pycom10g19330

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005784
Physical Location & Seq
Reverse (-)
22445572 .. 22450540
4969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g19330.3

Sequence Viewer

Length: 3432 bp
ATGAACCGAGCACATCGAATTGGACAGTCAAAACGACTTCTGGTATATCGGCTTGTAGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAAAAGAAACTTATGCTTGATCAGCTTTTTGTGAATAAGTCGGGATCCCAAAAAGAAGTGGAGGATATCATAAAATGGGGAACAGAGGAGCTTTTTAATGATTCTCCTAGCACTGATGGAAAAGATACCGATGAAAATAACAGTAACAAAGATGAGGCAGTGGCAGATGTAGAACATAAGCATAGGAAGCGGACTGGTGGCTTGGGAGACGTTTATACGGATAAATGCACAGATAGCAGCAACAAGATTGTATGGGATGAAAATGCAATTTCAAAGTTGCTTGACCGTTCAGACCTTCAGTCTAGTTCAACTGATATTGCTGAAGGAGAAATGGAAAATGACATGCTTGGCTCAGTAAAGGCCACTGAATGGAATGAGGAACCAGCTGAAGAACAGGGAGCTGAATCACCTCCAGGTGCAAGTGATGATACCAGTGTACAAAATACTGAAAGGAAAGAGGATAACATGGTTACTGAAGAAAATGAATGGGACAGACTTTTGCGCCTTAGGTGGGAGAAATACCAAAGTGAGGAGGAAGCAGCTCTTGGTCGTGGGAAGCGCCAGCGGAAAGCGGTTTCGTACAGAGAAGCATATGCTGCACACCCAACCGAAACATTGAGTGAGGGTGCTGACGAGGAGCATGAGCCCGAACCAGAGCCAGAGAGGGAGTACACACCTGCTGGACGAGCTCTAAAAGAAAAGTTTGCTAAGCTCCGAGCTAGACAAAAAGAACGACTTGCTCAGAGGAATGCAGTTGAAGAACCTCAACCTAGTGAGGGACTACCTGTTGAGTCACTTCCTCAGGGTCCTACCAACACTGCTAAAGATGGGGATCAAGCGACTGAATTAGTTCAGTTTCTCAGAGAGAGGCCTTCTGTAATTGACTTGGAGGACGATAAGTTAGATCCCCCTAAGGCCAAGACTGATTCTCCTTTACGATTGGGCCGGCTATCGAAGCATAAAAGCAGTCACCTTGATCTTTCTGTTAATTCTCTTGACTACATGTCACCTGACATTTTCCTTCCCAGTCACCAGGTTCCGGGAACAAGCTCACTGCTCTCAAATAATTTATTACCTGTTCTGGGACTCTGTGCTCCCAATGCTAGGCAAATAGGATCATCAAATAAGAAATTTTCAAGGTCAAACGGTAGACAAAAAGGAGCTCGGCCAGAGTTTCCATTTAGTCTGGATCCTCGGTCTGGGACTGTGAATGAGACAGAAGTAAATGGTGATGACATGAAACTATCAGATGCACCAGCTGAAGTATCACGTCTCAAGAATAAGTTGAATAACATCCCAAATGGTGGTTTTCCATTTAGGCCGTATCCTCCGCCTGGTCAAGGAAGCAGTCATGATTGTCCGGAAAGTTCCGGTGCTTCCTTTTCTGATTTCCATGAAAAGATGGCATTGCCTAACTTACCATTTGATGAGAAATTGCTACCAAGATTCCCACTTGGAGCAAAGAACATGCTGAGTCCACATCTAGACTTCTTACCTAACTTGTCATTGGGTAGCAGACTCGAATCTGCGAGTGGCTCCTTGCAAGAACTTCCAACAATGTCATTGTTTCCGAATTTAAAATTTCCGCCAGATGCACCCAGATATAATCAGCAAGATAGAGATGTGCCTCCCTCCTTGGGTTTGGGACATATGCCAACTAATTTTCCATCTTTTCCTGATAACCACCGGAAGGTGCTCGAAAACATAATGATGAGGACTGGTCCTGGATCGAGCAACTTATTCAAAAAGAAATTTAAGGCAGATGTGTGGACGGAAGATGAACTTGATTTTCTATGGATTGGTGTTCGTAGGCATGGAAGGGGCAATTGGGATGCAATGCTGAGAGACCCGAGGTTGAAGTTCTCAAAGTTTAAAACTTCAGAAGATCTGTTAGCTAGGTGGGAGGAGGAGCAACTCAAGATTTTAGATGGGTCAGCTTTTGCTGGGTCAAAGTCAATAAAAAAGACTGCCAAATCCTCACAGTTTCCAAGCATATCGGATGGGATGATGGCACGGGCACTGCATGGAAGTAGACTTGTTACACCGCCAAAATTTCAATCCCATTTGACAGATATAAAGCTGGGTTTTACTGATCTTTCATCTGGCTTTCCGCATTTTGAATCATCTGATAGACTTGGTTTGCAGAATGAGCAATATCCCCCTATTCCATCTTGGTTTCATGATAAATTCAGGACTAATTTTTCTGAAGATTCTGCTGCCGGAGCTTCTGACAGAGCAGGTACGTCTTCCAGTGTTCCCGCTGAGCAGCCATTTGTTGTCACTTCTTTTGGTACCAGTTGCTTGGGTTCATTAGGCTTGAATTCCGCTAGCAACTATGATGTACAGAAGAAGGAAGATGAACAGGGTGCTCATAGGTACGGCAAGTTGCCTTGTGTGCTTGATAGGTCACTGAATGCTTTACGTGATACGAATAATAATTTGGGAAGAGGTGAACCCTCCAGTTCAGGATTGCTGCCTAATCTCAAGAGTGGGATTTTAAAGGGAGAAGATGTTGCTGGAAGTAGTTCTTCGAAGGACACGCTTCCCCATTGGCTACGGGAAGCTGTAAGTGTTCCTGCTAAACCTCCAGTACCTGACCTGCCACCCACAGTGTCCGCAATAGCTCAATCTGTCCGGTTGTTATATGGGGAGGAGAAGCGAAGTATTCCTCCATTTGTGATTCCTGGTCCACCTCCTTCGCTACCAAAGGATCCAAGGCGAAGTCTAAAGAAGAAAAGAAAGCAGAAATCACGTCTATTTAAGCGGGTTAAACTAGACATTGCTGGAAGAGGCCGGGACTTTCATAGCAGGCACGCTGGCGACAATGCTTCAAGCTCCATTCCTATGGCCCCATCATTTCCATTGCTTTCACAAGCAATGGCTGCAACTTCAGGCCTTTCACGGATTGAATCCGGTCTAAGTGCGCCTCTGAGTATGGTAAACCCATCATCCTCAGCGCCACATCTGAATCAGCAGAAGAAAACAACCATGGGATTGTCACCTTCCCCTGAAGTGCTTCAGTTGGTAGCATCCTGCGTTGCGCCAGGTCCACATTTGTCAGCAGCTTCTGGCATGGCAAGCACAAGCTTCCGTGACGCAAAGCCCTCATTGCCAAACTCCGTTGACCAAGTAGAACTCTTGGACTCGCAAACCGCAACTGCTAGGGTAAGAACGATGGCTAAGCAGGGATCGCCTGTTAGAACATTTGATACTGTCAGCGGGGATTCCAGCAAGACACAGTCGGATCCCCCTAGGACAGAACGACCCGATGTAGAGGAAATTTCTTCTGAGGGCACTGTCTCGATCGATCATCCCGTGAGTGATCAGGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1144

Amino Acids

125.73

Weight (kDa)

6.27

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 781
Acc36I ACCTGC 3 cut(s) 781, 2324, 2703
Acc65I GGTACC 1 cut(s) 2387
AccB1I GGYRCC 1 cut(s) 2387
AccB7I CCANNNNNTGG 2 cut(s) 465, 1400
AccI GTMKAC 2 cut(s) 1246, 2128
AccIII TCCGGA 1 cut(s) 1456
AcoI YGGCCR 1 cut(s) 1262
AcsI RAATTY 8 cut(s) 1226, 1669, 1676, 1847, 2147, 2282, 2416, 3375
AfaI GTAC 8 cut(s) 534, 677, 767, 2338, 2389, 2439, 2474, 2688
AflIII ACRYGT 1 cut(s) 1098
AhdI GACNNNNNGTC 2 cut(s) 394, 1099
AjiI CACGTC 2 cut(s) 1367, 2849
AjnI CCWGG 6 cut(s) 508, 1128, 1429, 1819, 2779, 3139
AjuI GAANNNNNNNTTGG 6 cut(s) 624, 656, 1906, 1938, 2519, 2551
Alw21I GWGCWC 6 cut(s) 13, 787, 1192, 1261, 1794, 2467
Alw26I GTCTC 5 cut(s) 297, 1304, 1373, 1935, 3400
AlwNI CAGNNNCTG 2 cut(s) 2690, 3164
Ama87I CYCGRG 1 cut(s) 1945
Aor13HI TCCGGA 1 cut(s) 1456
AoxI GGCC 9 cut(s) 456, 965, 1011, 1039, 1262, 1415, 2887, 2943, 2989
