Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003307

Physical Location & Seq

Genomic Coordinates Forward (+)

25118032 .. 25122046

Gene Structure 4015 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1113000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGCTGGCTCGTGGAGAGCTCGAGGATCTCTCGTCGTGCTTGCGATCGTCACATTCGGGTGCCTTTTTGCAATTTCCATCGCGAAAGAAGAAGCGACCAAGTTGGGAACAGTCATTGGAATTGATCTTGGAACCACTTATTCCTGTGTTGGTGTTTACAAGAATGGTCACGTGGAAATTATAGCCAATGACCAGGGTAACCGTATCACCCCTTCATGGGTGGCTTTCTCTGACAGTGAGAGACTGATCGGTGAGGCTGCTAAGAACCAGGCAGCTGTGAACCCTGAAAGGACTGTCTTTGATGTCAAGAGACTTATCGGACGAAAGTTTGAGGACAAGGAAGTTCAGAAAGACATGAAGCTTGTTCCTTACAAGATTGTTAACAGAGATGGAAAGCCTTATATCCAAGTGAGGATCAAGGATGGTGAGACCAAGGTTTTCAGCCCCGAAGAGGTCAGCGCCATGATTTTGACTAAGATGAAGGAAACAGCCGAAGCATTCCTTGGGAAGAAAATCAAGGATGCCGTCGTTACTGTTCCTGCATACTTCAATGATGCCCAAAGACAGGCAACCAAGGATGCCGGTGTGATTGCTGGGCTGAATGTTGCTAGGATTATCAATGAACCAACTGCAGCCGCCATCGCATATGGTTTGGACAAGAAGGGCGGTGAAAAGAACATCCTTGTCTTTGACCTTGGTGGTGGAACTTTTGATGTCAGTATTTTGACAATTGATAATGGTGTTTTCGAGGTTCTTTCCACAAATGGAGACACTCATTTGGGAGGTGAGGACTTTGACCACAGGATCATGGAGTACTTCATCAAGTTGATTAAGAAGAAGCACGGAAAGGACATCAGCAAGGACAACAGAGCCCTTGGAAAACTCAGGAGGGAAGCCGAGCGTGCCAAGAGAGCACTCAGTAGCCAGCACCAAGTCCGTGTGGAGATTGAATCCCTTTTTGATGGAGTGGACTTCTCTGAACCCCTCACCAGAGCTAGATTCGAAGAGTTGAACAATGACTTGTTCAGAAAGACCATGGGACCTGTGAAGAAGGCTATGGACGACGCTGGCTTGGAGAAGCGCCAGATTGACGAGATTGTGCTTGTTGGTGGAAGTACCAGGATTCCCAAGGTCCAACAGCTTCTTAAGGACTACTTTGATGGCAAGGAGCCAAACAAGGGTGTGAACCCTGATGAGGCTGTTGCTTATGGTGCCGCTGTACAAGGAAGCATCTTGAGTGGAGAGGGTGGTGAAGAAACCAAAGATATTCTTCTCCTTGACGTCGCACCTCTCACCCTCGGTATTGAAACTGTTGGAGGAGTGATGACCAAGCTTATCCCAAGAAACACCGTGATCCCAACCAAGAAATCCCAGGTTTTCACCACCTACCAGGATCAGCAGACAACCGTCTCCATTCAGGTCTTCGAGGGTGAAAGGAGTCTCACAAAGGATTGCAGGAACCTTGGTAAATTTGATCTTACCGGAATTCCTCCAGCTCCAAGGGGAACTCCTCAAATCGAAGTCACATTCGAAGTGGATGCCAACGGTATCCTGAACGTCAGGGCAGAAGACAAGGGCACAGGCAAATCCGAGAAGATCACCATCACAAACGACAAGGGCCGTCTCAGCCAGGAGGAGATTGACCGCATGGTTAAGGAGGCAGAAGAGTTTGCCGAGGAAGACAAGAAGGTGAAGGAGAGAATCGATGCCCGCAACAGCCTCGAGACCTATGTCTACAACATGAAGAACCAGATCAATGACAAAGACAAGCTTGCTGACAAGCTTGAGTCCGATGAGAAGGAAAAGATTGAGACCGCGACAAAGGAAGCCCTCGAGTGGTTGGATGACAACCAGACCGCCGAGAAGGAAGACTATGACGAGAAGCTCAAGGAGGTTGAAGCCGTGTGCAACCCCATAATCTCAGCCGTCTACCAGAGGTCAGGAGGTGCCCCGGGTGGTGCCGGAGCCTCGGAGGAGGACGATGAGTCACATGACGAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0012505 GO:0031974 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

