Rroxscaffold_3G00275540
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004135
Physical Location & Seq
Forward (+)
66951228 .. 66956662
5435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275540.1

Sequence Viewer

Length: 1287 bp
ATGCCTCATAAAGGGATTTTGGAGTCTTTGAATCCGATTCCTGATCTTTCACTTCATATAAGCCCTCCAAACAGTGCTCCAAACGAACCTGCAGACTCTGCGGCCTTCAACATATGGCGGTGCAGAGACAACACCGACGTGCACGGCGGCGGTCTAGTCAAATCTCACAGTGACGATGCTGCTACTACTCCACTCGCCGCAGACACTGAGCTTTCCCTTGCAAATCCCACAAGTGCTTCGGAGGCCGAGAGTGACAGCAACTCATGGAAGATGATGAAATCGAGCTATTTCGCAAGAGATCAGCAGCAAGCAGCGGCCGAAAAGCACAACAACATAAGTCACGGCCTTTCCATGCTTGATGTGGCCGAGCTAGGAATCAAACCCATCAAAGGAATTCCAGTTTATAACACCACTCCCGCAGTAGCTAATTGCTCCTTTCCGTTTTCTGCACCTAATTTGGACCCGTCTAGGGATATTTCAGCAGCTGCTAATTCCAAGCTTTGCTTCTACCAAACGCCGCCGCCGTATCTATCTCCTGCTGCACCCTTCAGACCCGGTGCTGCTGCTATGGGAGCAGGAGTAGGAGGAGGGTTTGAGTCCAGGTTTAACGGGATAACTATGGACAGTCTGAGAGTGCCTCAACAACACTTACAGTATCTGAATCATCATCACCATCAACAACAGCAGCTGCTGCAGCATCAACACCAATGTGGAGTTGGGGTTGGGATTGGGATTGGAGCCCATCATCATCATCATCATGGTGATTTCTCTAATACTAACGGGTTCATCATGAGATCTAGATTTACGCCCAAGTCGTATCAGAGTAAGAGGAACATGAGGGCACCCAGAATGCGATGGACCAGCTCCCTCCACGCTCGTTTTGTTCACGCCGTCGAGCTCCTTGGCGGTCATGAAAGGGCGACTCCAAAGTCAGTCTTGGAGCTCATGGATGTCAAAGATCTCACACTTGCTCATGTTAAGAGCCATTTGCAGATGTATAGAACTGTGAAGAACACTGACAAACCTGTAGCTTCCTCAGGTATGATCTTCATTTGTGATATGCATCAAGCTAGAGGAGCATGGGTGCAAGCAAGTTCAAGAGATCTAAATGGCCACTCTCAACATAGAAGTCCTTCTGGAAACCTATTTCATCATCACGAGGGAAGTGATTTTGCCCAATCACGAAGTAGCTTTGTAGATCTCTCAAATGGGGACCTCATCAAAAATCCAAGCTTGGAGTTCAGCTTAGGGCGACCGGACTGGCACAGCAAAGAAGTACTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

428

Amino Acids

46.92

Weight (kDa)

7.48

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 283 - 334 3.2e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
AasI GACNNNNNNGTC 1 cut(s) 928
Acc36I ACCTGC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 841
AcoI YGGCCR 3 cut(s) 315, 363, 1111
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 1 cut(s) 532
AfaI GTAC 1 cut(s) 1278
AfiI CCNNNNNNNGG 3 cut(s) 11, 389, 469
AgsI TTSAA 3 cut(s) 31, 109, 1098
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 1 cut(s) 599
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AleI CACNNNNGTG 2 cut(s) 137, 708
Alw21I GWGCWC 4 cut(s) 79, 144, 900, 945
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 140
AlwNI CAGNNNCTG 6 cut(s) 98, 206, 485, 658, 688, 691
AoxI GGCC 6 cut(s) 102, 243, 315, 343, 363, 1111
ApaLI GTGCAC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 1132
AspS9I GGNCC 3 cut(s) 460, 858, 1213
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 2 cut(s) 662, 773
AvaII GGWCC 3 cut(s) 460, 858, 1213
AxyI CCTNAGG 1 cut(s) 1036
BaeGI GKGCMC 2 cut(s) 144, 844
BalI TGGCCA 1 cut(s) 1113
BanI GGYRCC 1 cut(s) 841
BanII GRGCYC 3 cut(s) 742, 900, 945
BauI CACGAG 1 cut(s) 1157
Bbv12I GWGCWC 4 cut(s) 79, 144, 900, 945
BccI CCATC 4 cut(s) 392, 681, 750, 849
BceAI ACGGC 4 cut(s) 160, 358, 508, 875
BciT130I CCWGG 1 cut(s) 601
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 5 cut(s) 155, 371, 468, 798, 1071
BfmI CTRYAG 3 cut(s) 90, 692, 1026
BfuAI ACCTGC 1 cut(s) 97
BglII AGATCT 4 cut(s) 794, 958, 1102, 1198
BmcAI AGTACT 1 cut(s) 1278
Bme1390I CCNGG 2 cut(s) 555, 601
Bme18I GGWCC 3 cut(s) 460, 858, 1213
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 460, 858, 1213
BmiI GGNNCC 4 cut(s) 462, 739, 843, 1214
BmrFI CCNGG 2 cut(s) 555, 601
BmsI GCATC 3 cut(s) 166, 706, 1072
BplI GAGNNNNNCTC 2 cut(s) 622, 654
Bpu10I CCTNAGC 1 cut(s) 1246
BpuMI CCSGG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 1062
BsaJI CCNNGG 1 cut(s) 901
BsaWI WCCGGW 1 cut(s) 1255
BsaXI ACNNNNNCTCC 6 cut(s) 564, 594, 705, 735, 1068, 1098
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 389, 469
Bse1I ACTGG 2 cut(s) 398, 1265
Bse21I CCTNAGG 1 cut(s) 1036
Bse8I GATNNNNATC 1 cut(s) 1062
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 901
BseGI GGATG 1 cut(s) 955
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 3 cut(s) 11, 389, 469
BseMII CTCAG 3 cut(s) 198, 620, 1050
BseNI ACTGG 2 cut(s) 398, 1265
BseRI GAGGAG 2 cut(s) 600, 1089
BseSI GKGCMC 2 cut(s) 144, 844
BseX3I CGGCCG 1 cut(s) 315
BsgI GTGCAG 3 cut(s) 142, 432, 525
Bsh1285I CGRYCG 2 cut(s) 318, 1256
BshFI GGCC 6 cut(s) 104, 245, 317, 345, 365, 1113
BshNI GGYRCC 1 cut(s) 841
BsiEI CGRYCG 2 cut(s) 318, 1256
BsiHKAI GWGCWC 4 cut(s) 79, 144, 900, 945
BsiSI CCGG 2 cut(s) 555, 1256
BslFI GGGAC 1 cut(s) 1226
BslI CCNNNNNNNGG 3 cut(s) 11, 389, 469
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 1226
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 6 cut(s) 104, 245, 317, 345, 365, 1113
Bsp1286I GDGCHC 6 cut(s) 79, 144, 742, 844, 900, 945
Bsp143I GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
BspANI GGCC 6 cut(s) 104, 245, 317, 345, 365, 1113
BspCNI CTCAG 3 cut(s) 199, 621, 1049
BspHI TCATGA 2 cut(s) 789, 910
BspLI GGNNCC 4 cut(s) 462, 739, 843, 1214
BspMAI CTGCAG 2 cut(s) 94, 696
BspMI ACCTGC 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 841
BsrI ACTGG 2 cut(s) 398, 1265
BssECI CCNNGG 1 cut(s) 901
BssMI GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
BssSI CACGAG 1 cut(s) 1157
BssT1I CCWWGG 1 cut(s) 901