ApeKI GCWGC 9 cut(s) 88, 333, 635, 692, 2312, 2362, 2569, 2978, 3158
ApoI RAATTY 8 cut(s) 1226, 1669, 1676, 1847, 2147, 2282, 2416, 3375
Asp700I GAANNNNTTC 3 cut(s) 945, 2620, 3111
Asp718I GGTACC 1 cut(s) 2387
AspA2I CCTAGG 1 cut(s) 3347
AspLEI GCGC 6 cut(s) 81, 600, 657, 3022, 3055, 3139
AspS9I GGNCC 6 cut(s) 902, 1039, 1817, 2783, 2944, 3143
AsuC2I CCSGG 2 cut(s) 1137, 2891
AsuHPI GGTGA 7 cut(s) 495, 1058, 1095, 1118, 1337, 2558, 3087
AsuII TTCGAA 1 cut(s) 2627
AsuNHI GCTAGC 1 cut(s) 2423
AvaI CYCGRG 1 cut(s) 1945
AvaII GGWCC 4 cut(s) 902, 1817, 2783, 3143
AvrII CCTAGG 1 cut(s) 3347
AxyI CCTNAGG 3 cut(s) 602, 897, 1008
BaeGI GKGCMC 2 cut(s) 2116, 3392
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BamHI GGATCC 4 cut(s) 140, 1285, 2806, 3340
BanI GGYRCC 1 cut(s) 2387
BanII GRGCYC 3 cut(s) 744, 787, 1261
BbsI GAAGAC 1 cut(s) 2334
Bbv12I GWGCWC 6 cut(s) 13, 787, 1192, 1261, 1794, 2467
BbvCI CCTCAGC 1 cut(s) 3049
BbvI GCAGC 9 cut(s) 100, 345, 647, 679, 2299, 2374, 2556, 2965, 3170
BceAI ACGGC 2 cut(s) 1402, 2491
BciT130I CCWGG 6 cut(s) 510, 1130, 1431, 1821, 2781, 3141
BciVI GTATCC 1 cut(s) 1431
BclI TGATCA 2 cut(s) 115, 3418
BcnI CCSGG 2 cut(s) 1137, 2891
BcoDI GTCTC 5 cut(s) 297, 1304, 1373, 1935, 3400
BfoI RGCGCY 2 cut(s) 658, 3056
BfuAI ACCTGC 3 cut(s) 781, 2324, 2703
BfuI GTATCC 1 cut(s) 1431
BglII AGATCT 1 cut(s) 1981
BisI GCNGC 9 cut(s) 89, 334, 636, 693, 2313, 2363, 2570, 2979, 3159
BlnI CCTAGG 1 cut(s) 3347
BlpI GCTNAGC 3 cut(s) 804, 2358, 3276
BlsI GCNGC 9 cut(s) 90, 335, 637, 694, 2314, 2364, 2571, 2980, 3160
Bme1390I CCNGG 8 cut(s) 510, 1130, 1137, 1431, 1821, 2781, 2891, 3141
Bme18I GGWCC 4 cut(s) 902, 1817, 2783, 3143
BmeRI GACNNNNNGTC 2 cut(s) 394, 1099
BmeT110I CYCGRG 1 cut(s) 1945
BmgBI CACGTC 2 cut(s) 1367, 2849
BmgT120I GGNCC 6 cut(s) 902, 1039, 1817, 2783, 2944, 3143
BmrFI CCNGG 8 cut(s) 510, 1130, 1137, 1431, 1821, 2781, 2891, 3141
BmrI ACTGGG 1 cut(s) 1116
BmsI GCATC 5 cut(s) 90, 1336, 1678, 1918, 3134
BmtI GCTAGC 1 cut(s) 2427
BmuI ACTGGG 1 cut(s) 1116
BpiI GAAGAC 1 cut(s) 2334
BpmI CTGGAG 3 cut(s) 492, 2539, 2667
Bpu10I CCTNAGC 1 cut(s) 3049
Bpu1102I GCTNAGC 3 cut(s) 804, 2358, 3276
Bpu14I TTCGAA 1 cut(s) 2627
BpuEI CTTGAG 3 cut(s) 1355, 1997, 2564
BpuMI CCSGG 2 cut(s) 1137, 2891
Bsa29I ATCGAT 1 cut(s) 3402
