Rw5G015350
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003307
Physical Location & Seq
Forward (+)
19090719 .. 19092370
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015350.1

Sequence Viewer

Length: 861 bp
ATGGCTCTTCTAGATGAGATTGCAATACGGTTGAGAGACTCTGATGAGCAAGTGGCAGCTGTTTTAACAGATGATGGTTTTGATGAACAATATGAACATCATTTGGAACTTTTTAAGGATGAACTAGAGATGTTGGAAGATGAAATAGAGTTAGAAGAACTCGAAAAAATGGTGGAACTTGAACAAGAATTGAAAGAACTTGAACTGTTGGAGGTTGCAGGAGAAATGGAACAACTTGAAATGTTGGCTATTGAAGGTTCATATCCCTACTTAAACAATGGTGTGAACCCTGAGGCATTTGCTTATGATAAGGCTGGTGATGGAAACAAAGATATCATTCTCTGGGATGTTGCACCTCTCACCCTTGGTATCGAAACTGCTGGTGGTATTATGACTAAATTGATTCCCAGAAACACTGTGATCCCAACCAAGAAATCCCAGGTTTTCACCACTTATCAGGATCAGCAGAGTACAGTCTACATTCAGGTCTATGGGGGTGAAGGGAGTCTAACAAGATATTGCAGGATCCTTGGAAAATTTGAACTTACTGGCATTCCCCCAGCCCCAAGGTTTGTTTCTGCTCCAAAGTGTATGAGGAAGGTGAAGGAGAGGATTGATGCCCATAATAGCCTGAAAAGTTTTATCTACAACTTGAAGAACCAGATCAACGAAATGGACAAGCTTGAGTATGATGAGAAGGATAAGCTTGAGACCGCTGTGAAGAAGGCCCTGGAATGGATGAAGGACAACCAGACCGCTGAGAAGGAGGACTATGAGGAGAAGCTCAAGGAGGTTGAAGCAATCTGCAACGCTATCATCACAACTGTTTACCAGAGGTCAGGTGATACTCCTGTGGATTAA

Protein Analysis

286

Amino Acids

32.7

Weight (kDa)

