pycom14g20200
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004135
Physical Location & Seq
Forward (+)
21278354 .. 21281173
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20200.1

Sequence Viewer

Length: 1134 bp
ATGCTTCACGAAAAAGGGTTTTCATCAGAAGCAGCTGCAGGCGGCAGAAGCTGCCTATCTTTGAATCCGATTCCTGATCTTTCACTCCACATAAGCCCTCCAGACACAAACACAAATAGTGCTCCATCCTCCATTTGCACCGACCGAGCAACGCCCGATCACGACTCTGCCAGCTGCTTCAACATATGGCGAAGAGACACCGACGAGGACGATACCAACGGCGGCGCCCTAACCAACAAATCCCACAGCGACAGCTGTATTAGGGCCAGCCACACCAGTTCTTCCCCAGTAGCCGCTGATGCCGACACTGAGCTCTCCCTCGCCAATCCCTCGGCCTCAGAGGCCGAAAGCGCATGGAAGAAGAACTATTTCGGCGGCGGAGACGTTTACCATGACGAAGGAAAGAACAGTAGTAATAGTGTAAGTAGTAGCAATAAGATTCCGTTGCTTGAGAGACTCAACCCCATCAAAGGGATTCCTGTTTACAGTACTAGTAGTACTAATTGCTCGTTTCCTCCTACGTTTTCGCCTAATCCTGTGTACCAAACGGCGCCGTATCTGTCTAATTCCTTCAGACCGGCAGCACCTGCTGGGAATTGTAGTGGCATGGGCATGGGAATTGGAATGGGATTGGGAGGATTTAACGGGATAACGATGGACAGTTTAAGCCTAGGAGTAGGAGGAGTGCAACCACAACAGCCGCAGCATCAACGGCAACACTATTTTCCGTCCTATAATTTGAATCCTCATTACCCTCAGCAGCATCACTGTGTAGGGGGAGGTGGGAATTACAACGCCGACTTGTCGTCCAATGGTTTCATCATGAGATCTAGATTTACGCCTCCGAAGCTCATAGGGCAGAATATCAACAAGAGGAACATGAGAGCTCCCAGGATGCGATGGACTACTTCTCTTCACGCCCGTTTTGTTCATGCTGTCGAGCTCCTTGGTGGTCATGAAAGGGCTACTCCAAAGTCCGTGTTGGAGCTCATGGATGTGAAGGATCTCACACTTGCTCATGTTAAGAGCCATTTGCAGATGTATAGAACCGTTAAGAACACTGACAAACCTCTAGCTTCCTCTGATGGATCTGGGGATGAACGATCTTTTGTCTACAACAAACCATCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

378

Amino Acids

40.62

Weight (kDa)