BsaAI YACGTR 1 cut(s) 2519
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 1935
BsaJI CCNNGG 6 cut(s) 1289, 1731, 1946, 2810, 3084, 3347
BsaWI WCCGGW 5 cut(s) 1456, 1466, 1782, 2730, 3008
BsaXI ACNNNNNCTCC 4 cut(s) 1009, 1039, 2592, 2622
Bse118I RCCGGY 1 cut(s) 1041
Bse1I ACTGG 8 cut(s) 295, 528, 1122, 1819, 2346, 2391, 2556, 2684
Bse21I CCTNAGG 3 cut(s) 602, 897, 1008
Bse3DI GCAATG 6 cut(s) 1502, 1938, 2874, 2957, 2979, 3203
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 1 cut(s) 1456
BseBI CCWGG 6 cut(s) 510, 1130, 1431, 1821, 2781, 3141
BseCI ATCGAT 1 cut(s) 3402
BseDI CCNNGG 6 cut(s) 1289, 1731, 1946, 2810, 3084, 3347
BseGI GGATG 8 cut(s) 358, 1389, 1933, 2101, 2106, 3044, 3125, 3406
BseJI GATNNNNATC 1 cut(s) 927
BseMI GCAATG 6 cut(s) 1502, 1938, 2874, 2957, 2979, 3203
BseNI ACTGG 8 cut(s) 295, 528, 1122, 1819, 2346, 2391, 2556, 2684
BseRI GAGGAG 6 cut(s) 197, 641, 746, 2015, 2018, 2762
BseSI GKGCMC 2 cut(s) 2116, 3392
BseXI GCAGC 9 cut(s) 100, 345, 647, 679, 2299, 2374, 2556, 2965, 3170
BseYI CCCAGC 2 cut(s) 2039, 2176
BsgI GTGCAG 1 cut(s) 678
Bsh1285I CGRYCG 1 cut(s) 3402
BshFI GGCC 9 cut(s) 458, 967, 1013, 1041, 1264, 1417, 2889, 2945, 2991
BshNI GGYRCC 1 cut(s) 2387
BshVI ATCGAT 1 cut(s) 3402
BsiEI CGRYCG 1 cut(s) 3402
BsiHKAI GWGCWC 6 cut(s) 13, 787, 1192, 1261, 1794, 2467
BsiHKCI CYCGRG 1 cut(s) 1945
BsiSI CCGG 9 cut(s) 1042, 1136, 1457, 1467, 1783, 2316, 2731, 2890, 3009
BslFI GGGAC 6 cut(s) 599, 888, 1194, 1312, 1755, 2906
BsmAI GTCTC 5 cut(s) 297, 1304, 1373, 1935, 3400
BsmBI CGTCTC 2 cut(s) 297, 1373
BsmFI GGGAC 6 cut(s) 599, 888, 1194, 1312, 1755, 2906
BsmI GAATGC 2 cut(s) 850, 2515
BsnI GGCC 9 cut(s) 458, 967, 1013, 1041, 1264, 1417, 2889, 2945, 2991
Bso31I GGTCTC 1 cut(s) 1935
BsoBI CYCGRG 1 cut(s) 1945
Bsp119I TTCGAA 1 cut(s) 2627
Bsp1286I GDGCHC 9 cut(s) 13, 744, 787, 1192, 1261, 1794, 2116, 2467, 3392
Bsp13I TCCGGA 1 cut(s) 1456
Bsp1407I TGTACA 2 cut(s) 532, 2437
Bsp1720I GCTNAGC 3 cut(s) 804, 2358, 3276
Bsp19I CCATGG 1 cut(s) 3084
BspANI GGCC 9 cut(s) 458, 967, 1013, 1041, 1264, 1417, 2889, 2945, 2991
BspDI ATCGAT 1 cut(s) 3402
BspEI TCCGGA 1 cut(s) 1456
BspHI TCATGA 2 cut(s) 1447, 2275
BspMI ACCTGC 3 cut(s) 781, 2324, 2703
BspOI GCTAGC 1 cut(s) 2427
BspQI GCTCTTC 1 cut(s) 69
BspT104I TTCGAA 1 cut(s) 2627
BspT107I GGYRCC 1 cut(s) 2387
BspTNI GGTCTC 1 cut(s) 1935
