MD06G1233700.v1.1
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004135
Physical Location & Seq
Forward (+)
36482899 .. 36487492
4594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1233700.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGCTTCGCGAAAAAGGGCTTTCATCAGAAGCAGCTGCAGGCGGTAGAAGCTGCTTAGCTTTGAATCCGATTCCTGATCTTTCACTCCACATAAGCCCTCCAAACACAAACACAAACAGTGCTCCCTCCTCCATTTGCACCGACCAAGTAACGCCCGATCACGACTCCAGCAGCTGCTTCGACATATGGCGAAGAGACACCGACGAGGATTGCACCAACGGCGGCGCCCTATCCAACAAATCCCACAGCGACAGCTGTATTAGGGCCAGCTACACCAGTTCTTCCCCAGCAGCTGCTGGCGCCGACACTGAGCTTTCCCTCGCGAATCCCTCGGCCTTAGAGGCCGAAAGCGCCTGGAAGAAGAACTATTTCGGCGGTGGAGACGGTTACAATGACGAAGCGAAGAATAGTGGTAATAGTGTAACTAGTAGTAATGGGATTTCGATGCTTGAGAGACTCAAACCCATCAAAGGGATTCCCGTTTACAGTACCAGTAGTACTAATTGCTCGTTTCCTCCTTCGTTTTCCCCTAATCCTGTGTACCAAACGGCGCCTTATCTGTCTAATTCCTTCGGACCGGCAGCACCTGCTCGGAATTGCAGTGGCAGGGGAATGGGAATGGGATTGGGAGGGTTTAACGGGATAACGATGGACAGTCTAAGCCTAGGAGTAGGAGGAGTGCAACAACAACAGCAGCAGCGTCAACGACAACACCATTTTCCGTCGTATAATTTGAATCCTTATTATCCTCAGCAGCTGCAGCAGCATCACTGTGTTGGAGGAGGTGGAAATTACAACGCCGACTTGTCGTCGAATGGTTTCACCATGAGATCGAAATTTACGCCGCCGAAGCTCATAGGGCAAAATATTAATAAGAGGAACATGAGAGCTCCCAGGATGAGATGGACTACTTCTCTTCATGCTCGTTTTGTTCATGCTGTCGAGCTCCTTGGTGGTCATGAAAGGGCTACTCCAAAGTCAGTGTTGGAGCTCATGGACGTCAAGGATCTCACACTTGCTCATGTTAAGAGCCATTTGCAGATGTATAGAACTGTTAAGAACACTGACAAACCTCTAGCTTCCTCAGATGGATCTGGGGATGAAGATCTTTTGTCTATAACGAACCATCATCATAATTCAAATTCAAATGGTTTACTTAACCAAAGAGGAGCCTCAAATGCAACTTTAAACTTAGAGCACGATCATATGGAACACCGCCCTTTTAACAATCTGTGGGGCAACTCCTCCAGGTATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

420

Amino Acids

45.35

Weight (kDa)

7.7

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 299 - 350 2.4e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 595
AatII GACGTC 1 cut(s) 1002
Acc36I ACCTGC 1 cut(s) 595
AccB1I GGYRCC 3 cut(s) 224, 299, 550
AccII CGCG 2 cut(s) 9, 323
AciI CCGC 5 cut(s) 42, 222, 375, 845, 1216
AclWI GGATC 2 cut(s) 1014, 1099
AcsI RAATTY 2 cut(s) 836, 1141
AcyI GRCGYC 4 cut(s) 225, 300, 551, 999
AfaI GTAC 3 cut(s) 490, 499, 542
AfiI CCNNNNNNNGG 2 cut(s) 470, 471
AgsI TTSAA 4 cut(s) 64, 736, 1140, 1146
AhdI GACNNNNNGTC 1 cut(s) 808
AhlI ACTAGT 1 cut(s) 425
AjnI CCWGG 3 cut(s) 353, 893, 1247
Alw21I GWGCWC 5 cut(s) 124, 892, 948, 993, 1200
Alw26I GTCTC 3 cut(s) 189, 375, 448
AlwI GGATC 2 cut(s) 1014, 1099
AlwNI CAGNNNCTG 5 cut(s) 174, 293, 296, 587, 757
AoxI GGCC 3 cut(s) 264, 333, 342
ApoI RAATTY 2 cut(s) 836, 1141
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 2 cut(s) 368, 818
AspA2I CCTAGG 1 cut(s) 664
AspLEI GCGC 4 cut(s) 227, 302, 353, 553
