Rh5DG150200

DUF1087

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005784
Physical Location & Seq
Forward (+)
15226072 .. 15239375
13304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG150200.1

Sequence Viewer

Length: 5286 bp
ATGAAGGATAACAGCTCGTCAACAAGTACAATGATTAACAGAAACTGGGTCTTGAAACGAAAACGTAGAAAGCTTCCTTATGGACCTGCTCTATCTAATGGCAAAGAAGACAGTTCAGCAGCATCAGAATCTCAAGGGAAGACATCTTCTTCCGCTAAACGACGGCTGACTAATGAAATTATTTCTGATCGACTTTCGTCCAAGAAGAAAGGAAACGATGGGTATTACTATGAATGTGTGGTCTGTGACCTCGGTGGTAACTTGTTGTGTTGTGATAGTTGTCCCCAAACCTATCATCTCCAGTGTCTTAATCCACCTCTTAAGCGGATTCCGAATGGGAAGTGGCAGTGCCCAAACTGCTGTCAGAAAAGTGACGTGCCAGAGCCTTTAAATTATCTAGCTGATAACATTTCAAAACGAGCAAGGACAAAGATTGCCACCTCTAAGTCCAAAGCTGAAGTTAAGTCATCTGACAGAGAGAAAGTTTCGCAGATTTTTGGGAACACCATTGTTGCAAAGAAAAGATCATCAAGCAAAGGGAAAGCTCATCGAGTTAAATCCTTCGAAAAGAAACCATTCTCCCAGATAGATATATCTTCTAGCACGAAGCCCAGTCATTCATCAGCTGGCTGTTCTGTGGAGGGTATTTCCTTGTCTGAGAATGTTGGCGATGAAAATAGATCTAATCTTTCTCCACCAGATGATTCCGCAGACAAAAAGTTGAGCTCTCCAGCTAAAGAGGTTTCATCTCATTCTAAAATATCAGAGACAAATGAAGAAGCTCCGGAGGCAGCTGTGACAACTGGAGAAGCTCCAGAGGCAGCTGTGACAACTGGAGAAGCTCTGGAGGCAGCTGTGACAACTGGAGAAGCTCCGGAGGCAGCTGTGACAACTGGAGAAGCTCCTGAGGCATCTGTGCCAAATGGAGAAGCTCCTGATGCATCTGTGACAAATGGAGAAGCTCCTGAGGCATCTGTCCTTCCTGAAGTGAAGCCTGACCCGTCTTGTGATGATGCATCTCCGCGTAAGACCATTGTTCTTGCCATCAGTGCTGCTGCTGGCAAGGCTAGAAAAAGAAAACACAAAGGCAATAATGAGAAGAGTAAAAAGAAGCGCAGGACAGAGAAGAGGAAAACTGTCATTGGTGTTTCTAAACAGTCTGGATCTAAAGCAAATACCTCAAGCCCAGGGACTCGTATTCGTAAAACACTTCGTAAGCATAAATCTCTCAACCATGGGGTTTCTGCATCTTTGGCAAGGGAGGATGTCACAACCAAGGGTTCAGATGTCCAGATGAAAGACGAGGACCTTACTGAGGAAGCAAAAGACCAATTTCATAATGCAGATAAAGCCGGAAATCATGGAGTCGAAACATTAATGCAGAAGGATAGTCTTACTGCTGAACTCCTGCAGGTGGATCGGGTTTTGGGATGTCGAATTCATGGTAATCATGCTGCTGCTTCTTGTCACTTATCAGTGACAGCTGCCCAGGACCTGAGTTCTGATGATGCACAAGTCTCAGAAAATAATAACAGACTATTACAGGAAAATTTTGCTTGCGAAACTGTTATGGATGTTGGGGCGGCTAAAAATCTCACTGAGGGTTGTCAAGGAGTTGTTAAGGGTGTTGATGGGGATGATGGGGATGACAGCAAGAAGGAGGATATAAGAGTGGACAAACTGCATGTGTACAGAAGGTCTATGAACAAAGAAGGTAAAAGAGCTAATTCCATGGATTTGTCTAGGAAAGATACCAAGGAGTTGGATCCTGCAGGCATAATTGATCACGATCCAAACGAATCTGCTTTAAATGCTGATGATCCAGAAAAAACAAATGAAGAAGTAGTGACTCCGGAGAATATTGATGTCAATTTGAACAGTCATGATAAAGATAAAGAAGAGGTTCTGGAAATTTGTGAATCGCATGTTTCTGCTGATACCAATGACAAGAGAGATGTCAATACAGAAGCTGGAACTGATATCTGTGCTGAAAACAAAAGTGAAGATCCCACACTGGCTGAACGTGCAGGTGATGGTGTTGAGACGTTATCTTATGAATTTTTAGTTAAGTGGGTAGGTAAGTCTCATATTCATAATAGCTGGGTTTCTGAATCTGAGTTAAAAGTCCTGGCAAAGAGGAAACTAGAAAACTATAAGGCAAAGTATGGAATGGCTGTTATAAATATATGCGAGGAGAGATGGAAGCAACCACAGCGGGTGATCGCTCTCCGTGGATTTAAAGATGGTTCAGGTGAAGCTTTTGTGAAATGGACTGGTTTGCCTTATGATGAATGCACTTGGGAAAGATTGGATGAACCTGTTATGAGAAATTCTCAGAATCTGGTTGAATTATTTAGTCAGTTTGAGCACCAAACGTTGGTAAATGATGCTCTTAAAGATGATTCATCAAGAGGGAAGGTTAGCCGACAGCAAAATGAAATACTTGCTCTGACAGAGCAGCCCAAGGAACTAAAGGGGGGTTCATTGTTTCCCCATCAGCTTGAAGCACTCAATTGGTTGCGGAAGTGCTGGCATAAATCAAGAAATGTGATACTAGCTGATGAGATGGGGCTTGGTAAAACCATATCAGCTTGTGCTTTTATTTCATCATTATATTGCGAGTTCAAAGCCACTCTACCTTGTTTAGTCTTGGTTCCACTTTCCACAATGCCTAATTGGCTTGCTGAGTTTTCATTATGGGCCCCTGAATTGAATGTCGTGGAATACCATGGGTGTGCCAAAGCAAGAGCCATGATTCGCCAGTATGAATGGCATGCTAGTGTTCCAAATGAGTTGAACAAGAAGACATCCGCTTATAAATTTAATGTTCTTTTAACTACATATGAAATGGTTCTTGCTGATTCCACACATCTTCGTGGGGTCCCTTGGGAAGTTCTCATAGTTGATGAGGGCCATCGATTGAAAAATTCAGGAAGTAGACTTTTCAGCTTGCTTAACTCGTTCTCTTTCCAACATCGTGTACTGTTGACTGGCACCCCTCTTCAGAATAATCTTGGTGAGATGTACAACTTGCTTAATTTCTTGCAGCCTGCATCATTCCCTTCCCTGTCTTCATTTGAGGAGAGGTTTAATGATCTTACAACTTCAGAAAAAGTGGAGGAATTGAAAAAACTCGTTGCTCCACATATGCTTCGTAGGCTTAAAAAGGATGCTATGCAGAATATTCCCCCAAAGACCGAACGAATGGTTCCTGTTGAGTTGTCCTCCATCCAAGCTGAATACTATCGTGCAATGTTAACAAAGAATTATCAGATATTGCGGAATATTGGGAAAGGGGTTGCACAACAATCGATGCTGAACATTGTAATGCAGTTAAGGAAAGTCTGCAATCATCCATATCTGATACCAGGCACTGAGCCTGACTGTGGGTCTGTAGAGTTCCTTCATGACATGCGGATAAAAGCTTCAGCCAAGTTGACACTGTTGCATTCTATGCTTAAGATTTTGCACAAAGAGGGTCACAGAGTCCTTATCTTTTCCCAGATGACCAAGCTCCTTGATATCCTTGAGGATTATTTGGCCATCGAATTTGGACCTAAAACATATGAGAGAGTAGATGGATCTGTTGCAGTGGCTGATCGTCAATCTGCAATTGCGCGTTTTAACCAAGACAGAAGTCGATTTGTCTTTTTGTTATCAACACGCTCTTGTGGCCTTGGGATCAATTTGGCAACTGCTGACACCGTCATTATTTATGATTCTGATTTCAACCCCCATGCTGATATTCAAGCTATGAATCGAGCACATCGAATCGGACAATCAAATAGACTTCTGGTTTATAGGCTTGTAGTTCGTGCTAGTGTTGAAGAGCGCATCTTGCAGCTTGCAAAGAAGAAGCTTATGCTGGATCAGCTATTTGTGAATAAGTCAGAATCCCAAAAAGAAGTGGAAGATATTCTTAAGTGGGGAACAGAGGAACTTTTCAATGATTCTCCTGGCATGGATGGAAAAGATACAGGTGAAAATAATAGTAACAAAGATGAGGCAGTGCCAGATGTAGAGCATAAGCATAAGAAGAGGATTGGTAGCCTGGGAGATGTTTATGAAGATAAATGTACGGAAAACAGCAACAAGATTGTTTGGGATGAAACTGCGATTTTAAAGTTGCTTGACCGTGAAAATCTTCAATCTGGACTGACTGATAATGCGGAAGGAGATATGGAAAATGATATGCTTGGCTCTGTAAAGTCTATCGAATGGAATGAGGAGCCAATTGAAGAGCAGGGAGTTGAATCACCTCCTGGTGCAAGTGATGATATATGTGCTCAAAATACTGAAAGGAAAGAGGATAATGTGGTTAATGGTACAGAAGAAAATGAATGGGACAGACTCTTACGTCTTAGATGGGAGAAATATCAAAGTGAGGAGGAAGCAGCTCTTGGTCGTGGTAAGCGAATGCGGAAAGCTGTTTCTTACAGGGAAGCATATGCTGCACACCCAAGTGAAACATTAACTGAGAGTGGTGGTGTTGAAGATGAGCGTGAGCCCGAACCAGAGCCAGAGCGGGAGTACACACCCGCAGGACGAGCTCTAAAAGCAAAGTATGCTAAGCTCCGTGCTAGGCAAAAAGAACGTCTAGCTCAGAGGAATGAAATTGAAGAACCTCGTCCTAGTGAGGGACTGCCTGTTGAGTCACATCCTCAGTGTCCCATGAACACTGCCGAAGATGTTGATCAAGCGACTGGGGATCAAGCGGCTGGATTAGTTCAATTTCTCAGTGAGAAGTCTTCAGTAATTGACTTGGAGGATAACAAATTAGATGCTTCTAAAGCCAAGACTGACTCTCCCTTAAGATTGAGCAAGCTATCAAAGCATAAAAGCAGTCGCTTCGATCTCTCTGTTAATCCGCTTGACTACGCGTCTCCTGACATTTTATTTCCTAGACACCAACATCAGGGAACAAGCATGACTCATTCGATGCCCCCAAATAATTTGTTACCTGTTCTGGGACTGTGTGCCCCAAATGCTAGTCAAATAGAATCATCAAAAAAGAACTCAAGGTCGAATGGCAGACGGAGAGGAGCTGGACCAGAATTTCCATTTAGTCTAGCTCCTCATTCTGGGACCTTGCCTGAAACAGAAGCAAATGGTGATGAAGCAAAGCTATCAGATGCATCCGCGGAGGCATCACAGCGTTTGAAGAGTAGCATCCCAAATGGTGGCTTGCCATTTAGACCGGTACTCTCACTCTATCTCTCATTCTCTTTTTGTGTGTGTGTGTGTGATGGTTTTATGCTTTGTAGAATACATGATCACTATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006323 