MD06G1174200.v1.1
MYB Family

Transcription factor WER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005570
Physical Location & Seq
Reverse (-)
31441554 .. 31443721
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1174200.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGAAGGTGGGGGGAATGAGTATAAGAAAGGTCTTTGGACAGTGGAGGAAGACAGAATTCTAATGGATTATATCAGGGAGCATGGCAAAGGCAAATGGAATCGTGTCAATAAAGTCACAGGATTGAAGAGGTGCGGCAAAAGTTGCAGATTAAGGTGGATGAACTATCTAAGTCCCAACGTGAAGAGAGGTGACTTCTCTGAGGAAGAAGATGACCTCATCATTCGACTCCATAAACTCCTTGGCAACAGATGGTCCTTGATTGCGGGACGTGTTCCTGGAAGAACTGACAACCAAGTCAAGAACCATTGGAACACTCATTTGAGCAAGAAGCTTGGTGTCAATTCAAAGAAGGGAAAGACCAAAGCCAAGACTTATCCCGACTTAGAACGAGCAAAAAAGAACTCCTGCACGCCCTCGTCAAACTCAAACTCCGAGTTGCAGCCACTTCCCAATTCTGATTCCAATTCTAATATATTTGGTGATGATGAAGTTGCAGCTGCCATTGATGGATTTGATGATGAGATAATGAACATCAAGGACAATAATATTGGCACTAAAAGTGGGGTTGGTTTTAGCGACATGTGGGAGGCGATGATGATTGGCAATGACCTTAATCTATATAGCTCTTATTTATTGGACCCTACTCTGGATGATCACTACCCTTTTCAATTTGATCATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

229

Amino Acids

25.94

Weight (kDa)