AspS9I GGNCC 2 cut(s) 264, 575
AsuHPI GGTGA 1 cut(s) 814
AvaII GGWCC 1 cut(s) 575
AvrII CCTAGG 1 cut(s) 664
BanI GGYRCC 3 cut(s) 224, 299, 550
BanII GRGCYC 3 cut(s) 892, 948, 993
Bbv12I GWGCWC 5 cut(s) 124, 892, 948, 993, 1200
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 5 cut(s) 473, 643, 897, 1082, 1134
BceAI ACGGC 2 cut(s) 235, 564
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 3 cut(s) 355, 895, 1249
BcoDI GTCTC 3 cut(s) 189, 375, 448
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 3 cut(s) 426, 665, 1076
BfmI CTRYAG 2 cut(s) 36, 758
BfoI RGCGCY 4 cut(s) 228, 303, 354, 554
BfuAI ACCTGC 1 cut(s) 595
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1105
BlnI CCTAGG 1 cut(s) 664
BlpI GCTNAGC 1 cut(s) 55
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 3 cut(s) 355, 895, 1249
Bme18I GGWCC 1 cut(s) 575
BmeRI GACNNNNNGTC 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 264, 575
BmiI GGNNCC 4 cut(s) 226, 301, 552, 1171
BmrFI CCNGG 3 cut(s) 355, 895, 1249
BmsI GCATC 2 cut(s) 435, 775
BpmI CTGGAG 2 cut(s) 151, 1231
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 1104
BsaHI GRCGYC 4 cut(s) 225, 300, 551, 999
BsaJI CCNNGG 4 cut(s) 330, 664, 893, 949
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 471
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 276, 492
Bse8I GATNNNNATC 1 cut(s) 1104
BseBI CCWGG 3 cut(s) 355, 895, 1249
BseDI CCNNGG 4 cut(s) 330, 664, 893, 949
BseGI GGATG 2 cut(s) 903, 1105
BseJI GATNNNNATC 1 cut(s) 1104
BseLI CCNNNNNNNGG 2 cut(s) 470, 471
BseMII CTCAG 3 cut(s) 300, 764, 1098
BseNI ACTGG 2 cut(s) 276, 492
BseRI GAGGAG 5 cut(s) 118, 690, 795, 1182, 1234
BseYI CCCAGC 1 cut(s) 286
Bsh1236I CGCG 2 cut(s) 9, 323
BshFI GGCC 3 cut(s) 266, 335, 344
BshNI GGYRCC 3 cut(s) 224, 299, 550
BsiHKAI GWGCWC 5 cut(s) 124, 892, 948, 993, 1200
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 2 cut(s) 470, 471
BsmAI GTCTC 3 cut(s) 189, 375, 448
BsmBI CGTCTC 1 cut(s) 375
BsnI GGCC 3 cut(s) 266, 335, 344
Bsp1286I GDGCHC 5 cut(s) 124, 892, 948, 993, 1200
Bsp143I GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
Bsp1720I GCTNAGC 1 cut(s) 55
Bsp68I TCGCGA 2 cut(s) 9, 323
BspACI CCGC 5 cut(s) 42, 222, 375, 845, 1216
BspANI GGCC 3 cut(s) 266, 335, 344
BspCNI CTCAG 3 cut(s) 301, 763, 1097
BspFNI CGCG 2 cut(s) 9, 323
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 4 cut(s) 226, 301, 552, 1171
BspMAI CTGCAG 2 cut(s) 40, 762
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 2 cut(s) 1014, 1099
BspT107I GGYRCC 3 cut(s) 224, 299, 550
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 2 cut(s) 276, 492