BsrDI GCAATG 6 cut(s) 1502, 1938, 2874, 2957, 2979, 3203
BsrFI RCCGGY 1 cut(s) 1041
BsrGI TGTACA 2 cut(s) 532, 2437
BsrI ACTGG 8 cut(s) 295, 528, 1122, 1819, 2346, 2391, 2556, 2684
BssAI RCCGGY 1 cut(s) 1041
BssECI CCNNGG 6 cut(s) 1289, 1731, 1946, 2810, 3084, 3347
BssT1I CCWWGG 4 cut(s) 1731, 2810, 3084, 3347
Bst2UI CCWGG 6 cut(s) 510, 1130, 1431, 1821, 2781, 3141
Bst6I CTCTTC 3 cut(s) 69, 2536, 2878
BstAPI GCANNNNNTGC 2 cut(s) 692, 2978
BstAUI TGTACA 2 cut(s) 532, 2437
BstBAI YACGTR 1 cut(s) 2519
BstBI TTCGAA 1 cut(s) 2627
BstC8I GCNNGC 8 cut(s) 93, 659, 1043, 2425, 2906, 2910, 2914, 3175
BstDSI CCRYGG 1 cut(s) 3084
BstENI CCTNNNNNAGG 1 cut(s) 1434
BstF5I GGATG 8 cut(s) 358, 1389, 1933, 2101, 2106, 3044, 3125, 3406
BstH2I RGCGCY 2 cut(s) 658, 3056
BstHHI GCGC 6 cut(s) 81, 600, 657, 3022, 3055, 3139
BstMAI GTCTC 5 cut(s) 297, 1304, 1373, 1935, 3400
BstMCI CGRYCG 1 cut(s) 3402
BstNI CCWGG 6 cut(s) 510, 1130, 1431, 1821, 2781, 3141
BstNSI RCATGY 3 cut(s) 442, 1102, 1567
BstSCI CCNGG 8 cut(s) 508, 1128, 1135, 1429, 1819, 2779, 2889, 3139
BstSLI GKGCMC 2 cut(s) 2116, 3392
BstV1I GCAGC 9 cut(s) 100, 345, 647, 679, 2299, 2374, 2556, 2965, 3170
BstV2I GAAGAC 1 cut(s) 2334
BstX2I RGATCY 6 cut(s) 140, 1000, 1285, 1981, 2806, 3340
BstXI CCANNNNNNTGG 2 cut(s) 2398, 2941
BstYI RGATCY 6 cut(s) 140, 1000, 1285, 1981, 2806, 3340
Bsu15I ATCGAT 1 cut(s) 3402
Bsu36I CCTNAGG 3 cut(s) 602, 897, 1008
BsuI GTATCC 1 cut(s) 1431
BsuRI GGCC 9 cut(s) 458, 967, 1013, 1041, 1264, 1417, 2889, 2945, 2991
BsuTUI ATCGAT 1 cut(s) 3402
BtgI CCRYGG 1 cut(s) 3084
BtrI CACGTC 2 cut(s) 1367, 2849
BtsCI GGATG 8 cut(s) 358, 1389, 1933, 2101, 2106, 3044, 3125, 3406
BtsI GCAGTG 4 cut(s) 261, 912, 1148, 2114
BveI ACCTGC 3 cut(s) 781, 2324, 2703
Cac8I GCNNGC 8 cut(s) 93, 659, 1043, 2425, 2906, 2910, 2914, 3175
CaiI CAGNNNCTG 2 cut(s) 2690, 3164
CciI TCATGA 2 cut(s) 1447, 2275
CfoI GCGC 6 cut(s) 81, 600, 657, 3022, 3055, 3139
Cfr10I RCCGGY 1 cut(s) 1041
Cfr13I GGNCC 6 cut(s) 902, 1039, 1817, 2783, 2944, 3143
ClaI ATCGAT 1 cut(s) 3402
CseI GACGC 1 cut(s) 3200
CsiI ACCWGGT 1 cut(s) 1128
Csp6I GTAC 8 cut(s) 533, 676, 766, 2337, 2388, 2438, 2473, 2687
CviQI GTAC 8 cut(s) 533, 676, 766, 2337, 2388, 2438, 2473, 2687
DraI TTTAAA 3 cut(s) 1674, 1969, 2595
DriI GACNNNNNGTC 2 cut(s) 394, 1099
EaeI YGGCCR 1 cut(s) 1262
