Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003307

Physical Location & Seq

Genomic Coordinates Forward (+)

21236326 .. 21240511

Gene Structure 4186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1134100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGCTGGCTCTTGGAGAGCTCGCGGATCTCTGATCGTGCTCGCGATCGTCACATTCGGGTGCCTTTTTGCATTTTCCATTGCTAAAGAAGAAGCGACCAAGTTGGGAACTGTCATTGGAATTGATCTTGGGACCACTTATTCCTGTGTTGGTGTTTACAAGAATGGTCATGTGGAAATTATAGCCAATGACCAGGGTAACCGTATCACCCCATCATGGGTGGCATTCAGCGACAGTGAGAGACTGATCGGTGAGGCTGCTAAGAACCAGGCAGCTGTGAACCCTGAAAGGACCGTCTTTGATGTCAAGAGACTTATCGGAAGAAAGTTTGAAGACAAAGAGGTTCAGAAGGACATGAAGCTTGTTCCTTACAAGATTGTTAATAGAGATGGAAAACCTTATATCCAGGTGAGGATCAAGGATGGTGAAACCAAGGTTTTCAGCCCCGAAGAGGTCAGCGCCATGATTTTGACTAAGATGAAGGAAACGGCTGAAGCATTCCTTGGGAAGAAAATCAAGGATGCCGTCGTTACTGTCCCTGCATACTTCAATGATGCCCAAAGACAGGCAACCAAGGATGCCGGTGTGATTGCTGGGCTGAATGTTGCTAGGATTATCAATGAACCAACCGCAGCCGCCATCGCATATGGTTTGGACAAGAAGGGTGGCGAAAAGAACATCCTTGTGTTTGACCTTGGTGGTGGAACTTTCGATGTCAGTATTTTGACAATTGATAATGGTGTTTTTGAGGTTCTTTCCACAAATGGAGACACCCATTTGGGAGGTGAGGACTTTGACCAGAGGATCATGGAGTACTTCATCAAGTTGATTAAGAAGAAGCACGGAAAGGACATCAGCAAGGACAACAGAGCCCTTGGAAAACTCAGGAGGGAAGCCGAGCGTGCCAAGAGAGCACTCAGTAGCCAGCACCAGGTCCGTGTGGAGATTGAATCCCTTTTCGATGGAGTGGACTTCTCTGAACCCCTAACCAGAGCTAGATTCGAAGAGTTGAACAATGACTTGTTCAGAAAGACCATGGGACCTGTGAAGAAGGCTATGGATGATGCTGGCTTGGAGAAGCGACAGATCGACGAGATTGTGCTTGTTGGTGGAAGTACCAGGATTCCCAAGGTCCAGCAGCTTCTTAAGGACTACTTTGATGGCAAGGAGCCAAACAAGGGTGTGAACCCTGATGAGGCTGTTGCTTATGGTGCCGCTGTACAAGGAAGCATCTTGAGTGGAGAGGGTGGTGAAGAAACCAAAGATATCCTTCTCCTTGATGTCGCTCCTCTCACCCTCGGTATTGAAACTGTTGGAGGAGTTATGACCAAGCTTATCCCAAGAAACACCGTTATTCCAACCAAGAAATCCCAGGTTTTCACCACTTACCAGGATCAGCAGACAACTGTCTCCATTCAGGTCTTTGAGGGTGAAAGGAGTCTCACAAAAGATTGCAGGAACCTCGGTAAATTTGATCTCACCGGAATTCCTCCAGCTCCAAGGGGAACTCCTCAAATCGAAGTCACATTCGAAGTTGATGCTAACGGTATCCTTAACGTGAGGGCAGAAGACAAGGGCACGGGTAAAGCTGAAAAGATCACCATCACAAACGACAAGGGTCGTCTCAGCCAGGAGGAGATTGAGCGCATGGTTAAGGAGGCAGAGGAGTTTGCCGAGGAAGACAAGAAGGTGAAGGAGAGGATCGATGCTCGCAACAGCCTGGAGACCTACGTCTACAACATGAAGAACCAGATCAACGACAAAGACAAGCTTGCTGACAAGCTCGAGTCCGATGAGAAGGAAAAGATTGAAACCGCCACAAAGGAAGCCCTCGAATGGTTGGACGACAACCAGACTGCCGAGAAGGAAGACTACGATGAGAAGCTCAAGGAGGTTGAAGCCGTGTGCAACCCCATCATCTCAGCCGTATACCAGAGGTCAGGAGGTGCCCCAGGTGGTGCTGGAGCCTCAGAGGAGGACGATGAGTCGCATGACGAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0012505 GO:0031974 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

