Rh7CG080100
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003307
Physical Location & Seq
Forward (+)
5815952 .. 5819772
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG080100.1

Sequence Viewer

Length: 756 bp
ATGTTTGTATTCAATGCAGATATTCTTCTCTTGGATGTTGCACCTCTTACCCTTGGTATTGAGACTGTTGGTGGAGTGATGACCAAGTTGATCCCCAGAAACACTGTGATCCCAACCAAGAAGTCCCAAGTTTTCACCACTTACCAGGATCAGCAGACTACTGTCTCCATTCAGGTCTTTGAGGGTGAAAGGAGTCTCACAAAGGATTGTAGAAACCTTGGAAAGTTCGACCTTACAGGAATTCCTCCAGCTCCAAGGGGTACTCCTCAGATCGAAGTCACCTTCGAAGTGGATGCCAACGGTATCCTGAATGTCAAGGCAGAAGACAAGGGCACTGGTAAATCTGAGAAGATCACCATCACCAACGACAAGGGTCGTCTGAGCCAGGAGGAGATTGAGCGCATGGTTAAGGAGGCCGAGGAGTTTGCTGAGGAAGACAAGAAGGTCAAGGAGAGAATTGACGCCCGTAACAGCCTCGAAACCTACACTTACAACATGAAGAACCAAGTCAATGACAAGGACAAGCTTGCTGACAAGCTCGAGTCTGATGAGAAGGAGAAGATTGAGACCGCAGTGAAGGAAGCCCTTGAATGGCTGGACGACAACCAGACCGCTGAGAAGGAAGACTATGAGGAGAAGCTCAAGGAGGTCGAAGCCGTCTGCAACCCCATCATCACAGCTGTCTACCAGAGGTCAGGTGGCGCCCCAGGCGGTGAATCAGCATCCGAGGATGATGATTCTCATGACGAGCTCTAA

Protein Analysis

251

Amino Acids

28.07

Weight (kDa)