7.26

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 298 - 349 2.1e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 595
Acc36I ACCTGC 1 cut(s) 595
AccB1I GGYRCC 2 cut(s) 224, 550
AccI GTMKAC 1 cut(s) 1113
AciI CCGC 6 cut(s) 42, 222, 294, 375, 378, 701
AclWI GGATC 2 cut(s) 1011, 1096
AcuI CTGAAG 1 cut(s) 556
AcyI GRCGYC 2 cut(s) 225, 551
AfaI GTAC 3 cut(s) 490, 499, 542
AfiI CCNNNNNNNGG 2 cut(s) 470, 471
AgsI TTSAA 3 cut(s) 64, 181, 742
AhdI GACNNNNNGTC 1 cut(s) 805
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 890
Alw21I GWGCWC 5 cut(s) 124, 315, 889, 945, 990
Alw26I GTCTC 3 cut(s) 189, 375, 448
AlwI GGATC 2 cut(s) 1011, 1096
AlwNI CAGNNNCTG 2 cut(s) 51, 587
AoxI GGCC 3 cut(s) 264, 333, 342
ApeKI GCWGC 7 cut(s) 32, 35, 51, 174, 581, 703, 760
Asp700I GAANNNNTTC 1 cut(s) 368
AspA2I CCTAGG 1 cut(s) 670
AspLEI GCGC 3 cut(s) 227, 353, 553
AspS9I GGNCC 1 cut(s) 264
AvrII CCTAGG 1 cut(s) 670
BanI GGYRCC 2 cut(s) 224, 550
BanII GRGCYC 4 cut(s) 315, 889, 945, 990
Bbv12I GWGCWC 5 cut(s) 124, 315, 889, 945, 990
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 7 cut(s) 22, 38, 44, 161, 593, 715, 772
BccI CCATC 6 cut(s) 133, 473, 649, 894, 1079, 1132
BceAI ACGGC 4 cut(s) 235, 538, 564, 728
BciT130I CCWGG 1 cut(s) 892
BcoDI GTCTC 3 cut(s) 189, 375, 448
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 4 cut(s) 492, 671, 831, 1073
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 2 cut(s) 228, 554
BfuAI ACCTGC 1 cut(s) 595
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 827
BlnI CCTAGG 1 cut(s) 670
BmcAI AGTACT 2 cut(s) 490, 499
Bme1390I CCNGG 1 cut(s) 892
BmeRI GACNNNNNGTC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 226, 552
BmrFI CCNGG 1 cut(s) 892
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 4 cut(s) 289, 715, 772, 885
BmuI ACTGGG 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 470
BsaHI GRCGYC 2 cut(s) 225, 551
BsaJI CCNNGG 4 cut(s) 330, 670, 890, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 471
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 276, 287
BseBI CCWGG 1 cut(s) 892
BseDI CCNNGG 4 cut(s) 330, 670, 890, 946
BseGI GGATG 4 cut(s) 125, 900, 1000, 1102
BseLI CCNNNNNNNGG 2 cut(s) 470, 471
BseMII CTCAG 3 cut(s) 300, 351, 770
BseNI ACTGG 2 cut(s) 276, 287
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 7 cut(s) 22, 38, 44, 161, 593, 715, 772
BseYI CCCAGC 1 cut(s) 590
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 266, 335, 344
BshNI GGYRCC 2 cut(s) 224, 550
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 5 cut(s) 124, 315, 889, 945, 990
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 2 cut(s) 470, 471
BsmAI GTCTC 3 cut(s) 189, 375, 448
BsmBI CGTCTC 1 cut(s) 375
BsnI GGCC 3 cut(s) 266, 335, 344
Bsp1286I GDGCHC 5 cut(s) 124, 315, 889, 945, 990
Bsp143I GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
BspACI CCGC 6 cut(s) 42, 222, 294, 375, 378, 701
BspANI GGCC 3 cut(s) 266, 335, 344
BspCNI CTCAG 3 cut(s) 301, 350, 769
BspHI TCATGA 2 cut(s) 822, 955
BspLI GGNNCC 2 cut(s) 226, 552
BspMAI CTGCAG 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 2 cut(s) 1011, 1096
BspT107I GGYRCC 2 cut(s) 224, 550
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 2 cut(s) 276, 287
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 330, 670, 890, 946