7.01

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 10 - 57 1.3e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 13 - 71 1.5e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 63 - 108 1.8e-19 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 66 - 111 2.2e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AciI CCGC 2 cut(s) 135, 266
AcsI RAATTY 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 649
AflIII ACRYGT 2 cut(s) 271, 582
AgsI TTSAA 3 cut(s) 127, 348, 671
AjiI CACGTC 1 cut(s) 272
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 3 cut(s) 334, 500, 627
AluI AGCT 3 cut(s) 334, 500, 627
ApeKI GCWGC 3 cut(s) 442, 497, 500
ApoI RAATTY 1 cut(s) 57
AspS9I GGNCC 2 cut(s) 255, 640
AsuHPI GGTGA 2 cut(s) 203, 494
AvaII GGWCC 2 cut(s) 255, 640
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 454, 487, 509
BccI CCATC 2 cut(s) 246, 503
BciT130I CCWGG 1 cut(s) 279
BclI TGATCA 2 cut(s) 655, 676
BisI GCNGC 4 cut(s) 136, 443, 498, 501
BlsI GCNGC 4 cut(s) 137, 444, 499, 502
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 2 cut(s) 255, 640
BmgBI CACGTC 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 255, 640
BmiI GGNNCC 1 cut(s) 642
BmrFI CCNGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse3DI GCAATG 1 cut(s) 613
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 2 cut(s) 165, 658
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 454, 487, 509
BsgI GTGCAG 1 cut(s) 394
BslFI GGGAC 2 cut(s) 159, 282
BslI CCNNNNNNNGG 1 cut(s) 649
BsmFI GGGAC 2 cut(s) 159, 282
Bsp143I GATC 2 cut(s) 655, 676
BspACI CCGC 2 cut(s) 135, 266
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 613
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 2 cut(s) 655, 676
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 2 cut(s) 122, 179
BstAPI GCANNNNNTGC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 3 cut(s) 170, 201, 385
BstF5I GGATG 2 cut(s) 165, 658
BstKTI GATC 2 cut(s) 658, 679
BstMBI GATC 2 cut(s) 655, 676
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 3 cut(s) 454, 487, 509
BstV2I GAAGAC 1 cut(s) 57
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 272
BtsCI GGATG 2 cut(s) 165, 658
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 255, 640
CviAII CATG 2 cut(s) 83, 583
CviJI RGCY 5 cut(s) 334, 368, 445, 500, 627
CviKI_1 RGCY 5 cut(s) 334, 368, 445, 500, 627
DdeI CTNAG 3 cut(s) 170, 201, 385
DpnI GATC 2 cut(s) 657, 678
DpnII GATC 2 cut(s) 655, 676
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 2 cut(s) 122, 179
EarI CTCTTC 2 cut(s) 122, 179
Eco130I CCWWGG 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 255, 640
EcoRI GAATTC 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 2 cut(s) 86, 586
FaiI YATR 8 cut(s) 24, 72, 84, 234, 476, 584, 622, 624
FaqI GGGAC 2 cut(s) 159, 282
FatI CATG 2 cut(s) 82, 582
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 2 cut(s) 655, 676
Fnu4HI GCNGC 4 cut(s) 136, 443, 498, 501
FokI GGATG 2 cut(s) 172, 665
Fsp4HI GCNGC 4 cut(s) 136, 443, 498, 501
GluI GCNGC 4 cut(s) 136, 443, 498, 501
Hin1II CATG 2 cut(s) 86, 586
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 3 cut(s) 100, 228, 461
HphI GGTGA 2 cut(s) 203, 494
Hpy188I TCNGA 3 cut(s) 202, 436, 460
Hpy188III TCNNGA 3 cut(s) 301, 380, 650
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 180, 271
HpyCH4V TGCA 4 cut(s) 147, 411, 442, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 3 cut(s) 170, 201, 385
HpySE526I ACGT 2 cut(s) 180, 271
Hsp92II CATG 2 cut(s) 86, 586
Ksp22I TGATCA 2 cut(s) 655, 676
Kzo9I GATC 2 cut(s) 655, 676
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 6 cut(s) 61, 105, 264, 291, 421, 635
Lsp1109I GCAGC 3 cut(s) 454, 487, 509
MaeII ACGT 2 cut(s) 180, 271
MaeIII GTNAC 2 cut(s) 115, 191
MalI GATC 2 cut(s) 657, 678
MboI GATC 2 cut(s) 655, 676
MboII GAAGA 6 cut(s) 62, 139, 196, 218, 221, 294
MluCI AATT 5 cut(s) 57, 343, 454, 466, 671
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 7 cut(s) 40, 123, 182, 196, 227, 427, 583
MseI TTAA 2 cut(s) 152, 615
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 144
NdeII GATC 2 cut(s) 655, 676
NlaIII CATG 2 cut(s) 86, 586
NlaIV GGNNCC 1 cut(s) 642
NmuCI GTSAC 2 cut(s) 115, 191
NspI RCATGY 1 cut(s) 586
PciI ACATGT 1 cut(s) 582
PfeI GAWTC 2 cut(s) 100, 461
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 4 cut(s) 137, 444, 499, 502
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PscI ACATGT 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 2 cut(s) 255, 640
PvuII CAGCTG 1 cut(s) 500
SaqAI TTAA 2 cut(s) 152, 615
SatI GCNGC 4 cut(s) 136, 443, 498, 501
Sau3AI GATC 2 cut(s) 655, 676
Sau96I GGNCC 2 cut(s) 255, 640
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 279
SinI GGWCC 2 cut(s) 255, 640
Sse9I AATT 5 cut(s) 57, 343, 454, 466, 671
SsiI CCGC 2 cut(s) 135, 266
SspI AATATT 1 cut(s) 550
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 2 cut(s) 183, 274
TaqI TCGA 1 cut(s) 226
TasI AATT 5 cut(s) 57, 343, 454, 466, 671
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 2 cut(s) 100, 461
Tru1I TTAA 2 cut(s) 152, 615
Tru9I TTAA 2 cut(s) 152, 615
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 2 cut(s) 115, 191
TseI GCWGC 3 cut(s) 442, 497, 500
Tsp45I GTSAC 2 cut(s) 115, 191
TspDTI ATGAA 3 cut(s) 176, 504, 545
TspRI CASTG 1 cut(s) 48
VpaK11BI GGWCC 2 cut(s) 255, 640
XapI RAATTY 1 cut(s) 57
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.