668

Amino Acids

73.58

Weight (kDa)

5.17

Isoelectric Point (pI)

27.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	38 - 645	5.7e-265	Hsp70 protein
MreB_Mbl	PF06723	153 - 405	1.6e-15	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28540 AT5G42020 AT5G42020 AT5G42020
fragaria_vesca	FvH4_3g14480 FvH4_5g11500 FvH4_5g11500
malus_domestica	MD06G1113000.v1.1 MD14G1134100.v1.1
prunus_persica	Prupe.5G125900_v2.0.a1 Prupe.5G125900_v2.0.a1 Prupe.5G125900_v2.0.a1
pyrus_communis	pycom06g10560 pycom06g10570
rosa_chinensis	RchiOBHm_Chr5g0023851 RchiOBHm_Chr7g0186301
rosa_laevigata	RLG00000004851 RLG00000032740
rosa_multiflora	Rmu_co8308791.1_g000001 Rmu_sc0001372.1_g000006 Rmu_sc0018092.1_g000001 Rmu_ssc0000027.1_g000008
rosa_roxburghii	Rroxscaffold_1G00055390 Rroxscaffold_3G00268240
rosa_rugosa	Rorug05G0079800 Rorug06G0473100
rosa_samantha	Rh5AG169800 Rh5BG167100 Rh5CG182700 Rh5DG169200 Rh7AG067900 Rh7BG083700 Rh7CG080100 Rh7DG080700
rosa_wichuraiana	Rw5G015350 Rw7G006710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1284
AccB1I	GGYRCC	4 cut(s)	60, 1211, 1946, 1958
AccI	GTMKAC	2 cut(s)	1734, 1929
AccII	CGCG	2 cut(s)	83, 1817
AciI	CCGC	7 cut(s)	636, 666, 1215, 1645, 1711, 1815, 1857
AclWI	GGATC	5 cut(s)	34, 422, 812, 1348, 1401
AcsI	RAATTY	2 cut(s)	1469, 1485
AcvI	CACGTG	1 cut(s)	172
AcyI	GRCGYC	1 cut(s)	1281
AfaI	GTAC	3 cut(s)	815, 1117, 1221
AfiI	CCNNNNNNNGG	7 cut(s)	216, 217, 451, 565, 1178, 1195, 1836
AflII	CTTAAG	1 cut(s)	1145
AgsI	TTSAA	5 cut(s)	550, 950, 1012, 1307, 1898
AhdI	GACNNNNNGTC	1 cut(s)	1984
AjnI	CCWGG	6 cut(s)	192, 267, 1118, 1371, 1389, 1629
AjuI	GAANNNNNNNTTGG	2 cut(s)	486, 518
Alw21I	GWGCWC	2 cut(s)	22, 916
Alw26I	GTCTC	9 cut(s)	235, 304, 422, 762, 1414, 1445, 1628, 1718, 1805
AlwI	GGATC	5 cut(s)	34, 422, 812, 1348, 1401
Ama87I	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
AoxI	GGCC	1 cut(s)	1618
ApeKI	GCWGC	3 cut(s)	257, 272, 632
ApoI	RAATTY	2 cut(s)	1469, 1485
AspLEI	GCGC	2 cut(s)	461, 1083