4.48

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 108 - 191 3.5e-28 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AciI CCGC 2 cut(s) 714, 756
AclWI GGATC 4 cut(s) 415, 468, 520, 533
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 735
AgsI TTSAA 8 cut(s) 182, 193, 203, 239, 254, 542, 655, 797
AjnI CCWGG 2 cut(s) 438, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AluBI AGCT 4 cut(s) 59, 682, 706, 784
AluI AGCT 4 cut(s) 59, 682, 706, 784
Alw26I GTCTC 2 cut(s) 30, 704
AlwI GGATC 4 cut(s) 415, 468, 520, 533
AoxI GGCC 1 cut(s) 726
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 536
AspS9I GGNCC 1 cut(s) 727
AsuHPI GGTGA 6 cut(s) 329, 352, 439, 509, 613, 854
AxyI CCTNAGG 1 cut(s) 291
BaeI ACNNNNGTAYC 1 cut(s) 837
BamHI GGATCC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 68, 314
BciT130I CCWGG 2 cut(s) 440, 731
BcoDI GTCTC 2 cut(s) 30, 704
BfaI CTAG 2 cut(s) 11, 125
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 440, 731
BmgT120I GGNCC 1 cut(s) 727
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 2 cut(s) 440, 731
BmsI GCATC 1 cut(s) 607
BpuEI CTTGAG 3 cut(s) 704, 728, 770
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 5 cut(s) 364, 438, 529, 566, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 735
Bse1I ACTGG 1 cut(s) 553
Bse21I CCTNAGG 1 cut(s) 291
BseBI CCWGG 2 cut(s) 440, 731
BseDI CCNNGG 5 cut(s) 364, 438, 529, 566, 729
BseGI GGATG 3 cut(s) 124, 352, 744
BseLI CCNNNNNNNGG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 282, 750
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 559
BshFI GGCC 1 cut(s) 728
BslI CCNNNNNNNGG 1 cut(s) 735
BsmAI GTCTC 2 cut(s) 30, 704
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 1 cut(s) 728
Bso31I GGTCTC 1 cut(s) 704
Bsp143I GATC 4 cut(s) 420, 460, 525, 663
BspACI CCGC 2 cut(s) 714, 756
BspANI GGCC 1 cut(s) 728
BspCNI CTCAG 2 cut(s) 283, 751
BspLI GGNNCC 1 cut(s) 527
BspPI GGATC 4 cut(s) 415, 468, 520, 533
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 704
BsrI ACTGG 1 cut(s) 553
BssECI CCNNGG 5 cut(s) 364, 438, 529, 566, 729
BssMI GATC 4 cut(s) 420, 460, 525, 663
BssT1I CCWWGG 3 cut(s) 364, 529, 566
Bst2UI CCWGG 2 cut(s) 440, 731
Bst4CI ACNGT 5 cut(s) 30, 207, 418, 475, 826
Bst6I CTCTTC 1 cut(s) 12
BstDEI CTNAG 2 cut(s) 291, 759
BstF5I GGATG 3 cut(s) 124, 352, 744
BstKTI GATC 4 cut(s) 423, 463, 528, 666
BstMAI GTCTC 2 cut(s) 30, 704
BstMBI GATC 4 cut(s) 420, 460, 525, 663
BstNI CCWGG 2 cut(s) 440, 731
BstSCI CCNGG 2 cut(s) 438, 729
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 1 cut(s) 728
BtsCI GGATG 3 cut(s) 124, 352, 744
BtsIMutI CAGTG 1 cut(s) 414
Cfr13I GGNCC 1 cut(s) 727
Csp6I GTAC 1 cut(s) 471
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 2 cut(s) 291, 759
DpnI GATC 4 cut(s) 422, 462, 527, 665
DpnII GATC 4 cut(s) 420, 460, 525, 663
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 364, 529, 566
Eco31I GGTCTC 1 cut(s) 704
Eco32I GATATC 1 cut(s) 334
Eco81I CCTNAGG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 727
EcoRII CCWGG 2 cut(s) 438, 729
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 3 cut(s) 364, 529, 566
ErhI CCWWGG 3 cut(s) 364, 529, 566
FaiI YATR 9 cut(s) 93, 262, 306, 392, 492, 593, 624, 690, 774
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 3 cut(s) 131, 359, 751
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 2 cut(s) 11, 125
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 563
HaeIII GGCC 1 cut(s) 728
HindIII AAGCTT 2 cut(s) 680, 704
HinfI GANTC 3 cut(s) 38, 403, 505
HphI GGTGA 6 cut(s) 329, 352, 439, 509, 613, 854
Hpy166II GTNNAC 3 cut(s) 286, 478, 829
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 2 cut(s) 11, 458
Hpy8I GTNNAC 3 cut(s) 286, 478, 829
HpyAV CCTTC 8 cut(s) 248, 494, 592, 598, 691, 718, 736, 757
HpyCH4III ACNGT 5 cut(s) 30, 207, 418, 475, 826
HpyCH4V TGCA 5 cut(s) 23, 218, 353, 522, 807
HpyF3I CTNAG 2 cut(s) 291, 759
Kzo9I GATC 4 cut(s) 420, 460, 525, 663
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 586
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 11, 125
MalI GATC 4 cut(s) 422, 462, 527, 665
MboI GATC 4 cut(s) 420, 460, 525, 663
MboII GAAGA 4 cut(s) 149, 167, 667, 733
MflI RGATCY 1 cut(s) 525
MluCI AATT 3 cut(s) 188, 398, 536
MlyI GAGTC 2 cut(s) 32, 514
MmeI TCCRAC 2 cut(s) 114, 189
MnlI CCTC 9 cut(s) 205, 286, 366, 588, 603, 760, 769, 784, 828
MseI TTAA 4 cut(s) 65, 114, 272, 859
MspA1I CMGCKG 3 cut(s) 59, 716, 758
MspR9I CCNGG 2 cut(s) 440, 731
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 2 cut(s) 440, 731
NdeII GATC 4 cut(s) 420, 460, 525, 663
NlaIV GGNNCC 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 1 cut(s) 403
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 2 cut(s) 32, 513
PpsI GAGTC 2 cut(s) 32, 513
Psp6I CCWGG 2 cut(s) 438, 729
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 2 cut(s) 438, 729
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 1 cut(s) 727
PsuI RGATCY 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 4 cut(s) 65, 114, 272, 859
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 420, 460, 525, 663
Sau96I GGNCC 1 cut(s) 727
SchI GAGTC 2 cut(s) 32, 514
ScrFI CCNGG 2 cut(s) 440, 731
SfaNI GCATC 1 cut(s) 607
SmlI CTYRAG 3 cut(s) 683, 707, 785
SmoI CTYRAG 3 cut(s) 683, 707, 785
Sse9I AATT 3 cut(s) 188, 398, 536
SsiI CCGC 2 cut(s) 714, 756
SspMI CTAG 2 cut(s) 11, 125
StyD4I CCNGG 2 cut(s) 438, 729
StyI CCWWGG 3 cut(s) 364, 529, 566
TaaI ACNGT 5 cut(s) 30, 207, 418, 475, 826
TaqI TCGA 2 cut(s) 162, 372
TasI AATT 3 cut(s) 188, 398, 536
TatI WGTACW 1 cut(s) 470
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 4 cut(s) 65, 114, 272, 859
Tru9I TTAA 4 cut(s) 65, 114, 272, 859
TscAI CASTG 1 cut(s) 421
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 6 cut(s) 99, 108, 135, 156, 249, 755
TspRI CASTG 1 cut(s) 421
XapI RAATTY 1 cut(s) 536
XbaI TCTAGA 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 2 cut(s) 11, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.