Rroxscaffold_1G00057710

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005784
Physical Location & Seq
Reverse (-)
79702437 .. 79715893
13457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057710.1

Sequence Viewer

Length: 7281 bp
ATGAAGGATAACAGCTCGTCAACAAGTACAATGATTAACAGAAACTGGGTCTTGAAACGAAAACGTAGAAAGCTTCCTTATGGACCTGCTCTATCTAATGGCAAAGAAGACAGTTCAGCAGCATCAGAATCTCAAGGGAAGACATCTTCTTCCACTAAACGACGGCTGACTAATGAAATTATTTCTGATCGACTTTCGTCCAAGAAGAAAGGAAACGATGGGTATTACTATGAATGTGTGGTCTGTGACCTTGGTGGTAACTTGTTGTGTTGTGATAGTTGTCCCCAAACCTATCATCTCCAGTGTCTTAATCCACCTCTTAAGCGGATTCCGAATGGGAAGTGGCAGTGCCCAAACTGCTGTCAGAAAAGTGATATGCCAGAGCCTTTAAAGTATCTAGCTGATAACATTTCAAAACGAGCAAGGACAAAGATTGCCACCTCGAAGTCCAAAGCTGAAGTTAAGTCATCTGACAGAGAGAAAGTTTCGCAGATTTTTGGGAACACCATTGTTGCAAAGAAAAGATCATCAAGCAAAGGGAAAGCTCATGGAGTTAAATCCTTCGAAAAGAAGCCATTCTCCCAGATAGATACATCTTCTAGCATGAAGCCCAGTCATTCATCAGCTGGCGGTTCTGTGGATGGTATTTCATTGTCTGAGAATGTTGGGGATGAAAATAGATCTAATCTTTCCCCACCAGATGATTCTGCAGACAAAAAGTTGAGCTCTCCAGCTAAAGAGGTTTTATCTCATTCTAAAATATCAGAGACAAATGAAGAAGCTCCTGACGCATCTATGACAAATGAAGAAGCTCCGGAGGCAGCTGTGACAACTGGAGAAGCTCTGGAGGCAGCTGTGACAACTGGAGAAGCTCCGGAGGCAGCTGTGACAACTGGAGAAGCTCCGGAGGCAGCTGTGACAACTGGAGAAGCTCCGGAGGCAGCTGTGACAACTGTAGAAGCTCCTGAGGCATCTGTGCCAAATGAAGAAGCTCCTGAGGCATCTGTGACAAATGGAGAAGCTCCTGAGCCATCTGTCCTTCCTGAAGTGAAGCCTGACCCGTCTTGTGATGCATCTCCGCGTAAGACCATTGTTCTTGCCATCAGTGCTGCTGCTGGCAAGGCTAGAAAAAGAAAACACAAAGGCAATAATGAGAAGAGTAAAAAGAAGCGCAGGACAGAGAAGAGGAAAACTGTCATCGGTATTTCTAAACAGTCTGGATCTAAAGCAAATACCTCAAGCCCAGGGACTCGTATTCGTAAAACACTTCGTAAGCATAAATCTCTCAACCATGGGGTTTCTGCATCTTTGTCGAGGGAGGATGTCACAACCAAGGGTTCAGATGTCCAGATGAAAGACGAGGACCTTACTGAGGAAGCAAAAGACCAATTTCATAATGCAGATAAAGCCGGAAATCATGGAGTCGAAACATTAATGCAGAAGGATAGTCTTACTGCTGAACTCCTGCAGGTGGATCGGGTTTTGGGATGTCGAGTTCAAGGTAGTCATGCTGCTGCTTCTTGTCACTTATCAGTGACAGCTGCCCAGGATCTGAGTTCTGATGATGCACAAGTCTCAGAAAATAATAACAGACTATCACAGGAAAATTTTGCTTGCGAAACTGTTATGGATGTTGGGGTGGCTAAAAATCTCACTGAGGGTTGTCAAGGAGTTGTTAAGGATGTTGATGGGGATGACAGCAAGAAGGAGGATATAAGAGTGGACAAACTGCATGTGTACAGAAGGTCTATGAACAAAGAAGGTAAAAGAGCTAATTCCATGGATTTGTCTAGGAAAGATACCAAGGAGTTGGATCCTGCAGGCATAATTGATCACGATCCAAACGAATCTGCTTTAAATGCTGATGATCCAGAAAAAACAAATGAAGAAGTAGTGACTGCAGAGAATATTGATGTCAATTTGAACAGTCATGATAAAGATAAAGAAGAGGTTCTGGAAATTTGTGAAGTGCATGTTTCTGCTGATACCAATGACAAGAGAGATGTCAATACAGAAGCTGGAACTGATATCTGTGCTGAAAACAAAAGCGAAGAGCCCACACTGGCTGAACGTGCAGTTGATGGTGTTGAGACGGTATCGTATGAATTTTTAGTTAAGTGGGTAGGTAAGTCTCATATTCATAATAGCTGGGTTTCTGAATCTGAGTTAAAAGTCCTGGCGAAGAGGAAACTAGAAAACTATAAGGCAAAGTATGGAATGGCTGTTTTAAATATATGCGAGGAGAGATGGAAGCAACCACAGCGGGTGATCGCTCTCCGTGGATTTAAAGATGGTTCAGGTGAAGCTTTTGTGAAATGGACTGGTTTGCCTTATGATGAATGCACTTGGGAAAGATTGGATGAGCCTGTTATGAGAAATTCTCAGAATCTGGTTGAATTATTTAGTCAGTTTGAGCACCAAACGTTGGTAAATGATGCTCTTAAAGATGATTCATCAAGAGGGAAGGTTAGCCGACAGCAAAATGAAATACTTGCTCTGACAGAGCAGCCCAAGGAACTAAAGGGAGGTTCATTGTTTCCCCATCAGCTTGAAGCACTCAATTGGTTGCGGAAGTGCTGGCATAAATCAAGAAATGTGATATTAGCTGATGAGATGGGGCTCGGTAAAACCATATCAGCTTGTGCTTTTATTTCGTCATTATATTTTGAGTTCAAAGCCACTCTACCTTGTTTAGTCTTGGTTCCACTTTCCACAATGCCTAATTGGCTTGCTGAGTTTTCATTATGGGCCCCTGAATTGAATGTCGTGGAATACCATGGGTGTGCCAAAGCAAGAGCCATGATTCGCCAGTGTGAATGGCATGCTAGTGTTCCAAATGAGTTGAACAAGAAGACATCCGCTTATAAATTTAATGTTCTTTTAACTACATATGAAATGGTTCTTGCTGATTCCACACATCTTCGTGGGGTCCCTTGGGAAGTTCTCATAGTTGATGAGGGCCATCGATTGAAAAATTCAGGAAGTAGACTTTTCAGCTTGCTTAACTCGTTCTCTTTCCAACATCGTGTACTGTTGACTGGCACCCCTCTTCAGAATAACCTTGGTGAGATGTACAACTTGCTTAATTTCTTGCAGCCTGCATCATTCCCTTCCCTGTCTTCATTTGAGGAGAGGTTTAATGATCTTACAACTTCAGAAAAAGTGGAGGAATTGAAAAAACTCGTTGCTCCACATATGCTTCGTAGGCTTAAAAAGGATGCTATGCAGAATATTCCCCCAAAGACCGAACGAATGGTTCCTGTTGAGTTGTCCTCCATCCAAGCTGAATACTATCGTGCAATGCTAACAAAGAATTATCAGATATTGCGGAATATTGGGAAAGGGGTTGCACAACAATCGATGCTGAACATTGTAATGCAGTTAAGGAAAGTCTGCAATCATCCATATCTGATACCAGGCACTGAGCCTGACTGTGGGTCTGTAGAGTTCCTTCATGACATGCGGATAAAAGCTTCAGCCAAGTTGACACTGTTGCATTCTATGCTTAAGATTTTGCACAAAGAGGGTCACAGAGTCCTTATCTTTTCCCAGATGACCAAGCTCCTTGATATCCTTGAGGATTATTTGGCCATCGAATTTGGACCTAAAACATATGAGAGAGTAGATGGATCTGTTGCAGTGGCTGATCGTCAATCTGCAATTGCGCGTTTTAACCAAGACAGAAGTCGATTTGTCTTTTTGTTATCAACACGCTCTTGTGGCCTTGGGATCAATTTGGCAACTGCTGACACCGTCATTATTTATGATTCTGATTTCAACCCCCATGCTGATATTCAAGCTATGAATCGAGCACATCGAATCGGACAATCAAATAGACTTCTGGTTTATAGGCTTGTAGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAGAAGAAACTTATGCTGGATCAGCTATTTGTGAATAAGTCAGAATCCCAAAAAGAGGTGGAAGATATTCTTAAGTGGGGAACAGAGGAACTTTTCAATGATTCTCCTGGCATGGATGGAAAAGATACAGGTGAAAATAATAGTAACAAAGATGAGGCAGTGCCAGATGTAGAGCATAAGCATAAGAAGAGGATTGGTAGCCTGGGAGATGTTTATGAAGATAAATGTACGGAAAACAGCAACAAGATTGTTTGGGATGAAACTGCGATTTTAAAGTTGCTTGACCGTGAAAATCTTCAATCTGGACTGACTGATAATGCGGAAGGAGATATGGAAAATGATATGCTTGGCTCTGTAAAGTCTATCGAATGGAATGAGGAGCCAATTGAAGAGCAGGGAGTTGAATCACCTCCTGGTGCAAGTGATGATATATGTGCTCAAAATACTGAAAGGAAAGAGGATAATGTGGTTAATGGTACAGAAGAAAATGAATGGGACAGACTCTTACGTCTTAGATGGGAGAAATATCAAAGTGAGGAGGAAGCAGCTCTTGGTCGTGGTAAGCGAATGCGGAAAGCTGTTTCTTACAGGGAAGCATATGCTGCACACCCAAGTGAAACATTAACTGAGCAGAGTGGTGGTGGTGAAGATGAGCGTGAGCCCGAACCAGAGCCAGAGCGGGAGTACACACCCGCAGGACGAGCTCTAAAAGCAAAGTATGCTAAGCTCCGTGCTAGGCAAAAAGAACGTCTAGCTCAGAGGAATGAAATTGAAGAACCTCGTCCTAGTGAGGGACTGCCTGTTGAGTCACATCCTCAGTGTCCCATGAACACTGCCGAAGATGTTGATCAAGCGACTGGGGATCAAGCGGCTGGATTAGTTCAATTTCTCAGTGAGAAGTCTTCAGTAATTGACTTGGAGGATAACAAATTAGATGCTTCTAAAGCCAAGACTGACTCTCCCTTAAGATTGGGCAAGCTATCAAAGCATAAAAGCAGTCGCCTCGATCTTTCTGTTAATCCACTTGACTACGCGTCTCCTGACATTTTATTTCCTAGACACCAACATCAGGGAACAAGCATGACTCATTCGATGCCCCCAAATAATTTGTTACCTGTTCTGGGACTGTGTGCCCCAAATGCTAGTCAAGTAGAATCATCAAAAAAGAACTCACGGTCGAATGGCAGACGGAGAGGAGCTGGACCAGAATTTCCATTTAGTCTGGCTCCTCATTCTGGGACCTTGCCTGAAACAGAAGCAAATGGTGATGAAGCAAAGCTATCAGATGCATCCGCAGAGGCATCACAACGTTTGAAGAGTAGCATCCCAAATGGTGGCTTGCCATTTAGACCGTATCCTCCGGCTTTTCAAGGAAAAGGTTATGATCGCCCTGAAAGTTCTGGTGCCACCTTTTCTGATTTCCAAGAAAAGATGGCACTGCCCAACTTACCATTTGATGAGAAATTGCTATCAAGATTCCCGCTTTCATCTAAGAGCATACAAAGTCCACATCTTGACTTCTTACCTAACTTGTCCTTGGGTAGCAGACTCGAAACTGTCAATGGCTCCTTGCAAGAGCTTCCAACAATGCCACTGTTTCCCAATTTAAAATTGCCCCCACAAGATGCGCCCAGATATAATCAGCTAGACAGAGAAGTCCATCCCACCTTGGGTTTGGGCCATATGCCAACTACATTTCCTTCATTATCTGATAACCACAGAAAGGTGCTGGAAAACATAATGATGAGGACTGGTTCTGCATCAAACAACTTGTTTAAGAGGAAATCAAAAGCAGATAGCTGGTCGGAAGATGAACTTGATTTCCTCTGGGTTGGTGTCCGTAGGCATGGAAGGGGCAATTGGGATGCAATGCTAAGAGACCCAAGGTTGAAATTCTCAAAGTTCAAAACTTCAGAAGATTTGTCAGCAAGGTGGGAGGAGGAGCAACTCAAGCTTTTAGAAGGGTCAGCTTTTCCACTGTCAAAATCATCCAGAAAGACTCCCAAGTCCTCGCAGTTTCCAAGCATATCTGATGGAATGATGGCCAGGGCACTGCATGGAAGTCGACTTGTTACACCTCCAAAGTTTCAATCCCATTTGACGGACATGAAGTTGGGTTTTACTGATCTTACATCTGGCTTTCCACATTTTGAAGCATCGGATAGACTTGGTTTGCAAAATGAGCAATGCCCTCCAATTCCAACTTGGTTTCATGACAAATTCCGGGGGAATTTTGCCAGAGATTCTGCTGCTGGACCTTCAGACAGACCAGGGACATCTTCCAATGTTTCCATGGAGCCGCCATTTGTTGTCACTTCTTTTGGAAGCAGTTGCTTGGGTTCATTAGGTTTGAATCCCCCAAGCAGCTATGATTTGCAGCAGAAGGAAAATGAACAGGGTCCCTATAACTATGGGAAGTTGCCCAGTCTTCTGGATAAATCACTGAATGCTCTGCGTGATATGAATAATAATGTTGCAAGAGGTGAACCCAGTGCAGGATTCTTTCCTGATCCCAGGAGAGGTTTTTTAAAGGGTGAAGATGTTGCTGGAAGTAGTTCATCAAAGGACAAGCTACCTCATTGGCTACGGCAAGCTGTAAGTGCTCCTGCCAAACCCCCAGAACCTGACCTTCCCCCTACAGTATCGGCAATAGCTCGGTCAGTTCGATTGTTATATAGGGAGGAAGAGCCAACCATTCCTCCCTTTGTGATTCCAGGCCCACCTCCTTCACTACCAAAGGATCCAAGACGACGGCTGAAGAAGAAAAGGAAACAGAAGTTACATATCTTTAGGCAAATTTCCCAAGACATTGCCGGAAGCAGCCACTTAAGTGAAAATGCTTCAAGCTCCATTCCTGTGGCCCCATCATTTCCCTTGCTTTCACAATCAATGGCACCACCTCCAGGCTTTCCACCAATGGAATCTGACCTCAATATGCCTCGGAGTTTAAATATGTTAAACCCATTAGCCTCGTTGCCACATCTGAATCAACAGATCAAAACAACCATGGGGCTGTCTCCTTCCCGTGAAGTGCTTCAACTGGTAGCATCCTGTGTTGCTCCAGGTCCACATCTACCAGCAGTTTCTGGCATGACAAGCTCAAGTGTCCCTGACTTGAAGCCATCATTGCCAGATTCAGCTGGCCAAGTAGGACTCTTGGACTCGCAAGCTACATTGGCAAAAGATGAGGCAAGAGATGAGGCTAAGCCGGGTTCACCTCTTAAGGAGTGCAATTCACTCCCAAAAGACAGAAAATGTGATACGGAAAGTGGGGATTCCAGCAAGACTCAGTCAGATCCCAATAGAACTGAGCGTCCCGATGCTGAGGAAGTATCTTCTGAGGGCACCGTCTCAGATCACCCTCTGAGTGACCGGGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

2426

Amino Acids

268.28

Weight (kDa)

6.22

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 79 - 120 4.2e-09 PHD-finger
