MD14G1180700.v1.1
MYB Family

Transcription factor WER-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005570
Physical Location & Seq
Reverse (-)
27344474 .. 27346031
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1180700.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGGAGGTGGGAATGAGTATAAGAAAGGTCTTTGGACAGTGGAGGAGGACAAAATTCTGACGGATTACATCACGTTGCATGGCAAGGGCAAGTGGAATCGTGTCAATAAAGTCTCAGGATTGAAGAGAAGCGGCAAAAGTTGCAGATTAAGGTGGATGAATTATCTAAGTCCCAGCGTGAAGAGAAGTGACTTCTCGGAGGAAGAAGATGATCTCATCATTCGACTCCATAACCTCCTTGGCAACAGGTGGTCTTTGATTGCTGGCCGTGTTCCTGGGAGAACCGACAATCAAGTCAAGAACCATTGGAACGGTCATTTGAGCAAGAAGCTCGGTGTCGGTTCAAAAAGAGGAAAGACAAAAGCGAAGACTTATTCCGACTCAAAACGAGCAAAAAATAATTTCTGCATGCCCTCATCAGAGTCAAACTCCGAGCTGCAACCACTTCCCAATTCCGATTCCAATTCCAAAATTTTCGGTGATCATGAAGCTGCAGCTGCCATTGATGAATTTGATGATGAGATGATGAACATCCAGGACCACATTGGCTCTACAAGTGAGGTTGGTTTTAGTGGCATGTGGGAGGCGACGATGATGAGTAATGACAAATTTTGGTTTCACAGTGATGACCTTAATCAATATAGCCCTTATTTATGGGACCCTACGCTGGATGATCACTACCCTTTTCAATTTGATAATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

235

Amino Acids

26.76

Weight (kDa)

6.45

Isoelectric Point (pI)