AspS9I	GGNCC	3 cut(s)	1040, 1132, 1618
AsuC2I	CCSGG	2 cut(s)	1952, 1953
AsuII	TTCGAA	2 cut(s)	1002, 1530
AvaI	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
AvaII	GGWCC	2 cut(s)	1040, 1132
BaeGI	GKGCMC	2 cut(s)	1580, 1951
BanI	GGYRCC	4 cut(s)	60, 1211, 1946, 1958
BanII	GRGCYC	2 cut(s)	22, 874
BarI	GAAGNNNNNNTAC	2 cut(s)	196, 228
BauI	CACGAG	1 cut(s)	10
BbrPI	CACGTG	1 cut(s)	172
BbsI	GAAGAC	4 cut(s)	1414, 1575, 1686, 1875
Bbv12I	GWGCWC	2 cut(s)	22, 916
BbvI	GCAGC	3 cut(s)	244, 284, 644
BccI	CCATC	7 cut(s)	86, 383, 416, 647, 956, 1154, 1610
BceAI	ACGGC	4 cut(s)	509, 1605, 1886, 1910
BcgI	CGANNNNNNTGC	4 cut(s)	1520, 1554, 1702, 1736
BciT130I	CCWGG	6 cut(s)	194, 269, 1120, 1373, 1391, 1631
BciVI	GTATCC	1 cut(s)	1559
BcnI	CCSGG	2 cut(s)	1952, 1953
BcoDI	GTCTC	9 cut(s)	235, 304, 422, 762, 1414, 1445, 1628, 1718, 1805
BfaI	CTAG	2 cut(s)	609, 996
BfmI	CTRYAG	1 cut(s)	630
BfoI	RGCGCY	2 cut(s)	462, 1084
BfrI	CTTAAG	1 cut(s)	1145
BfuI	GTATCC	1 cut(s)	1559
BisI	GCNGC	5 cut(s)	258, 273, 633, 636, 1215
BlsI	GCNGC	5 cut(s)	259, 274, 634, 637, 1216
BmcAI	AGTACT	1 cut(s)	815
Bme1390I	CCNGG	8 cut(s)	194, 269, 1120, 1373, 1391, 1631, 1952, 1953
Bme18I	GGWCC	2 cut(s)	1040, 1132
BmeRI	GACNNNNNGTC	1 cut(s)	1984
BmeT110I	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
BmgT120I	GGNCC	3 cut(s)	1040, 1132, 1618
BmiI	GGNNCC	9 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1960, 1966
BmrFI	CCNGG	8 cut(s)	194, 269, 1120, 1373, 1391, 1631, 1952, 1953
BmsI	GCATC	6 cut(s)	511, 544, 568, 1239, 1528, 1696
BoxI	GACNNNNGTC	2 cut(s)	1406, 1730
BpiI	GAAGAC	4 cut(s)	1414, 1575, 1686, 1875
BplI	GAGNNNNNCTC	2 cut(s)	15, 47
BpmI	CTGGAG	1 cut(s)	1476
Bpu14I	TTCGAA	2 cut(s)	1002, 1530
BpuEI	CTTGAG	3 cut(s)	1255, 1805, 1871
BpuMI	CCSGG	2 cut(s)	1952, 1953
Bsa29I	ATCGAT	1 cut(s)	1704
BsaAI	YACGTR	1 cut(s)	172
BsaBI	GATNNNNATC	1 cut(s)	1601
BsaHI	GRCGYC	1 cut(s)	1281
BsaI	GGTCTC	3 cut(s)	422, 1718, 1805
BsaWI	WCCGGW	1 cut(s)	1481
Bsc4I	CCNNNNNNNGG	7 cut(s)	216, 217, 451, 565, 1178, 1195, 1836
Bse118I	RCCGGY	1 cut(s)	581
Bse8I	GATNNNNATC	1 cut(s)	1601
BseBI	CCWGG	6 cut(s)	194, 269, 1120, 1373, 1391, 1631
BseCI	ATCGAT	1 cut(s)	1704
BseGI	GGATG	6 cut(s)	427, 526, 583, 678, 1543, 1849