Chromo PF00385 752 - 802 2.4e-08 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 845 - 1016 6.1e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 848 - 1137 4.3e-79 SNF2-related domain
HDA2-3 PF11496 1085 - 1302 1.5e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 1161 - 1275 1.4e-22 Helicase conserved C-terminal domain
DUF1087 PF06465 1456 - 1497 2.4e-06 CHD subfamily II, DUF1087
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2865
AarI CACCTGC 1 cut(s) 1459
AasI GACNNNNNNGTC 3 cut(s) 4404, 5552, 5902
Acc36I ACCTGC 2 cut(s) 94, 1459
AccB1I GGYRCC 4 cut(s) 3041, 5300, 6790, 7241
AccB7I CCANNNNNTGG 2 cut(s) 2425, 5231
AccBSI CCGCTC 1 cut(s) 4576
AccI GTMKAC 2 cut(s) 2986, 5962
AccII CGCG 3 cut(s) 1081, 3667, 4931
AccIII TCCGGA 4 cut(s) 814, 874, 904, 934
AclI AACGTT 2 cut(s) 2423, 5206
AcoI YGGCCR 3 cut(s) 3588, 5940, 7039
AcuI CTGAAG 9 cut(s) 477, 1065, 3035, 3138, 3459, 4785, 5793, 6141, 6674
AfaI GTAC 7 cut(s) 28, 1739, 3030, 3074, 4126, 4375, 4583
AflII CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
AflIII ACRYGT 1 cut(s) 4929
AhdI GACNNNNNGTC 2 cut(s) 3436, 4930
AjuI GAANNNNNNNTTGG 4 cut(s) 4431, 4463, 5740, 5772
AleI CACNNNNGTG 2 cut(s) 4509, 6726
AloI GAACNNNNNNTCC 6 cut(s) 1479, 1511, 5703, 5735, 7195, 7227
Alw21I GWGCWC 6 cut(s) 728, 2418, 3814, 4336, 4603, 6502
Alw26I GTCTC 8 cut(s) 761, 1579, 2084, 2136, 4938, 5769, 6918, 7252
AlwNI CAGNNNCTG 8 cut(s) 45, 1552, 2018, 2389, 3422, 3644, 6521, 6983
Aor13HI TCCGGA 4 cut(s) 814, 874, 904, 934
AoxI GGCC 9 cut(s) 2748, 2959, 3588, 3721, 5575, 5940, 6613, 6756, 7039
ApaI GGGCCC 1 cut(s) 2752
ArsI GACNNNNNNTTYG 2 cut(s) 4630, 4662
AseI ATTAAT 1 cut(s) 1433
Asp700I GAANNNNTTC 7 cut(s) 575, 1950, 2898, 3963, 4191, 6451, 6930
AspLEI GCGC 4 cut(s) 1173, 3667, 3882, 5527
AsuC2I CCSGG 3 cut(s) 6122, 7107, 7271
AsuII TTCGAA 1 cut(s) 564
AvaII GGWCC 9 cut(s) 83, 1363, 2929, 3602, 5099, 5136, 6152, 6296, 6962
AxyI CCTNAGG 2 cut(s) 966, 996
BaeGI GKGCMC 5 cut(s) 353, 2752, 5032, 5950, 7244
BalI TGGCCA 3 cut(s) 3590, 5942, 7041
BamHI GGATCC 2 cut(s) 1813, 6637
BanI GGYRCC 4 cut(s) 3041, 5300, 6790, 7241
BanII GRGCYC 6 cut(s) 728, 2058, 2622, 2752, 4560, 4603
BarI GAAGNNNNNNTAC 2 cut(s) 3435, 3467
BbsI GAAGAC 6 cut(s) 114, 146, 2858, 3111, 4791, 6317
Bbv12I GWGCWC 6 cut(s) 728, 2418, 3814, 4336, 4603, 6502
BbvCI CCTCAGC 1 cut(s) 7221
BceAI ACGGC 3 cut(s) 179, 6500, 6665
BciVI GTATCC 1 cut(s) 5262
BclI TGATCA 2 cut(s) 1831, 4744
BcnI CCSGG 3 cut(s) 6122, 7107, 7271
BcoDI GTCTC 8 cut(s) 761, 1579, 2084, 2136, 4938, 5769, 6918, 7252