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0006997 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007289 GO:0007399 GO:0007417 GO:0007420 GO:0008150 GO:0008152 GO:0008213 GO:0008285 GO:0009506 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016581 GO:0016604 GO:0016607 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0021895 GO:0021953 GO:0022008 GO:0022412 GO:0022414 GO:0023051 GO:0023056 GO:0030054 GO:0030154 GO:0030182 GO:0030900 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034728 GO:0034968 GO:0035064 GO:0035092 GO:0035093 GO:0036211 GO:0042127 GO:0042393 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043486 GO:0043543 GO:0043933 GO:0043967 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0045165 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048609 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0051704 GO:0055044 GO:0060255 GO:0060322 GO:0060850 GO:0061628 GO:0065007 GO:0070013 GO:0070603 GO:0070734 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0098532 GO:0140030 GO:0140034 GO:1901564 GO:1901796 GO:1901798 GO:1902494 GO:1902531 GO:1902533 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 ko05203 map05165 map05203
Pfam Domains
Protein Families
Chromo DUF1087 Helicase_C PHD SNF2_N

Protein Analysis

1761

Amino Acids

195.52

Weight (kDa)

6.09

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 79 - 120 2.9e-09 PHD-finger
Chromo PF00385 736 - 786 1.7e-08 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 829 - 1000 3.3e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 832 - 1121 2.1e-79 SNF2-related domain
HDA2-3 PF11496 1069 - 1286 9.6e-12 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 1145 - 1259 9.3e-23 Helicase conserved C-terminal domain
DUF1087 PF06465 1440 - 1481 1.7e-06 CHD subfamily II, DUF1087
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2180, 2817
AarI CACCTGC 2 cut(s) 1402, 2018
AasI GACNNNNNNGTC 1 cut(s) 4356
Acc36I ACCTGC 3 cut(s) 94, 1402, 2018
AccB1I GGYRCC 1 cut(s) 2993
AccB7I CCANNNNNTGG 2 cut(s) 2377, 5180
AccBSI CCGCTC 1 cut(s) 4525
AccI GTMKAC 1 cut(s) 2938
AccII CGCG 4 cut(s) 1024, 3619, 4880, 5141
AccIII TCCGGA 3 cut(s) 784, 874, 1852
AclI AACGTT 1 cut(s) 2375
AcoI YGGCCR 1 cut(s) 3540
AcsI RAATTY 9 cut(s) 1437, 1549, 1911, 2057, 2329, 2819, 2926, 3548, 5054
AcuI CTGAAG 6 cut(s) 477, 1005, 2987, 3090, 3411, 4734
AfaI GTAC 8 cut(s) 28, 1691, 2982, 3026, 4078, 4327, 4532, 5202
AfiI CCNNNNNNNGG 9 cut(s) 1315, 1414, 2377, 3386, 4637, 4915, 4967, 5081, 5180