BseJI	GATNNNNATC	1 cut(s)	1601
BseLI	CCNNNNNNNGG	7 cut(s)	216, 217, 451, 565, 1178, 1195, 1836
BseMII	CTCAG	4 cut(s)	898, 931, 1639, 1935
BseRI	GAGGAG	4 cut(s)	1332, 1500, 1649, 1988
BseSI	GKGCMC	2 cut(s)	1580, 1951
BseXI	GCAGC	3 cut(s)	244, 284, 644
BseYI	CCCAGC	1 cut(s)	593
Bsh1236I	CGCG	2 cut(s)	83, 1817
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	1 cut(s)	1620
BshNI	GGYRCC	4 cut(s)	60, 1211, 1946, 1958
BshVI	ATCGAT	1 cut(s)	1704
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	2 cut(s)	22, 916
BsiHKCI	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
BsiSI	CCGG	4 cut(s)	582, 1482, 1952, 1962
BslFI	GGGAC	1 cut(s)	1053
BslI	CCNNNNNNNGG	7 cut(s)	216, 217, 451, 565, 1178, 1195, 1836
BsmAI	GTCTC	9 cut(s)	235, 304, 422, 762, 1414, 1445, 1628, 1718, 1805
BsmBI	CGTCTC	2 cut(s)	1414, 1628
BsmFI	GGGAC	1 cut(s)	1053
BsmI	GAATGC	1 cut(s)	497
BsnI	GGCC	1 cut(s)	1620
Bso31I	GGTCTC	3 cut(s)	422, 1718, 1805
BsoBI	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
Bsp119I	TTCGAA	2 cut(s)	1002, 1530
Bsp1286I	GDGCHC	5 cut(s)	22, 874, 916, 1580, 1951
Bsp1407I	TGTACA	1 cut(s)	1219
Bsp19I	CCATGG	1 cut(s)	1035
Bsp68I	TCGCGA	1 cut(s)	83
BspACI	CCGC	7 cut(s)	636, 666, 1215, 1645, 1711, 1815, 1857
BspANI	GGCC	1 cut(s)	1620
BspCNI	CTCAG	4 cut(s)	897, 930, 1638, 1934
BspDI	ATCGAT	1 cut(s)	1704
BspFNI	CGCG	2 cut(s)	83, 1817
BspLI	GGNNCC	9 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1960, 1966
BspMAI	CTGCAG	1 cut(s)	634
BspPI	GGATC	5 cut(s)	34, 422, 812, 1348, 1401
BspT104I	TTCGAA	2 cut(s)	1002, 1530
BspT107I	GGYRCC	4 cut(s)	60, 1211, 1946, 1958
BspTI	CTTAAG	1 cut(s)	1145
BspTNI	GGTCTC	3 cut(s)	422, 1718, 1805
BsrFI	RCCGGY	1 cut(s)	581
BsrGI	TGTACA	1 cut(s)	1219
BssAI	RCCGGY	1 cut(s)	581
BssNI	GRCGYC	1 cut(s)	1281
BssSI	CACGAG	1 cut(s)	10
BssT1I	CCWWGG	9 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1462, 1499
Bst2BI	CACGAG	1 cut(s)	10
Bst2UI	CCWGG	6 cut(s)	194, 269, 1120, 1373, 1391, 1631
Bst4CI	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
Bst6I	CTCTTC	3 cut(s)	444, 999, 1659
BstACI	GRCGYC	1 cut(s)	1281
BstAFI	CTTAAG	1 cut(s)	1145
BstAUI	TGTACA	1 cut(s)	1219
BstBAI	YACGTR	1 cut(s)	172
BstBI	TTCGAA	2 cut(s)	1002, 1530