BfmI CTRYAG 7 cut(s) 708, 954, 1466, 1818, 1899, 3441, 6534
BfrI CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
BfuAI ACCTGC 2 cut(s) 94, 1459
BfuI GTATCC 1 cut(s) 5262
BglI GCCNNNNNGGC 1 cut(s) 2725
BglII AGATCT 1 cut(s) 680
BlpI GCTNAGC 2 cut(s) 4620, 7101
Bme18I GGWCC 9 cut(s) 83, 1363, 2929, 3602, 5099, 5136, 6152, 6296, 6962
BmeRI GACNNNNNGTC 2 cut(s) 3436, 4930
BmrI ACTGGG 5 cut(s) 55, 606, 4764, 6315, 6381
BmuI ACTGGG 5 cut(s) 55, 606, 4764, 6315, 6381
BoxI GACNNNNGTC 2 cut(s) 196, 3684
BpiI GAAGAC 6 cut(s) 114, 146, 2858, 3111, 4791, 6317
BplI GAGNNNNNCTC 4 cut(s) 2365, 2397, 3257, 3289
BpmI CTGGAG 9 cut(s) 284, 714, 855, 866, 885, 915, 945, 6783, 6942
Bpu10I CCTNAGC 2 cut(s) 1026, 7221
Bpu1102I GCTNAGC 2 cut(s) 4620, 7101
Bpu14I TTCGAA 1 cut(s) 564
BpuEI CTTGAG 5 cut(s) 117, 1222, 3596, 5831, 6982
BpuMI CCSGG 3 cut(s) 6122, 7107, 7271
Bsa29I ATCGAT 2 cut(s) 2965, 3359
BsaBI GATNNNNATC 2 cut(s) 1836, 4743
BsaI GGTCTC 1 cut(s) 5769
BsaWI WCCGGW 4 cut(s) 814, 874, 904, 934
BsaXI ACNNNNNCTCC 4 cut(s) 4840, 4870, 6580, 6610
Bse21I CCTNAGG 2 cut(s) 966, 996
Bse3DI GCAATG 5 cut(s) 3306, 5772, 6089, 6705, 7022
Bse8I GATNNNNATC 2 cut(s) 1836, 4743
BseAI TCCGGA 4 cut(s) 814, 874, 904, 934
BseCI ATCGAT 2 cut(s) 2965, 3359
BseJI GATNNNNATC 2 cut(s) 1836, 4743
BseMI GCAATG 5 cut(s) 3306, 5772, 6089, 6705, 7022
BseRI GAGGAG 8 cut(s) 2255, 3143, 4289, 4448, 5106, 5115, 5849, 5852
BseSI GKGCMC 5 cut(s) 353, 2752, 5032, 5950, 7244
BseYI CCCAGC 1 cut(s) 2148
BsgI GTGCAG 3 cut(s) 2094, 4485, 6411
Bsh1236I CGCG 3 cut(s) 1081, 3667, 4931
Bsh1285I CGRYCG 1 cut(s) 5075
BshFI GGCC 9 cut(s) 2750, 2961, 3590, 3723, 5577, 5942, 6615, 6758, 7041
BshNI GGYRCC 4 cut(s) 3041, 5300, 6790, 7241
BshVI ATCGAT 2 cut(s) 2965, 3359
BsiEI CGRYCG 1 cut(s) 5075
BsiHKAI GWGCWC 6 cut(s) 728, 2418, 3814, 4336, 4603, 6502
BsmAI GTCTC 8 cut(s) 761, 1579, 2084, 2136, 4938, 5769, 6918, 7252
BsmBI CGTCTC 3 cut(s) 2084, 4938, 7252
BsmI GAATGC 4 cut(s) 2345, 3496, 4470, 6349
BsnI GGCC 9 cut(s) 2750, 2961, 3590, 3723, 5577, 5942, 6615, 6758, 7041
Bso31I GGTCTC 1 cut(s) 5769
Bsp119I TTCGAA 1 cut(s) 564
Bsp120I GGGCCC 1 cut(s) 2748
Bsp13I TCCGGA 4 cut(s) 814, 874, 904, 934
Bsp1407I TGTACA 2 cut(s) 1737, 3072
Bsp1720I GCTNAGC 2 cut(s) 4620, 7101
Bsp19I CCATGG 5 cut(s) 1291, 1779, 2776, 6189, 6903
BspANI GGCC 9 cut(s) 2750, 2961, 3590, 3723, 5577, 5942, 6615, 6758, 7041
BspDI ATCGAT 2 cut(s) 2965, 3359
BspEI TCCGGA 4 cut(s) 814, 874, 904, 934
BspFNI CGCG 3 cut(s) 1081, 3667, 4931
BspHI TCATGA 3 cut(s) 1930, 3454, 6109
BspMAI CTGCAG 4 cut(s) 712, 1470, 1822, 1903