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 330, 664, 893, 949
BssMI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
BssNI GRCGYC 4 cut(s) 225, 300, 551, 999
BssT1I CCWWGG 2 cut(s) 664, 949
Bst2UI CCWGG 3 cut(s) 355, 895, 1249
Bst4CI ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
Bst6I CTCTTC 2 cut(s) 187, 921
BstACI GRCGYC 4 cut(s) 225, 300, 551, 999
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 3 cut(s) 40, 268, 298
BstDEI CTNAG 7 cut(s) 55, 309, 337, 659, 750, 1084, 1192
BstF5I GGATG 2 cut(s) 903, 1105
BstFNI CGCG 2 cut(s) 9, 323
BstH2I RGCGCY 4 cut(s) 228, 303, 354, 554
BstHHI GCGC 4 cut(s) 227, 302, 353, 553
BstKTI GATC 7 cut(s) 79, 160, 833, 1009, 1094, 1108, 1204
BstMAI GTCTC 3 cut(s) 189, 375, 448
BstMBI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
BstNI CCWGG 3 cut(s) 355, 895, 1249
BstSCI CCNGG 3 cut(s) 353, 893, 1247
BstSFI CTRYAG 2 cut(s) 36, 758
BstUI CGCG 2 cut(s) 9, 323
BstX2I RGATCY 3 cut(s) 1006, 1091, 1105
BstYI RGATCY 3 cut(s) 1006, 1091, 1105
BsuRI GGCC 3 cut(s) 266, 335, 344
BtsCI GGATG 2 cut(s) 903, 1105
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 6 cut(s) 124, 306, 607, 769, 987, 1062
BtuMI TCGCGA 2 cut(s) 9, 323
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 40, 268, 298
CaiI CAGNNNCTG 5 cut(s) 174, 293, 296, 587, 757
CciI TCATGA 1 cut(s) 958
CfoI GCGC 4 cut(s) 227, 302, 353, 553
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 264, 575
CpoI CGGWCCG 1 cut(s) 575
CseI GACGC 1 cut(s) 689
Csp6I GTAC 3 cut(s) 489, 498, 541
CspI CGGWCCG 1 cut(s) 575
CviAII CATG 7 cut(s) 826, 883, 920, 935, 959, 994, 1022
CviQI GTAC 3 cut(s) 489, 498, 541
DdeI CTNAG 7 cut(s) 55, 309, 337, 659, 750, 1084, 1192
DinI GGCGCC 3 cut(s) 226, 301, 552
DpnI GATC 7 cut(s) 78, 159, 832, 1008, 1093, 1107, 1203
DpnII GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
DraI TTTAAA 1 cut(s) 1188
DriI GACNNNNNGTC 1 cut(s) 808
Eam1104I CTCTTC 2 cut(s) 187, 921
Eam1105I GACNNNNNGTC 1 cut(s) 808
EarI CTCTTC 2 cut(s) 187, 921
Ecl136II GAGCTC 3 cut(s) 890, 946, 991
Eco130I CCWWGG 2 cut(s) 664, 949
Eco24I GRGCYC 3 cut(s) 892, 948, 993
Eco47I GGWCC 1 cut(s) 575
Eco53kI GAGCTC 3 cut(s) 890, 946, 991
EcoICRI GAGCTC 3 cut(s) 890, 946, 991
EcoRII CCWGG 3 cut(s) 353, 893, 1247
EcoT14I CCWWGG 2 cut(s) 664, 949
EcoT38I GRGCYC 3 cut(s) 892, 948, 993
EgeI GGCGCC 3 cut(s) 226, 301, 552
EheI GGCGCC 3 cut(s) 226, 301, 552
ErhI CCWWGG 2 cut(s) 664, 949
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
FatI CATG 7 cut(s) 825, 882, 919, 934, 958, 993, 1021