BstC8I	GCNNGC	7 cut(s)	7, 42, 903, 926, 1069, 1711, 1773
BstDEI	CTNAG	6 cut(s)	261, 474, 884, 917, 1625, 1921
BstDSI	CCRYGG	1 cut(s)	1035
BstEII	GGTNACC	1 cut(s)	197
BstF5I	GGATG	6 cut(s)	427, 526, 583, 678, 1543, 1849
BstFNI	CGCG	2 cut(s)	83, 1817
BstH2I	RGCGCY	2 cut(s)	462, 1084
BstHHI	GCGC	2 cut(s)	461, 1083
BstMAI	GTCTC	9 cut(s)	235, 304, 422, 762, 1414, 1445, 1628, 1718, 1805
BstMCI	CGRYCG	1 cut(s)	48
BstMWI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1626
BstNI	CCWGG	6 cut(s)	194, 269, 1120, 1373, 1391, 1631
BstPAI	GACNNNNGTC	2 cut(s)	1406, 1730
BstPI	GGTNACC	1 cut(s)	197
BstSCI	CCNGG	8 cut(s)	192, 267, 1118, 1371, 1389, 1629, 1950, 1951
BstSFI	CTRYAG	1 cut(s)	630
BstSLI	GKGCMC	2 cut(s)	1580, 1951
BstUI	CGCG	2 cut(s)	83, 1817
BstV1I	GCAGC	3 cut(s)	244, 284, 644
BstV2I	GAAGAC	4 cut(s)	1414, 1575, 1686, 1875
BstX2I	RGATCY	1 cut(s)	26
BstYI	RGATCY	1 cut(s)	26
Bsu15I	ATCGAT	1 cut(s)	1704
BsuI	GTATCC	1 cut(s)	1559
BsuRI	GGCC	1 cut(s)	1620
BsuTUI	ATCGAT	1 cut(s)	1704
BtgI	CCRYGG	1 cut(s)	1035
BtgZI	GCGATG	2 cut(s)	64, 625
BtsCI	GGATG	6 cut(s)	427, 526, 583, 678, 1543, 1849
BtsIMutI	CAGTG	1 cut(s)	241
BtuMI	TCGCGA	1 cut(s)	83
Cac8I	GCNNGC	7 cut(s)	7, 42, 903, 926, 1069, 1711, 1773
CfoI	GCGC	2 cut(s)	461, 1083
Cfr10I	RCCGGY	1 cut(s)	581
Cfr13I	GGNCC	3 cut(s)	1040, 1132, 1618
Cfr9I	CCCGGG	1 cut(s)	1951
ClaI	ATCGAT	1 cut(s)	1704
CseI	GACGC	1 cut(s)	1073
Csp6I	GTAC	3 cut(s)	814, 1116, 1220
CviAII	CATG	8 cut(s)	216, 355, 463, 808, 1036, 1648, 1741, 1991
CviQI	GTAC	3 cut(s)	814, 1116, 1220
DdeI	CTNAG	6 cut(s)	261, 474, 884, 917, 1625, 1921
DriI	GACNNNNNGTC	1 cut(s)	1984
Eam1104I	CTCTTC	3 cut(s)	444, 999, 1659
Eam1105I	GACNNNNNGTC	1 cut(s)	1984
EarI	CTCTTC	3 cut(s)	444, 999, 1659
Ecl136II	GAGCTC	1 cut(s)	20
Eco130I	CCWWGG	9 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1462, 1499
Eco24I	GRGCYC	2 cut(s)	22, 874
Eco31I	GGTCTC	3 cut(s)	422, 1718, 1805
Eco47I	GGWCC	2 cut(s)	1040, 1132
Eco53kI	GAGCTC	1 cut(s)	20
Eco72I	CACGTG	1 cut(s)	172
Eco88I	CYCGRG	4 cut(s)	21, 1721, 1832, 1951
Eco91I	GGTNACC	1 cut(s)	197
EcoICRI	GAGCTC	1 cut(s)	20
EcoO109I	RGGNCCY	1 cut(s)	1040
EcoO65I	GGTNACC	1 cut(s)	197
EcoRI	GAATTC	1 cut(s)	1485
EcoRII	CCWGG	6 cut(s)	192, 267, 1118, 1371, 1389, 1629