BspMI ACCTGC 2 cut(s) 94, 1459
BspQI GCTCTTC 4 cut(s) 2046, 3870, 4281, 6576
BspT104I TTCGAA 1 cut(s) 564
BspT107I GGYRCC 4 cut(s) 3041, 5300, 6790, 7241
BspTI CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
BspTNI GGTCTC 1 cut(s) 5769
BsrBI CCGCTC 1 cut(s) 4576
BsrDI GCAATG 5 cut(s) 3306, 5772, 6089, 6705, 7022
BsrGI TGTACA 2 cut(s) 1737, 3072
BstAFI CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
BstAPI GCANNNNNTGC 3 cut(s) 3502, 4499, 4616
BstAUI TGTACA 2 cut(s) 1737, 3072
BstBI TTCGAA 1 cut(s) 564
BstDSI CCRYGG 6 cut(s) 1291, 1779, 2278, 2776, 6189, 6903
BstENI CCTNNNNNAGG 1 cut(s) 1370
BstFNI CGCG 3 cut(s) 1081, 3667, 4931
BstHHI GCGC 4 cut(s) 1173, 3667, 3882, 5527
BstMAI GTCTC 8 cut(s) 761, 1579, 2084, 2136, 4938, 5769, 6918, 7252
BstMCI CGRYCG 1 cut(s) 5075
BstNSI RCATGY 4 cut(s) 1736, 1976, 2825, 3463
BstPAI GACNNNNGTC 2 cut(s) 196, 3684
BstSFI CTRYAG 7 cut(s) 708, 954, 1466, 1818, 1899, 3441, 6534
BstSLI GKGCMC 5 cut(s) 353, 2752, 5032, 5950, 7244
BstUI CGCG 3 cut(s) 1081, 3667, 4931
BstV2I GAAGAC 6 cut(s) 114, 146, 2858, 3111, 4791, 6317
BstX2I RGATCY 7 cut(s) 680, 1220, 1549, 1813, 3629, 6637, 7192
BstXI CCANNNNNNTGG 3 cut(s) 1810, 6328, 6754
BstYI RGATCY 7 cut(s) 680, 1220, 1549, 1813, 3629, 6637, 7192
Bsu15I ATCGAT 2 cut(s) 2965, 3359
Bsu36I CCTNAGG 2 cut(s) 966, 996
BsuI GTATCC 1 cut(s) 5262
BsuRI GGCC 9 cut(s) 2750, 2961, 3590, 3723, 5577, 5942, 6615, 6758, 7041
BsuTUI ATCGAT 2 cut(s) 2965, 3359
BtgI CCRYGG 6 cut(s) 1291, 1779, 2278, 2776, 6189, 6903
BtsI GCAGTG 6 cut(s) 353, 3645, 4062, 4728, 5333, 5948
BveI ACCTGC 2 cut(s) 94, 1459
CaiI CAGNNNCTG 8 cut(s) 45, 1552, 2018, 2389, 3422, 3644, 6521, 6983
CciI TCATGA 3 cut(s) 1930, 3454, 6109
CfoI GCGC 4 cut(s) 1173, 3667, 3882, 5527
ClaI ATCGAT 2 cut(s) 2965, 3359
CseI GACGC 3 cut(s) 797, 4920, 7199
Csp6I GTAC 7 cut(s) 27, 1738, 3029, 3073, 4125, 4374, 4582
CviQI GTAC 7 cut(s) 27, 1738, 3029, 3073, 4125, 4374, 4582
DraI TTTAAA 8 cut(s) 390, 1857, 2229, 2287, 4170, 5505, 6426, 6846
DrdI GACNNNNNNGTC 3 cut(s) 4404, 5552, 5902
DriI GACNNNNNGTC 2 cut(s) 3436, 4930
DseDI GACNNNNNNGTC 3 cut(s) 4404, 5552, 5902
EaeI YGGCCR 3 cut(s) 3588, 5940, 7039
Eam1105I GACNNNNNGTC 2 cut(s) 3436, 4930
Ecl136II GAGCTC 2 cut(s) 726, 4601
Eco24I GRGCYC 6 cut(s) 728, 2058, 2622, 2752, 4560, 4603
Eco31I GGTCTC 1 cut(s) 5769
Eco32I GATATC 2 cut(s) 2029, 3571
Eco47I GGWCC 9 cut(s) 83, 1363, 2929, 3602, 5099, 5136, 6152, 6296, 6962
Eco53kI GAGCTC 2 cut(s) 726, 4601
Eco57I CTGAAG 9 cut(s) 477, 1065, 3035, 3138, 3459, 4785, 5793, 6141, 6674
Eco81I CCTNAGG 2 cut(s) 966, 996