AflII CTTAAG 4 cut(s) 320, 3458, 3920, 4810
AflIII ACRYGT 1 cut(s) 4878
AgeI ACCGGT 1 cut(s) 5197
AhdI GACNNNNNGTC 2 cut(s) 3388, 4879
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
AjuI GAANNNNNNNTTGG 2 cut(s) 4383, 4415
AleI CACNNNNGTG 1 cut(s) 4461
Alw21I GWGCWC 5 cut(s) 728, 2370, 3766, 4288, 4552
Alw26I GTCTC 5 cut(s) 761, 1522, 2036, 2088, 4887
AlwNI CAGNNNCTG 6 cut(s) 45, 1495, 1970, 2341, 3374, 3596
Aor13HI TCCGGA 3 cut(s) 784, 874, 1852
AoxI GGCC 4 cut(s) 2700, 2911, 3540, 3673
ApaI GGGCCC 1 cut(s) 2704
ApoI RAATTY 9 cut(s) 1437, 1549, 1911, 2057, 2329, 2819, 2926, 3548, 5054
ArsI GACNNNNNNTTYG 2 cut(s) 4579, 4611
AseI ATTAAT 1 cut(s) 1376
AsiGI ACCGGT 1 cut(s) 5197
Asp700I GAANNNNTTC 5 cut(s) 575, 1902, 2850, 3915, 4143
AspLEI GCGC 3 cut(s) 1116, 3619, 3834
AsuHPI GGTGA 7 cut(s) 2042, 2230, 2264, 3029, 3992, 4248, 5123
AsuII TTCGAA 1 cut(s) 564
AvaII GGWCC 7 cut(s) 83, 1306, 1492, 2881, 3554, 5048, 5085
AxyI CCTNAGG 2 cut(s) 906, 966
BaeGI GKGCMC 3 cut(s) 353, 2704, 4981
BalI TGGCCA 1 cut(s) 3542
BamHI GGATCC 1 cut(s) 1765
BanI GGYRCC 1 cut(s) 2993
BanII GRGCYC 4 cut(s) 728, 2704, 4509, 4552
BarI GAAGNNNNNNTAC 2 cut(s) 3387, 3419
BbsI GAAGAC 5 cut(s) 114, 146, 2810, 3063, 4740
Bbv12I GWGCWC 5 cut(s) 728, 2370, 3766, 4288, 4552
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 6 cut(s) 1400, 1434, 3291, 3325, 4831, 4865
BciT130I CCWGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
BclI TGATCA 3 cut(s) 1783, 4693, 5272
BcoDI GTCTC 5 cut(s) 761, 1522, 2036, 2088, 4887
BfmI CTRYAG 3 cut(s) 1409, 1770, 3393
BfrI CTTAAG 4 cut(s) 320, 3458, 3920, 4810
BfuAI ACCTGC 3 cut(s) 94, 1402, 2018
BglI GCCNNNNNGGC 1 cut(s) 2677
BglII AGATCT 1 cut(s) 680
BlpI GCTNAGC 1 cut(s) 4569
Bme1390I CCNGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
Bme18I GGWCC 7 cut(s) 83, 1306, 1492, 2881, 3554, 5048, 5085
BmeRI GACNNNNNGTC 2 cut(s) 3388, 4879
BmgBI CACGTC 1 cut(s) 376
BmrFI CCNGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
BmrI ACTGGG 3 cut(s) 55, 606, 4713
BmuI ACTGGG 3 cut(s) 55, 606, 4713
BoxI GACNNNNGTC 2 cut(s) 196, 3636
BpiI GAAGAC 5 cut(s) 114, 146, 2810, 3063, 4740
BplI GAGNNNNNCTC 4 cut(s) 2317, 2349, 3209, 3241
BpmI CTGGAG 8 cut(s) 284, 714, 798, 825, 855, 866, 885, 915
Bpu1102I GCTNAGC 1 cut(s) 4569
Bpu14I TTCGAA 1 cut(s) 564
BpuEI CTTGAG 4 cut(s) 117, 1165, 3548, 5002
Bsa29I ATCGAT 2 cut(s) 2917, 3311
BsaBI GATNNNNATC 2 cut(s) 1788, 4692
BsaWI WCCGGW 4 cut(s) 784, 874, 1852, 5197
BsaXI ACNNNNNCTCC 2 cut(s) 4789, 4819
Bsc4I CCNNNNNNNGG 9 cut(s) 1315, 1414, 2377, 3386, 4637, 4915, 4967, 5081, 5180
Bse118I RCCGGY 1 cut(s) 5197
Bse21I CCTNAGG 2 cut(s) 906, 966
Bse3DI GCAATG 1 cut(s) 3258
Bse8I GATNNNNATC 2 cut(s) 1788, 4692