BssMI GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
BssNI GRCGYC 2 cut(s) 225, 551
BssT1I CCWWGG 2 cut(s) 670, 946
Bst2UI CCWGG 1 cut(s) 892
Bst4CI ACNGT 5 cut(s) 410, 488, 662, 770, 1051
Bst6I CTCTTC 2 cut(s) 187, 918
BstACI GRCGYC 2 cut(s) 225, 551
BstAPI GCANNNNNTGC 2 cut(s) 51, 587
BstC8I GCNNGC 3 cut(s) 40, 172, 268
BstDEI CTNAG 3 cut(s) 309, 337, 756
BstF5I GGATG 4 cut(s) 125, 900, 1000, 1102
BstH2I RGCGCY 2 cut(s) 228, 554
BstHHI GCGC 3 cut(s) 227, 353, 553
BstKTI GATC 6 cut(s) 79, 160, 830, 1006, 1091, 1106
BstMAI GTCTC 3 cut(s) 189, 375, 448
BstMBI GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 9 cut(s) 48, 51, 299, 341, 350, 587, 712, 847, 856
BstNI CCWGG 1 cut(s) 892
BstSCI CCNGG 1 cut(s) 890
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 7 cut(s) 22, 38, 44, 161, 593, 715, 772
BstX2I RGATCY 3 cut(s) 827, 1003, 1088
BstYI RGATCY 3 cut(s) 827, 1003, 1088
BsuRI GGCC 3 cut(s) 266, 335, 344
BtgZI GCGATG 1 cut(s) 913
BtsCI GGATG 4 cut(s) 125, 900, 1000, 1102
BtsIMutI CAGTG 3 cut(s) 306, 766, 1059
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 40, 172, 268
CaiI CAGNNNCTG 2 cut(s) 51, 587
CciI TCATGA 2 cut(s) 822, 955
CfoI GCGC 3 cut(s) 227, 353, 553
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 264
Csp6I GTAC 3 cut(s) 489, 498, 541
CviQI GTAC 3 cut(s) 489, 498, 541
DdeI CTNAG 3 cut(s) 309, 337, 756
DinI GGCGCC 2 cut(s) 226, 552
DpnI GATC 6 cut(s) 78, 159, 829, 1005, 1090, 1105
DpnII GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
DriI GACNNNNNGTC 1 cut(s) 805
Eam1104I CTCTTC 2 cut(s) 187, 918
Eam1105I GACNNNNNGTC 1 cut(s) 805
EarI CTCTTC 2 cut(s) 187, 918
EciI GGCGGA 1 cut(s) 393
Ecl136II GAGCTC 4 cut(s) 313, 887, 943, 988
Eco130I CCWWGG 2 cut(s) 670, 946
Eco24I GRGCYC 4 cut(s) 315, 889, 945, 990
Eco53kI GAGCTC 4 cut(s) 313, 887, 943, 988
Eco57I CTGAAG 1 cut(s) 556
EcoICRI GAGCTC 4 cut(s) 313, 887, 943, 988
EcoRII CCWGG 1 cut(s) 890
EcoT14I CCWWGG 2 cut(s) 670, 946
EcoT38I GRGCYC 4 cut(s) 315, 889, 945, 990
EgeI GGCGCC 2 cut(s) 226, 552
EheI GGCGCC 2 cut(s) 226, 552
ErhI CCWWGG 2 cut(s) 670, 946
Esp3I CGTCTC 1 cut(s) 375
FauNDI CATATG 1 cut(s) 185
FblI GTMKAC 1 cut(s) 1113
FokI GGATG 4 cut(s) 112, 907, 1007, 1109
FriOI GRGCYC 4 cut(s) 315, 889, 945, 990
FspBI CTAG 4 cut(s) 492, 671, 831, 1073
GlaI GCGC 3 cut(s) 226, 352, 552
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 2 cut(s) 228, 554
HaeIII GGCC 3 cut(s) 266, 335, 344
HapII CCGG 1 cut(s) 578
HhaI GCGC 3 cut(s) 227, 353, 553
Hin1I GRCGYC 2 cut(s) 225, 551
Hin6I GCGC 3 cut(s) 225, 351, 551
HinP1I GCGC 3 cut(s) 225, 351, 551
HinfI GANTC 7 cut(s) 64, 70, 164, 439, 456, 475, 742
HpaII CCGG 1 cut(s) 578
Hpy166II GTNNAC 4 cut(s) 388, 484, 541, 1114
Hpy188I TCNGA 6 cut(s) 28, 69, 340, 575, 846, 1084
Hpy188III TCNNGA 7 cut(s) 8, 74, 101, 161, 823, 831, 956
Hpy8I GTNNAC 4 cut(s) 388, 484, 541, 1114
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 392, 580, 994
HpyCH4III ACNGT 5 cut(s) 410, 488, 662, 770, 1051
HpyCH4IV ACGT 2 cut(s) 384, 521
HpyCH4V TGCA 4 cut(s) 38, 138, 688, 1036
HpyF10VI GCNNNNNNNGC 9 cut(s) 48, 51, 299, 341, 350, 587, 712, 847, 856
HpyF3I CTNAG 3 cut(s) 309, 337, 756
HpySE526I ACGT 2 cut(s) 384, 521
Hsp92I GRCGYC 2 cut(s) 225, 551
HspAI GCGC 3 cut(s) 225, 351, 551
KasI GGCGCC 2 cut(s) 224, 550
Kzo9I GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