668

Amino Acids

73.61

Weight (kDa)

5.14

Isoelectric Point (pI)

28.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	38 - 645	8.8e-265	Hsp70 protein
MreB_Mbl	PF06723	153 - 405	1.3e-15	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28540 AT5G42020 AT5G42020 AT5G42020
fragaria_vesca	FvH4_3g14480 FvH4_5g11500 FvH4_5g11500
malus_domestica	MD06G1113000.v1.1 MD14G1134100.v1.1
prunus_persica	Prupe.5G125900_v2.0.a1 Prupe.5G125900_v2.0.a1 Prupe.5G125900_v2.0.a1
pyrus_communis	pycom06g10560 pycom06g10570
rosa_chinensis	RchiOBHm_Chr5g0023851 RchiOBHm_Chr7g0186301
rosa_laevigata	RLG00000004851 RLG00000032740
rosa_multiflora	Rmu_co8308791.1_g000001 Rmu_sc0001372.1_g000006 Rmu_sc0018092.1_g000001 Rmu_ssc0000027.1_g000008
rosa_roxburghii	Rroxscaffold_1G00055390 Rroxscaffold_3G00268240
rosa_rugosa	Rorug05G0079800 Rorug06G0473100
rosa_samantha	Rh5AG169800 Rh5BG167100 Rh5CG182700 Rh5DG169200 Rh7AG067900 Rh7BG083700 Rh7CG080100 Rh7DG080700
rosa_wichuraiana	Rw5G015350 Rw7G006710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	60, 1211, 1946
AccI	GTMKAC	2 cut(s)	1734, 1929
AccII	CGCG	2 cut(s)	24, 44
AciI	CCGC	5 cut(s)	24, 630, 636, 1215, 1815
AclWI	GGATC	5 cut(s)	34, 422, 812, 1401, 1709
AcsI	RAATTY	2 cut(s)	1469, 1485
AcuI	CTGAAG	1 cut(s)	513
AfaI	GTAC	3 cut(s)	815, 1117, 1221
AfiI	CCNNNNNNNGG	8 cut(s)	216, 217, 451, 565, 931, 1178, 1195, 1836
AflII	CTTAAG	1 cut(s)	1145
AgsI	TTSAA	7 cut(s)	332, 550, 950, 1012, 1307, 1811, 1898
AhdI	GACNNNNNGTC	1 cut(s)	1984
AjuI	GAANNNNNNNTTGG	2 cut(s)	486, 518
Alw21I	GWGCWC	3 cut(s)	22, 42, 916
Alw26I	GTCTC	7 cut(s)	235, 304, 762, 1414, 1445, 1628, 1718
AlwI	GGATC	5 cut(s)	34, 422, 812, 1401, 1709
Ama87I	CYCGRG	1 cut(s)	1784
ApeKI	GCWGC	4 cut(s)	257, 272, 632, 1138
ApoI	RAATTY	2 cut(s)	1469, 1485
AspLEI	GCGC	2 cut(s)	461, 1647
AspS9I	GGNCC	5 cut(s)	132, 291, 934, 1040, 1132
AsuII	TTCGAA	2 cut(s)	1002, 1530
AvaI	CYCGRG	1 cut(s)	1784
AvaII	GGWCC	5 cut(s)	132, 291, 934, 1040, 1132
BaeGI	GKGCMC	2 cut(s)	1580, 1951
BanI	GGYRCC	3 cut(s)	60, 1211, 1946