BseAI TCCGGA 3 cut(s) 784, 874, 1852
BseBI CCWGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
BseCI ATCGAT 2 cut(s) 2917, 3311
BseJI GATNNNNATC 2 cut(s) 1788, 4692
BseLI CCNNNNNNNGG 9 cut(s) 1315, 1414, 2377, 3386, 4637, 4915, 4967, 5081, 5180
BseMI GCAATG 1 cut(s) 3258
BseRI GAGGAG 6 cut(s) 2207, 3095, 4241, 4400, 5055, 5064
BseSI GKGCMC 3 cut(s) 353, 2704, 4981
BseYI CCCAGC 1 cut(s) 2100
BsgI GTGCAG 2 cut(s) 2046, 4437
Bsh1236I CGCG 4 cut(s) 1024, 3619, 4880, 5141
BshFI GGCC 4 cut(s) 2702, 2913, 3542, 3675
BshNI GGYRCC 1 cut(s) 2993
BshTI ACCGGT 1 cut(s) 5197
BshVI ATCGAT 2 cut(s) 2917, 3311
BsiHKAI GWGCWC 5 cut(s) 728, 2370, 3766, 4288, 4552
BsiSI CCGG 5 cut(s) 785, 875, 1353, 1853, 5198
BslFI GGGAC 8 cut(s) 267, 1204, 2867, 4358, 4653, 4653, 4983, 5098
BslI CCNNNNNNNGG 9 cut(s) 1315, 1414, 2377, 3386, 4637, 4915, 4967, 5081, 5180
BsmAI GTCTC 5 cut(s) 761, 1522, 2036, 2088, 4887
BsmBI CGTCTC 2 cut(s) 2036, 4887
BsmFI GGGAC 8 cut(s) 267, 1204, 2867, 4358, 4653, 4653, 4983, 5098
BsmI GAATGC 3 cut(s) 2297, 3448, 4422
BsnI GGCC 4 cut(s) 2702, 2913, 3542, 3675
Bsp119I TTCGAA 1 cut(s) 564
Bsp120I GGGCCC 1 cut(s) 2700
Bsp1286I GDGCHC 9 cut(s) 353, 728, 2370, 2704, 3766, 4288, 4509, 4552, 4981
Bsp13I TCCGGA 3 cut(s) 784, 874, 1852
Bsp1407I TGTACA 2 cut(s) 1689, 3024
Bsp1720I GCTNAGC 1 cut(s) 4569
Bsp19I CCATGG 3 cut(s) 1234, 1731, 2728
BspANI GGCC 4 cut(s) 2702, 2913, 3542, 3675
BspDI ATCGAT 2 cut(s) 2917, 3311
BspEI TCCGGA 3 cut(s) 784, 874, 1852
BspFNI CGCG 4 cut(s) 1024, 3619, 4880, 5141
BspHI TCATGA 2 cut(s) 1882, 3406
BspMAI CTGCAG 2 cut(s) 1413, 1774
BspMI ACCTGC 3 cut(s) 94, 1402, 2018
BspQI GCTCTTC 2 cut(s) 3822, 4233
BspT104I TTCGAA 1 cut(s) 564
BspT107I GGYRCC 1 cut(s) 2993
BspTI CTTAAG 4 cut(s) 320, 3458, 3920, 4810
BsrBI CCGCTC 1 cut(s) 4525
BsrDI GCAATG 1 cut(s) 3258
BsrFI RCCGGY 1 cut(s) 5197
BsrGI TGTACA 2 cut(s) 1689, 3024
BssAI RCCGGY 1 cut(s) 5197
BssT1I CCWWGG 8 cut(s) 1234, 1275, 1731, 1755, 2463, 2728, 2885, 3676
Bst2UI CCWGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
Bst6I CTCTTC 8 cut(s) 1094, 1121, 1893, 3006, 3822, 4031, 4233, 5156
BstAFI CTTAAG 4 cut(s) 320, 3458, 3920, 4810
BstAPI GCANNNNNTGC 3 cut(s) 3454, 4451, 4565
BstAUI TGTACA 2 cut(s) 1689, 3024
BstBI TTCGAA 1 cut(s) 564
BstDSI CCRYGG 5 cut(s) 1234, 1731, 2230, 2728, 5139
BstENI CCTNNNNNAGG 1 cut(s) 1313
BstFNI CGCG 4 cut(s) 1024, 3619, 4880, 5141
BstHHI GCGC 3 cut(s) 1116, 3619, 3834
BstMAI GTCTC 5 cut(s) 761, 1522, 2036, 2088, 4887
BstNI CCWGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
BstNSI RCATGY 4 cut(s) 1688, 1928, 2777, 3415
BstPAI GACNNNNGTC 2 cut(s) 196, 3636
BstSCI CCNGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
BstSFI CTRYAG 3 cut(s) 1409, 1770, 3393