EcoT14I	CCWWGG	9 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1462, 1499
EcoT38I	GRGCYC	2 cut(s)	22, 874
ErhI	CCWWGG	9 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1462, 1499
Esp3I	CGTCTC	2 cut(s)	1414, 1628
FaeI	CATG	8 cut(s)	219, 358, 466, 811, 1039, 1651, 1744, 1994
FalI	AAGNNNNNCTT	8 cut(s)	486, 518, 1139, 1171, 1254, 1286, 1755, 1787
FaqI	GGGAC	1 cut(s)	1053
FatI	CATG	8 cut(s)	215, 354, 462, 807, 1035, 1647, 1740, 1990
FauI	CCCGC	1 cut(s)	1718
FauNDI	CATATG	1 cut(s)	646
FblI	GTMKAC	2 cut(s)	1734, 1929
Fnu4HI	GCNGC	5 cut(s)	258, 273, 633, 636, 1215
FokI	GGATG	6 cut(s)	434, 533, 590, 665, 1550, 1856
FriOI	GRGCYC	2 cut(s)	22, 874
Fsp4HI	GCNGC	5 cut(s)	258, 273, 633, 636, 1215
FspBI	CTAG	2 cut(s)	609, 996
GlaI	GCGC	2 cut(s)	460, 1082
GluI	GCNGC	5 cut(s)	258, 273, 633, 636, 1215
GsaI	CCCAGC	1 cut(s)	597
GsuI	CTGGAG	1 cut(s)	1476
HaeII	RGCGCY	2 cut(s)	462, 1084
HaeIII	GGCC	1 cut(s)	1620
HapII	CCGG	4 cut(s)	582, 1482, 1952, 1962
HgaI	GACGC	1 cut(s)	1073
HhaI	GCGC	2 cut(s)	461, 1083
Hin1I	GRCGYC	1 cut(s)	1281
Hin1II	CATG	8 cut(s)	219, 358, 466, 811, 1039, 1651, 1744, 1994
Hin6I	GCGC	2 cut(s)	459, 1081
HinP1I	GCGC	2 cut(s)	459, 1081
HincII	GTYRAC	1 cut(s)	382
HindII	GTYRAC	1 cut(s)	382
HindIII	AAGCTT	4 cut(s)	359, 1331, 1769, 1781
HinfI	GANTC	7 cut(s)	950, 999, 1123, 1438, 1701, 1787, 1985
HpaI	GTTAAC	1 cut(s)	382
HpaII	CCGG	4 cut(s)	582, 1482, 1952, 1962
Hpy166II	GTNNAC	7 cut(s)	157, 280, 382, 970, 1186, 1735, 1930
Hpy188I	TCNGA	8 cut(s)	232, 320, 348, 979, 1028, 1591, 1792, 1972
Hpy188III	TCNNGA	7 cut(s)	82, 307, 886, 1234, 1552, 1723, 1941
Hpy8I	GTNNAC	7 cut(s)	157, 280, 382, 970, 1186, 1735, 1930
Hpy99I	CGWCG	4 cut(s)	38, 530, 1067, 1286
HpyAV	CCTTC	8 cut(s)	222, 475, 655, 1045, 1681, 1687, 1792, 1858
HpyCH4III	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
HpyCH4IV	ACGT	3 cut(s)	171, 1281, 1557
HpyCH4V	TGCA	5 cut(s)	71, 542, 632, 1455, 1908
HpyF10VI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1626
HpyF3I	CTNAG	6 cut(s)	261, 474, 884, 917, 1625, 1921
HpySE526I	ACGT	3 cut(s)	171, 1281, 1557
Hsp92I	GRCGYC	1 cut(s)	1281
Hsp92II	CATG	8 cut(s)	219, 358, 466, 811, 1039, 1651, 1744, 1994
HspAI	GCGC	2 cut(s)	459, 1081