BanII	GRGCYC	2 cut(s)	22, 874
BarI	GAAGNNNNNNTAC	2 cut(s)	1856, 1888
BbsI	GAAGAC	4 cut(s)	339, 1575, 1686, 1875
Bbv12I	GWGCWC	3 cut(s)	22, 42, 916
BbvI	GCAGC	4 cut(s)	244, 284, 644, 1150
BccI	CCATC	8 cut(s)	220, 383, 416, 647, 956, 1154, 1610, 1922
BceAI	ACGGC	4 cut(s)	504, 509, 1886, 1910
BcgI	CGANNNNNNTGC	4 cut(s)	1444, 1478, 1520, 1554
BciVI	GTATCC	1 cut(s)	1559
BcoDI	GTCTC	7 cut(s)	235, 304, 762, 1414, 1445, 1628, 1718
BfaI	CTAG	2 cut(s)	609, 996
BfoI	RGCGCY	1 cut(s)	462
BfrI	CTTAAG	1 cut(s)	1145
BfuI	GTATCC	1 cut(s)	1559
BisI	GCNGC	6 cut(s)	258, 273, 633, 636, 1139, 1215
BlsI	GCNGC	6 cut(s)	259, 274, 634, 637, 1140, 1216
BmcAI	AGTACT	1 cut(s)	815
Bme18I	GGWCC	5 cut(s)	132, 291, 934, 1040, 1132
BmeRI	GACNNNNNGTC	1 cut(s)	1984
BmeT110I	CYCGRG	1 cut(s)	1784
BmgT120I	GGNCC	5 cut(s)	132, 291, 934, 1040, 1132
BmiI	GGNNCC	8 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1966
BmsI	GCATC	7 cut(s)	511, 544, 568, 1054, 1239, 1528, 1696
BoxI	GACNNNNGTC	3 cut(s)	1406, 1617, 1730
BpiI	GAAGAC	4 cut(s)	339, 1575, 1686, 1875
BpmI	CTGGAG	3 cut(s)	1476, 1742, 1983
Bpu14I	TTCGAA	2 cut(s)	1002, 1530
BpuEI	CTTGAG	2 cut(s)	1255, 1871
Bsa29I	ATCGAT	1 cut(s)	1704
BsaBI	GATNNNNATC	1 cut(s)	1601
BsaI	GGTCTC	1 cut(s)	1718
BsaWI	WCCGGW	1 cut(s)	1481
Bsc4I	CCNNNNNNNGG	8 cut(s)	216, 217, 451, 565, 931, 1178, 1195, 1836
Bse118I	RCCGGY	1 cut(s)	581
Bse3DI	GCAATG	1 cut(s)	78
Bse8I	GATNNNNATC	1 cut(s)	1601
BseCI	ATCGAT	1 cut(s)	1704
BseGI	GGATG	5 cut(s)	427, 526, 583, 678, 1066
BseJI	GATNNNNATC	1 cut(s)	1601
BseLI	CCNNNNNNNGG	8 cut(s)	216, 217, 451, 565, 931, 1178, 1195, 1836
BseMI	GCAATG	1 cut(s)	78
BseMII	CTCAG	5 cut(s)	898, 931, 1639, 1935, 1983
BseRI	GAGGAG	6 cut(s)	1278, 1332, 1500, 1649, 1679, 1988
BseSI	GKGCMC	2 cut(s)	1580, 1951
BseXI	GCAGC	4 cut(s)	244, 284, 644, 1150
BseYI	CCCAGC	1 cut(s)	593
Bsh1236I	CGCG	2 cut(s)	24, 44
Bsh1285I	CGRYCG	1 cut(s)	48
BshNI	GGYRCC	3 cut(s)	60, 1211, 1946
BshVI	ATCGAT	1 cut(s)	1704
BsiEI	CGRYCG	1 cut(s)	48
BsiHKAI	GWGCWC	3 cut(s)	22, 42, 916
BsiHKCI	CYCGRG	1 cut(s)	1784
BsiSI	CCGG	2 cut(s)	582, 1482
BslFI	GGGAC	3 cut(s)	145, 521, 1053
BslI	CCNNNNNNNGG	8 cut(s)	216, 217, 451, 565, 931, 1178, 1195, 1836
BsmAI	GTCTC	7 cut(s)	235, 304, 762, 1414, 1445, 1628, 1718
