut(s) 4526
Psp6I CCWGG 9 cut(s) 103, 3238, 4132, 4752, 5053, 5112, 5443, 6513, 6763
PspFI CCCAGC 1 cut(s) 5800
PspGI CCWGG 9 cut(s) 103, 3238, 4132, 4752, 5053, 5112, 5443, 6513, 6763
PspOMI GGGCCC 1 cut(s) 1880
PspPPI RGGWCCY 1 cut(s) 4526
PspXI VCTCGAGB 1 cut(s) 3398
PsrI GAACNNNNNNTAC 2 cut(s) 6901, 6933
PstI CTGCAG 5 cut(s) 619, 1209, 1699, 2980, 3268
PstNI CAGNNNCTG 4 cut(s) 1844, 2215, 6314, 6788
PsyI GACNNNGTC 2 cut(s) 3575, 6946
PvuII CAGCTG 4 cut(s) 1280, 3569, 4106, 4979
RseI CAYNNNNRTG 6 cut(s) 1619, 2604, 2649, 3197, 5429, 6563
SacI GAGCTC 2 cut(s) 707, 4885
SacII CCGCGG 1 cut(s) 2749
SapI GCTCTTC 1 cut(s) 3693
SexAI ACCWGGT 1 cut(s) 4752
SfcI CTRYAG 6 cut(s) 615, 1205, 1689, 1695, 2976, 3264
Sfr274I CTCGAG 1 cut(s) 3398
Sfr303I CCGCGG 1 cut(s) 2749
SfuI TTCGAA 2 cut(s) 5527, 6251
SgrBI CCGCGG 1 cut(s) 2749
SinI GGWCC 7 cut(s) 53, 2619, 3425, 4526, 5441, 6407, 6767
SlaI CTCGAG 1 cut(s) 3398
SmiMI CAYNNNNRTG 6 cut(s) 1619, 2604, 2649, 3197, 5429, 6563
SmlI CTYRAG 8 cut(s) 287, 508, 3329, 3398, 4994, 5636, 6203, 6802
SmoI CTYRAG 8 cut(s) 287, 508, 3329, 3398, 4994, 5636, 6203, 6802
SnaBI TACGTA 2 cut(s) 1271, 2712
SphI GCATGC 2 cut(s) 2648, 3611
SseBI AGGCCT 2 cut(s) 4591, 6615
SspI AATATT 1 cut(s) 3157
SstI GAGCTC 2 cut(s) 707, 4885
StuI AGGCCT 2 cut(s) 4591, 6615
TaqII GACCGA 1 cut(s) 4905
TatI WGTACW 5 cut(s) 812, 1572, 4156, 4389, 6061
TauI GCSGC 1 cut(s) 3546
Tth111I GACNNNGTC 2 cut(s) 3575, 6946
Van91I CCANNNNNTGG 3 cut(s) 2251, 4089, 5024
Vha464I CTTAAG 2 cut(s) 287, 3329
VneI GTGCAC 1 cut(s) 3946
VpaK11BI GGWCC 7 cut(s) 53, 2619, 3425, 4526, 5441, 6407, 6767
VspI ATTAAT 1 cut(s) 146
XagI CCTNNNNNAGG 1 cut(s) 5058
XbaI TCTAGA 1 cut(s) 5203
XceI RCATGY 8 cut(s) 1571, 1802, 2648, 3611, 4066, 4726, 5191, 6915
XhoI CTCGAG 1 cut(s) 3398
XmaJI CCTAGG 1 cut(s) 6959
XmiI GTMKAC 3 cut(s) 2101, 3447, 5752
XmnI GAANNNNTTC 4 cut(s) 2721, 4569, 6244, 6735
Zsp2I ATGCAT 1 cut(s) 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.