58.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 9 - 56 1.6e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 12 - 71 2.3e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 62 - 107 9.5e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 65 - 110 7.8e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AciI CCGC 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 4 cut(s) 54, 471, 509, 608
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 124, 345, 689
AjnI CCWGG 2 cut(s) 274, 534
AluBI AGCT 4 cut(s) 331, 436, 491, 497
AluI AGCT 4 cut(s) 331, 436, 491, 497
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 4 cut(s) 436, 491, 494, 497
ApoI RAATTY 4 cut(s) 54, 471, 509, 608
AspS9I GGNCC 2 cut(s) 538, 658
AsuHPI GGTGA 1 cut(s) 491
AvaII GGWCC 2 cut(s) 538, 658
BbsI GAAGAC 1 cut(s) 374
BbvI GCAGC 4 cut(s) 423, 478, 484, 506
BceAI ACGGC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 276, 536
BclI TGATCA 2 cut(s) 481, 673
BcoDI GTCTC 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 5 cut(s) 133, 437, 492, 495, 498
BlsI GCNGC 5 cut(s) 134, 438, 493, 496, 499
Bme1390I CCNGG 2 cut(s) 276, 536
Bme18I GGWCC 2 cut(s) 538, 658
BmgT120I GGNCC 2 cut(s) 538, 658
BmiI GGNNCC 2 cut(s) 659, 660
BmrFI CCNGG 2 cut(s) 276, 536
BpiI GAAGAC 1 cut(s) 374
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BsaBI GATNNNNATC 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 238, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 2 cut(s) 276, 536
BseDI CCNNGG 2 cut(s) 238, 275
BseGI GGATG 3 cut(s) 162, 531, 676
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMII CTCAG 1 cut(s) 129
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 4 cut(s) 423, 478, 484, 506
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 267
BslFI GGGAC 2 cut(s) 156, 671
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 156, 671
BsnI GGCC 1 cut(s) 267
Bsp143I GATC 3 cut(s) 211, 481, 673
BspACI CCGC 1 cut(s) 132
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 1 cut(s) 128
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 659, 660
BspMAI CTGCAG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 238, 275
BssMI GATC 3 cut(s) 211, 481, 673
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 2 cut(s) 276, 536
Bst4CI ACNGT 3 cut(s) 40, 314, 623
Bst6I CTCTTC 2 cut(s) 119, 176
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 265, 410
BstDEI CTNAG 2 cut(s) 115, 167
BstF5I GGATG 3 cut(s) 162, 531, 676
BstKTI GATC 3 cut(s) 214, 484, 676
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 211, 481, 673
BstMWI GCNNNNNNNGC 2 cut(s) 141, 497
BstNI CCWGG 2 cut(s) 276, 536
BstNSI RCATGY 2 cut(s) 412, 580
BstSCI CCNGG 2 cut(s) 274, 534
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 4 cut(s) 423, 478, 484, 506
BstV2I GAAGAC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 3 cut(s) 162, 531, 676
BtsIMutI CAGTG 2 cut(s) 45, 628
Cac8I GCNNGC 2 cut(s) 265, 410
CciI TCATGA 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 538, 658
CviAII CATG 4 cut(s) 80, 409, 485, 577
CviJI RGCY 7 cut(s) 267, 331, 436, 491, 497, 549, 645
CviKI_1 RGCY 7 cut(s) 267, 331, 436, 491, 497, 549, 645
DdeI CTNAG 2 cut(s) 115, 167
DpnI GATC 3 cut(s) 213, 483, 675
DpnII GATC 3 cut(s) 211, 481, 673
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 119, 176
EarI CTCTTC 2 cut(s) 119, 176
Eco130I CCWWGG 1 cut(s) 238
Eco47I GGWCC 2 cut(s) 538, 658
EcoO109I RGGNCCY 1 cut(s) 658
EcoRII CCWGG 2 cut(s) 274, 534
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 4 cut(s) 83, 412, 488, 580
FaiI YATR 8 cut(s) 21, 81, 231, 410, 486, 578, 642, 655
FaqI GGGAC 2 cut(s) 156, 671
FatI CATG 4 cut(s) 79, 408, 484, 576
FbaI TGATCA 2 cut(s) 481, 673
Fnu4HI GCNGC 5 cut(s) 133, 437, 492, 495, 498
FokI GGATG 3 cut(s) 169, 518, 683
Fsp4HI GCNGC 5 cut(s) 133, 437, 492, 495, 498
GluI GCNGC 5 cut(s) 133, 437, 492, 495, 498
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 267
Hin1II CATG 4 cut(s) 83, 412, 488, 580
HinfI GANTC 5 cut(s) 97, 225, 380, 422, 458
HphI GGTGA 1 cut(s) 491
Hpy188I TCNGA 6 cut(s) 60, 199, 379, 421, 433, 457
Hpy188III TCNNGA 3 cut(s) 117, 298, 485
Hpy99I CGWCG 1 cut(s) 592
HpyCH4III ACNGT 3 cut(s) 40, 314, 623
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 5 cut(s) 79, 144, 408, 439, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 497
HpyF3I CTNAG 2 cut(s) 115, 167
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 4 cut(s) 83, 412, 488, 580
KflI GGGWCCC 1 cut(s) 658
Ksp22I TGATCA 2 cut(s) 481, 673
Kzo9I GATC 3 cut(s) 211, 481, 673
LpnPI CCDG 9 cut(s) 102, 187, 232, 249, 261, 288, 521, 548, 653
Lsp1109I GCAGC 4 cut(s) 423, 478, 484, 506
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 1 cut(s) 188
MalI GATC 3 cut(s) 213, 483, 675
MboI GATC 3 cut(s) 211, 481, 673
MboII GAAGA 5 cut(s) 136, 193, 215, 218, 379
MlyI GAGTC 3 cut(s) 219, 374, 431
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 8 cut(s) 37, 40, 193, 245, 344, 424, 553, 577
MseI TTAA 2 cut(s) 149, 633
MslI CAYNNNNRTG 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 497
MspR9I CCNGG 2 cut(s) 276, 536
MvaI CCWGG 2 cut(s) 276, 536
MwoI GCNNNNNNNGC 2 cut(s) 141, 497
NdeII GATC 3 cut(s) 211, 481, 673
NlaIII CATG 4 cut(s) 83, 412, 488, 580
NlaIV GGNNCC 2 cut(s) 659, 660
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 2 cut(s) 412, 580
PaeI GCATGC 1 cut(s) 412
PagI TCATGA 1 cut(s) 484
PfeI GAWTC 2 cut(s) 97, 458
PfoI TCCNGGA 1 cut(s) 534
PkrI GCNGC 5 cut(s) 134, 438, 493, 496, 499
PleI GAGTC 3 cut(s) 219, 374, 430
PpsI GAGTC 3 cut(s) 219, 374, 430
PpuMI RGGWCCY 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 658
Psp6I CCWGG 2 cut(s) 274, 534
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 274, 534
PspN4I GGNNCC 2 cut(s) 659, 660
PspPI GGNCC 2 cut(s) 538, 658
PspPPI RGGWCCY 1 cut(s) 658
PstI CTGCAG 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 2 cut(s) 149, 633
SatI GCNGC 5 cut(s) 133, 437, 492, 495, 498
Sau3AI GATC 3 cut(s) 211, 481, 673
Sau96I GGNCC 2 cut(s) 538, 658
SchI GAGTC 3 cut(s) 219, 374, 431
ScrFI CCNGG 2 cut(s) 276, 536
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 2 cut(s) 538, 658
SmiMI CAYNNNNRTG 1 cut(s) 624
SphI GCATGC 1 cut(s) 412
SsiI CCGC 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 274, 534
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 40, 314, 623
TaiI ACGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 223
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 2 cut(s) 97, 458
Tru1I TTAA 2 cut(s) 149, 633
Tru9I TTAA 2 cut(s) 149, 633
TscAI CASTG 2 cut(s) 45, 628
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 4 cut(s) 436, 491, 494, 497
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 173, 501, 522, 542
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 2 cut(s) 45, 628
VpaK11BI GGWCC 2 cut(s) 538, 658
XapI RAATTY 4 cut(s) 54, 471, 509, 608
XceI RCATGY 2 cut(s) 412, 580
XcmI CCANNNNNNNNNTGG 2 cut(s) 236, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.