BsmBI	CGTCTC	1 cut(s)	1628
BsmFI	GGGAC	3 cut(s)	145, 521, 1053
BsmI	GAATGC	2 cut(s)	224, 497
Bso31I	GGTCTC	1 cut(s)	1718
BsoBI	CYCGRG	1 cut(s)	1784
Bsp119I	TTCGAA	2 cut(s)	1002, 1530
Bsp1286I	GDGCHC	6 cut(s)	22, 42, 874, 916, 1580, 1951
Bsp1407I	TGTACA	1 cut(s)	1219
Bsp19I	CCATGG	1 cut(s)	1035
Bsp68I	TCGCGA	1 cut(s)	44
BspACI	CCGC	5 cut(s)	24, 630, 636, 1215, 1815
BspCNI	CTCAG	5 cut(s)	897, 930, 1638, 1934, 1982
BspDI	ATCGAT	1 cut(s)	1704
BspFNI	CGCG	2 cut(s)	24, 44
BspLI	GGNNCC	8 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1966
BspPI	GGATC	5 cut(s)	34, 422, 812, 1401, 1709
BspT104I	TTCGAA	2 cut(s)	1002, 1530
BspT107I	GGYRCC	3 cut(s)	60, 1211, 1946
BspTI	CTTAAG	1 cut(s)	1145
BspTNI	GGTCTC	1 cut(s)	1718
BsrDI	GCAATG	1 cut(s)	78
BsrFI	RCCGGY	1 cut(s)	581
BsrGI	TGTACA	1 cut(s)	1219
BssAI	RCCGGY	1 cut(s)	581
BssNAI	GTATAC	1 cut(s)	1930
BssT1I	CCWWGG	8 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1499
Bst1107I	GTATAC	1 cut(s)	1930
Bst4CI	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
Bst6I	CTCTTC	2 cut(s)	444, 999
BstAFI	CTTAAG	1 cut(s)	1145
BstAUI	TGTACA	1 cut(s)	1219
BstBI	TTCGAA	2 cut(s)	1002, 1530
BstC8I	GCNNGC	8 cut(s)	7, 22, 42, 903, 926, 1069, 1711, 1773
BstDEI	CTNAG	7 cut(s)	261, 474, 884, 917, 1625, 1921, 1969
BstDSI	CCRYGG	1 cut(s)	1035
BstEII	GGTNACC	1 cut(s)	197
BstF5I	GGATG	5 cut(s)	427, 526, 583, 678, 1066
BstFNI	CGCG	2 cut(s)	24, 44
BstH2I	RGCGCY	1 cut(s)	462
BstHHI	GCGC	2 cut(s)	461, 1647
BstMAI	GTCTC	7 cut(s)	235, 304, 762, 1414, 1445, 1628, 1718
BstMCI	CGRYCG	1 cut(s)	48
BstMWI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1996
BstPAI	GACNNNNGTC	3 cut(s)	1406, 1617, 1730
BstPI	GGTNACC	1 cut(s)	197
BstSLI	GKGCMC	2 cut(s)	1580, 1951
BstUI	CGCG	2 cut(s)	24, 44
BstV1I	GCAGC	4 cut(s)	244, 284, 644, 1150
BstV2I	GAAGAC	4 cut(s)	339, 1575, 1686, 1875
BstX2I	RGATCY	1 cut(s)	26
BstYI	RGATCY	1 cut(s)	26
BstZ17I	GTATAC	1 cut(s)	1930
Bsu15I	ATCGAT	1 cut(s)	1704
BsuI	GTATCC	1 cut(s)	1559
BsuTUI	ATCGAT	1 cut(s)	1704
BtgI	CCRYGG	1 cut(s)	1035
BtgZI	GCGATG	1 cut(s)	625
BtsCI	GGATG	5 cut(s)	427, 526, 583, 678, 1066
BtsIMutI	CAGTG	1 cut(s)	241
BtuMI	TCGCGA	1 cut(s)	44
Cac8I	GCNNGC	8 cut(s)	7, 22, 42, 903, 926, 1069, 1711, 1773