FauNDI CATATG 2 cut(s) 185, 1206
FokI GGATG 2 cut(s) 910, 1112
FriOI GRGCYC 3 cut(s) 892, 948, 993
FspBI CTAG 3 cut(s) 426, 665, 1076
GlaI GCGC 4 cut(s) 226, 301, 352, 552
GsaI CCCAGC 1 cut(s) 290
GsuI CTGGAG 2 cut(s) 151, 1231
HaeII RGCGCY 4 cut(s) 228, 303, 354, 554
HaeIII GGCC 3 cut(s) 266, 335, 344
HapII CCGG 1 cut(s) 578
HgaI GACGC 1 cut(s) 689
HhaI GCGC 4 cut(s) 227, 302, 353, 553
Hin1I GRCGYC 4 cut(s) 225, 300, 551, 999
Hin1II CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
Hin6I GCGC 4 cut(s) 225, 300, 351, 551
HinP1I GCGC 4 cut(s) 225, 300, 351, 551
HincII GTYRAC 1 cut(s) 704
HindII GTYRAC 1 cut(s) 704
HinfI GANTC 7 cut(s) 64, 70, 164, 325, 456, 475, 736
HpaII CCGG 1 cut(s) 578
HphI GGTGA 1 cut(s) 814
Hpy166II GTNNAC 4 cut(s) 484, 541, 704, 1154
Hpy188I TCNGA 5 cut(s) 28, 69, 575, 594, 1087
Hpy188III TCNNGA 5 cut(s) 8, 74, 161, 322, 959
Hpy8I GTNNAC 4 cut(s) 484, 541, 704, 1154
Hpy99I CGWCG 3 cut(s) 206, 727, 814
HpyAV CCTTC 2 cut(s) 528, 580
HpyCH4III ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
HpyCH4IV ACGT 1 cut(s) 999
HpyCH4V TGCA 8 cut(s) 38, 138, 213, 600, 682, 760, 1039, 1181
HpyF3I CTNAG 7 cut(s) 55, 309, 337, 659, 750, 1084, 1192
HpySE526I ACGT 1 cut(s) 999
Hsp92I GRCGYC 4 cut(s) 225, 300, 551, 999
Hsp92II CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
HspAI GCGC 4 cut(s) 225, 300, 351, 551
KasI GGCGCC 3 cut(s) 224, 299, 550
Kzo9I GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
LmnI GCTCC 5 cut(s) 127, 895, 951, 988, 1169
LweI GCATC 2 cut(s) 435, 775
MaeI CTAG 3 cut(s) 426, 665, 1076
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 3 cut(s) 148, 386, 421
MalI GATC 7 cut(s) 78, 159, 832, 1008, 1093, 1107, 1203
MboI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
MboII GAAGA 7 cut(s) 204, 273, 370, 373, 415, 908, 1115
MflI RGATCY 3 cut(s) 1006, 1091, 1105
MhlI GDGCHC 5 cut(s) 124, 892, 948, 993, 1200
MluCI AATT 8 cut(s) 502, 565, 595, 730, 790, 836, 1135, 1141
Mly113I GGCGCC 3 cut(s) 225, 300, 551
MlyI GAGTC 2 cut(s) 158, 450
MmeI TCCRAC 3 cut(s) 258, 757, 966
MroXI GAANNNNTTC 2 cut(s) 368, 818
MseI TTAA 7 cut(s) 636, 870, 1026, 1056, 1158, 1187, 1224
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 5 cut(s) 35, 174, 255, 293, 757
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 3 cut(s) 355, 895, 1249
MvaI CCWGG 3 cut(s) 355, 895, 1249
MvnI CGCG 2 cut(s) 9, 323
NarI GGCGCC 3 cut(s) 225, 300, 551
NdeI CATATG 2 cut(s) 185, 1206
NdeII GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
NlaIII CATG 7 cut(s) 829, 886, 923, 938, 962, 997, 1025