BstSLI GKGCMC 3 cut(s) 353, 2704, 4981
BstUI CGCG 4 cut(s) 1024, 3619, 4880, 5141
BstV2I GAAGAC 5 cut(s) 114, 146, 2810, 3063, 4740
BstX2I RGATCY 5 cut(s) 680, 1163, 1765, 2005, 3581
BstXI CCANNNNNNTGG 1 cut(s) 1762
BstYI RGATCY 5 cut(s) 680, 1163, 1765, 2005, 3581
Bsu15I ATCGAT 2 cut(s) 2917, 3311
Bsu36I CCTNAGG 2 cut(s) 906, 966
BsuRI GGCC 4 cut(s) 2702, 2913, 3542, 3675
BsuTUI ATCGAT 2 cut(s) 2917, 3311
BtgI CCRYGG 5 cut(s) 1234, 1731, 2230, 2728, 5139
BtgZI GCGATG 1 cut(s) 684
BtrI CACGTC 1 cut(s) 376
BtsI GCAGTG 4 cut(s) 353, 3597, 4014, 4677
BveI ACCTGC 3 cut(s) 94, 1402, 2018
CaiI CAGNNNCTG 6 cut(s) 45, 1495, 1970, 2341, 3374, 3596
CciI TCATGA 2 cut(s) 1882, 3406
CfoI GCGC 3 cut(s) 1116, 3619, 3834
Cfr10I RCCGGY 1 cut(s) 5197
Cfr42I CCGCGG 1 cut(s) 5142
ClaI ATCGAT 2 cut(s) 2917, 3311
CseI GACGC 1 cut(s) 4869
Csp6I GTAC 8 cut(s) 27, 1690, 2981, 3025, 4077, 4326, 4531, 5201
CspAI ACCGGT 1 cut(s) 5197
CviQI GTAC 8 cut(s) 27, 1690, 2981, 3025, 4077, 4326, 4531, 5201
DraI TTTAAA 4 cut(s) 390, 1809, 2239, 4122
DrdI GACNNNNNNGTC 1 cut(s) 4356
DriI GACNNNNNGTC 2 cut(s) 3388, 4879
DseDI GACNNNNNNGTC 1 cut(s) 4356
EaeI YGGCCR 1 cut(s) 3540
Eam1104I CTCTTC 8 cut(s) 1094, 1121, 1893, 3006, 3822, 4031, 4233, 5156
Eam1105I GACNNNNNGTC 2 cut(s) 3388, 4879
EarI CTCTTC 8 cut(s) 1094, 1121, 1893, 3006, 3822, 4031, 4233, 5156
Ecl136II GAGCTC 2 cut(s) 726, 4550
Eco130I CCWWGG 8 cut(s) 1234, 1275, 1731, 1755, 2463, 2728, 2885, 3676
Eco24I GRGCYC 4 cut(s) 728, 2704, 4509, 4552
Eco32I GATATC 2 cut(s) 1981, 3523
Eco47I GGWCC 7 cut(s) 83, 1306, 1492, 2881, 3554, 5048, 5085
Eco53kI GAGCTC 2 cut(s) 726, 4550
Eco57I CTGAAG 6 cut(s) 477, 1005, 2987, 3090, 3411, 4734
Eco81I CCTNAGG 2 cut(s) 906, 966
EcoICRI GAGCTC 2 cut(s) 726, 4550
EcoNI CCTNNNNNAGG 1 cut(s) 1313
EcoO109I RGGNCCY 5 cut(s) 1306, 1492, 2701, 2881, 5085
EcoRI GAATTC 1 cut(s) 1437
EcoRII CCWGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
EcoRV GATATC 2 cut(s) 1981, 3523
EcoT14I CCWWGG 8 cut(s) 1234, 1275, 1731, 1755, 2463, 2728, 2885, 3676
EcoT22I ATGCAT 3 cut(s) 943, 1018, 5137
EcoT38I GRGCYC 4 cut(s) 728, 2704, 4509, 4552
ErhI CCWWGG 8 cut(s) 1234, 1275, 1731, 1755, 2463, 2728, 2885, 3676
Esp3I CGTCTC 2 cut(s) 2036, 4887
FalI AAGNNNNNCTT 4 cut(s) 3903, 3935, 4383, 4415
FaqI GGGAC 8 cut(s) 267, 1204, 2867, 4358, 4653, 4653, 4983, 5098
FauI CCCGC 3 cut(s) 2208, 4518, 4546
FauNDI CATATG 4 cut(s) 2842, 3147, 3565, 4447
FbaI TGATCA 3 cut(s) 1783, 4693, 5272
FblI GTMKAC 1 cut(s) 2938
FriOI GRGCYC 4 cut(s) 728, 2704, 4509, 4552
GlaI GCGC 3 cut(s) 1115, 3618, 3833
GsaI CCCAGC 1 cut(s) 2104
GsuI CTGGAG 8 cut(s) 284, 714, 798, 825, 855, 866, 885, 915
HaeIII GGCC 4 cut(s) 2702, 2913, 3542, 3675