EcoICRI GAGCTC 2 cut(s) 726, 4601
EcoNI CCTNNNNNAGG 1 cut(s) 1370
EcoO109I RGGNCCY 5 cut(s) 1363, 2749, 2929, 5136, 6296
EcoRV GATATC 2 cut(s) 2029, 3571
EcoT22I ATGCAT 2 cut(s) 1075, 5188
EcoT38I GRGCYC 6 cut(s) 728, 2058, 2622, 2752, 4560, 4603
Esp3I CGTCTC 3 cut(s) 2084, 4938, 7252
FalI AAGNNNNNCTT 6 cut(s) 3951, 3983, 4431, 4463, 5697, 5729
FauI CCCGC 4 cut(s) 2256, 4569, 4597, 5385
FauNDI CATATG 5 cut(s) 2890, 3195, 3613, 4495, 5580
FbaI TGATCA 2 cut(s) 1831, 4744
FblI GTMKAC 2 cut(s) 2986, 5962
FriOI GRGCYC 6 cut(s) 728, 2058, 2622, 2752, 4560, 4603
GlaI GCGC 4 cut(s) 1172, 3666, 3881, 5526
GsaI CCCAGC 1 cut(s) 2152
GsuI CTGGAG 9 cut(s) 284, 714, 855, 866, 885, 915, 945, 6783, 6942
HaeIII GGCC 9 cut(s) 2750, 2961, 3590, 3723, 5577, 5942, 6615, 6758, 7041
HgaI GACGC 3 cut(s) 797, 4920, 7199
HhaI GCGC 4 cut(s) 1173, 3667, 3882, 5527
Hin6I GCGC 4 cut(s) 1171, 3665, 3880, 5525
HinP1I GCGC 4 cut(s) 1171, 3665, 3880, 5525
HincII GTYRAC 4 cut(s) 21, 3036, 3486, 5963
HindII GTYRAC 4 cut(s) 21, 3036, 3486, 5963
HindIII AAGCTT 4 cut(s) 71, 2304, 3471, 5849
Hpy99I CGWCG 2 cut(s) 165, 6651
HpyCH4IV ACGT 6 cut(s) 64, 2071, 2423, 4405, 4645, 5206
HpySE526I ACGT 6 cut(s) 64, 2071, 2423, 4405, 4645, 5206
HspAI GCGC 4 cut(s) 1171, 3665, 3880, 5525
KflI GGGWCCC 2 cut(s) 2929, 6296
Kpn2I TCCGGA 4 cut(s) 814, 874, 904, 934
Ksp22I TGATCA 2 cut(s) 1831, 4744
LguI GCTCTTC 4 cut(s) 2046, 3870, 4281, 6576
MaeII ACGT 6 cut(s) 64, 2071, 2423, 4405, 4645, 5206
MbiI CCGCTC 1 cut(s) 4576
MfeI CAATTG 4 cut(s) 2560, 3660, 4281, 5755
MflI RGATCY 7 cut(s) 680, 1220, 1549, 1813, 3629, 6637, 7192
MlsI TGGCCA 3 cut(s) 3590, 5942, 7041
MluI ACGCGT 1 cut(s) 4929
MluNI TGGCCA 3 cut(s) 3590, 5942, 7041
MmeI TCCRAC 5 cut(s) 1791, 3043, 5504, 5682, 6122
Mox20I TGGCCA 3 cut(s) 3590, 5942, 7041
Mph1103I ATGCAT 2 cut(s) 1075, 5188
MroI TCCGGA 4 cut(s) 814, 874, 904, 934
MroXI GAANNNNTTC 7 cut(s) 575, 1950, 2898, 3963, 4191, 6451, 6930
MscI TGGCCA 3 cut(s) 3590, 5942, 7041
MslI CAYNNNNRTG 8 cut(s) 2781, 2826, 2922, 3374, 4509, 5639, 6726, 6752
Msp20I TGGCCA 3 cut(s) 3590, 5942, 7041
MspA1I CMGCKG 9 cut(s) 626, 824, 854, 884, 914, 944, 1541, 2263, 7037
MspCI CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
MunI CAATTG 4 cut(s) 2560, 3660, 4281, 5755
Mva1269I GAATGC 4 cut(s) 2345, 3496, 4470, 6349
MvnI CGCG 3 cut(s) 1081, 3667, 4931
NciI CCSGG 3 cut(s) 6122, 7107, 7271
NcoI CCATGG 5 cut(s) 1291, 1779, 2776, 6189, 6903
NdeI CATATG 5 cut(s) 2890, 3195, 3613, 4495, 5580
NsiI ATGCAT 2 cut(s) 1075, 5188
NspI RCATGY 4 cut(s) 1736, 1976, 2825, 3463