NlaIV GGNNCC 4 cut(s) 226, 301, 552, 1171
NmeAIII GCCGAG 1 cut(s) 311
NruI TCGCGA 2 cut(s) 9, 323
PagI TCATGA 1 cut(s) 958
PaqCI CACCTGC 1 cut(s) 595
PdmI GAANNNNTTC 2 cut(s) 368, 818
PfeI GAWTC 5 cut(s) 64, 70, 325, 475, 736
PleI GAGTC 2 cut(s) 158, 450
PluTI GGCGCC 3 cut(s) 228, 303, 554
PpsI GAGTC 2 cut(s) 158, 450
PshBI ATTAAT 1 cut(s) 870
Psp124BI GAGCTC 3 cut(s) 892, 948, 993
Psp6I CCWGG 3 cut(s) 353, 893, 1247
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 3 cut(s) 353, 893, 1247
PspN4I GGNNCC 4 cut(s) 226, 301, 552, 1171
PspPI GGNCC 2 cut(s) 264, 575
PstI CTGCAG 2 cut(s) 40, 762
PstNI CAGNNNCTG 5 cut(s) 174, 293, 296, 587, 757
PsuI RGATCY 3 cut(s) 1006, 1091, 1105
PvuII CAGCTG 5 cut(s) 35, 174, 255, 293, 757
RruI TCGCGA 2 cut(s) 9, 323
RsaI GTAC 3 cut(s) 490, 499, 542
RsaNI GTAC 3 cut(s) 489, 498, 541
RseI CAYNNNNRTG 1 cut(s) 771
Rsr2I CGGWCCG 1 cut(s) 575
RsrII CGGWCCG 1 cut(s) 575
SacI GAGCTC 3 cut(s) 892, 948, 993
SaqAI TTAA 7 cut(s) 636, 870, 1026, 1056, 1158, 1187, 1224
Sau3AI GATC 7 cut(s) 76, 157, 830, 1006, 1091, 1105, 1201
Sau96I GGNCC 2 cut(s) 264, 575
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 2 cut(s) 158, 450
ScrFI CCNGG 3 cut(s) 355, 895, 1249
SduI GDGCHC 5 cut(s) 124, 892, 948, 993, 1200
SfaNI GCATC 2 cut(s) 435, 775
SfcI CTRYAG 2 cut(s) 36, 758
SfiI GGCCNNNNNGGCC 1 cut(s) 341
SfoI GGCGCC 3 cut(s) 226, 301, 552
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 8 cut(s) 502, 565, 595, 730, 790, 836, 1135, 1141
SsiI CCGC 5 cut(s) 42, 222, 375, 845, 1216
SspDI GGCGCC 3 cut(s) 224, 299, 550
SspI AATATT 1 cut(s) 868
SspMI CTAG 3 cut(s) 426, 665, 1076
SstI GAGCTC 3 cut(s) 892, 948, 993
StyD4I CCNGG 3 cut(s) 353, 893, 1247
StyI CCWWGG 2 cut(s) 664, 949
TaaI ACNGT 6 cut(s) 119, 386, 488, 656, 773, 1054
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 5 cut(s) 180, 443, 812, 833, 942
TasI AATT 8 cut(s) 502, 565, 595, 730, 790, 836, 1135, 1141
TatI WGTACW 1 cut(s) 497
TauI GCSGC 2 cut(s) 225, 847
TfiI GAWTC 5 cut(s) 64, 70, 325, 475, 736
Tru1I TTAA 7 cut(s) 636, 870, 1026, 1056, 1158, 1187, 1224
Tru9I TTAA 7 cut(s) 636, 870, 1026, 1056, 1158, 1187, 1224
TscAI CASTG 6 cut(s) 124, 313, 607, 776, 987, 1069
TspDTI ATGAA 5 cut(s) 12, 908, 923, 975, 1116
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 6 cut(s) 124, 313, 607, 776, 987, 1069
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 2 cut(s) 836, 1141
XmaJI CCTAGG 1 cut(s) 664
XmnI GAANNNNTTC 2 cut(s) 368, 818
XspI CTAG 3 cut(s) 426, 665, 1076
ZraI GACGTC 1 cut(s) 1000
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.