Eam1104I CTCTTC 3 cut(s) 69, 2536, 2878
Eam1105I GACNNNNNGTC 2 cut(s) 394, 1099
EarI CTCTTC 3 cut(s) 69, 2536, 2878
EciI GGCGGA 2 cut(s) 1416, 1671
Ecl136II GAGCTC 2 cut(s) 785, 1259
Eco130I CCWWGG 4 cut(s) 1731, 2810, 3084, 3347
Eco147I AGGCCT 2 cut(s) 967, 2991
Eco24I GRGCYC 3 cut(s) 744, 787, 1261
Eco31I GGTCTC 1 cut(s) 1935
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 4 cut(s) 902, 1817, 2783, 3143
Eco53kI GAGCTC 2 cut(s) 785, 1259
Eco81I CCTNAGG 3 cut(s) 602, 897, 1008
Eco88I CYCGRG 1 cut(s) 1945
EcoICRI GAGCTC 2 cut(s) 785, 1259
EcoNI CCTNNNNNAGG 1 cut(s) 1434
EcoO109I RGGNCCY 1 cut(s) 902
EcoRI GAATTC 1 cut(s) 2416
EcoRII CCWGG 6 cut(s) 508, 1128, 1429, 1819, 2779, 3139
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 4 cut(s) 1731, 2810, 3084, 3347
EcoT38I GRGCYC 3 cut(s) 744, 787, 1261
ErhI CCWWGG 4 cut(s) 1731, 2810, 3084, 3347
Esp3I CGTCTC 2 cut(s) 297, 1373
FalI AAGNNNNNCTT 8 cut(s) 624, 656, 816, 848, 1863, 1895, 2608, 2640
FaqI GGGAC 6 cut(s) 599, 888, 1194, 1312, 1755, 2906
FauI CCCGC 3 cut(s) 2362, 2853, 3308
FauNDI CATATG 2 cut(s) 688, 1746
FbaI TGATCA 2 cut(s) 115, 3418
FblI GTMKAC 2 cut(s) 1246, 2128
Fnu4HI GCNGC 9 cut(s) 89, 334, 636, 693, 2313, 2363, 2570, 2979, 3159
FokI GGATG 8 cut(s) 365, 1376, 1940, 2108, 2113, 3031, 3112, 3393
FriOI GRGCYC 3 cut(s) 744, 787, 1261
Fsp4HI GCNGC 9 cut(s) 89, 334, 636, 693, 2313, 2363, 2570, 2979, 3159
GlaI GCGC 6 cut(s) 80, 599, 656, 3021, 3054, 3138
GluI GCNGC 9 cut(s) 89, 334, 636, 693, 2313, 2363, 2570, 2979, 3159
GsaI CCCAGC 2 cut(s) 2043, 2180
GsuI CTGGAG 3 cut(s) 492, 2539, 2667
HaeII RGCGCY 2 cut(s) 658, 3056
HaeIII GGCC 9 cut(s) 458, 967, 1013, 1041, 1264, 1417, 2889, 2945, 2991
HapII CCGG 9 cut(s) 1042, 1136, 1457, 1467, 1783, 2316, 2731, 2890, 3009
HgaI GACGC 1 cut(s) 3200
HhaI GCGC 6 cut(s) 81, 600, 657, 3022, 3055, 3139
Hin6I GCGC 6 cut(s) 79, 598, 655, 3020, 3053, 3137
HinP1I GCGC 6 cut(s) 79, 598, 655, 3020, 3053, 3137
HincII GTYRAC 1 cut(s) 3220
HindII GTYRAC 1 cut(s) 3220
HindIII AAGCTT 1 cut(s) 3181
HpaII CCGG 9 cut(s) 1042, 1136, 1457, 1467, 1783, 2316, 2731, 2890, 3009
HphI GGTGA 7 cut(s) 495, 1058, 1095, 1118, 1337, 2558, 3087
HpyCH4IV ACGT 5 cut(s) 306, 1366, 2339, 2518, 2848
HpySE526I ACGT 5 cut(s) 306, 1366, 2339, 2518, 2848
HspAI GCGC 6 cut(s) 79, 598, 655, 3020, 3053, 3137