KspAI	GTTAAC	1 cut(s)	382
LmnI	GCTCC	3 cut(s)	1168, 1501, 1964
Lsp1109I	GCAGC	3 cut(s)	244, 284, 644
LweI	GCATC	6 cut(s)	511, 544, 568, 1239, 1528, 1696
MaeI	CTAG	2 cut(s)	609, 996
MaeII	ACGT	3 cut(s)	171, 1281, 1557
MaeIII	GTNAC	6 cut(s)	49, 167, 197, 529, 1522, 1986
MfeI	CAATTG	1 cut(s)	729
MflI	RGATCY	1 cut(s)	26
MhlI	GDGCHC	5 cut(s)	22, 874, 916, 1580, 1951
MluCI	AATT	6 cut(s)	72, 120, 177, 729, 1469, 1485
MlyI	GAGTC	3 cut(s)	1447, 1796, 1994
MmeI	TCCRAC	3 cut(s)	1159, 1294, 1821
MseI	TTAA	5 cut(s)	381, 831, 1146, 1653, 2002
MslI	CAYNNNNRTG	1 cut(s)	58
MspA1I	CMGCKG	2 cut(s)	275, 1217
MspCI	CTTAAG	1 cut(s)	1145
MspI	CCGG	4 cut(s)	582, 1482, 1952, 1962
MspR9I	CCNGG	8 cut(s)	194, 269, 1120, 1373, 1391, 1631, 1952, 1953
MunI	CAATTG	1 cut(s)	729
Mva1269I	GAATGC	1 cut(s)	497
MvaI	CCWGG	6 cut(s)	194, 269, 1120, 1373, 1391, 1631
MvnI	CGCG	2 cut(s)	83, 1817
MwoI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1626
NciI	CCSGG	2 cut(s)	1952, 1953
NcoI	CCATGG	1 cut(s)	1035
NdeI	CATATG	1 cut(s)	646
NlaIII	CATG	8 cut(s)	219, 358, 466, 811, 1039, 1651, 1744, 1994
NlaIV	GGNNCC	9 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1960, 1966
NmeAIII	GCCGAG	3 cut(s)	922, 1699, 1885
NmuCI	GTSAC	4 cut(s)	49, 167, 1522, 1986
NruI	TCGCGA	1 cut(s)	83
NspV	TTCGAA	2 cut(s)	1002, 1530
PaeR7I	CTCGAG	3 cut(s)	21, 1721, 1832
PctI	GAATGC	1 cut(s)	497
PfeI	GAWTC	4 cut(s)	950, 999, 1123, 1701
PkrI	GCNGC	5 cut(s)	259, 274, 634, 637, 1216
Ple19I	CGATCG	1 cut(s)	48
PleI	GAGTC	3 cut(s)	1446, 1795, 1993
PmaCI	CACGTG	1 cut(s)	172
PmlI	CACGTG	1 cut(s)	172
PpsI	GAGTC	3 cut(s)	1446, 1795, 1993
Ppu21I	YACGTR	1 cut(s)	172
PpuMI	RGGWCCY	1 cut(s)	1040
PshAI	GACNNNNGTC	2 cut(s)	1406, 1730
Psp124BI	GAGCTC	1 cut(s)	22
Psp5II	RGGWCCY	1 cut(s)	1040
Psp6I	CCWGG	6 cut(s)	192, 267, 1118, 1371, 1389, 1629
PspCI	CACGTG	1 cut(s)	172
PspEI	GGTNACC	1 cut(s)	197
PspFI	CCCAGC	1 cut(s)	593
PspGI	CCWGG	6 cut(s)	192, 267, 1118, 1371, 1389, 1629
PspN4I	GGNNCC	9 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1960, 1966
PspPI	GGNCC	3 cut(s)	1040, 1132, 1618
PspPPI	RGGWCCY	1 cut(s)	1040
PspXI	VCTCGAGB	2 cut(s)	21, 1832
PstI	CTGCAG	1 cut(s)	634
PsuI	RGATCY	1 cut(s)	26
PvuI	CGATCG	1 cut(s)	48