HapII CCGG 5 cut(s) 785, 875, 1353, 1853, 5198
HgaI GACGC 1 cut(s) 4869
HhaI GCGC 3 cut(s) 1116, 3619, 3834
Hin6I GCGC 3 cut(s) 1114, 3617, 3832
HinP1I GCGC 3 cut(s) 1114, 3617, 3832
HincII GTYRAC 4 cut(s) 21, 2988, 3258, 3438
HindII GTYRAC 4 cut(s) 21, 2988, 3258, 3438
HindIII AAGCTT 4 cut(s) 71, 2256, 3423, 3857
HpaI GTTAAC 1 cut(s) 3258
HpaII CCGG 5 cut(s) 785, 875, 1353, 1853, 5198
HphI GGTGA 7 cut(s) 2042, 2230, 2264, 3029, 3992, 4248, 5123
Hpy166II GTNNAC 9 cut(s) 21, 1675, 1690, 2939, 2981, 2988, 3258, 3438, 4533
Hpy8I GTNNAC 9 cut(s) 21, 1675, 1690, 2939, 2981, 2988, 3258, 3438, 4533
Hpy99I CGWCG 1 cut(s) 165
HpyCH4IV ACGT 7 cut(s) 64, 375, 2023, 2045, 2375, 4357, 4594
HpySE526I ACGT 7 cut(s) 64, 375, 2023, 2045, 2375, 4357, 4594
HspAI GCGC 3 cut(s) 1114, 3617, 3832
KflI GGGWCCC 1 cut(s) 2881
Kpn2I TCCGGA 3 cut(s) 784, 874, 1852
Ksp22I TGATCA 3 cut(s) 1783, 4693, 5272
KspAI GTTAAC 1 cut(s) 3258
KspI CCGCGG 1 cut(s) 5142
LguI GCTCTTC 2 cut(s) 3822, 4233
MaeII ACGT 7 cut(s) 64, 375, 2023, 2045, 2375, 4357, 4594
MbiI CCGCTC 1 cut(s) 4525
MfeI CAATTG 3 cut(s) 2512, 3612, 4233
MflI RGATCY 5 cut(s) 680, 1163, 1765, 2005, 3581
MhlI GDGCHC 9 cut(s) 353, 728, 2370, 2704, 3766, 4288, 4509, 4552, 4981
MlsI TGGCCA 1 cut(s) 3542
MluI ACGCGT 1 cut(s) 4878
MluNI TGGCCA 1 cut(s) 3542
MlyI GAGTC 8 cut(s) 1186, 1374, 1843, 3495, 4344, 4661, 4796, 4924
MmeI TCCRAC 2 cut(s) 1743, 2995
Mox20I TGGCCA 1 cut(s) 3542
Mph1103I ATGCAT 3 cut(s) 943, 1018, 5137
MroI TCCGGA 3 cut(s) 784, 874, 1852
MroXI GAANNNNTTC 5 cut(s) 575, 1902, 2850, 3915, 4143
MscI TGGCCA 1 cut(s) 3542
MslI CAYNNNNRTG 5 cut(s) 2733, 2778, 2874, 3326, 4461
Msp20I TGGCCA 1 cut(s) 3542
MspA1I CMGCKG 8 cut(s) 626, 794, 824, 854, 884, 1484, 2215, 5141
MspCI CTTAAG 4 cut(s) 320, 3458, 3920, 4810
MspI CCGG 5 cut(s) 785, 875, 1353, 1853, 5198
MspR9I CCNGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
MunI CAATTG 3 cut(s) 2512, 3612, 4233
Mva1269I GAATGC 3 cut(s) 2297, 3448, 4422
MvaI CCWGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
MvnI CGCG 4 cut(s) 1024, 3619, 4880, 5141
NcoI CCATGG 3 cut(s) 1234, 1731, 2728
NdeI CATATG 4 cut(s) 2842, 3147, 3565, 4447
NsiI ATGCAT 3 cut(s) 943, 1018, 5137
NspI RCATGY 4 cut(s) 1688, 1928, 2777, 3415
NspV TTCGAA 1 cut(s) 564
OliI CACNNNNGTG 1 cut(s) 4461
PaeI GCATGC 1 cut(s) 2777
PagI TCATGA 2 cut(s) 1882, 3406
PaqCI CACCTGC 2 cut(s) 1402, 2018
PasI CCCWGGG 1 cut(s) 1187
PciSI GCTCTTC 2 cut(s) 3822, 4233
PcsI WCGNNNNNNNCGW 3 cut(s) 1794, 3766, 4411
PctI GAATGC 3 cut(s) 2297, 3448, 4422
PdmI GAANNNNTTC 5 cut(s) 575, 1902, 2850, 3915, 4143
PflFI GACNNNGTC 1 cut(s) 3704
PflMI CCANNNNNTGG 2 cut(s) 2377, 5180
PinAI ACCGGT 1 cut(s) 5197
PleI GAGTC 8 cut(s) 1186, 1373, 1843, 3494, 4344, 4660, 4796, 4924