Kpn2I TCCGGA 1 cut(s) 1456
KpnI GGTACC 1 cut(s) 2391
KroI GCCGGC 1 cut(s) 1041
KroNI GCCGGC 1 cut(s) 1043
Ksp22I TGATCA 2 cut(s) 115, 3418
LguI GCTCTTC 1 cut(s) 69
Lsp1109I GCAGC 9 cut(s) 100, 345, 647, 679, 2299, 2374, 2556, 2965, 3170
LweI GCATC 5 cut(s) 90, 1336, 1678, 1918, 3134
MabI ACCWGGT 1 cut(s) 1128
MaeII ACGT 5 cut(s) 306, 1366, 2339, 2518, 2848
MfeI CAATTG 1 cut(s) 1921
MflI RGATCY 6 cut(s) 140, 1000, 1285, 1981, 2806, 3340
MhlI GDGCHC 9 cut(s) 13, 744, 787, 1192, 1261, 1794, 2116, 2467, 3392
MlyI GAGTC 5 cut(s) 896, 1176, 1579, 1608, 3233
MmeI TCCRAC 2 cut(s) 1673, 3318
MroI TCCGGA 1 cut(s) 1456
MroNI GCCGGC 1 cut(s) 1041
MroXI GAANNNNTTC 3 cut(s) 945, 2620, 3111
MseI TTAA 8 cut(s) 192, 1083, 1673, 1851, 1968, 2594, 2856, 2865
MslI CAYNNNNRTG 2 cut(s) 1805, 2939
MspA1I CMGCKG 5 cut(s) 482, 661, 1355, 2357, 3315
MspI CCGG 9 cut(s) 1042, 1136, 1457, 1467, 1783, 2316, 2731, 2890, 3009
MspR9I CCNGG 8 cut(s) 510, 1130, 1137, 1431, 1821, 2781, 2891, 3141
MunI CAATTG 1 cut(s) 1921
Mva1269I GAATGC 2 cut(s) 850, 2515
MvaI CCWGG 6 cut(s) 510, 1130, 1431, 1821, 2781, 3141
NaeI GCCGGC 1 cut(s) 1043
NciI CCSGG 2 cut(s) 1137, 2891
NcoI CCATGG 1 cut(s) 3084
NdeI CATATG 2 cut(s) 688, 1746
NgoMIV GCCGGC 1 cut(s) 1041
NheI GCTAGC 1 cut(s) 2423
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 8 cut(s) 888, 1064, 1101, 1124, 2374, 2502, 3093, 3188
NspI RCATGY 3 cut(s) 442, 1102, 1567
NspV TTCGAA 1 cut(s) 2627
PagI TCATGA 2 cut(s) 1447, 2275
PaqCI CACCTGC 1 cut(s) 781
PceI AGGCCT 2 cut(s) 967, 2991
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 3408
PctI GAATGC 2 cut(s) 850, 2515
PdiI GCCGGC 1 cut(s) 1043
PdmI GAANNNNTTC 3 cut(s) 945, 2620, 3111
PflFI GACNNNGTC 1 cut(s) 3334
PflMI CCANNNNNTGG 2 cut(s) 465, 1400
PfoI TCCNGGA 2 cut(s) 1135, 1819
PkrI GCNGC 9 cut(s) 90, 335, 637, 694, 2314, 2364, 2571, 2980, 3160
Ple19I CGATCG 1 cut(s) 3402
PleI GAGTC 5 cut(s) 895, 1176, 1578, 1608, 3233
PpsI GAGTC 5 cut(s) 895, 1176, 1578, 1608, 3233
Ppu21I YACGTR 1 cut(s) 2519
PpuMI RGGWCCY 1 cut(s) 902
PscI ACATGT 1 cut(s) 1098
Psp124BI GAGCTC 2 cut(s) 787, 1261
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 6 cut(s) 508, 1128, 1429, 1819, 2779, 3139
PspFI CCCAGC 2 cut(s) 2039, 2176
PspGI CCWGG 6 cut(s) 508, 1128, 1429, 1819, 2779, 3139
PspPI GGNCC 6 cut(s) 902, 1039, 1817, 2783, 2944, 3143