NspV TTCGAA 1 cut(s) 564
OliI CACNNNNGTG 2 cut(s) 4509, 6726
PaeI GCATGC 1 cut(s) 2825
PagI TCATGA 3 cut(s) 1930, 3454, 6109
PaqCI CACCTGC 1 cut(s) 1459
PasI CCCWGGG 1 cut(s) 1244
PciSI GCTCTTC 4 cut(s) 2046, 3870, 4281, 6576
PcsI WCGNNNNNNNCGW 4 cut(s) 1842, 3814, 4459, 6559
PctI GAATGC 4 cut(s) 2345, 3496, 4470, 6349
PdmI GAANNNNTTC 7 cut(s) 575, 1950, 2898, 3963, 4191, 6451, 6930
PflFI GACNNNGTC 2 cut(s) 3752, 7186
PflMI CCANNNNNTGG 2 cut(s) 2425, 5231
PpuMI RGGWCCY 4 cut(s) 1363, 2929, 5136, 6296
PshAI GACNNNNGTC 2 cut(s) 196, 3684
PshBI ATTAAT 1 cut(s) 1433
PsiI TTATAA 1 cut(s) 2865
Psp124BI GAGCTC 2 cut(s) 728, 4603
Psp1406I AACGTT 2 cut(s) 2423, 5206
Psp5II RGGWCCY 4 cut(s) 1363, 2929, 5136, 6296
PspFI CCCAGC 1 cut(s) 2148
PspOMI GGGCCC 1 cut(s) 2748
PspPPI RGGWCCY 4 cut(s) 1363, 2929, 5136, 6296
PstI CTGCAG 4 cut(s) 712, 1470, 1822, 1903
PstNI CAGNNNCTG 8 cut(s) 45, 1552, 2018, 2389, 3422, 3644, 6521, 6983
PsuI RGATCY 7 cut(s) 680, 1220, 1549, 1813, 3629, 6637, 7192
PsyI GACNNNGTC 2 cut(s) 3752, 7186
PvuII CAGCTG 8 cut(s) 626, 824, 854, 884, 914, 944, 1541, 7037
RsaI GTAC 7 cut(s) 28, 1739, 3030, 3074, 4126, 4375, 4583
RsaNI GTAC 7 cut(s) 27, 1738, 3029, 3073, 4125, 4374, 4582
RseI CAYNNNNRTG 8 cut(s) 2781, 2826, 2922, 3374, 4509, 5639, 6726, 6752
SacI GAGCTC 2 cut(s) 728, 4603
SalI GTCGAC 1 cut(s) 5961
SapI GCTCTTC 4 cut(s) 2046, 3870, 4281, 6576
SbfI CCTGCAGG 2 cut(s) 1470, 1822
SdaI CCTGCAGG 2 cut(s) 1470, 1822
SfcI CTRYAG 7 cut(s) 708, 954, 1466, 1818, 1899, 3441, 6534
SfuI TTCGAA 1 cut(s) 564
SinI GGWCC 9 cut(s) 83, 1363, 2929, 3602, 5099, 5136, 6152, 6296, 6962
SmiMI CAYNNNNRTG 8 cut(s) 2781, 2826, 2922, 3374, 4509, 5639, 6726, 6752
SphI GCATGC 1 cut(s) 2825
Sse8387I CCTGCAGG 2 cut(s) 1470, 1822
SspI AATATT 3 cut(s) 1909, 3232, 3334
SstI GAGCTC 2 cut(s) 728, 4603
TaiI ACGT 6 cut(s) 67, 2074, 2426, 4408, 4648, 5209
TaqII GACCGA 2 cut(s) 3260, 6543
TatI WGTACW 5 cut(s) 26, 1737, 3028, 3072, 4581
TauI GCSGC 2 cut(s) 4769, 6199
TspGWI ACGGA 7 cut(s) 2267, 4142, 4616, 5101, 5726, 6014, 7175
Tth111I GACNNNGTC 2 cut(s) 3752, 7186
Van91I CCANNNNNTGG 2 cut(s) 2425, 5231
Vha464I CTTAAG 6 cut(s) 320, 3506, 3968, 4861, 6724, 7118
VpaK11BI GGWCC 9 cut(s) 83, 1363, 2929, 3602, 5099, 5136, 6152, 6296, 6962
VspI ATTAAT 1 cut(s) 1433
XagI CCTNNNNNAGG 1 cut(s) 1370
XceI RCATGY 4 cut(s) 1736, 1976, 2825, 3463
XcmI CCANNNNNNNNNTGG 3 cut(s) 5569, 6099, 6187
XmiI GTMKAC 2 cut(s) 2986, 5962
XmnI GAANNNNTTC 7 cut(s) 575, 1950, 2898, 3963, 4191, 6451, 6930
Zsp2I ATGCAT 2 cut(s) 1075, 5188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.