4.56

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 6 - 230 1.3e-75 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 684
AciI CCGC 3 cut(s) 570, 612, 711
AclWI GGATC 3 cut(s) 85, 103, 156
AcsI RAATTY 1 cut(s) 240
AcyI GRCGYC 2 cut(s) 462, 702
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 1 cut(s) 591
AgsI TTSAA 2 cut(s) 13, 590
AjnI CCWGG 3 cut(s) 144, 384, 706
AluBI AGCT 6 cut(s) 251, 526, 538, 640, 680, 751
AluI AGCT 6 cut(s) 251, 526, 538, 640, 680, 751
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 4 cut(s) 56, 169, 200, 560
AlwI GGATC 3 cut(s) 85, 103, 156
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 1 cut(s) 414
ApoI RAATTY 1 cut(s) 240
AspLEI GCGC 2 cut(s) 402, 704
AsuHPI GGTGA 6 cut(s) 127, 197, 271, 346, 352, 725
AsuII TTCGAA 1 cut(s) 285
AvaI CYCGRG 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 753
BbsI GAAGAC 3 cut(s) 330, 441, 630
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 429
BccI CCATC 2 cut(s) 365, 677
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 4 cut(s) 275, 309, 407, 441
BciT130I CCWGG 3 cut(s) 146, 386, 708
BciVI GTATCC 1 cut(s) 314
BcoDI GTCTC 4 cut(s) 56, 169, 200, 560
BfoI RGCGCY 1 cut(s) 705
BfuI GTATCC 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 146, 386, 708
BmeT110I CYCGRG 1 cut(s) 539
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 3 cut(s) 146, 386, 708
BmsI GCATC 2 cut(s) 283, 731
BoxI GACNNNNGTC 2 cut(s) 161, 372
BpiI GAAGAC 3 cut(s) 330, 441, 630
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 626
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 462, 702
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 6 cut(s) 52, 217, 254, 417, 706, 726
Bsc4I CCNNNNNNNGG 1 cut(s) 591
Bse1I ACTGG 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 3 cut(s) 146, 386, 708
BseDI CCNNGG 6 cut(s) 52, 217, 254, 417, 706, 726
BseGI GGATG 4 cut(s) 40, 298, 722, 736
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 1 cut(s) 591
BseMII CTCAG 5 cut(s) 281, 336, 371, 420, 606
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 4 cut(s) 255, 404, 434, 647
BseSI GKGCMC 1 cut(s) 335
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 591
BsmAI GTCTC 4 cut(s) 56, 169, 200, 560
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 560
BsoBI CYCGRG 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 285
Bsp1286I GDGCHC 2 cut(s) 335, 753
Bsp143I GATC 5 cut(s) 90, 108, 148, 270, 351
BspACI CCGC 3 cut(s) 570, 612, 711
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 5 cut(s) 280, 337, 372, 421, 607
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 1 cut(s) 703
BspPI GGATC 3 cut(s) 85, 103, 156
BspT104I TTCGAA 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 560
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 6 cut(s) 52, 217, 254, 417, 706, 726
BssMI GATC 5 cut(s) 90, 108, 148, 270, 351
BssNI GRCGYC 2 cut(s) 462, 702
BssT1I CCWWGG 3 cut(s) 52, 217, 254
Bst2UI CCWGG 3 cut(s) 146, 386, 708
Bst4CI ACNGT 4 cut(s) 67, 106, 163, 302
BstACI GRCGYC 2 cut(s) 462, 702
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 5 cut(s) 267, 345, 380, 429, 615
BstF5I GGATG 4 cut(s) 40, 298, 722, 736
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 402, 704
BstKTI GATC 5 cut(s) 93, 111, 151, 273, 354
BstMAI GTCTC 4 cut(s) 56, 169, 200, 560
BstMBI GATC 5 cut(s) 90, 108, 148, 270, 351
BstMWI GCNNNNNNNGC 1 cut(s) 708
BstNI CCWGG 3 cut(s) 146, 386, 708
BstPAI GACNNNNGTC 2 cut(s) 161, 372
BstSCI CCNGG 3 cut(s) 144, 384, 706
BstSLI GKGCMC 1 cut(s) 335
BstV2I GAAGAC 3 cut(s) 330, 441, 630
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 1 cut(s) 416
BtsCI GGATG 4 cut(s) 40, 298, 722, 736
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 3 cut(s) 102, 333, 579
Cac8I GCNNGC 1 cut(s) 528
CciI TCATGA 1 cut(s) 742
CfoI GCGC 2 cut(s) 402, 704
CseI GACGC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 261
CviAII CATG 3 cut(s) 403, 496, 743