LmnI GCTCC 4 cut(s) 127, 892, 948, 985
Lsp1109I GCAGC 7 cut(s) 22, 38, 44, 161, 593, 715, 772
LweI GCATC 4 cut(s) 289, 715, 772, 885
MaeI CTAG 4 cut(s) 492, 671, 831, 1073
MaeII ACGT 2 cut(s) 384, 521
MalI GATC 6 cut(s) 78, 159, 829, 1005, 1090, 1105
MboI GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
MboII GAAGA 6 cut(s) 204, 273, 370, 373, 905, 1119
MflI RGATCY 3 cut(s) 827, 1003, 1088
MhlI GDGCHC 5 cut(s) 124, 315, 889, 945, 990
MluCI AATT 6 cut(s) 502, 565, 595, 618, 736, 787
Mly113I GGCGCC 2 cut(s) 225, 551
MlyI GAGTC 2 cut(s) 158, 450
MmeI TCCRAC 1 cut(s) 963
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 4 cut(s) 642, 665, 1023, 1053
MslI CAYNNNNRTG 3 cut(s) 611, 768, 995
MspA1I CMGCKG 4 cut(s) 35, 174, 255, 296
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 1 cut(s) 892
MvaI CCWGG 1 cut(s) 892
MwoI GCNNNNNNNGC 9 cut(s) 48, 51, 299, 341, 350, 587, 712, 847, 856
NarI GGCGCC 2 cut(s) 225, 551
NdeI CATATG 1 cut(s) 185
NdeII GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
NlaIV GGNNCC 2 cut(s) 226, 552
NmeAIII GCCGAG 1 cut(s) 311
PagI TCATGA 2 cut(s) 822, 955
PaqCI CACCTGC 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 5 cut(s) 64, 70, 439, 475, 742
PleI GAGTC 2 cut(s) 158, 450
PluTI GGCGCC 2 cut(s) 228, 554
PpsI GAGTC 2 cut(s) 158, 450
Psp124BI GAGCTC 4 cut(s) 315, 889, 945, 990
Psp6I CCWGG 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 1 cut(s) 890
PspN4I GGNNCC 2 cut(s) 226, 552
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 2 cut(s) 51, 587
PsuI RGATCY 3 cut(s) 827, 1003, 1088
PvuII CAGCTG 3 cut(s) 35, 174, 255
RsaI GTAC 3 cut(s) 490, 499, 542
RsaNI GTAC 3 cut(s) 489, 498, 541
RseI CAYNNNNRTG 3 cut(s) 611, 768, 995
SacI GAGCTC 4 cut(s) 315, 889, 945, 990
SaqAI TTAA 4 cut(s) 642, 665, 1023, 1053
Sau3AI GATC 6 cut(s) 76, 157, 827, 1003, 1088, 1103
Sau96I GGNCC 1 cut(s) 264
ScaI AGTACT 2 cut(s) 490, 499
SchI GAGTC 2 cut(s) 158, 450
ScrFI CCNGG 1 cut(s) 892
SduI GDGCHC 5 cut(s) 124, 315, 889, 945, 990
SfaNI GCATC 4 cut(s) 289, 715, 772, 885
SfcI CTRYAG 1 cut(s) 36
SfiI GGCCNNNNNGGCC 1 cut(s) 341
SfoI GGCGCC 2 cut(s) 226, 552
SmiMI CAYNNNNRTG 3 cut(s) 611, 768, 995
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 6 cut(s) 502, 565, 595, 618, 736, 787
SsiI CCGC 6 cut(s) 42, 222, 294, 375, 378, 701
SspDI GGCGCC 2 cut(s) 224, 550
SspMI CTAG 4 cut(s) 492, 671, 831, 1073
SstI GAGCTC 4 cut(s) 315, 889, 945, 990
StyD4I CCNGG 1 cut(s) 890
StyI CCWWGG 2 cut(s) 670, 946
TaaI ACNGT 5 cut(s) 410, 488, 662, 770, 1051
TaiI ACGT 2 cut(s) 387, 524
TaqI TCGA 1 cut(s) 939
TaqII GACCGA 1 cut(s) 159
TasI AATT 6 cut(s) 502, 565, 595, 618, 736, 787
TatI WGTACW 2 cut(s) 488, 497
TauI GCSGC 5 cut(s) 45, 225, 296, 378, 703
TfiI GAWTC 5 cut(s) 64, 70, 439, 475, 742
Tru1I TTAA 4 cut(s) 642, 665, 1023, 1053
Tru9I TTAA 4 cut(s) 642, 665, 1023, 1053
TscAI CASTG 3 cut(s) 313, 773, 1066
TseI GCWGC 7 cut(s) 32, 35, 51, 174, 581, 703, 760
TspDTI ATGAA 6 cut(s) 12, 808, 920, 972, 1113, 1119
TspGWI ACGGA 3 cut(s) 432, 717, 967
TspRI CASTG 3 cut(s) 313, 773, 1066
XbaI TCTAGA 1 cut(s) 830
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 1113
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 4 cut(s) 492, 671, 831, 1073
ZrmI AGTACT 2 cut(s) 490, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.