CfoI	GCGC	2 cut(s)	461, 1647
Cfr10I	RCCGGY	1 cut(s)	581
Cfr13I	GGNCC	5 cut(s)	132, 291, 934, 1040, 1132
ClaI	ATCGAT	1 cut(s)	1704
CsiI	ACCWGGT	1 cut(s)	930
Csp6I	GTAC	3 cut(s)	814, 1116, 1220
CspCI	CAANNNNNGTGG	2 cut(s)	646, 681
CviAII	CATG	9 cut(s)	170, 216, 355, 463, 808, 1036, 1648, 1741, 1991
CviQI	GTAC	3 cut(s)	814, 1116, 1220
DdeI	CTNAG	7 cut(s)	261, 474, 884, 917, 1625, 1921, 1969
DriI	GACNNNNNGTC	1 cut(s)	1984
Eam1104I	CTCTTC	2 cut(s)	444, 999
Eam1105I	GACNNNNNGTC	1 cut(s)	1984
EarI	CTCTTC	2 cut(s)	444, 999
Ecl136II	GAGCTC	1 cut(s)	20
Eco130I	CCWWGG	8 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1499
Eco24I	GRGCYC	2 cut(s)	22, 874
Eco31I	GGTCTC	1 cut(s)	1718
Eco32I	GATATC	1 cut(s)	1267
Eco47I	GGWCC	5 cut(s)	132, 291, 934, 1040, 1132
Eco53kI	GAGCTC	1 cut(s)	20
Eco57I	CTGAAG	1 cut(s)	513
Eco88I	CYCGRG	1 cut(s)	1784
Eco91I	GGTNACC	1 cut(s)	197
EcoICRI	GAGCTC	1 cut(s)	20
EcoO109I	RGGNCCY	1 cut(s)	1040
EcoO65I	GGTNACC	1 cut(s)	197
EcoRI	GAATTC	1 cut(s)	1485
EcoRV	GATATC	1 cut(s)	1267
EcoT14I	CCWWGG	8 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1499
EcoT38I	GRGCYC	2 cut(s)	22, 874
ErhI	CCWWGG	8 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1499
Esp3I	CGTCTC	1 cut(s)	1628
FaeI	CATG	9 cut(s)	173, 219, 358, 466, 811, 1039, 1651, 1744, 1994
FalI	AAGNNNNNCTT	8 cut(s)	486, 518, 1139, 1171, 1254, 1286, 1755, 1787
FaqI	GGGAC	3 cut(s)	145, 521, 1053
FatI	CATG	9 cut(s)	169, 215, 354, 462, 807, 1035, 1647, 1740, 1990
FauNDI	CATATG	1 cut(s)	646
FblI	GTMKAC	2 cut(s)	1734, 1929
Fnu4HI	GCNGC	6 cut(s)	258, 273, 633, 636, 1139, 1215
FokI	GGATG	5 cut(s)	434, 533, 590, 665, 1073
FriOI	GRGCYC	2 cut(s)	22, 874
Fsp4HI	GCNGC	6 cut(s)	258, 273, 633, 636, 1139, 1215
FspBI	CTAG	2 cut(s)	609, 996
GlaI	GCGC	2 cut(s)	460, 1646
GluI	GCNGC	6 cut(s)	258, 273, 633, 636, 1139, 1215
GsaI	CCCAGC	1 cut(s)	597
GsuI	CTGGAG	3 cut(s)	1476, 1742, 1983
HaeII	RGCGCY	1 cut(s)	462
HapII	CCGG	2 cut(s)	582, 1482
HhaI	GCGC	2 cut(s)	461, 1647
Hin1II	CATG	9 cut(s)	173, 219, 358, 466, 811, 1039, 1651, 1744, 1994
Hin6I	GCGC	2 cut(s)	459, 1645
HinP1I	GCGC	2 cut(s)	459, 1645
HindIII	AAGCTT	3 cut(s)	359, 1331, 1769
HinfI	GANTC	6 cut(s)	950, 999, 1123, 1438, 1787, 1985
HpaII	CCGG	2 cut(s)	582, 1482