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 5 cut(s) 267, 345, 380, 429, 615
DinI GGCGCC 1 cut(s) 703
DpnI GATC 5 cut(s) 92, 110, 150, 272, 353
DpnII GATC 5 cut(s) 90, 108, 148, 270, 351
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 751
Eco130I CCWWGG 3 cut(s) 52, 217, 254
Eco24I GRGCYC 1 cut(s) 753
Eco31I GGTCTC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 751
Eco88I CYCGRG 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 240
EcoRII CCWGG 3 cut(s) 144, 384, 706
EcoT14I CCWWGG 3 cut(s) 52, 217, 254
EcoT38I GRGCYC 1 cut(s) 753
EgeI GGCGCC 1 cut(s) 703
EheI GGCGCC 1 cut(s) 703
ErhI CCWWGG 3 cut(s) 52, 217, 254
FaeI CATG 3 cut(s) 406, 499, 746
FaiI YATR 4 cut(s) 404, 497, 630, 744
FaqI GGGAC 1 cut(s) 109
FatI CATG 3 cut(s) 402, 495, 742
FblI GTMKAC 1 cut(s) 684
FokI GGATG 4 cut(s) 47, 305, 709, 743
FriOI GRGCYC 1 cut(s) 753
GlaI GCGC 2 cut(s) 401, 703
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 1 cut(s) 416
HgaI GACGC 1 cut(s) 470
HhaI GCGC 2 cut(s) 402, 704
Hin1I GRCGYC 2 cut(s) 462, 702
Hin1II CATG 3 cut(s) 406, 499, 746
Hin6I GCGC 2 cut(s) 400, 702
HinP1I GCGC 2 cut(s) 400, 702
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 4 cut(s) 193, 542, 716, 737
HphI GGTGA 6 cut(s) 127, 197, 271, 346, 352, 725
Hpy166II GTNNAC 1 cut(s) 685
Hpy188I TCNGA 5 cut(s) 270, 346, 381, 547, 727
Hpy188III TCNNGA 2 cut(s) 307, 743
Hpy8I GTNNAC 1 cut(s) 685
HpyAV CCTTC 5 cut(s) 292, 436, 547, 571, 613
HpyCH4III ACNGT 4 cut(s) 67, 106, 163, 302
HpyCH4V TGCA 3 cut(s) 17, 41, 663
HpyF10VI GCNNNNNNNGC 1 cut(s) 708
HpyF3I CTNAG 5 cut(s) 267, 345, 380, 429, 615
Hsp92I GRCGYC 2 cut(s) 462, 702
Hsp92II CATG 3 cut(s) 406, 499, 746
HspAI GCGC 2 cut(s) 400, 702
KasI GGCGCC 1 cut(s) 701
Kzo9I GATC 5 cut(s) 90, 108, 148, 270, 351
LmnI GCTCC 1 cut(s) 256
LweI GCATC 2 cut(s) 283, 731
MaeIII GTNAC 2 cut(s) 277, 467
MalI GATC 5 cut(s) 92, 110, 150, 272, 353
MboI GATC 5 cut(s) 90, 108, 148, 270, 351
MboII GAAGA 7 cut(s) 17, 335, 361, 446, 511, 571, 635
MhlI GDGCHC 2 cut(s) 335, 753
MluCI AATT 2 cut(s) 240, 456
Mly113I GGCGCC 1 cut(s) 702
MlyI GAGTC 2 cut(s) 202, 551
MseI TTAA 1 cut(s) 408
MspA1I CMGCKG 2 cut(s) 614, 680
MspR9I CCNGG 3 cut(s) 146, 386, 708
MvaI CCWGG 3 cut(s) 146, 386, 708
MwoI GCNNNNNNNGC 1 cut(s) 708
NarI GGCGCC 1 cut(s) 702
NdeII GATC 5 cut(s) 90, 108, 148, 270, 351
NlaIII CATG 3 cut(s) 406, 499, 746
NlaIV GGNNCC 1 cut(s) 703
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 277
NspV TTCGAA 1 cut(s) 285
PaeR7I CTCGAG 1 cut(s) 539
PagI TCATGA 1 cut(s) 742
PfeI GAWTC 2 cut(s) 716, 737
PleI GAGTC 2 cut(s) 201, 550
PluTI GGCGCC 1 cut(s) 705
PpsI GAGTC 2 cut(s) 201, 550
PshAI GACNNNNGTC 2 cut(s) 161, 372
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 3 cut(s) 144, 384, 706
PspGI CCWGG 3 cut(s) 144, 384, 706
PspN4I GGNNCC 1 cut(s) 703
PspXI VCTCGAGB 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SacI GAGCTC 1 cut(s) 753
SaqAI TTAA 1 cut(s) 408
Sau3AI GATC 5 cut(s) 90, 108, 148, 270, 351
SchI GAGTC 2 cut(s) 202, 551
ScrFI CCNGG 3 cut(s) 146, 386, 708
SduI GDGCHC 2 cut(s) 335, 753
SfaNI GCATC 2 cut(s) 283, 731
SfoI GGCGCC 1 cut(s) 703
Sfr274I CTCGAG 1 cut(s) 539
SfuI TTCGAA 1 cut(s) 285
SlaI CTCGAG 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 539, 641
SmoI CTYRAG 2 cut(s) 539, 641
Sse9I AATT 2 cut(s) 240, 456
SsiI CCGC 3 cut(s) 570, 612, 711
SspDI GGCGCC 1 cut(s) 701
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 3 cut(s) 144, 384, 706
StyI CCWWGG 3 cut(s) 52, 217, 254
TaaI ACNGT 4 cut(s) 67, 106, 163, 302
TaqI TCGA 6 cut(s) 228, 273, 285, 477, 540, 651
TasI AATT 2 cut(s) 240, 456
TfiI GAWTC 2 cut(s) 716, 737
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 3 cut(s) 109, 340, 579
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 1 cut(s) 512
TspRI CASTG 3 cut(s) 109, 340, 579
XapI RAATTY 1 cut(s) 240
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XhoI CTCGAG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.