Bst2BI CACGAG 1 cut(s) 1157
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 5 cut(s) 74, 170, 626, 654, 1006
BstAPI GCANNNNNTGC 2 cut(s) 98, 691
BstC8I GCNNGC 2 cut(s) 309, 1089
BstDEI CTNAG 4 cut(s) 207, 629, 1036, 1246
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 955
BstKTI GATC 7 cut(s) 46, 301, 797, 961, 1047, 1105, 1201
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
BstMCI CGRYCG 2 cut(s) 318, 1256
BstMWI GCNNNNNNNGC 6 cut(s) 98, 242, 572, 691, 694, 1076
BstNI CCWGG 1 cut(s) 601
BstSCI CCNGG 2 cut(s) 553, 599
BstSFI CTRYAG 3 cut(s) 90, 692, 1026
BstSLI GKGCMC 2 cut(s) 144, 844
BstX2I RGATCY 4 cut(s) 794, 958, 1102, 1198
BstYI RGATCY 4 cut(s) 794, 958, 1102, 1198
BstZI CGGCCG 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 1036
BsuRI GGCC 6 cut(s) 104, 245, 317, 345, 365, 1113
BtgZI GCGATG 1 cut(s) 868
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 955
BtsIMutI CAGTG 4 cut(s) 79, 175, 204, 1014
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 309, 1089
CaiI CAGNNNCTG 6 cut(s) 98, 206, 485, 658, 688, 691
CciI TCATGA 2 cut(s) 789, 910
Cfr13I GGNCC 3 cut(s) 460, 858, 1213
Csp6I GTAC 1 cut(s) 1277
CviAII CATG 9 cut(s) 264, 352, 758, 790, 835, 911, 946, 974, 1080
CviQI GTAC 1 cut(s) 1277
DdeI CTNAG 4 cut(s) 207, 629, 1036, 1246
DpnI GATC 7 cut(s) 45, 300, 796, 960, 1046, 1104, 1200
DpnII GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
DrdI GACNNNNNNGTC 1 cut(s) 928
DseDI GACNNNNNNGTC 1 cut(s) 928
EaeI YGGCCR 3 cut(s) 315, 363, 1111
EagI CGGCCG 1 cut(s) 315
Ecl136II GAGCTC 2 cut(s) 898, 943
EclXI CGGCCG 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 901
Eco24I GRGCYC 3 cut(s) 742, 900, 945
Eco47I GGWCC 3 cut(s) 460, 858, 1213
Eco52I CGGCCG 1 cut(s) 315
Eco53kI GAGCTC 2 cut(s) 898, 943
Eco57I CTGAAG 1 cut(s) 532
Eco81I CCTNAGG 1 cut(s) 1036
EcoICRI GAGCTC 2 cut(s) 898, 943
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 1213
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 901
EcoT22I ATGCAT 1 cut(s) 1065
EcoT38I GRGCYC 3 cut(s) 742, 900, 945
ErhI CCWWGG 1 cut(s) 901
FaeI CATG 9 cut(s) 267, 355, 761, 793, 838, 914, 949, 977, 1083
FalI AAGNNNNNCTT 5 cut(s) 488, 520, 920, 952, 1263
FaqI GGGAC 1 cut(s) 1226
FatI CATG 9 cut(s) 263, 351, 757, 789, 834, 910, 945, 973, 1079
FauI CCCGC 1 cut(s) 424
FauNDI CATATG 1 cut(s) 113
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 3 cut(s) 742, 900, 945
FspBI CTAG 5 cut(s) 155, 371, 468, 798, 1071
HaeIII GGCC 6 cut(s) 104, 245, 317, 345, 365, 1113
HapII CCGG 2 cut(s) 555, 1256
Hin1II CATG 9 cut(s) 267, 355, 761, 793, 838, 914, 949, 977, 1083
HindIII AAGCTT 2 cut(s) 497, 1231
HinfI GANTC 8 cut(s) 23, 31, 37, 95, 375, 596, 661, 922
HpaII CCGG 2 cut(s) 555, 1256
HphI GGTGA 2 cut(s) 662, 773
Hpy166II GTNNAC 2 cut(s) 142, 886
Hpy188I TCNGA 6 cut(s) 36, 241, 551, 630, 660, 822
Hpy188III TCNNGA 8 cut(s) 41, 790, 798, 911, 1098, 1137, 1157, 1182
Hpy8I GTNNAC 2 cut(s) 142, 886
Hpy99I CGWCG 2 cut(s) 140, 896
HpyAV CCTTC 3 cut(s) 115, 556, 1143
HpyCH4III ACNGT 5 cut(s) 74, 170, 626, 654, 1006
HpyCH4IV ACGT 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 242, 572, 691, 694, 1076
HpyF3I CTNAG 4 cut(s) 207, 629, 1036, 1246
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 9 cut(s) 267, 355, 761, 793, 838, 914, 949, 977, 1083
Kzo9I GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
LmnI GCTCC 8 cut(s) 82, 437, 572, 737, 869, 903, 940, 1076
LweI GCATC 3 cut(s) 166, 706, 1072
MaeI CTAG 5 cut(s) 155, 371, 468, 798, 1071
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 3 cut(s) 170, 251, 338