Hpy166II	GTNNAC	6 cut(s)	157, 280, 970, 1186, 1735, 1930
Hpy188I	TCNGA	7 cut(s)	33, 320, 348, 979, 1028, 1792, 1972
Hpy188III	TCNNGA	5 cut(s)	43, 307, 886, 1234, 1941
Hpy8I	GTNNAC	6 cut(s)	157, 280, 970, 1186, 1735, 1930
Hpy99I	CGWCG	2 cut(s)	530, 1094
HpyAV	CCTTC	9 cut(s)	343, 475, 655, 1045, 1280, 1681, 1687, 1792, 1858
HpyCH4III	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
HpyCH4IV	ACGT	2 cut(s)	1557, 1731
HpyCH4V	TGCA	4 cut(s)	71, 542, 1455, 1908
HpyF10VI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1996
HpyF3I	CTNAG	7 cut(s)	261, 474, 884, 917, 1625, 1921, 1969
HpySE526I	ACGT	2 cut(s)	1557, 1731
Hsp92II	CATG	9 cut(s)	173, 219, 358, 466, 811, 1039, 1651, 1744, 1994
HspAI	GCGC	2 cut(s)	459, 1645
LmnI	GCTCC	4 cut(s)	1168, 1291, 1501, 1964
Lsp1109I	GCAGC	4 cut(s)	244, 284, 644, 1150
LweI	GCATC	7 cut(s)	511, 544, 568, 1054, 1239, 1528, 1696
MabI	ACCWGGT	1 cut(s)	930
MaeI	CTAG	2 cut(s)	609, 996
MaeII	ACGT	2 cut(s)	1557, 1731
MaeIII	GTNAC	4 cut(s)	49, 197, 529, 1522
MfeI	CAATTG	1 cut(s)	729
MflI	RGATCY	1 cut(s)	26
MhlI	GDGCHC	6 cut(s)	22, 42, 874, 916, 1580, 1951
MluCI	AATT	5 cut(s)	120, 177, 729, 1469, 1485
MlyI	GAGTC	3 cut(s)	1447, 1796, 1994
MmeI	TCCRAC	3 cut(s)	1294, 1382, 1821
MseI	TTAA	6 cut(s)	381, 831, 1146, 1554, 1653, 2002
MslI	CAYNNNNRTG	2 cut(s)	58, 683
MspA1I	CMGCKG	2 cut(s)	275, 1217
MspCI	CTTAAG	1 cut(s)	1145
MspI	CCGG	2 cut(s)	582, 1482
MunI	CAATTG	1 cut(s)	729
Mva1269I	GAATGC	2 cut(s)	224, 497
MvnI	CGCG	2 cut(s)	24, 44
MwoI	GCNNNNNNNGC	5 cut(s)	641, 902, 911, 1211, 1996
NcoI	CCATGG	1 cut(s)	1035
NdeI	CATATG	1 cut(s)	646
NlaIII	CATG	9 cut(s)	173, 219, 358, 466, 811, 1039, 1651, 1744, 1994
NlaIV	GGNNCC	8 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1966
NmeAIII	GCCGAG	3 cut(s)	922, 1699, 1885
NmuCI	GTSAC	2 cut(s)	49, 1522
NruI	TCGCGA	1 cut(s)	44
NspV	TTCGAA	2 cut(s)	1002, 1530
PaeR7I	CTCGAG	1 cut(s)	1784
PctI	GAATGC	2 cut(s)	224, 497
PfeI	GAWTC	3 cut(s)	950, 999, 1123
PkrI	GCNGC	6 cut(s)	259, 274, 634, 637, 1140, 1216
Ple19I	CGATCG	1 cut(s)	48
PleI	GAGTC	3 cut(s)	1446, 1795, 1993
PpsI	GAGTC	3 cut(s)	1446, 1795, 1993
PpuMI	RGGWCCY	1 cut(s)	1040
PshAI	GACNNNNGTC	3 cut(s)	1406, 1617, 1730
Psp124BI	GAGCTC	1 cut(s)	22