PvuII	CAGCTG	1 cut(s)	275
RruI	TCGCGA	1 cut(s)	83
RsaI	GTAC	3 cut(s)	815, 1117, 1221
RsaNI	GTAC	3 cut(s)	814, 1116, 1220
RseI	CAYNNNNRTG	1 cut(s)	58
SacI	GAGCTC	1 cut(s)	22
SaqAI	TTAA	5 cut(s)	381, 831, 1146, 1653, 2002
SatI	GCNGC	5 cut(s)	258, 273, 633, 636, 1215
Sau96I	GGNCC	3 cut(s)	1040, 1132, 1618
ScaI	AGTACT	1 cut(s)	815
SchI	GAGTC	3 cut(s)	1447, 1796, 1994
ScrFI	CCNGG	8 cut(s)	194, 269, 1120, 1373, 1391, 1631, 1952, 1953
SduI	GDGCHC	5 cut(s)	22, 874, 916, 1580, 1951
SfaNI	GCATC	6 cut(s)	511, 544, 568, 1239, 1528, 1696
SfcI	CTRYAG	1 cut(s)	630
Sfr274I	CTCGAG	3 cut(s)	21, 1721, 1832
SfuI	TTCGAA	2 cut(s)	1002, 1530
SinI	GGWCC	2 cut(s)	1040, 1132
SlaI	CTCGAG	3 cut(s)	21, 1721, 1832
SmaI	CCCGGG	1 cut(s)	1953
SmiMI	CAYNNNNRTG	1 cut(s)	58
SmlI	CTYRAG	7 cut(s)	21, 1145, 1234, 1721, 1784, 1832, 1886
SmoI	CTYRAG	7 cut(s)	21, 1145, 1234, 1721, 1784, 1832, 1886
Sse9I	AATT	6 cut(s)	72, 120, 177, 729, 1469, 1485
SsiI	CCGC	7 cut(s)	636, 666, 1215, 1645, 1711, 1815, 1857
SspMI	CTAG	2 cut(s)	609, 996
SstI	GAGCTC	1 cut(s)	22
StyD4I	CCNGG	8 cut(s)	192, 267, 1118, 1371, 1389, 1629, 1950, 1951
StyI	CCWWGG	9 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1462, 1499
TaaI	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
TaiI	ACGT	3 cut(s)	174, 1284, 1560
TaqI	TCGA	9 cut(s)	22, 747, 1002, 1425, 1518, 1530, 1704, 1722, 1833
TasI	AATT	6 cut(s)	72, 120, 177, 729, 1469, 1485
TatI	WGTACW	2 cut(s)	813, 1219
TauI	GCSGC	2 cut(s)	638, 1217
TfiI	GAWTC	4 cut(s)	950, 999, 1123, 1701
Tru1I	TTAA	5 cut(s)	381, 831, 1146, 1653, 2002
Tru9I	TTAA	5 cut(s)	381, 831, 1146, 1653, 2002
TscAI	CASTG	1 cut(s)	241
TseFI	GTSAC	4 cut(s)	49, 167, 1522, 1986
TseI	GCWGC	3 cut(s)	257, 272, 632
Tsp45I	GTSAC	4 cut(s)	49, 167, 1522, 1986
TspDTI	ATGAA	6 cut(s)	204, 371, 494, 636, 808, 1757
TspGWI	ACGGA	2 cut(s)	858, 926
TspMI	CCCGGG	1 cut(s)	1951
TspRI	CASTG	1 cut(s)	241
Vha464I	CTTAAG	1 cut(s)	1145
VpaK11BI	GGWCC	2 cut(s)	1040, 1132
XapI	RAATTY	2 cut(s)	1469, 1485
XhoI	CTCGAG	3 cut(s)	21, 1721, 1832
XmaI	CCCGGG	1 cut(s)	1951
XmiI	GTMKAC	2 cut(s)	1734, 1929
XspI	CTAG	2 cut(s)	609, 996
ZraI	GACGTC	1 cut(s)	1282
ZrmI	AGTACT	1 cut(s)	815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.