MalI GATC 7 cut(s) 45, 300, 796, 960, 1046, 1104, 1200
MboI GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
MboII GAAGA 3 cut(s) 280, 1021, 1039
MflI RGATCY 4 cut(s) 794, 958, 1102, 1198
MhlI GDGCHC 6 cut(s) 79, 144, 742, 844, 900, 945
MlsI TGGCCA 1 cut(s) 1113
MluCI AATT 4 cut(s) 393, 427, 454, 490
MluNI TGGCCA 1 cut(s) 1113
MlyI GAGTC 4 cut(s) 32, 89, 605, 916
Mox20I TGGCCA 1 cut(s) 1113
Mph1103I ATGCAT 1 cut(s) 1065
MroXI GAANNNNTTC 1 cut(s) 1132
MscI TGGCCA 1 cut(s) 1113
MseI TTAA 2 cut(s) 606, 978
MslI CAYNNNNRTG 4 cut(s) 137, 708, 756, 759
Msp20I TGGCCA 1 cut(s) 1113
MspA1I CMGCKG 3 cut(s) 314, 485, 688
MspI CCGG 2 cut(s) 555, 1256
MspR9I CCNGG 2 cut(s) 555, 601
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 1 cut(s) 601
MwoI GCNNNNNNNGC 6 cut(s) 98, 242, 572, 691, 694, 1076
NciI CCSGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 113
NdeII GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
NlaIII CATG 9 cut(s) 267, 355, 761, 793, 838, 914, 949, 977, 1083
NlaIV GGNNCC 4 cut(s) 462, 739, 843, 1214
NmeAIII GCCGAG 2 cut(s) 271, 391
NmuCI GTSAC 3 cut(s) 170, 251, 338
NsiI ATGCAT 1 cut(s) 1065
OliI CACNNNNGTG 2 cut(s) 137, 708
PagI TCATGA 2 cut(s) 789, 910
PcsI WCGNNNNNNNCGW 2 cut(s) 521, 812
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 1132
PfeI GAWTC 4 cut(s) 31, 37, 375, 661
PleI GAGTC 4 cut(s) 31, 89, 604, 916
PpsI GAGTC 4 cut(s) 31, 89, 604, 916
PpuMI RGGWCCY 1 cut(s) 1213
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 900, 945
Psp5II RGGWCCY 1 cut(s) 1213
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 4 cut(s) 462, 739, 843, 1214
PspPI GGNCC 3 cut(s) 460, 858, 1213
PspPPI RGGWCCY 1 cut(s) 1213
PstI CTGCAG 2 cut(s) 94, 696
PstNI CAGNNNCTG 6 cut(s) 98, 206, 485, 658, 688, 691
PsuI RGATCY 4 cut(s) 794, 958, 1102, 1198
PvuII CAGCTG 2 cut(s) 485, 688
RsaI GTAC 1 cut(s) 1278
RsaNI GTAC 1 cut(s) 1277
RseI CAYNNNNRTG 4 cut(s) 137, 708, 756, 759
SacI GAGCTC 2 cut(s) 900, 945
SaqAI TTAA 2 cut(s) 606, 978
Sau3AI GATC 7 cut(s) 43, 298, 794, 958, 1044, 1102, 1198
Sau96I GGNCC 3 cut(s) 460, 858, 1213
ScaI AGTACT 1 cut(s) 1278
SchI GAGTC 4 cut(s) 32, 89, 605, 916
ScrFI CCNGG 2 cut(s) 555, 601
SduI GDGCHC 6 cut(s) 79, 144, 742, 844, 900, 945
SfaNI GCATC 3 cut(s) 166, 706, 1072
SfcI CTRYAG 3 cut(s) 90, 692, 1026
SinI GGWCC 3 cut(s) 460, 858, 1213
SmiMI CAYNNNNRTG 4 cut(s) 137, 708, 756, 759
Sse9I AATT 4 cut(s) 393, 427, 454, 490
SspMI CTAG 5 cut(s) 155, 371, 468, 798, 1071
SstI GAGCTC 2 cut(s) 900, 945
StyD4I CCNGG 2 cut(s) 553, 599
StyI CCWWGG 1 cut(s) 901
TaaI ACNGT 5 cut(s) 74, 170, 626, 654, 1006
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 281, 894
TasI AATT 4 cut(s) 393, 427, 454, 490
TatI WGTACW 1 cut(s) 1276
TauI GCSGC 6 cut(s) 104, 150, 200, 317, 520, 523
TfiI GAWTC 4 cut(s) 31, 37, 375, 661
Tru1I TTAA 2 cut(s) 606, 978
Tru9I TTAA 2 cut(s) 606, 978
TscAI CASTG 4 cut(s) 79, 175, 211, 1021
TseFI GTSAC 3 cut(s) 170, 251, 338
Tsp45I GTSAC 3 cut(s) 170, 251, 338
TspDTI ATGAA 6 cut(s) 44, 290, 775, 927, 1039, 1139
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 4 cut(s) 79, 175, 211, 1021
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 3 cut(s) 460, 858, 1213
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 1 cut(s) 393
XbaI TCTAGA 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 2 cut(s) 358, 713
XmnI GAANNNNTTC 1 cut(s) 1132
XspI CTAG 5 cut(s) 155, 371, 468, 798, 1071
ZrmI AGTACT 1 cut(s) 1278
Zsp2I ATGCAT 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.