Psp5II	RGGWCCY	1 cut(s)	1040
PspEI	GGTNACC	1 cut(s)	197
PspFI	CCCAGC	1 cut(s)	593
PspN4I	GGNNCC	8 cut(s)	62, 133, 1041, 1170, 1213, 1460, 1948, 1966
PspPI	GGNCC	5 cut(s)	132, 291, 934, 1040, 1132
PspPPI	RGGWCCY	1 cut(s)	1040
PspXI	VCTCGAGB	1 cut(s)	1784
PsuI	RGATCY	1 cut(s)	26
PvuI	CGATCG	1 cut(s)	48
PvuII	CAGCTG	1 cut(s)	275
RruI	TCGCGA	1 cut(s)	44
RsaI	GTAC	3 cut(s)	815, 1117, 1221
RsaNI	GTAC	3 cut(s)	814, 1116, 1220
RseI	CAYNNNNRTG	2 cut(s)	58, 683
SacI	GAGCTC	1 cut(s)	22
SaqAI	TTAA	6 cut(s)	381, 831, 1146, 1554, 1653, 2002
SatI	GCNGC	6 cut(s)	258, 273, 633, 636, 1139, 1215
Sau96I	GGNCC	5 cut(s)	132, 291, 934, 1040, 1132
ScaI	AGTACT	1 cut(s)	815
SchI	GAGTC	3 cut(s)	1447, 1796, 1994
SduI	GDGCHC	6 cut(s)	22, 42, 874, 916, 1580, 1951
SexAI	ACCWGGT	1 cut(s)	930
SfaNI	GCATC	7 cut(s)	511, 544, 568, 1054, 1239, 1528, 1696
Sfr274I	CTCGAG	1 cut(s)	1784
SfuI	TTCGAA	2 cut(s)	1002, 1530
SinI	GGWCC	5 cut(s)	132, 291, 934, 1040, 1132
SlaI	CTCGAG	1 cut(s)	1784
SmiMI	CAYNNNNRTG	2 cut(s)	58, 683
SmlI	CTYRAG	4 cut(s)	1145, 1234, 1784, 1886
SmoI	CTYRAG	4 cut(s)	1145, 1234, 1784, 1886
Sse9I	AATT	5 cut(s)	120, 177, 729, 1469, 1485
SsiI	CCGC	5 cut(s)	24, 630, 636, 1215, 1815
SspMI	CTAG	2 cut(s)	609, 996
SstI	GAGCTC	1 cut(s)	22
StyI	CCWWGG	8 cut(s)	432, 502, 573, 694, 874, 1035, 1128, 1499
TaaI	ACNGT	9 cut(s)	112, 203, 236, 295, 535, 1312, 1351, 1408, 1547
TaiI	ACGT	2 cut(s)	1560, 1734
TaqI	TCGA	9 cut(s)	711, 960, 1002, 1089, 1518, 1530, 1704, 1785, 1833
TasI	AATT	5 cut(s)	120, 177, 729, 1469, 1485
TatI	WGTACW	2 cut(s)	813, 1219
TauI	GCSGC	2 cut(s)	638, 1217
TfiI	GAWTC	3 cut(s)	950, 999, 1123
Tru1I	TTAA	6 cut(s)	381, 831, 1146, 1554, 1653, 2002
Tru9I	TTAA	6 cut(s)	381, 831, 1146, 1554, 1653, 2002
TscAI	CASTG	1 cut(s)	241
TseFI	GTSAC	2 cut(s)	49, 1522
TseI	GCWGC	4 cut(s)	257, 272, 632, 1138
Tsp45I	GTSAC	2 cut(s)	49, 1522
TspDTI	ATGAA	5 cut(s)	371, 494, 636, 808, 1757
TspGWI	ACGGA	2 cut(s)	858, 926
TspRI	CASTG	1 cut(s)	241
Vha464I	CTTAAG	1 cut(s)	1145
VpaK11BI	GGWCC	5 cut(s)	132, 291, 934, 1040, 1132
XapI	RAATTY	2 cut(s)	1469, 1485
XhoI	CTCGAG	1 cut(s)	1784
XmiI	GTMKAC	2 cut(s)	1734, 1929
XspI	CTAG	2 cut(s)	609, 996
ZrmI	AGTACT	1 cut(s)	815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.