PpsI GAGTC 8 cut(s) 1186, 1373, 1843, 3494, 4344, 4660, 4796, 4924
PpuMI RGGWCCY 4 cut(s) 1306, 1492, 2881, 5085
PshAI GACNNNNGTC 2 cut(s) 196, 3636
PshBI ATTAAT 1 cut(s) 1376
PsiI TTATAA 2 cut(s) 2180, 2817
Psp124BI GAGCTC 2 cut(s) 728, 4552
Psp1406I AACGTT 1 cut(s) 2375
Psp5II RGGWCCY 4 cut(s) 1306, 1492, 2881, 5085
Psp6I CCWGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
PspFI CCCAGC 1 cut(s) 2100
PspGI CCWGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
PspOMI GGGCCC 1 cut(s) 2700
PspPPI RGGWCCY 4 cut(s) 1306, 1492, 2881, 5085
PstI CTGCAG 2 cut(s) 1413, 1774
PstNI CAGNNNCTG 6 cut(s) 45, 1495, 1970, 2341, 3374, 3596
PsuI RGATCY 5 cut(s) 680, 1163, 1765, 2005, 3581
PsyI GACNNNGTC 1 cut(s) 3704
PvuII CAGCTG 6 cut(s) 626, 794, 824, 854, 884, 1484
RsaI GTAC 8 cut(s) 28, 1691, 2982, 3026, 4078, 4327, 4532, 5202
RsaNI GTAC 8 cut(s) 27, 1690, 2981, 3025, 4077, 4326, 4531, 5201
RseI CAYNNNNRTG 5 cut(s) 2733, 2778, 2874, 3326, 4461
SacI GAGCTC 2 cut(s) 728, 4552
SacII CCGCGG 1 cut(s) 5142
SapI GCTCTTC 2 cut(s) 3822, 4233
SbfI CCTGCAGG 2 cut(s) 1413, 1774
SchI GAGTC 8 cut(s) 1186, 1374, 1843, 3495, 4344, 4661, 4796, 4924
ScrFI CCNGG 7 cut(s) 1188, 1490, 2129, 3369, 3957, 4052, 4263
SdaI CCTGCAGG 2 cut(s) 1413, 1774
SduI GDGCHC 9 cut(s) 353, 728, 2370, 2704, 3766, 4288, 4509, 4552, 4981
SfcI CTRYAG 3 cut(s) 1409, 1770, 3393
Sfr303I CCGCGG 1 cut(s) 5142
SfuI TTCGAA 1 cut(s) 564
SgrBI CCGCGG 1 cut(s) 5142
SinI GGWCC 7 cut(s) 83, 1306, 1492, 2881, 3554, 5048, 5085
SmiMI CAYNNNNRTG 5 cut(s) 2733, 2778, 2874, 3326, 4461
SmlI CTYRAG 8 cut(s) 132, 320, 1180, 3458, 3527, 3920, 4810, 5017
SmoI CTYRAG 8 cut(s) 132, 320, 1180, 3458, 3527, 3920, 4810, 5017
SphI GCATGC 1 cut(s) 2777
Sse8387I CCTGCAGG 2 cut(s) 1413, 1774
SspI AATATT 3 cut(s) 1861, 3184, 3286
SstI GAGCTC 2 cut(s) 728, 4552
StyD4I CCNGG 7 cut(s) 1186, 1488, 2127, 3367, 3955, 4050, 4261
StyI CCWWGG 8 cut(s) 1234, 1275, 1731, 1755, 2463, 2728, 2885, 3676
TaiI ACGT 7 cut(s) 67, 378, 2026, 2048, 2378, 4360, 4597
TaqII GACCGA 1 cut(s) 3212
TatI WGTACW 5 cut(s) 26, 1689, 2980, 3024, 4530
TauI GCSGC 2 cut(s) 1586, 4718
TspGWI ACGGA 4 cut(s) 2219, 4094, 4565, 5050
Tth111I GACNNNGTC 1 cut(s) 3704
Van91I CCANNNNNTGG 2 cut(s) 2377, 5180
Vha464I CTTAAG 4 cut(s) 320, 3458, 3920, 4810
VpaK11BI GGWCC 7 cut(s) 83, 1306, 1492, 2881, 3554, 5048, 5085
VspI ATTAAT 1 cut(s) 1376
XagI CCTNNNNNAGG 1 cut(s) 1313
XapI RAATTY 9 cut(s) 1437, 1549, 1911, 2057, 2329, 2819, 2926, 3548, 5054
XceI RCATGY 4 cut(s) 1688, 1928, 2777, 3415
XmiI GTMKAC 1 cut(s) 2938
XmnI GAANNNNTTC 5 cut(s) 575, 1902, 2850, 3915, 4143
Zsp2I ATGCAT 3 cut(s) 943, 1018, 5137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.