PspPPI RGGWCCY 1 cut(s) 902
PsrI GAACNNNNNNTAC 2 cut(s) 3289, 3321
PstNI CAGNNNCTG 2 cut(s) 2690, 3164
PsuI RGATCY 6 cut(s) 140, 1000, 1285, 1981, 2806, 3340
PsyI GACNNNGTC 1 cut(s) 3334
PvuI CGATCG 1 cut(s) 3402
PvuII CAGCTG 2 cut(s) 482, 1355
RsaI GTAC 8 cut(s) 534, 677, 767, 2338, 2389, 2439, 2474, 2688
RsaNI GTAC 8 cut(s) 533, 676, 766, 2337, 2388, 2438, 2473, 2687
RseI CAYNNNNRTG 2 cut(s) 1805, 2939
SacI GAGCTC 2 cut(s) 787, 1261
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 8 cut(s) 192, 1083, 1673, 1851, 1968, 2594, 2856, 2865
SatI GCNGC 9 cut(s) 89, 334, 636, 693, 2313, 2363, 2570, 2979, 3159
Sau96I GGNCC 6 cut(s) 902, 1039, 1817, 2783, 2944, 3143
SchI GAGTC 5 cut(s) 896, 1176, 1579, 1608, 3233
ScrFI CCNGG 8 cut(s) 510, 1130, 1137, 1431, 1821, 2781, 2891, 3141
SduI GDGCHC 9 cut(s) 13, 744, 787, 1192, 1261, 1794, 2116, 2467, 3392
SexAI ACCWGGT 1 cut(s) 1128
SfaNI GCATC 5 cut(s) 90, 1336, 1678, 1918, 3134
SfuI TTCGAA 1 cut(s) 2627
SinI GGWCC 4 cut(s) 902, 1817, 2783, 3143
SmiMI CAYNNNNRTG 2 cut(s) 1805, 2939
SmlI CTYRAG 3 cut(s) 1370, 2012, 2579
SmoI CTYRAG 3 cut(s) 1370, 2012, 2579
SseBI AGGCCT 2 cut(s) 967, 2991
SstI GAGCTC 2 cut(s) 787, 1261
StuI AGGCCT 2 cut(s) 967, 2991
StyD4I CCNGG 8 cut(s) 508, 1128, 1135, 1429, 1819, 2779, 2889, 3139
StyI CCWWGG 4 cut(s) 1731, 2810, 3084, 3347
TaiI ACGT 5 cut(s) 309, 1369, 2342, 2521, 2851
TaqI TCGA 8 cut(s) 16, 1049, 1617, 1794, 1826, 2627, 3398, 3402
TaqII GACCGA 1 cut(s) 1281
TatI WGTACW 3 cut(s) 532, 765, 2437
Tru1I TTAA 8 cut(s) 192, 1083, 1673, 1851, 1968, 2594, 2856, 2865
Tru9I TTAA 8 cut(s) 192, 1083, 1673, 1851, 1968, 2594, 2856, 2865
TseFI GTSAC 8 cut(s) 888, 1064, 1101, 1124, 2374, 2502, 3093, 3188
TseI GCWGC 9 cut(s) 88, 333, 635, 692, 2312, 2362, 2569, 2978, 3158
Tsp45I GTSAC 8 cut(s) 888, 1064, 1101, 1124, 2374, 2502, 3093, 3188
TspGWI ACGGA 5 cut(s) 329, 1883, 3013, 3176, 3205
Tth111I GACNNNGTC 1 cut(s) 3334
Van91I CCANNNNNTGG 2 cut(s) 465, 1400
VpaK11BI GGWCC 4 cut(s) 902, 1817, 2783, 3143
XagI CCTNNNNNAGG 1 cut(s) 1434
XapI RAATTY 8 cut(s) 1226, 1669, 1676, 1847, 2147, 2282, 2416, 3375
XbaI TCTAGA 1 cut(s) 1579
XceI RCATGY 3 cut(s) 442, 1102, 1567
XmaJI CCTAGG 1 cut(s) 3347
XmiI GTMKAC 2 cut(s) 1246, 2128
XmnI GAANNNNTTC 3 cut(s) 945, 2620, 3111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.